Objective To investigate the effect of intestinal flora on the efficacy of polyethylene glycol losenatide(PEX168)in mice with type 2 diabetes mellitus(T2DM).Methods A total of 50 healthy male C57BL/6 mice were fed with a high-fat diet combined with STZ to establish a T2DM mouse model.Among them,40 were successfully modeled and divided into T2DM group(n=10)and PEX168 group(n=30).PEX168 group was further divided into two sub groups according to HbA1c:ideal group(IE subgroup,HbA1c≤6.5%,n=12)and unsatisfactory group(NE subgroup,HbA1c>6.5%,n=12).IE subgroup was fecal donor,NE subgroup was recipientand further divided into fecal bacteria transplantation subgroup(FMT,n=5)and Sham subgroup(Sham,n=5).Fecal bacteria transplantation(FMT)was used to transfer fecal bacteria from IE group to FMT group.Body weight,food intake and blood glucose were measured every 2 weeks in all the groups.FPG,FIns,HbA1c and insulin resistance index(HOMA-IR)were compared on the 7th day after 10 weeks of intervention in all the groups.Results FPG and body weight were lower at the 4th,6th,8th and 10th week(P<0.05),and food intake was lower in PEX168 group than in T2DM group at the 2nd,4th,6th,8th and 10th week(P<0.05).At the 4th,6th,8th and 10th week after administration,the FPG and body weight were lower in IE subgroup than in NE subgroup(P<0.05),and the food intake was lower at the 0th,4th,8th and 10th week after administration than in NE subgroup(P<0.05).The FBG,body weight and food intake were lower in FMT subgroup than in Sham subgroup at 4,6 and 8 weeks(P<0.05).The FPG,HbA1c,2 hPG,FIns and HOMA-IR were lowerin PEX168 group after treatment than in PEX168 group before treatment and T2DM group after treatment(P<0.05).FPG,HbA1c,FIns,2 hPG and HOMA-IR were lower in IE subgroup after treatment than in IE subgroup before treatment and NE subgroup after treatment(P<0.05).FPG,HbA1c,FIns,2 hPG and HOMA-IR were lower in FMT subgroup after treatment than in FMT subgroup before treatment and Sham subgroup after treatment(P<0.05).Conclusions Differences in intestinal flora between individuals affect the efficacy of PEX168.FMT treatment can improve the composition of intestinal flora and affect the efficacy of PEX168.

Pemphigus is a group of life-threatening chronic autoimmune bullous diseases. It is currently believed that the humoral immunity mediated by anti-desmoglein IgG antibodies is the primary mechanism underlying the occurrence of pemphigus. However, recent studies have indicated a critical role of various T cell subsets in the occurrence of pemphigus.

Objective To analyze the medication law and academic thoughts of Professor Wang Junhong in the treatment of tic disorders(TD)in children.Methods The cases of children with TD diagnosed and treated by Professor Wang Junhong from January 2015 to November 2022 were selected.Excel 2016 was used to analyze the clinical information of children with TD.The frequency ranking,property,taste and meridian tropism,and changes of prescription drugs were analyzed in multiple dimensions.SPSS Modeler 18.0 was used to analyze the drug association rules of prescriptions in 2021 and 2022.Cytoscape 3.9.0 was used to analyze the complex network of drug-drug strong,medium and weak links obtained by SPSS Modeler 18.0.The drug groups were obtained in SPSS,and Excel 2016 was used to analyze the annual changes of high-frequency drugs.Results Totally 5586 prescriptions were included,involving 198 kinds of Chinese materia medica,with a total frequency of 108356 times.The top five kinds of high-frequency Chinese materia medica were Chrysanthemi Flos,Acori Tatarinowii Rhizoma,Coptidis Rhizoma,Crataegi Fructus,Polygalae Radix.The medicinal properties were mostly cold,warm and mild.The medicinal tastes were mainly bitter,sweet and pungent.The main meridians of drugs were liver,heart and lung meridians.The association rule analysis showed that the common couplet medicines were Chrysanthemi Flos-Acori Tatarinowii Rhizoma and Acori Tatarinowii Rhizoma-Scorpio.Commonly used triple combination was Chrysanthemi Flos-Scorpio-Acori Tatarinowii Rhizoma.Clustering analysis showed 4 drug groups,reflecting the characteristics of Professor Wang Junhong's treatment of calming liver and tranquilizing mind.According to the time-flow analysis,since 2020,the proportion of drugs such as Bupleuri Radix,Scutellariae Radix,Haliotidos Concha,Gastrodiae Rhizoma and Margaritifera Concha have gradually increased,indicating that more attention should be paid to treating the liver,resolving phlegm and calming the mind.Conclusion In the treatment of TD in children,Professor Wang Junhong takes heart,liver and lung as the center.The prescription medication is to relieve wind and phlegm,soothe the liver and tranquilize the mind.In recent years,it has attached importance to the role of regulating emotions and resolving phlegm in the treatment of children with TD.

ObjectiveTo investigate the regulatory effect of Changju Zhidong prescription on gut microbiota structure of children with tic disorder（TD）. MethodTwenty-four children with TD who visited the pediatric outpatient clinic of Dongzhimen Hospital of Bejjing University of Chinese Medicine from November 2020 to January 2022 were selected as the observation group， and eight healthy children were selected as the normal group. The observation group was treated with Changju Zhidong prescription for 12 weeks， and the clinical efficacy was observed by Yale global tic severity scale（YGTSS）， traditional Chinese medicine（TCM） syndrome score， and score of TCM symptom evaluation scale for pediatric gastrointestinal tract. Meanwhile， The fecal samples of the observation and normal groups were collected before and after treatment， and 16S rDNA sequencing was used to explore the effects of Changju Zhidong prescription on the distribution of gut microbiota of the children. ResultCompared with the pre-treatment， the YGTSS， TCM syndrome score， and score of TCM symptom evaluation scale for pediatric gastrointestinal tract of the observation group were significantly reduced after 12 weeks of treatment（P<0.05）， Simpson and Pieloue indices in alpha diversity were significantly reduced（P<0.05）. Before treatment， the relative abundance of Streptococcus parasanguini in the observation group was significantly higher than that of the normal group， while the relative abundances of Citrobacter， Lactobacillus， Lacticaseibacillus， Muribaculaceae unclassified， Odoribacter and 7 other bacterial groups were significantly lower than that in the normal group. Compared with the pre-treatment， the observation group showed a significant decrease in Ruminococcus torques group unclassified and Ruminococcus torques group， and a significant increase in Olsenella， Acetivibrio and 4 other bacterial groups. Functional enrichment analysis showed that the main pathways of Changju Zhidong prescription regulating gut microbiota were bacterial secretion system， insect hormone biosynthesis， limonene and pinene degradation， novobiocin biosynthesis， tropane piperidine and pyridine alkaloid biosynthesis. ConclusionThe pathogenesis of children with TD is accompanied by intestinal flora disorders， and Changju Zhidong prescription can improve core and gastrointestinal symptoms， increase the abundance of beneficial and decrease the abundance of harmful gut microbiota in children with TD， and its mechanism may be through the metabolism of terpenoids and polyketides.