miRNA ( microRNA) is a small molecule, which is composed of about 20 nucleotides, and has been a hot research topic in recent years.Many studies indicated that the expression level of miRNA in tumor changed, which played a role in the differentiation and proliferation of tumor cells, as a function of oncogene or tumor suppressor gene.The occurrence and development of colon cancer is related to the abnormal expression of many genes, and the data shows that the abnormal expression of miRNA is closely related to the development of colon cancer.In this paper, the influence of miRNAs on the development of colon cancer and its relevant mechanism were collected, then the colon cancer occurrence, development and miRNA molecular mechanisms were studied, which provided a theoretical basis for the molecular target treatment of colon cancer.

Objective To discuss diagnosis,treatment and late effects of three patients with acute radiation-induced skin injury after an accident.Methods Medical history collection and physical examination were made in a manner of one or several doctors to one patient.The general health condition was evaluated through laboratory studies,including complete blood count,liver and kidney function,thyroid function and humoral immunity measurement.The genetic analysis of radiation damage was performed by using chromosomal aberrations and micronucleus assay (CB method).The psychological status of the patients was evaluated during medical follow-up.Results Among the three patients,two were diagnosed with degree Ⅲ acute radiation-induced skin injury and one with degree Ⅱ.The medical follow-up showed that two patients had syndrome of neurasthenia.The two cases with degree Ⅲ acute radiation-induced skin injury then became chronic radiation-induced skin damage.Conclusions Acute radiation-induced skin injury could be persistent and become chronic radiation skin damage.It is important for long-term medical follow-up to be taken for patients with acute radiation-induced skin injury.

Objective: To observe operative results of tibiotalocalcaneal arthrodesis (TTC) via lateral transfibular ap-proach in the treatment of severe ankle and subtalar diseases. Methods: Eighteen patients treated with TTC via lateral transfibu-lar approach from January 2015 to December 2017 were retrospectively analyzed. There were 11 males and 7 females from 39 to 76 years old, with an average age of 53.4 years. Preoperative diagnoses included traumatic arthritis (3 cases), osteoarthritis (10 cas-es), talar necrosis (3 cases), failure of ankle fusion (1 case) and Charcot-Marie-Tooth disease (1 case). Ankle and hindfoot deformi-ty included varus (10 cases), valgus (6 cases), equinus (1 case) and equinovarus (1 case). All cases were classified into stage-3 ar-thritis according to Morrey-Wiedeman classification. All patients sustained pain and stiffness in the ankle and hindfoot as well as walking dysfunction. Preoperative and 1 year postoperative visual analogue scale (VAS) and American Orthopaedic Foot and An-kle Society (AOFAS) ankle-hindfoot scale were collected. Postoperative joint fusion and complications were observed. Results: Follow-up ranged from 12-47 months, averaging 25.4 months. Preoperative VAS scores were 6-9, averaging 7.1±1.0, while postop-erative VAS scores at 1 year follow-up were 0-2, averaging 0.9±0.8, which decreased significantly. Preoperative AOFAS scores were 24-59, averaging 40.6±11.5, while postoperative AOFAS scores at 1 year follow-up were 68-84, averaging 75.3±5.8, which increased significantly. 8 cases were good and 10 cases were fair according to AOFAS scores at 1 year follow-up. The excellent and good rate was 44.4% (8/18). All joints were fused successfully without implants loosening or breakage. All patients satisfied with operative results at 1 year follow-up. Lateral distal wound dehiscence happened in 1 case of traumatic arthritis and the wound was finally healed after debridement and vacuum assisted closure (VAC). Two cases of osteoarthritis presented with dorsal lateral foot numbness and became asymptomatic after neurotrophic medication. One case of osteoarthritis complained of discomfort in the lateral heel caused by implants prominence and implants were removed at 1 year after surgery. Postoperative complication rate was 22.2% (4/18) . Conclusion TTC via lateral transfibular approach with cannulated screws and locking plate system is safe and ef-fective. Joint fusion rate is high and complication rate is low. During operation, attention should be paid to restoring hindfoot align-ment, soft tissue balance, proper bone graft and joint compression.

Objective To observe operative outcomes of suture-button technique for acute Lisfranc injury.Methods A retrospective observation was conducted of the 11 patients who had been operatively treated with suture-button technique and plate fixation for acute Lisfranc injury from January 2015 to December 2016.They were 8 men and 3 women,aged from 22 to 54 years(mean,32.1 years).By the Myerson classification for the injury,there were 9 cases of type B2 and 2 cases of type Cl;by the Chiodo classification,there were 7 cases of middle column injury,2 cases of injury to middle and lateral columns and 2 cases of injury to medial and middle columns.Their scores on the visual analogue scale(VAS),mid-foot scores of American Orthopedic Foot and Ankle Society(AOFAS),bone union and complications were observed post-operatively.Results Follow-ups for this cohort lasted for 14 to 34 months(mean,20.5 months).Follow-ups revealed that their VAS scores for the affected foot averaged 0.6(from 0 to 2),significantly higher than those for the nomal foot(Z=2.070,P=0.038).Their AOFAS scores averaged 95.2(from 87 to 100),significantly lowerer than those for the nomal foot(Z=2.121,P=0.034).All fractures were united well.Conclusion Suture-button technique can lead to satisfactory outcomes for acute Lisfranc injury.

【Objective】 To investigate the effect of autologous blood transfusion(ABT) drainage system and simple drainage(using drainage bags) on the prognosis of patients after lumbar surgery. 【Methods】 The patients admitted to the Department of Orthopedics of our hospital from August 2018 to September 2020 who underwent posterior open lumbar internal fixation and fusion were divided into two groups according to different drainage methods adopted after surgery: 50 patients were randomly selected from the patients who received postoperative ABT system for drainage as ABT group, and 50 patients were randomly selected from the patients who received postoperative drainage by drainage bag as simple drainage bgroup(the control group). The postoperative drainage volume, actual postoperative drainage, total dominant blood loss, total autologous blood transfusion volume, as well as the postoperative anemia indexes, infection indexes and albumin levels in d1, d3 and d7 of the 2 groups were retrospectively analyzed. 【Results】 The gender, age, operation duration and operation segment of the 2 groups were comparable (P>0.05), and preoperative Hb, Hct, ALB, WBC, NE%, intraoperative blood loss, intraoperative autologous blood transfusion volume and actual postoperative drainage volume were similar (P>0.05). There were no significant differences in Hb, Hct, ALB, WBC, NE% and CRP in postoperative d1, d3 and d7, as well as in preoperative and postoperative Hb, Hct and ALB, and in postoperative and preoperative WBC, NE% and CRP (P>0.05). The postoperative drainage volume (mL) and total dominant blood loss (mL) in ABT group and the control group were 554.40±176.82 vs 337.80±102.43, and 1 048.40±282.87 vs 791.80±277.02, respectively (P<0.05). 【Conclusion】 The use of ABT drainage system after lumbar surgery increased the drainage volume compared with simple drainage, but the improvement of anemia, albumin and infection was not obvious. ABT system should not be used routinely for drainage after lumbar surgery.