Objective:To synthesize 177Lu-prostate-specific membrane antigen (PSMA)-617 with domestic 177Lu (made in China), and explore its optimal labeling condition, biodistribution, stability, and safety. Methods:177Lu-PSMA-617 was prepared with domestic 177Lu by a manual method. The optimal labeling condition, radiochemical purity, stability ( in vivo and in vitro), lipid-water partition coefficient, and plasma protein binding rate were determined. The uptake rate of 177Lu-PSMA-617 was evaluated by using 22RV1 cells. Biodistribution and SPECT/CT imaging were performed on normal mice with imported 177Lu-PSMA-617 as control group. The blood routine test was performed to evaluate the safety. Results:The best labeling result of domestic 177Lu-PSMA-617 can be obtained under the following conditions: pH=4.5, 100 ℃ for 30 min. And the radiochemical purity was ≥99%. The product was stable in vivo and in vitro, with the radiochemical purity >95% in 72 h. The plasma protein binding rate was (35.3±5.3)%, the lipid-water partition coefficient was -2.27±0.06, and the specific uptake rate of domestic 177Lu-PSMA-617 by 22RV1 cells reached the highest in 1 h ((7.58±0.84)%), which was slightly lower than the imported 177Lu-PSMA-617 ((7.86±0.96)%), but there was no significant difference between them ( t=-0.439, P>0.05). The distribution and SPECT/CT imaging of normal mice showed that domestic and imported 177Lu-PSMA-617 in blood were cleared quite fast, and both of them were excreted mainly through the kidneys. No obvious adverse reactions were found in the toxicity test of domestic and imported 177Lu-PSMA-617. There was no obvious abnormality in blood routine and liver and kidney metabolism. Conclusion:The domestic 177Lu-PSMA-617 has many advantages, such as qualified quality control, good biological properties and safety, which support its potential application value in diagnosis of prostatic neoplasms.

Most of the CHD patients reveal string pulse, mainly due to damage of heart function, lowering of arterial compliance and increase of total peripheral resistance. The common pulse in patients of blood diseases reveal frequent, tiny, string and slippery characteristic, mostly due to the increase of compensatory pumping action of the heart, shortening of ejection time of the left heart, with better vessel compliance and hemorheology, low total peripheral resistance.

Objective:To improve the health education program for patients with ulcerative colitis (UC) through action research method to explore its effect on disease knowledge and self-nursing ability of patients.Methods:In October 2018, 72 UC patients who were treated in Outpatient Department of Gastroenterology in the First Affiliated Hospital of Zhengzhou University in the past 3 months were selected as research objects by the convenient sampling method. According to the random number table, they were divided into the control group and the observation group, with 36 cases in each group. The control group was given routine health education, while the observation group used the action research method as the theoretical basis to continuously improve and implement health education strategies. Before the intervention and 4 months after the intervention, mastery of disease knowledge and self-nursing ability of patients in the two groups were compared.Results:There was no statistically significant difference in the scores of disease knowledge and self-nursing ability between the two groups before intervention ( P>0.05) . After the intervention, the scores of general knowledge, dietary knowledge, treatment knowledge and total score of disease knowledge of the observation group were all higher than those of the control group, and the differences were statistically significant ( P<0.05) . After the intervention, the scores of three dimensions of self-concept, self-nursing responsibility and health knowledge level and total score of self-care ability in the observation group were higher than those of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The health education strategy based on action research method can improve the disease knowledge and self-care ability of patients with ulcerative colitis, which can be applied to clinical practice to improve the quality of nursing.