Objective To observe the inhibitory effect of LaCl_3 on the growth of human hepatocellular carcinoma cell lines SMMC-7721 and its mechanism.Methods The cells were treated with different doses of LaCl_3 and the growth curve,colony inhibitory rates,the levels of AFP of cultured cells were detected at 1,3,5 and 7 days after they were dealed with LaCl_3.The changes of cell cycle were detected by flow cytometry and the expressions of cell cycle protein CyclinD_1,PCNA,CDK_4 and P16 were observed by immunocytochemistry staining.Results MTT test showed that SMMC-7721 cell proliterating activity was obviously inhibited by LaCl_3 in a time and dose related manner at concentrations of 0.50 and 1.00 mmol?L~(-1).The colony forming inhibitory rate of 0.10,0.50 and(1.00 mmol?L~(-1))LaCl_3 were 51%, 67% and 97% (P

13th Five-Year period is China's comprehensive completion of the decisive stage of a well-off society,when the development of public hospitals is facing great opportunities and challenges.Five development concepts introduced by the fifth Plenary Session of the 18th CPC Central Committee,offer guidance on public hospital reform and development.Among such concepts,innovation is leading the first power of hospital development;Coordination is the inherent requirements of the hospital' s sustainable and healthy development;Green is a necessary condition for the sustainable development of the hospital,and also an important reflection of the hospital development achievement;Opening is the only way for the rapid development of hospital;Sharing is the essential requirement of hospital development.Five development concepts shall guide the reform and development of Chinese public hospitals during the period of 13th Five-Year.

brain and fatty. The accumulation excretion ratios within 96 h were 70.16% in urine and only 1.35% in feces after iv administration in rats, while within 24 h was 3.46% in bile. Conclusion The results of pharmacokinetics of novel r-hirudin in rats given by iv determined by RA are different from those determined by TCA-RA, but the trends indicated by the two methods are the same; urine excretion is the major excretion pathway.

Objective To analyze hematosis of modified homemade Buxue decoction discriminate on patients with hemorrhagic anaemia after unilateral total hip arthroplasty and prognosis.Methods88 patients with hemorrhagic anaemia who underwent unilateral total hip arthroplasty in our hospital from February 2015 to Janurary 2017 were selected, they were randomly divided into the observation group and the control group, 44 cases in each group.The control group received pure western medicine after unilateral total hip arthroplasty, the observation group added modified homemade Buxue decoction discriminate on the basis of the control group.Clinical therapeutic effect on anemia, hematosis indexes including hemoglobin (HB), red blood cell (RBC), erythrocrit (HCT) in the two groups were compared, occurrence of complications during postoperative treatment, hip scores were used to compared prognostic differences in the two groups.ResultsTotal effective rate of the observation group 95.45% was significantly higher than the control group (81.82%) (P<0.05);there was no significant difference in Hb, RBC and HCT level before the treatment in the two groups, Hb (130.23±13.09)g/L, RBC (4.16±0.71)×1012/L and HCT (0.42±0.03) in the observation group after the treatment were significantly higher than the control group (P<0.05);complication rate in the observation group 9.10% was significantly lower than the control group (25.00%) (P<0.05);there was no significant difference in hip joint function scores before the treatment in the two groups, hip joint function score in the observation group after the treatment (79.73±5.42)scores was significantly higher than the control group (P<0.01).ConclusionTreatment effect of modified homemade Buxue decoction discriminate on hemorrhagic anaemia after unilateral total hip arthroplasty is significant, can promote recovery of hematosis, hip joint and motor function effectively.

Objective By discussing the relationship between allergic diseases caused by pollen,pollen density and meteorological factors,making out the warning for the allergic diseases caused by pollen,in order to provide a public service.Methods The data of meteorology from 2001 to 2005 of Tianjin,the pollen monitoring of the Weather Iron Tower Station day by day and the allergic patients whose skin tests were positive in Tianjin Municipal Chest Hospital and Tianjin Municipal Police Hospital were taken to statistical analysis on the rule of seasonal allergic diseases,the relationship between allergic diseases and pollen density and meteorological factors(such as vapor pressure and wind speed),and the prediction equations were obtained for allergic diseases caused by pollen.Results There was obviously positive correlation between the incidence rate of pollinosis(y),weighted pollen density(x11) and vapor pressure(x22) within twenty-days before the onset.The multiple correlation coefficient was 0.805 and the prediction equation was y =2.385+ 0.079 3x11 +3.507x22.Conclusion There is a close correlation between the incidence rate of pollinosis and pollen density.The allergic diseases caused by pollen are also affected by the change of air temperature,varieties of pollen and humidity.

AIM: In order to investigate the possible cumulative and chronic toxicity of paralytic shellfish poisoning (PSP) and provide more information on toxicity of PSP. METHODS: The sub-acute toxicity of PSP was evaluated in the rat. PSP was extracted from the seafood in market, and the toxicity of the extract was determined by mouse bioassay. The extracts in different toxicity were administrated to rats through gastrotube for 35 days. The biochemical and pathological changes in vital tissues in rats were examined by the detections of some function indexes in blood and urine and the observations under optical microscope during both the exposure period and the subsequent 10-day withdrawal term. RESULTS: No biochemical and pathological changes in tissues occurred for the control and low-dose group (4.6 ?g STX/kg), whereas some changes happened for the middle (9.2 ?g STX/kg) and high groups (18.4 ?g STX/kg). In experiment, some renal function indexes changed in the mid-dose group, and some of the cardiac, hepatic and renal functions indexes altered for the high dose group with some changes in weight of the thymus gland and spleen. What is more, the liver and kidney became indistinct with some inflammatory changes, and some muscles had ruptured for the 40% rats in high-dose group. CONCLUSION: These results suggest that long-term intake of PSP at a concentration, which doesn't exceed the threshold of standard, has some toxicity on rats, and that the toxicity of PSP has an accumulative effect. In a word, it is unsafe for us to eat frequently bivalve seafood polluted by PSP toxins.

Objective To investigate the correlation between pathologic features and contrast?enhanced ultrasound in patients with breast ductal papilloma. Methods From January 2014 to January 2016,80 patients with breast intraductal tumor treated in our hospital were selected,including 20 cases of breast ductal carcinoma (ductal carcinoma group) and 60 cases of benign tumor (benign group). Both groups received conventional ultrasound, ultrasonic angiography,and pathological feature analysis. Results Breast surrounding hyperecho,internal micro calcification,border edge burr and posterior echo attenuation were more frequently seen onconventional ultrasound in ductal carcinoma groupthan inbenign group(P<0.05). The average scores of contrast?enhanced ultrasound were significantly higher in ductal carcinoma group than in the control group(P<0.05). In the ductal carcinoma group, there were statistical significant differences betweenpatientswith different lymph node metastasis dukes staging ,and differentiation types (P < 0.05). Spearman rank correlation coefficient analysis showed that breast intraductal carcinoma lymph node metastasis and dukes staging,differentiation types were markedly correlated with pathological features and ultrasound imaging (r=0.341,0.368,and-0.289;P<0.05). Conclusions Conventional ultrasound and contrast?enhanced ultrasound have very good imaging featuresin the diagnosis of breast intraductal cancer ,and there is correlation between pathologic features and contrast?enhanced ultrasound in patients with breast ductal papilloma,which can provide values for early diagnosis of breast ductal carcinoma.

Objective To investigate the serovar distribution of Chlamydia trachomatis (Ct) isolated from male patients with urethritis in sexually transmitted disease (STD) clinic.Methods Urine specimens were collected from male patients with urethritis in STD clinic at Hospital of Dermatology,Chinese Academy of Medical Sciences between January 2013 and December 2013.Fluorescence-based quantitative PCR was performed to detect Ct DNA in these specimens.DNA was extracted from Ct-positive urine specimens,and nested PCR was conducted to amplify the VS1-VS2 regions of the outer membrane protein A (ompA) gene,followed by gene sequencing.The resulting sequences were aligned to reference sequences by the DNAStar5.0 software to determine Ct serovars.Results A total of 432 urine specimens were collected,and 33.1％ (143/432) of them were positive for Ct.The VS1-VS2 regions of the ompA gene were amplified from 127 out of the 143 Ct-positive specimens,but not from the other 16 specimens.Nine serovars were identified by gene sequencing among the 127 specimens,including serovar E (29 strains,22.83％),F (28 strains,22.05％),D (19 strains,14.96％),G (16 strains,12.60％),J (16 strains,12.60％),K (8 strains,6.30％),H (5 strains,3.94％),I (3 strains,2.36％) and B (3 strains,2.36％),and Ct serovars E,F,D,J and G accounted for 85.02％ among all the strains.Synonymous mutations were identified in 14 out of the 127 strains when compared with reference strains.Conclusions E,F,D and G serovars were the main Ct serovars in male patients with urethritis in STD clinic.The proportion of Ct serovar E strain was decreased,but that of serovar J strain was increased compared with 20 years ago.

Objective:To analyze the clinical characteristics of the patients with hyperthyroidism complicated with acute cerebral infarction, and to evaluate the prognosis and safety of intravenous thrombolysis treatment in the patients with hyperthyrodism complicated with acute cerebral infarctron within time window for thrombolysis. Methods:The clinical materials of patients with acute cerebral infarction treated with intravenous tissue plasminogen activator (rt-pA)were retrospectively analyzed.All patients were divided into hyperthyroidism group (n=41) and non-hyperthyroidismin group (n=160) according to whether complicated with hyperthyroidism.The general imformation,the area of cerebral infarction and intracranial hemorrhage of the patients in two groups were analyzed.Results:The NHISS score of the patients in hyperthyroidism group was higher than that in non-hyperthyroidism group for the first time(P<0.05).The NHISS scores 7 d after treatment and the 90 d modified Rankin scale (mRS) scores had no statistical differences between two groups (P>0.05).The proportions of the different infarction sizes had significant differences between two groups (P<0.05).The patients with large area cerebral infarction in hyperthyroidism group accounted for 51.2%, and it accounted for 27.5% in non-hyperthyroidism group.The incidence of asymptomatic intracranial hemorrhage of the patients in hyperthyroidism group was higher than that in non-hyperthyroidismin group (P< 0.05),but there was no statistical difference in the incidence of intracerebral hemorrhage (ICH) and subarachnoid hemorrhage (SHA) between two groups (P > 0.05).Conclusion:The patients with acute cerebral infarction complicated with hyperthyroidism could benefit from intravenous thrombolysis, without the increasing of incidence of spontaneous intracranial hemorrhage (sICH) and SHA.

Objective To retrospectively review the experience and early clinical result of carotid endarterectomy (CEA) for the treatment of symptomatic carotid artery stenosis. Method CEA was performed on 82 consecutive patients (66 males, 16 females), age ranged from 48 to 84 years (mean 68.6 years). Diagnosis was established by carotid artery Doppler ultrasonography and DSA in all patients. General anesthesia was used in 39 cases, regional anesthesia was used in 43 cases. Temporary intraluminal shunts were used in 56 patients. Results There was no surgical mortality. Postoperatively 76 patients were followed up from 4 months to 18 months. Symptoms significantly improved in 57 cases, mild improvement was achieved in 14 cases, severe postoperative complication occurred in 3 patients, including cerebral ischemia in 2 cases, intracarotid thrombosis in one. Conclusion CEA is an effective therapy for the treatment of symptomatic carotid artery stenosis.