1.Application of vaseline gauze and cotton piece in eye protection during otological surgery
Yushuang WANG ; Min ZHUANG ; Jinhua YU
Modern Clinical Nursing 2015;(8):23-25
Objective To explore the effects of vaseline gauze combined with brain cotton piece on eye protection during otological surgery . Methods From January to March in 2015 , 80 patients with otological surgery were set as the experiment group , where vaseline gauze and brain cotton piece were used to protect eyes . From October 2014 to December 2014 , another 80 patients with otological surgery were set as the control group , where aureomycin eye ointment was used for eye protection . The two groups were compared in terms of complications incidence . Result The complication incidence of the experiment group was significantly lower than that of control group ( P < 0 . 01 ) . Conclusion Vaseline gauze and brain cotton piece can effectively protect the eyes for patients undergoing otological surgery , lower the occurrence rate of fewer complications and improve patients′comfort .
2.A research about clinical effects of using different intervention methods to ameliorate the pains of neonatal infants
Yaping SHI ; Jiangqin LIU ; Jianguang WANG ; Zhenliang LIN ; Yushuang JIA
Chinese Journal of Practical Nursing 2006;0(19):-
Objective Compare the analgesia effects of using different intervention method among neonatal infants, and then find out the most effective method. Methods Divided 120 neonatal infants into the control group, the NNS group and the position group, there were 40 cases in every group. Using the N-PASS scale evaluated the pain degree at the points of 1 and 5 minutes respectively after stimulation among the 3 groups. Results There was significant difference between the 3 groups on the pain degree,P
3.Study on General Reproductive Toxicity of Oral Administration of Pineapple Leaves Extract in Mice
Zhiyi YUAN ; Xi LU ; Fan LEI ; Yushuang CHAI ; Tianshi FENG ; Jingfei JIANG ; Shuang ZHAO ; Xinpei WANG ; Huiyu LI ; Dongming XING ; Lijun DU
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(5):966-971
This article was aimed to study the general reproductive toxicity in mice in order to give a better evalua-tion of the medicinal plant of pineapple leaves (A nanas comasus L). Adult male and female mice were orally admin-istered with pineapple leaves. And then, each of the male and female mice was put together in one cage for mating. The mating success females were fed continuously. The experimental observation was conducted in pregnancy, fetal development, as well as the offspring of mice. The results showed that in addition to a large dose of pineapple leaves (4 g·kg-1) of the parental male rats having a lower body weight, pineapple leaves did not significantly affect on other parameters. There were no significant effects on pregnant mice and their offspring of mice. It was concluded that the pineapple leaves did not influence the general reproductive function of mice apparently.
4.Conditions for protoplast preparation of spinosyn-producing strain and the physiological properties of protoplast-regenerated strains.
Yushuang LUO ; Xuezhi DING ; Liqiu XIA ; Hailong WANG ; Fan HUANG ; Ying TANG
Chinese Journal of Biotechnology 2009;25(3):360-367
To improve spinosyn-producing strain and enhance spinosyns yield, we studied the effects of glycin concentration and the operational time, temperature and lysozyme concentration on protoplast preparation of Saccharopolyspora spinosa SP06081. We also studied different regeneration media and osmotic stabilizing agents. In addition, we compared the change of morphology and spinosyns yield of the regenerated strains. The results showed that the Saccharopolyspora spinosa SP06081 protoplast yield was the highest under these conditions: the collected mycelium from SP06081 grown in Tryptic Soy Broth (TSB) medium with 0.2% glycin for 48 h was treated by 0.1 mg/mL lysozyme at 28 degrees C for 20 min, then plated on the R2YE medium with sucrose as osmotic stabilizer, the number of regeneration protoplast was up to 10(8)/mL. The protoplast-regenerated strains exhibited changes in morphology and antibiotic production, 29.3% protoplast-regenerated strains was characterized by loose mycelium and abundant broken branches as did their parent. Among them, 58.2% strains presented the trend to positive variation in spinosad yield, with the highest spinosad yield of up to 582.0 mg/L, 85.6% higher than that of their parent. There is significant correlation between the morphological differentiation and antibiotic yield of the protoplast-regenerated strains from spinosyn-producing strain.
Culture Media
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pharmacology
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Drug Combinations
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Glycine
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pharmacology
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Insecticides
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metabolism
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Macrolides
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metabolism
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Muramidase
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pharmacology
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Protoplasts
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cytology
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drug effects
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Regeneration
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Saccharopolyspora
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genetics
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metabolism
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physiology
5.Recent advance in epoxyeicosatrienoic acids in intracerebral hemorrhage
Hongmin WEI ; Jixu YU ; Zhenchuan LIU ; Yushuang WANG ; Huimin ZHU ; Fengyuan CHE
Chinese Journal of Neuromedicine 2020;19(1):93-97
Epoxyeicosatrienoic acids (EETs) are mainly from intracellular arachidonic acids catalyzed by cytochrome P450 cyctooxygenase and degraded to lower active dihydroxyeicosapentaenoic acids by soluble epoxide hydrolase.In recent years,EETs have been found to be a new target for prevention and treatment of various nervous system diseases,such as anti-inflammatory reaction,anti-atherosclerosis,anti-cell apoptosis and angiogenesis.Intracerebral hemorrhage is a kind of serious acute cerebrovascular disease.Cerebral hemorrhage is a kind of acute cerebrovascular disease;secondary injury is one of the important mechanisms of cerebral hemorrhage;the present studies have confirmed that EETs have protective role in brain tissues after cerebral hemorrhage,thus,become new hotspot in the research of cerebral hemorrhage.This review focuses on the role and mechanism of EETs in intracerebral hemorrhage,hoping to provide some references for exploration of new research directions and therapeutic targets in the treatment ofintracerebral hemorrhage.
6.Effects of Atractylenolide Ⅰ,Atractylenolide Ⅱ and Atractylenolide Ⅲ on Cytokines Expression of Inflam-matory Macrophages
Qinhua CHEN ; Fei YU ; Hongmei WANG ; Xueru DING ; Jun ZHU ; Yushuang LIU ; Lun ZHANG
China Pharmacist 2017;20(12):2112-2116
Objective:To study the effect of atractylodes lactideⅠ,Ⅱand Ⅲ on the expression of cytokines by inducing M1 type macrophage model. Methods:Apoptotic macrophages were induced by sodium thioglycolate, and then the rat peritoneal inflammatory macrophages were purified by a differential adherence method. The expression of macrophage marker antigen (ED1) was identified by flow cytometry macrophages. Tumor necrosis factor ( TNF-α) , proinflammatory cytokines ( IL-1β) and interleukin-6 ( IL-6 ) were measured by ELISA in vitro. The levels of expression of arginase 1, inducible nitric oxide synthase (iNOS), inflammatory cytokines (IL-1β) chemokine (CCL22) and transforming growth factor (TGF-β) in inflammatory macrophages were determined by RT-PCR. Results:As for the purification of cultured rat inflammatory macrophage expressing ED1, atractylenolideⅠand Ⅲ could reduce the ex-pression levels of iNOS and IL-1βand increase the expression levels of arginase1 CCL22 and TGF-β. The release of inflammatory fac-tor IL-1β decreased, and the release levels of TGF-β and chemokines CCL22 were promoted(P<0. 05). Atropine lactoneⅠ and Ⅲ had significant inhibitory effects on TNF-α, IL-1β and IL-6 in macrophages(P<0. 05). Conclusion: Atractylodes lactone Ⅰand Ⅲ with anti-inflammatory activity can promote the expression of cytokines in inflammatory macrophages, while the change induced by at-ractylaxanthin Ⅱ is not obvious.
7.The investigation on the status and associated factors of uncertainty of caregivers of children with imperforate anus
Hongzhen XU ; Dan WANG ; Shuohui CHEN ; Yushuang JIA ; Wei GAO
Chinese Journal of Practical Nursing 2020;36(18):1405-1410
Objective:To investigate the uncertainty level of caregivers of children with imperforate anus, and determine the influence factors.Methods:Two hundred and twenty-nine caregivers were enrolled from three tertiary children′s hospital in Eastern China. The caregivers completed the Perception of Uncertainty Scale, Parent Stigma Scale and Social Support Scale. The demographic information of caregivers and affected children were also collected during the survey.Results:The average score of uncertainty of caregivers was (72.58±14.06) points, and multiple analysis showed that education level, the acceptance level of disease, social support and stigma were independent determinants of uncertainty ( P<0.01), and these factors could account for 26.9% of the variance. Conclusions:The caregivers of children with imperforate anus experience a high level of uncertainty, especially those with low education level, share low acceptance level of disease, receive low social support and experience higher level of stigma.
8.Study on the immunization status and its influencing factors among workers from the polio network laboratories in China
Yushuang GUO ; Jiaqi WANG ; Chao XU ; Yaning LIU ; Xiaohui HE ; Qiang WEI
Chinese Journal of Epidemiology 2017;38(6):737-739
Objective To Investigate the immune status and influencing factors of provincial polio network laboratory (PNL) workers in China so as to provide evidence for the development of related strategies to protect personnel working at the PNLs.Methods All the practitioners from the PNLs at the provincial centers for disease control,were selected as objects for this study,from October to December,2016,under a questionnaire survey.Information on status of immunity and influencing factors was collected,with SAS software,trend chi-square used for statistics analysis.Results A total of 77 workers were involved in this survey,with 60 (78%) of them completed the polio-based immune program but the rest 17 (22%) remained records unclear.66 people (about 86%) remembered clearly that they had received vaccination when engaging in the polio-lab work,but the rest 11 (14%) with only partial vaccination records.We also noticed that the Influencing factors realted to vaccination status were:age (x2 =2.48,P<0.05),title (x2 =2.51,P<0.05),years of employment (P<0.000 1),education (x2 =0.74,P=0.46) and gender (x2 =0.46,P=0.50).Conclusion Immune status of the Chinese provincial PNL practitioners appeared fairly good as 86% of all the workers had received polio-related vaccination,with 41% of them completed a 3-time inoculation program,when started working in this field.
9. Mucopolysaccharide polysulfate cream for reducing the occurrence of eczema and skin atrophy in patients with moderate- or low-risk infantile hemangioma after treatment: a multicenter clinical study
Li LI ; Hua WANG ; Liuhui WANG ; Yanping GUO ; Qiufang QIAN ; Lu YU ; Wei SONG ; Rui XU ; Xiao LIN ; Chunhua TAN ; Yushuang HAO ; Lin MA
Chinese Journal of Dermatology 2019;52(10):779-784
Objective:
To investigate whether topical mucopolysaccharide polysulfate (MPS) cream can reduce the incidence of eczema and skin atrophy in patients with moderate- or low-risk infantile hemangioma after the treatment with topical beta-blockers or 595-nm pulsed dye laser (PDL) , and to analyze factors influencing the occurrence of eczema and skin atrophy.
Methods:
A total of 722 patients aged 0- 1 years with moderate- or low-risk infantile hemangioma were enrolled from 5 Children′s Hospitals in China. According to the disease condition and therapy acceptability, these patients were divided into 6 groups to be treated with topical beta-blockers and MPS cream (blocker+ MPS group) , topical beta-blockers (blocker group) , 595-nm PDL and topical MPS cream (PDL+ MPS group) , 595-nm PDL (PDL group) , 595-nm PDL combined with topical beta-blockers and MPS cream (PDL+ blocker+ MPS group) , and 595-nm PDL and topical beta-blockers (PDL+ blocker group) , respectively. All the externally applied agents were applied twice a day, and PDL was performed once every 4 weeks. Efficacy and adverse reactions were evaluated after 3-month treatment. Univariate and multivariate Logistic regression analyses were carried out to analyze factors influencing the incidence of eczema and skin atrophy in patients with infantile hemangioma after treatment, and chi-square test was carried out to compare efficacy among the groups.
Results:
After 3-month treatment, multivariate Logistic regression analysis for comparing the blocker+ MPS group with blocker group showed that the risk factor for eczema on the surface of hemangiomas was no topical treatment with MPS cream (
10. Consistency of ALK Ventana-D5F3 immunohistochemistry interpretation in lung adenocarcinoma among Chinese histopathologists
Lin LI ; Liping ZHANG ; Yuchen HAN ; Weiya WANG ; Yan JIN ; Qingxin XIA ; Yueping LIU ; Jin XIANG ; Chao LIU ; Shanshan LU ; Wei WU ; Zhen CHEN ; Juan PANG ; Yanfeng XI ; Yushuang ZHENG ; Dongmei GU ; Jun FAN ; Xiaona CHANG ; Weiwei WANG ; Liang WANG ; Zhihong ZHANG ; Xiaochu YAN ; Yi SUN ; Ji LI ; Feng HOU ; Jingyuan ZHANG ; Rongfang HUANG ; Jianping LU ; Zheng WANG ; Yongbin HU ; Hongtu YUAN ; Yujie DONG ; Lu WANG ; Zhenyu KE ; Jingshu GENG ; Lei GUO ; Jing ZHANG ; Jianming YING
Chinese Journal of Pathology 2019;48(12):921-927
Objective:
To understand the consistency of ALK Ventana-D5F3 immunohistochemistry (IHC) interpretation in Chinese lung adenocarcinoma among histopathologists from different hospitals, and to recommend solution for the problems found during the interpretation of ALK IHC in real world, with the aim of the precise selection of patients who can benefit from ALK targeted therapy.
Methods:
This was a multicenter and retrospective study. A total of 109 lung adenocarcinoma cases with ALK Ventana-D5F3 IHC staining were collected from 31 lung cancer centers in RATICAL research group from January to June in 2018. All cases were scanned into digital imaging with Ventana iSCANcoreo Digital Slide Scanning System and scored by 31 histopathologists from different centers according to ALK binary (positive or negative) interpretation based on its manufacturer′s protocol. The cases with high inconsistency rate were further analyzed using FISH/RT-PCR/NGS.
Results:
There were 49 ALK positive cases and 60 ALK negative cases, confirmed by re-evaluation by the specialist panel. Two cases (No. 2302 and No.2701) scored as positive by local hospitals were rescored as negative, and were confirmed to be negative by RT-PCR/FISH/NGS. The false interpretation rate of these two cases was 58.1% (18/31) and 48.4% (15/31), respectively. Six out of 31 (19.4%) pathologists got 100% accuracy. The minimum consistency between every two pathologists was 75.8%.At least one pathologist gave negative judgement (false negative) or positive judgement (false positive) in the 49 positive or 60 negative cases, accounted for 26.5% (13/49), 41.7% (25/60), respectively, with at least one uncertainty interpretation accounted for 31.2% (34/109).
Conclusion
There are certain heterogeneities and misclassifications in the real world interpretation of ALK-D5F3 IHC test, which need to be guided by the oncoming expert consensus based on the real world data.