Objective To determine the changes of serum glycosylphosphatidylinositol specific phospholipase D(GPI-PLD) activity in patients of several liver disease.Method Glycosylphosphatidylinositol (GPI) anchored placental alkaline phosphatase(PLAP) prepared by ourselves was used as a substrate.After partitioning by triton-X-114,the serum GPI-PLD activity was determined quantitatively and the data was treated by microware of SPSS 10.0.Results On the basis of the percentage of GPI-anchored PLAP conversion,the sera GPI-PLD activities of total 172 patients,included 26 severe acute viral hepatitis as group A,29 liver cirrhosis as group B,32 chronic viral hepatitis as group C,55 mild acute viral hepatitis as group D,30 primary hepatocellular carcinoma as group E,were measured.As compared with 182 healthy presons as control group,the sera GPI-PLD activities of group A and B were significantly reduced;By contraries,the activities of group D and E were significantly raised.The sera GPI-PLD activities of group C compared with healthy control group were not significantly altered.However,when paired Q-test,the changes of serum GPI-PLD activity between all paired groups among this five groups were remarkable.Conclusions The determination of sera GPI-PLD activities in patients can act as a biochemistry index for diagnosis of acute viral hepatitis,liver cirrhosis and primary hepatocellular carcinoma,as well as an auxiliary index for judgment of the curative effect and prognosis of liver diseases.

Objective To explore the correlation between the higher-order personality dimension(neuroticism) and the lower-order personality traits (alexithymia,dependence,self-criticism,perfectionism) in the sample of university students,and explore the effect of the higher-order personality dimension and lower-order personality traits to depression in the same sample.Methods A convenient sample of 563 university students from the two Universities College in Hunan province.These students were assessed with Center for Epidemiological Studies-Depression Scale (CES-D),Depressive Experiences Questionnaire (DEQ),The twenty-item Toronto Alexithymia Scales (TAS-20),Frost multidimensional perfectionism scale (FMPS) and neuroticism subscale in EPQ.Results (1) There were significant relationships between the total score of CES-D,each depressive symptoms and 10 personality factors,such as neuroticism,doubts about action and so on (The coefficients ranged from 0.105 to 0.569,P ＜0.05 or P＜0.01).(2)Factor analysis and multiple linear regression on the neurotic showed that doubts about action,concerned over mistakes,difficulties identifying feelings (DIF),difficulties describing feelings (DDF),dependency,self-criticism and neuroticism belonged to the factor 1 (the factor load coefficients ranged from 0.574 to 0.775).(3) Neuroticism can explained 32.3 ％ variance of depression (R 2adj =0.323),after 6 personality factors entered the regression equation,such as difficulties describing feelings (DDF),parental criticism and so on,the explained variance of depression increased to 43.2％ (R2adj =0.432).Conclusion There are overlapping and interaction between personality traits which include self-criticism,dependency,difficulties describing feelings(DDF),difficulties identifying feelings (DIF),concerned over the mistakes and neuroticism personality dimensions.The depressed affect are effectively predicted by neuroticism which is a effectively predict factor of depression,personality traits included self-criticism,dependency,alexithymia and malajustment perfectionism have a gain function of depression base on the Neuroticism.

OBJECTIVE: To discuss the distribution laws of traditional Chinese medicine (TCM) syndrome factor and their combination in coronary heart disease (CHD), and to study the correlation between the TCM syndrome factor combination and cardiac function as well as blood-lipid. METHODS: The parameters of the cardiac function of 300 patients with a final diagnosis of CHD by coronary angiography were measured by echocardiography, and the levels of blood lipids in the CHD patients were detected. An analysis of the correlation was done between the TCM syndrome factor combination and cardiac function as well as blood-lipid in CHD. RESULTS: The TCM syndrome factor combinations of CHD were blood stasis due to qi deficiency, qi and yin deficiency, intermingled phlegm and blood stasis, and yang deficiency and blood stasis. The ejection fraction of CHD patients with yang deficiency and blood stasis was markedly decreased. The levels of triglyceride and low-density lipoprotein cholesterol in CHD patients with intermingled phlegm and blood stasis were markedly increased, and the level of triglyceride in CHD patients with qi and yin deficiency was markedly increased too. CONCLUSION: The treatment of CHD should aim directly at the symptoms and causes. It is also proved that some compound traditional Chinese herbal medicines for supplementing qi and activating blood circulation, nourishing yin and resolving phlegm, and activating yang should be used in treatment of CHD. In cases of CHD with low cardiac function, particular emphasis should be laid on activating yang and blood circulation, while in cases of CHD with blood-lipid disturbance, particular emphasis should be laid on resolving phlegm and activating blood circulation, replenishing qi and nourishing yin.

Objective To undertake an updated genetic spectrum analysis in patients with hereditary spinocerebellar ataxia (SCA) in mainland China. Methods SCA 1, 2, 3, 6, 7, 8, 10, 12, 17 and dentatorubral-pallidoluysian atrophy (DRPLA) nucleotide repeat mutations were detected in 430 families with autosomal dominant SCA (ADCA) and 237 patients with sporadic ataxias by PCR and DNA sequencing. Subsequently, point and Indel (Insertion/deletion) mutation analyses of SCA5, SCA11, SCA13, SCA14, SCA15/16/29, SCA27, SCA31 and SCA35 were detected in 91 families with ADCA and 196 patients with sporadic ataxias excluded from SCA1, 2, 3, 6, 7, 8, 10, 12, 17 and DRPLA genotypes via PCR and Denaturing High Performance Liquid Chromatography (PCR-DHPLC), Multiplex ligation-dependent probe amplification and DNA direct sequencing analysis. Results Among the 430 ADCA families, there were 25 SCA1 (5.81%), 27 SCA2 (6.28%), 267 SCA3/MJD (62.09%), 8 SCA6 (1.86%), 8 SCA7 (1.86%), 1 SCA12 (0.23%), 1 SCA17 (0.23%) and 2 SCA35 (0.47%), and the remaining 91 families (21.16%) were genetically unidentified. Among the 237 sporadic SCA patients, there were 6 SCA1 (2.53%), 9 SCA2 (3.80%), 23 SCA3/MJD (9.70%) and 3 SCA6 (1.27%), and the remaining 196 (82.7%) were genetically unidentified. No pathogenic point mutation causing SCA5, SCA11, SCA13, SCA14, SCA27 or SCA31 subtypes was found. Conclusion SCA3/MJD is substantially the most common subtype in patients with ADCA and sporadic forms in mainland China, followed by SCA2, SCA1, SCA6 and SCA7. While SCA12, SCA17 and SCA35 are seldom found, SCA5, SCA8, SCA10, SCA11, SCA13, SCA27, SCA31 and DRPLA are very rare. The high proportion of genetically unidentified cases further verify that SCAs are of highly genetic heterogeneity, suggesting that other disease-causing genes might be involved in the negative ADCA pedigrees, and other etiological factors may involve in those sporadic cases other than genetics.

Objective:To investigate the diagnostic value of motility index (MI), time averaged mean velocity (TAMV) and blood lactic acid level for acute gastrointestinal injury(AGI) in critical patients.Methods:Patients were enrolled from January 2018 to June 2019 in Department of Emergency Intensive Care Unit, the People's Hospital of Guangxi Zhuang Autonomous Region. Patients were divided into the AGI group and control group according to whether there was acute gastrointestinal injury. Patients’ general information and AGI characteristics were assessed. Area under the receiver operating characteristic (ROC)curve was used to analyze the predictive value of MI, blood lactic acid level and TAMV, or combination on the occurrence of AGI.Results:A total of 73 critical patients were enrolled including 45 patients with AGI and 28 without. Logistic regression analysis found that MI ( OR=2.618, 95% CI: 1.214-5.646, P=0.014), TAMV( OR=1.483, 95% CI: 1.058-2.077, P=0.022), blood lactic acid level( OR=0.360, 95% CI: 0.002-0.865, P=0.040) at admission were independent risk factors for AGI. The sensitivity and specificity of MI, blood lactic acid level and TAMV in predicting AGl were 100% and 89.3%, respectively (AUC=0.982, Youden index=0.893). High blood lactic acid level and low MI and TAMV are independent risk factors for the development of AGI in critical patients. The predictive cut-off values are 4.44 for MI, 45.79 cm/s for TAMV and 5.03 mmol/L for blood lactic acid level. Conclusions:Combination of MI, TAMV and blood lactic acid level has apractical predictive value for AGI incriticalpatients

OBJECTIVETo evaluate the effect of core ceramic surface treatment on the failure behaviors of two bilayered all- ceramic structures.

METHODSLithium disilicate glass-ceramic (LDG, IPS e.max Press) and Zirconia(Cercon) specimens were divided into 4 groups(n=12) according to the following surface treatment methods: ground successively to 1 000(group A), 600(group B) and 320(group C) grit silicon carbide(SiC) papers and sandblasted with 110 µm aluminum oxide particle(group D, as control). Surface roughness was measured using a profilometer. After veneer sintering, the flexural strength σ was measured with biaxial flexure test and the reliability of strength was analyzed with Weibull distribution. Failure modes and fractographic patterns were analyzed by optical stereo and scanning electron microscopy (SEM).

RESULTSFor LDG, no significant difference was found for the flexural strength values among groups (F=0.406, P=0.750). No significant difference was found in Weibull modulus among groups(the confidence bounds overlapped with each other). SEM and optical stereo observation results showed that all the bilayered LDG specimens failed cohesively. For zirconia, Mean flexural strength values were (541 ± 75), (533 ± 73), (529 ± 78) and (640 ± 95) MPa for groups A, B, C and D, respectively, and group D demonstrated a significantly higher flexural strength compared to the other three groups(P<0.05). No significant difference were found among groups(the confidence bounds overlapped with each other). Specimens in all groups failed mainly adhesively, while those in group D presented lower percentage of adhesive failure.

CONCLUSIONSCore ceramic surface treatment has no influence on the failure behaviors of LDG based bilayered structures. Sandblasting can increase the flexural strength of bilayered zirconia structures by reducing the incidence of delamination.

Aluminum Oxide ; Ceramics ; chemistry ; Dental Porcelain ; chemistry ; Dental Stress Analysis ; methods ; Humans ; Pliability ; Reproducibility of Results ; Surface Properties ; Zirconium ; chemistry

Objective:To analyze the molecular epidemiological characteristics of the Corona virus disease 2019 (COVID-19) cases in Shijiazhuang, which can reveal the origin of the outbreak and provide a scientific basis for COVID-19 prevention and control.Methods:From January 2 to January 8, 2021, a total of 404 samples from 170 COVID-19 cases were collected from the Shijiazhuang Fifth Hospital. The consensus sequence of 2019 novel Coronavirus(2019-nCoV) was obtained through multiplex polymerase chain reaction-based sequencing. The sequences of 170 COVID-19 cases were analyzed by the PANGOLIN, and the data were statistically analyzed by T-test.Results:Among the 404 COVID-19 samples, a total of 356 samples obtained high quality genome sequences (>95%,100×sequencing depth). The whole genome sequences of 170 COVID-19 cases were obtained by eliminating repeated samples. All 170 sequences were recognized as lineage B1.1 using PANGOLIN. The number of single nucleotide polymorphism arrange from 18-22 and most of the single nucleotide polymorphism were synonymous variants. All of 170 genomes could be classified into 48 sub-groups and most of the genomes were classified into 2 sub-groups (66 and 31, respectively).Conclusions:All cases in this study are likely originated from one imported case. The viruses have spread in the community for a long time and have mutated during the community transmission.