Objective To investigate the influence of albumin on the expression of angiotensin-eonverting enzyme (ACE) in cultured human proximal tubular ceils (HK-2). Methods HK-2 cells were exposed to 2.5, 5, 10 g/L bovine serum albumin (BSA) for 6 h and 12 h respectively. The expression of ACE in HK-2 cells was detected by real-time RT-PCR and Western blot. Results Compared to the control group, the expression of HK-2 cells ACE mRNA treated for 12 h with different concentrations of BSA (2.5, 5, 10 g/L) significantly increased (P

In this paper,we have made a preliminary analysis on the necessity,main contents and difficulties in teaching medical undergraduates the principles and applications of optogenetics experimental technology.We focused on theory and practice teaching and emphasized on the most important technological principles,including how to achieve selective intervention of specific neural pathways;how to achieve twoway control of cell excitation or inhibition effect and the main process of the experiment.Finally,we have summarized our recent experience in the teaching process,to provide reference for basic medical experiments teaching in related medical colleges,so as to promote optogenetics experimental teaching for undergraduates in domestic medical colleges.