Objective To study effect of warfarin combined with lanatoside C on heart function indexes, its curative efficacy in elderly patients with dilated cardiomyopathy, and search for a better therapy for elderly patients with dilated cardiomyopathy.Methods 192 cases of patients with dilated cardiomyopathy were randomly divided into control group and treatment group, 96 cases in each group.Patients in control group were treated with warfarin, and treatment group were given warfarin combined with lanatoside C.After treatment, clinical curative effect evaluation, left ventricular ejection fraction (LVEF), early filling rate/advanced rate (E/A), early peak flow velocity (EPFV), atrium peak flow velocity (APFV), and cardiac indexes related to cardiac volume: left ventricular end systolic volume (LVESV), left ventricular end diastolic volume (LVEDV), left ventricular mass (LV), cardiothoracic ratio (CTR), and 6min walk test were observed and recorded in two groups.Results The total effective rate in treatment group (85.42%) was significantly higher than that of control group (68.75%, P<0.01).The LVEF, E/A, EPFV, APFV in two groups of patients were improved compared with those before treatment (P<0.01), and the improvement of the above indexes in treatment group were significantly better than those in control group (P<0.01).The LVESV, LVEDV, LV, and CTR were improved in two groups after treatment than before treatment (P<0.01), and the improvement of above indexes in treatment group were significantly better than control group after treatment(P<0.05).6min walking test results were improved in two groups after treatment than before treatment ( P<0.05 ) , and the treatment group was significantly better than control group (P<0.05).There were no significant differences between two groups in adverse reactions.Conclusion Warfarin combined with lanatoside C in the treatment of elderly patients with dilated cardiomyopathy is a safe and effective treatment method.

Objective To compare curative effect of chlorthalidone and bisoprolol in treatment of hypertension in the elderly man.Methods 2013 January to 2013 December in our hospital treatment of 60 elderly patients with hypertension were randomly divided into treatment group and control group, each of 30 cases.Treatment group was treated with chlorthalidone tablet, control group was treated with imported bisoprolol tablets.Observed and recorded the two groups before and after treatment the clinical curative effect, blood pressure, fasting blood glucose, uric acid, creatinine, urea nitrogen and adverse reaction.Results The total effective rate of the treatment group ( 90.00%) was significantly higher than that of the control group (73.33%), the difference was statistically significant (P<0.05); compared with before treatment, the treatment group before and after treatment systolic blood pressure and diastolic blood pressure were significantly decreased, the difference was statistically significant (P<0.05);the control group after treatment systolic blood pressure diastolic blood pressure, there was statistical significance between before and after treatment ( P<0.05 );compared with the control group, the treatment group after treatment systolic blood pressure was significantly better than the control group (P<0.05), while the two group of diastolic blood pressure had no significant difference in improving.The two groups before and after treatment, fasting blood glucose, uric acid, creatinine, urea nitrogen were no statistically significant difference.The treatment group and the control group had no obvious adverse reactions.Conclusion The curative effect of chlorthalidone treatment of hypertension in the elderly with good curative effect, few side effects, and beffer than bisoprolol.

The authors reviewed the research and development of tissue engineered meniscus from three points (cell seeds, scaffolds, cell factors) and point out current questions and investigative direction in the future.

0.05), but serum levels of, SS, SP and VIP were higher than those of control group (P05). When the intensity reached 120dB, gastrointestinal transit was accelerated significantly(P

0.05), and the serum levels of MLT, SS, SP, and VIP were higher than those of control group(P0.05). When the noise was higher than 80dB, gastrointestinal transit was increased significantly (P

Objectives:To investigate the changes of portal hemodynamics in patients with liver cirrhosis and its clinical value. Methods:Forty patients with liver cirrhosis and twenty healthy volunteers as controls were involved in this study.The max diameters,mean flow velocity,flow rate and congestion index(CI) of the portal vein trunks(PV) and splenic veins(SV) were detected by Color Doppler Ultrasound in all subjects. Results:The PV diameters(PVD), PVT flow rate(PVF), PVCI and SV diameter(SVD),SV flow rate(SVF),SVCI were significantly highter in patients than in controls( P

Objective To investigate the clinical value of of procalcitonin detection in neonatal necrotizing enterocolitis (NEC).Methods Sixty cases eligible NEC infants were randomly divided into observation group and control group(n=30 each group).All the infants were divided into Ⅰ grade group(19cases),Ⅱ-Ⅲ grade group(41 cases) according to NEC classification.All infants received PCT detection after NEC diagnosis.The observation group received antibiotic treatment base on the serum PCT levels.The control group received antibiotic treatment according to treatment routine and physician's clinical experience.Results PCT increased in 28 (46.7％) of the 60 NEC infants.In the Ⅰ grade group and Ⅱ-m grade group,the proportion of PCT increased cases were 26.3％,61.3％ respectively,and the PCTmax in PCT positive cases were (0.81±0.25) μg/L,(1.76±0.89) μg/L respectively,the differences were signifiacnt(x2 =4.627,t=-2.608,P＜0.05).The time of antibiotic usage in observation group was significantly less than that in control group ((4.9±3.0) d vs.(8.6±2.5) d,t=-3.645,P＜0.01).The fasting time and the cure time of the two groups had no significant difference (P＞ 0.05).Conclusion PCT detection can help to understand the cause and severity of NEC.Using antibiotics according to PCT levels can reduce the time of antibiotic usage without affecting the effect of the NEC treatment.

Background and purpose:Exploration of the effective early diagnostic and prognostic markers of non-small cell lung cancer (NSCLC) has important scientiifc signiifcance and clinical value. Recently, the role of long chain non-coding RNA (lncRNA) in the tumor attracts widespread attention. This study intended to investigate the level of lncRNA HOTTIP in NSCLC, the effect of HOTTIP on cell proliferation and its mechanisms.Methods:Expression of lncRNA HOTTIP in tumor and their matched non-tumor tissues were determined by quantitative real-time PCR (qRT-PCR) in NSCLC patients. Then, we analyzed the potential correlation of lncRNA HOTTIP expression levels in tumor tissues with clinicopathological features of NSCLC and clinical outcome. The effects of HOTTIP on NSCLC cell proliferation and apoptosis were tested usingin vitro MTT and lfow cytometric assays. Western blot method was uesd to detect the expressions of proteins.Results:LncRNA HOTTIP expression level was signiifcantly decreased in NSCLC tissues in comparison to adjacent non-tumor tissues (P<0.05). It was also proved that HOTTIP expression was associat-ed with NSCLC histological grade and lymph node metastasis. Moreover, knockdown of HOTTIP expression in A549 cell line decreased proliferation and enhanced apoptosis compared with transfected negative control. Western blot assay showed that the level of Bax protein was signiifcantly increased, whereas Bcl-2 was signiifcantly decreased in HOT-TIP-silencing A549 cell.Conclusion:HOTTIP is a novel prognostic biomarker and a potential therapeutic candidate forNSCLC.

Objective To investigate the role of expression in the differential diagnosis of thyroid follicular carcinoma and follicular variant of papillary carcinoma. Methods Seventy cases of thyroid lesions (including 15 cases of follicular adenomas, 15 cases of adinomatous goiters, 30 cases of papillary carcinomas and 10 cases of follicular carcinomas) were collected, and CD10 expression was detected by means of immunohistochemistry in above thyroid lesions. Results Seven of 9 cases of follicular variant of papillary carcinoma were CD10 positive (77.8), and 8 of 10 cases of follicular carcinoma were CD10 positive (80.0). However, CD10 was negative in all cases of non-follicular variant of papillary carcinoma, follicular adenoma, adinomatous goiter and normal thyroid tissue. Conclusion The detection of CD10 expression is useful to the differential diagnosis of thyroid follicular carcinoma and follicular variant of papillary carcinoma.

Objective To predict and identify T cell epitopes of major group 3 allergen derived from Dermatophagoides fari-na(Der f 3). Methods The T cell epitopes of Der f 3 were analyzed through the sequence analysis by using the bioinformatics online tools. The five predicted peptides of T-cell epitopes were artificially synthesized. The spleen lymphocytes were co-cultured with the five T cell epitopes by using the modified MTT method and the levels of IL-2,IFN-γ,IL-4 and IL-5 in the supernatant of the cultures were detected by ELISA. Results Five T cell epitopes of Der f 3 were predicted and three of which could pro-mote the proliferation of the mouse spleen lymphocytes. The secretions of IL-2 and IFN-γwere significantly induced and the se-cretions of IL-4 and IL-5 were significantly decreased by three of five prediction epitopes of Der f 3:37GDCPYQISLQSSSHFC-GG54,98IYQHENYDSMTIDNDVALIKLKTPMT123 and 164SELQRVDIDVVSREQCDQLYS184. Conclusion Three T cell epitopes of Der f 3 have been initially identified,which lays the foundation of the diagnosis and treatment of asthma.