Energy metabolism varies in different periods during the lifetime or in different pathological states. Resting energy expenditure (REE) may increase in some patients with malignant tumors, but may also show no significant difference when compared with healthy people or patients with benign tumors. The alteration of REE may be related to tumor site, pathologic stage, methods of treatment, and/or laboratory tests. The efficacy of a certain therapy may also affect REE in patients with malignant tumors: when a therapy is effective, the value of REE can return normal; however, when the treatment fails, REE will not be improved or become even higher.Mechanism governing the abnormalities of REE in patients with malignant tumors may be associated with the abnormal nutrition metabolism and the regulations of relevant cytokines.

Objective To evaluate the influence of adenomyosis on IVF-ET outcomes in infertile patients with endometriosis.Methods 122 cases were divided into two groups:group A(research group)contained 32 patients in infertile with both adenomyosis and endometriosis;group B(control group) contained 90 patients with endometriosis.The main data measured were response to COH,outcome of IVF-ET.Results Both groups had no significant difference in Gn dosage,number of oocytes obtained,number of fertilization and number of cleavage(all p＞0.05),The clinical pregnancy rate of group A was 21.9%,which was lower than that of group B(30.0%),but there were no statistical difference(P＞0.05).Conclusion Adenomyosis had no adverse effects on IVF-ET outcomes in infertile women with proven endometriosis.

Objective To set up forecasting models of week and ten days for chronic tracheitis.Methods The data of chronic tracheitis incidence and same time meteorological factors in 1998-2000 in Yinchuan,China were collected and analyzed using SAS6.0 statistical software.Results The incidence of chronic tracheitis was closely related with air temperature,air pressure and the other meteorological factors which showed that chronic tracheitis was caused integrated meteorological factors.Based on the correlation analysis,forecasting models of week and ten days for chronic tracheitis using multi-regression method was established.Conclusion Chronic tracheitis may be caused by integrated meteorological factors and the incidence of chronic tracheitis has a positive correlation with air temperature and a negative correlation with air pressure.

Objective To investigate the effect of standardized nursing intervention on airway inflammatory reaction evaluation by testing fractional concentration of exhaled nitric oxide in asthma patients.Methods 62 asthma patients were randomly divided into the intervention group(32 cases)and the control group (30 cases).Two groups were performed FENO determination before and after the treatment.The control group was given routine method for determination,the intervention group was given nursing intervention on the basis of conventional methods.The comfortable situation,satisfaction degree,treatment efficacy were determined between the two groups.Results The comfortable situation,satisfaction degree,treatment efficacy of the intervention group were better than those of the control group.Conclusions Standardized nursing intervention can eliminate the adverse interference in the FENO determination and improve the accuracy of the determination.It can also improve the clinical symptoms of patients with asthma and relieve airway inflammation.This method deserves to be recommended.

OBJECTIVE:To establish a method for simultaneous determination of luteolin and apigenin in Mongolian medicine Scabiosa atropurea. METHODS:HPLC was performed on the column of Diamond C18 with mobile phase of acetonitrile-0.4%phos-phoric acid(34∶66,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 350 nm,column temperature was 30 ℃,and the injection volume was 10 μl. RESULTS:The linear range was 66-396 ng for luteolin(r=0.999 8)and 93-558 ng for apigenin (r=0.999 6);RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 98.15%-101.79%(RSD=1.42%,n=6) and 98.66%-104.05%(RSD=1.81%,n=6),respectively. CONCLUSIONS:The method is simple with good precise,stability and reproducibility,and can be used for the simultaneous determination of luteolin and apigenin in Mongo-lian medicine S. atropurea.

Ricin is a plant toxin isolated from the seed of the castor plant(Ricinus communis). As a typical II ribosome inactivating protein,ricin consists of two polypeptide chains named ricin toxin A chain(RTA)and ricin toxin B chain(RTB),linked via a disulfide bridge. RTB binds to both glycopro?tein and glycolipid at the surface of the target cell and mediates ricin to be endocytosed and transported retro?gradely to the endoplasmic reticulum. After being reduced and retrotranslocated to the cytosol,RTA mediates its toxicity due to its activity of a RNA N- glycosidases. Aside from its main toxic effect of protein synthesis inhibition,ricin also displays other properties that contribute to its toxicity such as inducing apoptosis,cytokine secreting and peroxidation. Ricin is stable and can be easily isolated. It has many routes of intoxication with no specific antidotes. Due to its natural abundance,remarkable toxicity,and the potential to be used in biological warfare as well as terrorist attacks,ricin has been classified as a Category B biothreat agent. Here we reviewed its history as a biothreat agent,constitu?tion,intoxication mechanism,detection methods and the development of specific antitodes.

OBJECTIVE:To establish HPLC fingerprint sectrum of Rhaponticum uniflorum. METHODS:HPLC was performed on the column of Hypersil-ODS with mobile phase of 0.3% phosphoric acid-acetonitrile(gradient elution) at flow rate of 1.0 ml/min,detection wavelength was 220 nm,column temperature was 30 ℃ and volume injection was 10 μl. The luteolin was refer-ence,17 batches of R. uniflorum from different production places was analyzed and similarity evaluation system for chromatograph-ic fingerprint of TCM (2004 A edition)was adopted for the similarity analysis. RESULTS:There were totally 11 common peaks with similarity degree≥0.900 of 17 batches. According to the verification,the fingerprint spectrum and reference fingerprint spec-trum of R. uniflorum had good consistency. CONCLUSIONS:The established method is specific and stable,and can provide refer-ence for the identification and quality control of R. uniflorum.

Objective To explore the method of comfort nursing in patients with nasogastric feeding and observe the influence on enhancement of patients satisfaction degree. Methods Case control study Was used in 162 conscious patients with nasogastric feeding.80 patients from January 2005 to January 2006 were selected as control group and given routine nursing.82 patients from February 2006 to May 2007 were named as the test group and received confort nursing.The satisfaction degree of patients, injury of nasal mucosa, shedding rate of the gastric tube and aspiration rate were investigated with self-designed discomfort questionnaires. Results The satisfaction degree in the test group Was 63.41％,which was higher than that of the control(40.00％,P＜0.01).The rate of nasal mucosa injury, shedding of the gastric tube and aspiration rate were lower in the test group(1.22％,2.44％,1.22％)than those in the control group(8.75％,10.00％,7.50％,P＜0.05).Conclusion The application of comfort nursing in patients with nasogastric feeding contributed to the enhancement of satisfaction degree of patients and nursing quality.

Objective To assess the predicting value of homocysteine ( HCY), low density lipoprotein cholesterin (LDL-C) and carotid intima-media thickness (IMT) for women with coronary artery disease (CAD). Methods To choose 115 patients with CAD and 102 patients of non-CAD;Homocysteine, LDL-C and IMT were assayed respectively;comparison and correlation analysis were performed based on genders. Results The rates for hyperhomocysteinemia and High Lipoproteinaemia in female CAD patients are 59. 6% and 75.0%,respectively,while they are 69. 9% and 78.6% for male CAD patients,which are both remarkably higher than 23.0% ,26.7%, 22. 2% and 23.6% in non-CAD patients ( Ps ＜ 0. 05 ). ROC curve shows that the best diagnostic boundary point is 0.953 mm for female IMT and 1. 021 mm for male IMT. At the boundary point,the sensitivity,specificity,positive predictive value and negative predictive value are 83.7% ,91.4% ,95.7% and 63. 8% in female, much higher than 62.9%, 65.3%, 76.2% and 53.3% in male. Conclusion hyperhomocysteinemia, high lipoproteinaemia are the important risk factors for women with coronary artery disease;IMT might be used as the predictor of CAD, which have more advantages for female than for male patients.

Mutations of BRCA1 and BRCA2 are closely related to developing of breast cancer andovarian cancer. There are a great diversity of founder mutations of BRCA1 and BRCA2. Their types and fre quency are greatly different in populations from different geographic regions and ethnicities. In particular ethnicgroups, founder mutations show a high frequency due to a founder effect. Compared with the expensive wholegenomic scan, screening of founder mutations which could provide information for the prevention and treatmentof related cancer is more convenient and easy to popularize with low cost.