Objective To investigate the role of protein O-linked mannose β1,2-N-acetylglucosaminyltransferase 1 (POMGnT1) in the pathogenic process of Alzheimer's disease (AD).Methods The expression of POMGnT1 gene was examined in AD model cells (N2a/amyloid-precursor protein (APP) 695swe,n=3) and N2a/wt cells (n=3) using real-time PCR and Western blotting.This expression was also examined in AD model mice (APP/PS1,n=3) and wild-type mice (n=3) using immunofluorescence staining.Amyloid β-protein (Aβ) were examined in POMGnT1-gene-knockout mice (n=3) and wild-type mice (n=3) using immunochemistRy.Results The expression of POMGnT1 gene decreased in mRNA and protein levels in N2a/APP695swe cells compared to N2a/wt cells (mRNA:0.80±0.02 vs 1.00,t=10.52,P＜0.01;protein:0.50±0.02 vs 1.31±0.04,t=18.64,P＜0.01).Immunofluorescence results showed the reduced expression of POMGnT1 protein in neurons of APP/PS1 mice.Immunochemistry results showed more Aβ deposits in POMGnT1-gene-knockout mice (2 months old:0.358±0.014 vs 0.048±0.001,t=22.58,P＜0.01;1 year old:0.266±0.004 vs 0.229±0.003,t=7.771,P=0.002).Conclusion These findings suggest POMGnT1 gene may play an important role in the pathogenic process of AD.

Objective:To discuss the composition and connotation of practical training objectives for nurses specialized in intravenous therapy, and provide guidance and reference for standardizing the practical training of nurses specialized in intravenous therapy.Methods:In this phenomenological analysis in qualitative research, 13 intravenous treatment and nursing experts from Shandong, Zhejiang, Sichuan, and Shaanxi provinces were selected from May to July 2021 for semi-structured interviews. Colaizzi's 7-step method and Nvivo 12.0 were used to organize data and analyze and refine themes.Results:Three themes and 12 subthemes were extracted for the practical training of intravenous therapy nurses, including knowledge objectives, ability objectives, and well-rounded objectives.Conclusions:Attention should be paid to the setting of clinical professional knowledge, skills and comprehensive quality goals for nurses specialized in intravenous therapy, so as to improve the pertinence and timeliness of training, promote the quality of training and the professional development of specialized training for intravenous therapy.

Objective To discuss the influence of the doctor-nurse whole course medical mode on the satisfaction of ICU patients ’ family members.Methods Totals of 200 patients ’ family members were investigated before and after the implementation of the doctor-nurse whole course medical mode.The satisfaction was evaluated before and after the usage of the doctor-nurse whole course medical mode.Results The satisfaction of patients’ family members on nurses before and after the doctor-nurse whole course medical mode was 89.5%and 97.0%, respectively.There was a significant difference (χ2 =8.937,P=0.003).The mean scores of nurses work initiative, nursing technology, service attitude, health education, ward management were (8.43 ±1.30), (8.39 ±1.29), (5.51 ±0.99), (8.26 ±1.64), (5.54 ±1.04) before and (8.94 ± 0.37), (8.85 ±0.70), (5.97 ±0.26), (8.95 ±0.31), (5.95 ±0.31) after the doctor-nurse whole course medical mode.There were significant differences (t=5.336, 4.423, 6.356, 5.847, 5.343, respectively;P<0.01 ) .Conclusions Implementation of the doctor-nurse whole course medical mode can improve the professional quality of nurses and increase the patients’ families satisfaction to nurses job.