Objection:To construct and characterize TNFR1-GFP fusion protein expression plasmid(pEGFP-TNFR1) and provide a direct and simplified method for assessment of the effect of TNFR1(siRNA) on the TNFR1 gene expression. Methods:Total RNA was extracted from mouse liver.TNFR1 cDNA was amplified by RT-PCR technique and cloned into pMD18-T vector and the sequence was ensured by sequenciog assay.TNFR1 gene was released from pMD-TNFR1 and subcloned into pEGFP-N2 upstream of GFP gene.pEGFP-TNFR1 was analyzed by restrictive enzymatic digestion to ensure the orientation.The plasmid was then transfected into CHO cells,then the fusion protein expression was detected by immunohistochemistry and fluorescent microscope.Rusults:A 1360 bp gene fragment was obtained and coloned into pMD18-T vector,and the sequence was correct.TNFR1 gene was subcloned into pEGFP-N2 vector,and then restriction endonucleases assays showed the correct orientation.24-48 hours after transfection in CHO cells,the expression of TNFR1-GFP fusion protein can be detected by immunohistochemistry and fluorescent microscope.Conclusion:pEGFP-TNFR1 has been constructed successfully.The TNFR1-GFP fusion proteinwas expressed in CHO cells after transtection.It may provide a direct and simplified method for primary assessment of the effect of TNFR1 siRNA on the(TNFR1) gene expression.

Objective: To improve the training system of medical response to nuclear and radiological emergency, and strengthen the capability construction of medical response staff. Methods: By drawing lessons from the training management experiences on medical response of nuclear and radiological emergency of the developed countries and combining with the situation and status quo of China, to discuss how to improve the training system construction on medical response of nuclear and radiological emergency under the guidance of relevant polices. Results:Through researching, a series of countermeasures, such as standard organization system, improving contents and methods of training, strengthening the construction of teacher team, carrying out the evaluation for training effect and intensifying the medical response awareness for the whole people, were adopted based on the existent main problems on medical response training of nuclear and radiological emergency in China. Conclusion: The medical response training of nuclear and radiological emergency is a long term work involved with strong specialty and wide knowledge range. This work need constant innovation so as to strengthen the training system construction of medical response, and train high competent talents on medical response of nuclear and radiological emergency, and then to establish a omnibearing, multilateral, scientific, systematic and long-acting training mode.

OBJECTIVE:To promote the effective development of hospital pharmaceutical care in China.METHODS:To analyse the disciplines closely related to hospital pharmaceutical care separately.RESULTS&CONCLUSION:Multidisci?plinary support is the basic feature of hospital pharmaceutical care.The cooperation among various technical persons is a deci?sive factor in effective development of hospital pharmaceutical care.

OBJECTIVE:To provide references for the improvement of membership system in Chinese drugstores.METHODS: Problems in the membership system were analyzed and concrete suggestions were put forward accordingly.RESULTS & CONCLUSION:The membership system of Chinese drugstores remains to be improved,and it needs to be deepened further in practice.

The distribution and morphological characteristics of catecholamine(CA)-containing neurons in the human brain stem of 7-9-month-old fetuses were studied by using Faglu histofluorescence method. The distribution of the main CA groups is consistent with the data provided by the literature concerning human infant and adult except for the presence of numerous small premature-looking fluorescent cells in the fetal area postrema which is lacking in the human infant and adult. As compared with most studies of other animals, the CA neurons of the dorsocaudal extension of the locus coeruleus in human appear to be more extensive and do not close to the ependyma of the fourth ventricle. Moreover, the CA neuronal population in human midbrain become more hypertrophic and appear to be more complexly organized in comparison with those in animals.

Objective To establish the baseline data about gross α and gross β radioactivity in local foods through a preliminary investigation in Urumqi City.Methods The edible parts of six types of foods, including cereals, vegetables, fruit, nuts, meat and dairy food, were pretreated and sampled.MPC 9604 low background o and gross β measuring instrument was used for determination of gross α and gross β radioactivity.Results The natural gross ot and gross β radioactivity in the cereal, vegetables, fruits, nut, meat, dairy food were 0.88-10.80 and 22.20-148.67 in cereals, 1.17-24.24 and 21.41-318.84 in vegetables, 1.44-4.89 and 43.90-112.67 in fruits, 17.49-43.72 and 287.81-747.40 in nut, 3.04 -19.77 and 41.55-164.51 in meat, and 2.07-2.94 and 44.07-59.02 Bq/kg in milk, respectively.Conclusions There are some differences in the gross natural radioactivity between Urumqi and the other provinces and cities due to possible regional differences.In order to further ascertain baseline of natural radioactivity in food in Urumqi, larger sample sizes and more diverse food types are needed for purposes of dynamic monitoring.

Fourty - seven cases were treated with this remedy, a~ mong them, 26 cases were markedly effective, 12 cases abated, 4 cases ineffective, the total effective rate being 91. 5%. The results were apparently superior to the blank control group. The mechanism of such effectiveness lies in the improvement of evacuation of gastric content and gastrointestinal movements.

Objective To assess the feasibility and the reliability of emergency temporary cardiac pacing under fluoroscopy and nofluoroscopy,and compare to the superiority and inferiority between two groups.Methods Fifty-seven patients were temporarily paced under fluoroscopy and nofluoroscopy by the way which the common bi-pole temporary endocardium pacing electrode was introduced into the right ventricle by the bi-subclavian vein and right internal jugular.Result Twenty-six patients were successfully paced under fluoroscopy,and 27 patients of 31 were successfully paced without fluoroscopy,the other 4 patients were not paced successfully.Besides,there was no any complication in all patients.Conclusion Emergency temporary cardiac pacing without fluoroscopy is utility,saving time and safety,Compared to other method,the effect is proximity and even superior than the way under fluoroscopy.The way of the emergency temporary cardiac pacing without fluoroscopy is applied widespreadly in the work of clinical first aid.

OBJECTIVE:To provide references on how to avoid price war in Chinese drug retailing.METHODS:The status quo of Chinese drug retailing was analyzed,meanwhile the cause and effect of price war were discussed.RESULTS & CONCLUSION:The disadvantages of price war outweigh the advantages,which is sure to be replaced by services-based brand strategy.

[Abstract ] Objective The role of serum Pygo2 expression in children with non-alcoholic fatty liver disease(NAFLD) in the disease process and whether it can be used to evaluate the degree of liver fibrosis still remain unknown .The article aimed to detect Pygo 2 expression in the peripheral blood of children with NAFLD and ana-lyze its relationship with traditional serum hepatic fibrosis index in or-der to evaluate the clinical significance of Pygo 2 measurement in chil-dren with NAFLD . Methods We enrolled 120 cases of childhood obesity and 18 healthy controls in Anhui Provincial Hospital and the First Affiliated Hospital of Nanjing Medical University from September 2014 to February 2016.The cases of childhood obesity were di-vided into simple obesity group ( n=44, no diffuse fatty liver under liver ultrasound detection ) , NAFL group ( n=35, diffuse fatty liver with normal liver function under liver ultrasound detection ) and NASH group ( n=41, diffuse fatty liver with abnormal liver function under liver ultrasound detection ) .The peripheral serum was collected from all patients and healthy controls .ELISA was used to detect serum Pygo2 expression and radioimmunoassay was used to detect the serum hyaluronia acid (HA), procollagen type Ⅲ(PCⅢ), pro-collagen type Ⅳ(CⅣ) and laminin(LN) levels.Finally the serum ALT and γ-GT levels were measured with totally automatic enzymat-ic method. Results Pygo2 expression in NAFL group [52.1(12.3)μg/L] and NASH group[78.3(50.0)μg/L] increased signifi-cantly compared with simple obesity group [43.2(18.7)μg/L](P<0.05).Pygo2 expression in NASH group increased significantly compared with control group [41.7(16.8)μg/L] and NAFL group (P<0.001).The serum hepatic fribrosis and inflammation markers ( HA, PC, ALT, C III and IV gamma-GT) levels gradually increased in the obese children .There were statistically positive correla-tions between serum Pygo2 and HA, PCⅢ, CⅣ, ALT or γ-GT (P<0.001).In particular, more significant positive correlations were found between serum Pygo2 and HA, CⅣor γ-GT (r=0.708, P<0.001;r=0.589, P<0.001;r=0.674, P<0.05). Conclusion The degree of liver fibrosis worsens with the increase of Pygo 2 expression and Pygo 2 is in remarkable correlation with conventional he-patic fibrosis serum makers ( HA, C IV) orγ-GT, which shows Pygo 2 expression can be taken as the clinical evaluation index of liver fibrosis in children with NAFLD .