Objective:To evaluate the effect of subconjunctival injection of Bevacizumab(Avastin) on corneal neovascularization(CNV) in rabbits.Methods:Forty-eight New Zealand rabbits were randomly divided into three groups:normal control group(group A,3 rabbits),neovascularization group(group B,9 rabbits) and Bevacizumab treatment group(group C,36 rabbits).Group C was further divided into group C1(1 day small dose group),C2(1 day high dose group),C3(14 days small dose group) and C4(14 days high dose group),with 9 rabbits each group.CNV model was made by suture in group B and C.Animals in group C were injected subconjunctivally with 0.1 ml or 0.2 ml Bevacizumab(25 mg/ml) in the left eyes.The growth of CNV was observed every day after operation and the neovascularization areas calculated.Expression of VEGF in the cornea was detected by immunohistochemistry on day 7,14 and 28 after suture.VEGF content in the aqueous humor was determined by ELISA assay.Results:CNV growth in group C1 and C2 was inhibited significantly compared with that in group B on day 7,14 and 28(P

As the patient of hepatitis B and E overlapping infection of liver and gallbladder damp - heat type compared with the patient of simple hepatitis E of liver and gallbladder damp-heat type, attack was very rapid, jaundice exacerbated progressively and hepatic function damaged severely. Aftertreatment the state of illness restored more slowly and the duration of illness was obviously prolonged. At the same time, it was found that the biochemical indexes (hyaluronic acid, total bile acid) increased significantly at acute stage, and the indexes in the patient of overlapping infection in the time for recovering normality at restoration stage was longer than that in the patient of simple hepatitis E, with a significantly difference between the two groups. It is indicated that lesion of the liver in the patient of overlapping infection of hepatitis B and E was more severe than the simple hepatitis E, with no good prognosis.

Objective:To study the clinical effect of Yang Supplementing Five Returning Decoction(YSFRD) on diabetic peripheral neuropathy. Methods: The curative effect of YSFRD on diabetic peripheral neuropathy and its influence on nerve conduction were comparatively studied. Results: YSFRD was superior to the control group in the curative effect on diabetic peripheral neuropathy ( P

Objective To study the change of endothelial function during myocardial ischemia-reperfusion injury in rabbits and the effect of Rasuvastatin. Method Sixteen New Zealand rabbits were randomLy divided into two groups: ischemia/reporfusian injury group (control group) and Resuvastatin group(drug group). The myocar-dial ischemia-reperfusion model was established by occlusion of left anterior descending coronary artery for 40 min-utes evidenced by the elevation of the ST segment≥0.2 my on ECG waveform, and after release of ooclusion, the ST segment of ECG retttmed to 1/2 or more of the normal wavefonn, which was the evidence of successful reperfu-sion. The rabbit serum nitric oxide (NO) and plasma endothelia-1 (ET-1) content were assayed before occlusion, 40 minutes, 60 minutes and 180 minutes after reperfusion. SPSS11.5 software was used for ANOVA(Repested Measurement designs). P<0.05 was considered as statistically significant. Results There were no significant differences in serum NO and plasma ET-1 between two groups bsfore isehemia, but 40 minutes, 60 minutes and 180 minutes after reperfusion, the levels of serum NO in drug group were higher than those in control group [(82.000±13.825), (63.375±17.541), (50.250±18.987)μmol/L vs. (63.125±18.962), (43.500± 16.518), (29.625±14.162) μmol/L, P<0.05], and the levels of plasma ET-1 content in drug group were lower than those in the control group [(282.541±38.928), (315.152±55.263), (377.795±60.427) pg/mL vs. (331.785±35.341),(375.914±5.204),(459.829±70.110) pg/mL, P<0.05]. Conclusions By the means of increasing serum NO and decreasing plasma ET-1, rosuvastatin can improve the endothelial function in rabbits with isehemia-reperfusion injury.

Objective To systematically review ICU-nurse lightly sedated patients, in order to provide evidence for sedation. Methods Databases were searched including The Cochrane Library, Joanna Briggs Institute Library, PubMed, EMbase, Ovid, ELSEVIER CMB, CNKI and WANFAN from inception to May 2018, to collect qualitative studies in the experience of ICU nurses lightly sedated patients. The quality of included studies was evaluated according to JBI Critical Appraisal Tool for qualitative studies in Australia. The results were integrated by integrating methods. Results A total of seven studies were included. Thirty-five complete findings were grouped according to their similarities to form six categories. These categories resulted in two synthesized findings: Despite the complexity and unknowns of care, critical care nurses were slowly accepting the fact and try to cope with; there were some barriers on the implementation of sedation, there should have a multi-party collaboration. Conclusions the heath management system should pay more attention to implementing light sedation strategies, ICU-nurses should be given support, guidance and encouragement.

To summarize the experience of perinatal nursing for 27 days of delayed delivery of the second fetus of a twin pregnancy with placenta previa.Key nursing points include:the start of high risk warning during delayed delivery,and being alert to placenta previa hemorrhage;effective prevention of infection,reducing the incidence of maternal sepsis and chorioamnionitis;actively inhibiting the contractions to prolong gestational age and improving the perinatal survival rate of the second fetus;strengthening in utero fetal monitoring,identifying the early signs of fetal distress;targeted implementation of personalized psychological nursing,to ensure the mental health of patients.The rescue plan during delivery should be improved to ensure the safety of mother and child.Postpartum prevention and treatment of massive bleeding was actively conducted,as well as the prevention of puerperal infection.After 79 d of active treatment and intensive nursing care,the patient was treated and discharged from the hospital without any signs of infection or a massive bleeding.The second newborn,whose delivery was delayed,was discharged from the hospital 59 d after birth,and the follow up was continued for a month until the reach to the standard growth development parameters and the overall physical examination was good.

Mouse cortical radial glial cells (RGCs) are primary neural stem cells that give rise to cortical oligodendrocytes, astrocytes, and olfactory bulb (OB) GABAergic interneurons in late embryogenesis. There are fundamental gaps in understanding how these diverse cell subtypes are generated. Here, by combining single-cell RNA-Seq with intersectional lineage analyses, we show that beginning at around E16.5, neocortical RGCs start to generate ASCL1