Objective To explore the T‐SPOT .TB technology in latent tuberculosis infection (LTBI) who immunosuppres‐sive therapy results in screening for latent tuberculosis infection prevention and control to provide a new basis .Methods Applica‐tion of T‐SPOT .TB kit 162 immunosuppressed patients need to be applied to detect M .tuberculosis‐specific T cells ;while doing all cases tuberculin (TST ) skin test ;of which 28 cases of T‐SPOT .TB‐positive patients before screening technique using anti‐TNF‐αbiologics were given prophylactic treatment of anti‐TB drugs for 4 months and followed a year .Results The positive rates and ac‐curacy rate of T‐SPOT .TB assay were 36 .4% and 94 .9% ,while the positive rates and accuracy rate of TSTs were 28 .4% and 69 .6% .The difference between T‐SPOT .TB assay and TST were statistical significance(P < 0 .05) .Through our 28 cases of T‐SPOT .TB positive screening technology ,prophylactic anti‐TB drugs to treat patients for 4 months and 1 year of follow‐up ,no case of tuberculosis occurred .Conclusion These results demonstrate that the performance of T‐SPOT .TB is better than the classic TST for detection of LTBI in patients receiving immunosuppressive therapy for treatment of systemic autoimmune disorders .The T‐SPOT .TB assay will be a useful tool in early and rapid diagnosis of latent tuberculosis infection .T‐SPOT .TB for LTBI patients di‐agnosed with prophylactic anti‐TB drug treatment is necessary ,has important clinical significance .

The adjustment of Laboratory Medicine to the first class subject is both challenges and opportunities. In order to meet the requirements of the modern laboratory medicine on the improved knowl-edge and skill, Weifang Medical University sets a goal of cultivatinghigh-qualified and applicative profes-sional personnel of Laboratory Medicine Science. To realize that, we build a new curriculum system with features of Medical Laboratory Science, including the theoretical curriculum system and the practical teach-ing system. The theoretical curriculum system breaks the traditional framework of the old curriculum which designed according to the discipline category, builds a new curriculum system of platform and module, scientifically integrates the basic course group in clinical laboratory medicine, and adds the course of clinical examination skills with its own professional characteristics. In the practical teaching system, we focus on the cultivation of practice ability and innovation ability, encourage the students to go to the hospital and company earlier, and promote the integration of production, teaching and research in various approaches.

Rabies is a deadly zoonos caused by rabies virus, the case fatality almost is 100%following suffering from this dis-ease.Recently, the incidence of rabies tends to increase in China.A rapid, accurate, economical, simple and convenient, easy to be widely used method for rabies virus detection in laboratory is urgently needed.This paper reviews the nucleic acids diagnosis techniques of rabies virus from the nucleic acids detection methods based on PCR, NASBA, LAMP, Genechips and analyzes the advantages and disadvantagesof the above-mentioned methods, which provides a reference for rapid laboratory diagnosis of rabies virus to efficently prevent and control rabies.

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis .The rapid development of laboratory diagnostic technology provides a variety of options for diagnosis and treatment of tuberculosis .Nucleic acid detection is to use the theory and technology of molecular biology ,through direct detection of status or defect of particular segments ,then make diagnosis of the state of human body and disease .In recent years ,the development of nucleic acid diagnostic technique has greatly promoted the rapid diagnostic of laboratories .This article reviews the new methods of bacterial nucleic acid and host mi‐croRNAs detection technologies .

Interaction in classroom teaching is a bilateral activity that is difficult to control. Teachers and students are the two main aspects to realize the synchronization of teachers and students' thinking. For teachers, teachers should first make full use of multimedia resources, bring a colorful courseware into class-room teaching; secondly, teachers should stimulate students' interest in a reasonable way, so as to inject vitality into the classroom teaching; finally, teachers should mobilize the students' thinking and carefully regulate the changes in thinking between teachers and students through a proper teaching methods. In terms of students, students should not only give full play to their initiative, but also actively cooperate with teachers in order to realize the thought synchronization between teachers and students in classroom teaching and learning interaction and ultimately achieve the desired results.

Objective To amplify the higA gene from the Mycobacterium tuberculosis,and to analyze the structure and function of their encoded proteins by using bioinformatics.Methods Total DNA was extracted from Mycobacterium tuberculosis.PCR of higA was performed and the products were sequenced.The biological features of the higA protein including,its physical and chemical properties,signal peptide,spatial structure and epitopes were analyzed by using software online.Results The PCR products of higA were 450 bp in length,which were consistent with the expected size.The higA protein consisted of 149 amino acids and had the following characteristics:a theoretical isoelectric point of 7.93,a fat-soluble factor of 94.30,and instability coefficient of 36.57.The higA protein had no signal peptide,containing 10 phosphorylation sites and multiple potential epitopes.Conclusion Mycobacterium tuberculosis higA gene can be amplified by PCR and the characteristics of higA protein is identified.

ABSTRACT:The aim of the present study was to investigate the inhibitory effects of TLR7 on Mycobacterium tuberculosis . TLR7 on infected RAW264 .7 cells was activated by chemical synthesis of TLR7 activation motif ssRNA .Activated RAW264 .7 cells were inoculated with Mycobacterium tuberculosis ,quantitative PCR method was applied to detect the phagocytosis rate of cell to bacteria at different time after infection .Cytokine production was measured by ELISA from cell supernatant .Cells were cultured on Roche medium and counted after sterile cracked with TritonX‐100 and diluted with PBS .Scanning electronic micro‐scope ( SEM ) was applied to detect the morphological changes of cells treated with TLR7 activation motif ssRNA .The highest phagocytosis rate of bacteria of RAW264 .7 cells was at 3 hours post infection (P>0 .05) .Compared with that of the control group ,treatment after 36 hours intracellular bacterial quantity in ssRNA treated group was lower (P<0 .05) ,levels of IL‐12 (P<0 .05) and IL‐4 (P<0 .05) were increased .For treatment after 48 hours ,level of IL‐4 (P<0 .05) was decreased ,and TNF‐α (P<0 .05) was increased .For treatment after 3 hours ,cell morphology of the ssRNA group was obviously better than the control group and appeared lots of phagosomes .Results suggested that TLR7 could enhance macrophages in killing Myco‐bacterium tuberculosis by forming phagosomes and regulating cytokines production ,and TLR7 activation motif ssRNA could be used in the treatment of tuberculosis .

The culture of modern medical talents is the requirements of the times on cultivating talents of Medical Laboratory of Medical College. In order to establish a high level teaching team and im-prove the quality of teaching and personnel training, through the reform of the traditional teacher training way, we created the teacher training mode of multi echelon teachers including department, university, domestic and international training ways. New curriculum teaching system framework was established which was based on theoretical teaching, practical teaching and scientific research. Besides, innovative and pro-gressive practice teaching platform was set up which included basic experiment, clinical skills, professional training, and practice in company. Course of Clinical Examination Skills was opened. The teaching team achieved some results in the above teaching reform.

Construction of course group,which overcomes the narrow limits of knowledge structure for single course,carries training objectives of comprehensive knowledge and innovation ability,and plays an important role in realizing the goal of higher medical education.Basic course group in clinical laboratory medicine has been built based on the close relation,promotion,and infiltration of five courses contents.It has been explored and reformed from the restructuring and optimizing of course group contents,teaching methods,practice step,and so on.Practice shows that the course group is helpful to improving students' ability of integrated use of knowledge and presents obvious effect for improvement of the cultivation of students' comprehensive quality.

Objective:To explore molecule mechanism about anti-aging of bifidobacterium by investigating the effect of lipoteichoic acid (LTA) of bifidobacterium on thymus of aging mice induced by D-galactose.Methods:Male Kunming mice were divided into different groups at random. These mice were induced aging by subcutaneous injection of D-galactose on the back of mice and LTA was simultaneously administered to them daily for 40 days. Body weight, thymus index ,thymus morphology, thymus c-fos, p16 production and peripheral blood IL-2 concentration were tested by HE staining, immunochemistry method, ELISA and other methods. Results:There were significant differences for body weight ,thymus index, thymus morphology,thymus p16, c-fos production and peripheral blood IL-2 concentration between the young control group and the aging model group. Whereas, LTA can improve thymus morphology ,increase body weight ,thymus index and c-fos production, meamwhile, decrease thymus p16 and peripheral blood IL-2 concentration of aging model mice. Conclusion:The results showed that LTA can remarkably delay thymus aging, and it may be a molecular mechanism of bifidobacterium delaying immunosenescent.