AIM: To establish a method for determination of schizandrol A in Mian'er'an Dripping Pills (Semen Ziziphi Spinosae, Fructus Schisandrae, etc.). METHODS: HPLC was used with hypersil C 18 column (250mm?4.6mm; 5?m), methanol water (65∶35) as a mobile phase and detection wavelength at 254nm. The flow rate was 1.0mL?min -1 and at room temperature. RESULTS: The calibration curve was linear (r= 0.9999). The average recovery was 100.2% and RSD was 2.9%, respectively. CONCLUSION: The method is simple, quick and specific, and provides a quality control for the dripping pills.

ObjectiveTo investigate the protection of extract form earthworm a traditional Chinese medicine in auricle microcirculation disturbance in mice. MethodThe microcirculation disturbance in mice was brought by rapid injection of high molecule dextran. Observe the microvascular diameter,the blood flow speed,the blood fluid state and crossing net patency quantity of micrangium in mice before injection and after injection 10min,20min,30min with WX-9 microcirculation microscope and analytical system. ResultHigh and low dose of extract form earthworm could expend the diameter of the vein and artery of micrangium, quicken the blood flow speed,improve the blood fluid state,increase micrangium crossing net patefaction numbers. To compare with the control group, threr’s significant difference (P

Lappaconitine (LA) 0. 5 mg? kg-1had significant antagonistic action on the ar-rythmia induced by 3-acetylaconitine ( AA ) 0.07 mg?kg-1in rat. When LA and AA were used in combination (7:1) in mice, the analgesic activity of AA was not influenced marked-ly by LA but the LD50 of AA was increased by 50% ,therefore the therapeutic index of AA was increased and the margin of safety was widened.

Objective:To investigate the pain in patients during the early stages of recovery from maxillofacial surgery under general anaesthesia.Methods: One hundred patients as participants to respone the questionaire of postoperative pain 24 hours after operation. The data of pain intensity,analgesia requirement and the reason for operation of the patients were collected and analyzed. Results: Among the 100 cases, 92 with moderate or slight postoperative pain, 8 with severe pain. It is significant more severe pain (14.3%)in the group of bone fracture than that (less than 8.7%) in other groups. Conclusion: Most patients 24 h after maxillofacial surgery are with moderate and slight pain. The pain intensity relates with type of maxillofacial surgery.

Comparative medicine is a synthetical subject,to provide protection and improve healthy medicare of human being by studying all kinds of human diseases using laboratory animals.Comparative medicine is widely involved in training medical talents,elucidating disease mechanism,improving medicare level and benefiting human healthy services.Therefore,comparative medicine research is an important foundation of medicine development,and indispensable supporting condition and organic compartment in all-round development of "medication,education and research" in modern comprehensive hospital.

The construction of the model curricula is the important branch of teaching quality engineering. The key to the development of the model curricula lies in the reform of the teaching model. As required to the national standards of the model curricula and to the purpose of innovative educational philosophy,with the years of continuous exploration and practice,the clinical immunology examination curriculum has kept developing from the teaching staff,teaching condition,content of course,teaching method and means etc.,which has brought about satisfactory teaching effects. Thus it is forming the model curricula with special features.

Objective To study the effect of Yuan‐source Points and Collateral‐points combination and Bushen Yishou cap‐sule on hemorheology and hs‐CRP in patients with cerebral infarction .Methods A total of 120 patients with neurological function defects diagnosed with cerebral brain infarction were randomly divided into 3 groups ,who were given general treatment and nursing as usual ,Acupuncture group in general treatment based on Yuan‐source Points and Collateral‐points combination for enjoy points principles ,to acupuncture treatment ;drug group to oral Bushen Yishou capsule;combined group to Yuan‐source Points and Collat‐eral‐points combination and oral Bushen Yishou Capsule .Neurological function defects scores ,blood rheological properties and blood hs‐CRP were evaluated in all patients before and after treatment for 1 month .Results Three sets of results in treatment of limb function after scoring ,hemorheology and serum hs‐CRP levels were significantly improved (P<0 .01) .There was better improve‐ment in Combined group than these in Acupuncture group and Drug group respectively (P<0 .01) .Conclusion Yuan‐source Points and Collateral‐points combination combined with Bushen Yishou capsule can significantly improve neurological function and hemo‐rheology ,hs‐CRP levels in patients with cerebral infarction .

ObjectiveTo investigate the influence of gender and psychosocial parameters on the quality of life in patients with irritable bowel syndrome(IBS). MethodsOut-patients from Jan to Oct 2008 in the Department of Gastroenterology at the First Affiliated Hospital of Nanjing Medical University were enrolled. Based on Rome teria IBS was diagnosed. Interviewed by a GI physician with questionnaire of demographic data,Zung self-rating anxiety and depression scale(SAS/SDS) and IBS-specific quality of life(IBS-QOL) questionnaire. Results452 patients were diagnosed as IBS according to Rome Ⅲ criteria. IBS was more common in female. In male, anxiety and depression scales were significantly increased( t =7. 191、6. 459 ,P ＜ 0. 01 ) and the scale of IBS-QOL was significantly decreased ( t =9.119, P ＜ 0.01 ) in female IBS patients. In IBS patients, IBS-QOL scales were negatively associated with the anxiety and depression scales(P ＜0. 01 ). ConclusionThese results suggest that there were gender differences in psychological status and quality of life in IBS patients,and difference psychological status based on gender should influence the quality of life of patients with IBS.

A polymer film of vitamin B1 was obtained at a graphite carbon electrode by cyclic voltammetric method in phosphate buffer solution (PBS). Cyclic voltammograms of the film electrode in PBS (pH 5) exhibited one pair redox waves, Epa=350　mV，Epc=325　mV，different from monomer vitamin　B1. The peak currents of poly-VB1 films was proportional to (scan rate)1/2, which showed that the charge transfer was controlled by diffusion in the polymer film. The Poly-VB1 modified electrode showed a good catalytic ability to dopamine and epinephrine. At the modified electrod, the peak potential for oxidation of dopamine occurred at 250 mV and 200 mV for epinephrine, while they were shifted towards the negative direction 100 mV and 300 mV respectively at the bare glassy carbon electrode . And their peak current had a linear relationship to the concentrations in the range of 8.0×10-7～1.0×10-4　mol/L for dopamine and 1.0×10-6～1.0×10-4　mol/L for epinephrine.

Objective To study dynamic changes of T cell subsets and CD3＋HLA-DR＋T cells in peripheral blood of composite tissue transplantation-hand allograft recipients. Methods The levels of CD3＋、CD3＋CD4＋、CD3＋CD8＋、CD3＋HLA-DR＋T cells and ratio of CD4/CD8 in peripheral blood of hand allograft recipients in different periods were examined by using flow cytometry.The recipients before transplantation were as the control groups.Results The first day after transplantation,the levels of CD3＋、CD3＋CD4＋、CD3＋CD8＋、CD3＋HLA-DR＋T cells and ratio of CD4/CD8 all began to descend.The 3th to 5th day after transplantation,the levels were the lowest.The 8th day,the levels of CD3＋、CD3＋CD4＋、CD3＋CD8＋、CD3＋HLA-DR＋T cells and ratio of CD4/CD8 were eventually rised and go to stable 15th day after transplantation.But the levels of CD3＋、CD3＋CD4＋and value of CD4/CD8 were still lower than those of the contols,and the levels of CD3＋CD8＋、CD3＋HLA-DR＋T cells were higher distinctly.Conclusion Dynamic changes of T cell subsets and active T cells in peripheral blood of composite tissue transplantation-hand allograft recipients were accordant with that of renal allograft recipients with stable period and with stable clinical state of the hand transplantation recipients.