Objective To establish a quality evaluation method for determination of paris saponinⅠ,Ⅱ,Ⅵ,Ⅶ in paridis rhizhma by quantitative analysis of multi-components with single-marker (QAMS).Methods An HPLC method and a Phenomenex Luna C18 column(4.6 mm×250 mm,5 μm)were used.The mobile phase was acetonitrile-water (48:52) at a flow rate of 1.0 mL·min-1.The detection wavelength was 203 nm and column temperature was 25 ℃.Parissaponin Ⅶ was used as the internal reference substance.The relative correlation factors of parissaponinⅠ,Ⅱ,Ⅵ were calculated by standard curve method and QAMS.Results The QAMS method could be used for determination of four saponin components at the same time without significant difference as compared with the results of standard curve method (RSD<2.0%).Conclusion QAMS method is simple and reproducible,which can provide a reference for quality standard revision for paridis rhizhma.

Objective To study whether noggin alone can induce human bone marrow mesenchymal stem cells (MSCs) to differentiate into neural cells for the treatment of neural degenerative diseases.Methods Human bone marrow MSCs were primarily cultured and then divided into control,Ad group of empty adenovirus vector and Ad-noggin group of adenovirus vector with noggin gene.Their morphological changes were observed with fluorescence microscopy and laser confocal microscopy,and the results of 48 h,4,7,10 d were analyzed with statistical methods.Results Both the vector made human bone marrow MSCs carry green fluorescence.Ad group didn’t show any morphological changes.While for Ad-noggin group,a few neural-like cells appeared in 48 h after transfection.The number of such cells was increased,and there were some neurites around the cells after 4 d.The cells amplified greatly after 10 d.Compared to those of 7 d after transfection,the soma and dendritic diameters of Ad-noggin group were significantly increased after 10 d (P

To study the effects of exercise and weight control on bone mineral density (BMD) of girl athletes, dual X-ray and single X-ray absorptiometry were adopted to measure BMD; And serum Vitamin D3, estrogen, cholesterol and alkaline phosphatase levels of subjects were determined. Subjects were 63 students of two age groups (before puberty. 8-9 years old; after puberty: 15-16 years old, respectively) of sports and ordinary schools. The results showed that.. Exercise training is beneficial to increase BMD; Long term weight control had no effect on BMDof athletes in this study; Yet girl athletes after puberty with low estrogen levels had less BMD; Girl judo players with rapid weight loss had higher BMD as compared with the same aged nontraining students.

Objective To analyze the trends of leprosy epidemic and evaluate the effects of its control in Yunnan Province. Methods The epidemiologic data were analyzed. Results There were 50 797 leprosy patients detected in Yunnan during 1954～ 1998 with a high prevalence in china.Among these patients,37 639 were cured,10 447 died,and 1 069 dropped out.There were 1 642 active patients at the end of 1998.It was in 1966 that the prevalence is highest(0.82/1 000) with 18 350 active patients and decreased to 0.04/1 000 in 1998.The incidence was 19.37/100 000 in 1957 and decreased to 0.32/100 000 in 1998.The most numerous patient (4 680) was detected with a rate of 24.45/100 000 in 1958,and the rate decreased to 1.63/100 000 in 1998.In 1960s and 1970s, a lot of leprosy villages were built and a mass survey of leprosy patients was taken.Doctors treated patients with DDS alone at that time. MDT was the first to use in Mengla in 1983 and spread during 1986～ 1989. Conclusion Several measures were taken for detecting and treating patients effectively, and the endemic and incidence decreased by a big margin as well.

Objective: To investigate the reparative dentine formation induced by the recombinant human bone morphorgenetic protein2(rhBMP2) used as a indirect pulp capping agent in dog teeth. Methods: Type V cavities were prepared in 48 teeth of 2 dogs. rhBMP2 with zinc oxide(A) was lined on the bottom of 24 cavities, saline with zinc oxide(B) in 12 and zinc oxide eugentol cement(C) in 12. 0.5 g of tetracycline was orally administered to the dogs twice a day on day 24, 54, 83 and 111. On day 135, the teeth were extracted and processed for the quantitative observation of reparative dentin under a fluorescent microscope .Results: On day 135, the reparative dentine (expressed as "?m" of diameter of area) in group A, B and C was 825.04? 125.46, 315.33? 80.43 and 415.25? 90.75 respectively(P

Objective To investigate the modulation role of autophagy in radiation-induced cell death by detecting the response of src kinase activity and autophagy in HEK293 cells irradiated with different dose of α-particle.Methods HEK293 cells were irradiated by contral group (0 cGy) a low dose group (10 cGy) and high dose group (300 cGy) α-particles.Molecular probe 2',7'-dichlorofluorescin (DCFHDA) was used to detect the cell ROS.The src kinase activity and endogenous protein level of LC3Ⅰ/Ⅱ were monitored by Western Blot.Cell death rate of irradiated cells pretreated with autophagy inducer of rapamycin was tested by flow cytometry.Results Compared with control group,the ratio of LC3Ⅰ/Ⅱ decreased (t =4.07,P ＜ 0.05) and the percentage of cells with GFP-LC3 punctuate dots increased (t =12.29,P ＜0.05) under 10 cGy irradiation,indicating the induction of autophagy.On the contrary,the ratio of LC3Ⅰ/Ⅱ increased (t =2.93,P ＜ 0.05) and the GFP-LC3 morphology had no obvious change under 300 cGy irradiation.The cellular ROS level reached to the maximum value at 4 h postirradiation.Both 10 cGy and 300 cGy irradiation could elevate the ROS level (t =17.93,22.88,P ＜0.05),whereas the amplitude of elevation of 300 cGy irradiation was higher than that of 10 cGy irradiation (t =15.76,22.66,14.22,P ＜ 0.05).Compared with control group,the 419th site of tyrosine residue in src kinase manifested hyper-phosphorylation (t =5.66,P ＜0.05) under 10 cGy irradiation whereas it had hypo-phosphorylation under 300 cGy irradiation (t =4.67,P ＜ 0.05).Treatment of cells with DMSO could partly restore the impact on src kinase activity under high or low dose irradiation.Pre-treating the cells with autophagy inducer rapamycin could reduce cell death under 300 cGy irradiations (t =12.14,P ＜ 0.05).Conclusions High or low dose of α-particles irradiation could inhibit or activate src kinase and autophagy system,respectively.ROS mediated the response of src kinase activity and autophagy system induced by irradiation.Modulation of autophagy could desensitize cell responses to irradiation.

Objective To observe the clinical efficacy of electroacupuncture plus moxibustion in treating infantile cerebral palsy. Method Eighty eligible patients with infantile cerebral palsy were randomized into a treatment group of 42 cases and a control group of 38 cases. The control group was intervened by physiotherapy (PT), while the treatment group was given electroacupuncture at scalp acupoints, body acupuncture, and moxibustion along the Governor Vessel in addition to the treatment given to the control group. The two groups were treated once a day, 6 months in total. The comprehensive ability and the Gross Motor Function Measure (GMFM) were evaluated before and after the interventions in the two groups, and the clinical efficacies were compared.Result After 6-month treatment, the total effective rate was 92.9% in the treatment group versus 78.9% in the control group, and the total effective rate of the treatment group was superior to that of the control group (P<0.05). The motor function and activities of daily living were significantly improved in both groups after the interventions (P<0.05,P<0.01); after the treatment, there were significant differences in comparing the motor function and activities of daily living between the two groups (P<0.05). The GMFM scores of each domain were significantly changed after the treatment in both groups (P<0.05); after the treatment, there were significant differences in comparing the GMFM scores of each domain between the two groups (P<0.05).Conclusion Compared with being used alone, conventional PT can more significantly benefit the improvement of motor function of cerebral palsy patients when electroacupuncture and moxibustion were added.

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Objective To investigate the roles of SENP1 in regulation of biological characteristics of NK cells.Methods Lentivirus-mediated-Senp1-small-hairpinRNA (shRNA) transduction was applied to NK92 cells.The expression of SENP1 in NK92 cells was determined by real-time PCR and Western blot.The proliferation of NK92 cells was detected by CCK-8 assay.The apoptosis of NK92 cells was determined by Annexin Ⅴ and PI labeling.The cytotoxicity of NK92 cells against K562 cells was evaluated by luciferase reporter assay.Results Treatment of NK92 cells with IL-21 resulted in SENP1 upregulation.Lentivirus mediated SENP1 knockdown reduced proliferation and increased apoptosis in NK-92 cells,but SENP1 inhibition had slight impact on the cytotoxic ability of NK92 cells to kill K562 cells.Conclusion SENP1 mediates the regulatory effect of IL-21 on the proliferation and survival of NK92 cells.

Objective To investigate the changes of brain function in moderate and high myopia patients using fractional amplitude of low frequency fluctuation (fALFF),and discuss the correlation between brain function changes and clinical data of patients with myopia.Methods Totally 21 moderate and high myopia patients (myopia group),and 21 healthy volunteers (normal control group) who were matched with myopia patients in age and gender,were selected to take rs-fMRI examination.The difference of fALFF of brain functional activity in patients with myopia and normal controls was compared,and the correlation between the changes of fALFF and clinical data of patients with myopia was analyzed,Results Compared with normal control group,the fALFF values of myopia group in the region of the left inferior frontal gyrus,putamen and right inferior frontal gyrus,putamen and insula were significantly lower (all P ＜ 0.05,AlphaSim corrected).However,in bilateral cingulate gyrus,bilateral anterior cingulate gyrus,left postcentral gyrus,left superior parietal lobule and region,fALFF values were increased (all P ＜ 0.05,AlphaSim corrected).Conclusion Patients with myopia are accompanied by abnormal neuronal activity in many brain areas,which may reflect the dysfunction of language understanding and attention control in myopic patients.