This paper reported a rapid hydrolysis method of food protein using microwave oven and high pressure vessel technique. Tryptophan in hydroly-zate was determined by fluorimetric spectroscopy method. The hydrolytic agent was 5 M NaOH solution. Under the operative condition of the microwave oven (microwave output power 650W, 2 minutes 15 seconds and 65W 2 minutes) the highest tryptophan contents of the pure protein lysozymum and food SRM liver, cabbage and wheat flour were obtained. The results of the t-test showed that there was no significant difference between the tryptophan contents determined by using the operative condition of the microwave oven mentioned above and 145℃ 4h hydrolysis method. The recovery of the method was 93.3%-107%, and the coefficient of variation obtained by parallel determination of 10 lysozymum sample was 4.2%.

As the patient of hepatitis B and E overlapping infection of liver and gallbladder damp - heat type compared with the patient of simple hepatitis E of liver and gallbladder damp-heat type, attack was very rapid, jaundice exacerbated progressively and hepatic function damaged severely. Aftertreatment the state of illness restored more slowly and the duration of illness was obviously prolonged. At the same time, it was found that the biochemical indexes (hyaluronic acid, total bile acid) increased significantly at acute stage, and the indexes in the patient of overlapping infection in the time for recovering normality at restoration stage was longer than that in the patient of simple hepatitis E, with a significantly difference between the two groups. It is indicated that lesion of the liver in the patient of overlapping infection of hepatitis B and E was more severe than the simple hepatitis E, with no good prognosis.

Objective To explore the effect of cell apoptosis and oxidative stress on stressive liv-er injury after traumatic brain injury (TBI) in rats. Methods The model of TBI was duplicated by u-sing modified Allen's mehtods. Forty male Wistar rats were randomly divided into control group and groups at 6,12,24,48 hours after TBI. The serum levels of ALT and AST as well as the levels of superox-ide dismutase (SOD) and malandialdehyde in liver tissue were measured. The index of hepatocyte apopto-sis was detected through flow cytometer. Pathological changes of liver tissues were observed under light and electron microscopes. Results After TBI, the serum levels of ALT and AST were significantly in-creased, while malondialdehyde was increased and SOD decreased in liver tissues. The electron micro-scope showed that the index of hepatocyte apoptosis reached a peak at 6 hours after TBi. Aggressive inju-ries of the liver tissues were observed after TBI, showed by pathological observations. Conclusion Cell apoptosis and oxidative stress may be involved in the pathogenesis of stressive liver injury after TBI.

Objective To study the change of endothelial function during myocardial ischemia-reperfusion injury in rabbits and the effect of Rasuvastatin. Method Sixteen New Zealand rabbits were randomLy divided into two groups: ischemia/reporfusian injury group (control group) and Resuvastatin group(drug group). The myocar-dial ischemia-reperfusion model was established by occlusion of left anterior descending coronary artery for 40 min-utes evidenced by the elevation of the ST segment≥0.2 my on ECG waveform, and after release of ooclusion, the ST segment of ECG retttmed to 1/2 or more of the normal wavefonn, which was the evidence of successful reperfu-sion. The rabbit serum nitric oxide (NO) and plasma endothelia-1 (ET-1) content were assayed before occlusion, 40 minutes, 60 minutes and 180 minutes after reperfusion. SPSS11.5 software was used for ANOVA(Repested Measurement designs). P<0.05 was considered as statistically significant. Results There were no significant differences in serum NO and plasma ET-1 between two groups bsfore isehemia, but 40 minutes, 60 minutes and 180 minutes after reperfusion, the levels of serum NO in drug group were higher than those in control group [(82.000±13.825), (63.375±17.541), (50.250±18.987)μmol/L vs. (63.125±18.962), (43.500± 16.518), (29.625±14.162) μmol/L, P<0.05], and the levels of plasma ET-1 content in drug group were lower than those in the control group [(282.541±38.928), (315.152±55.263), (377.795±60.427) pg/mL vs. (331.785±35.341),(375.914±5.204),(459.829±70.110) pg/mL, P<0.05]. Conclusions By the means of increasing serum NO and decreasing plasma ET-1, rosuvastatin can improve the endothelial function in rabbits with isehemia-reperfusion injury.

Objective To systematically review ICU-nurse lightly sedated patients, in order to provide evidence for sedation. Methods Databases were searched including The Cochrane Library, Joanna Briggs Institute Library, PubMed, EMbase, Ovid, ELSEVIER CMB, CNKI and WANFAN from inception to May 2018, to collect qualitative studies in the experience of ICU nurses lightly sedated patients. The quality of included studies was evaluated according to JBI Critical Appraisal Tool for qualitative studies in Australia. The results were integrated by integrating methods. Results A total of seven studies were included. Thirty-five complete findings were grouped according to their similarities to form six categories. These categories resulted in two synthesized findings: Despite the complexity and unknowns of care, critical care nurses were slowly accepting the fact and try to cope with; there were some barriers on the implementation of sedation, there should have a multi-party collaboration. Conclusions the heath management system should pay more attention to implementing light sedation strategies, ICU-nurses should be given support, guidance and encouragement.

Objective To compare drying methods for dental handpiece,and to provide references and guidance for choosing appropriate handpiece drying methods.Methods Four groups were established according to different methods of drying:vacuum drying,compressed air dryer drying,entire automatic hot cleaner drying,and sterilizing machine drying.Four types (with 50 in each type)of handpieces with the same brand were selected:low-speed straight (EX-6C),low-speed bender (NAC-EC),high speed turbine fast handset,and high speed turbine four hole handset.After cleaning and disinfection,each handpiece was subjected to the aforementioned four drying methods,and the mass was recorded before and after drying.SPSS software was used to calculate and compare the drying rate among these drying methods by analysis of variance.Results There were significant differences in the drying rate among four drying methods(P＜0.01).The drying efficiency with vacuum drying was higher than the other three methods.The mean drying efficiency for four types of dentistry handset with vacuum drying method were as follows:low-speed straight(EX-6C)69.93％,low-speed bender(NAC-EC)93.21％,high speed turbine fast handset 92.70％,and high speed turbine four hole handset 94.27％.Conclusion The drying efficiency of vacuum drying method for handset is superior to the other three drying methods.

Objective To develop an accurate deep learning prediction model of YOLO-V5 capable of accurately iden-tifying medication packaging boxes in outpatient and emergency pharmacies,aiming to assist pharmacists in achieving"zero dis-pensing error".Methods A total of 2 560 images of packaging boxes from 136 different drugs were collected and labeled to form the deep learning dataset.The dataset was split into training and validation sets at a ratio of 4∶1.YOLO-V5 deep-learning algorithm was employed for training the data using images from our dataset(train epochs:500,batch size:4,learning rate:0.01).The values of the precision(Pr)and mean average precision(mAP)were used as measures for model performance evaluation.Results The Pr of the four sub-models of YOLO-V5 in the training set all reached 1.00.The mAP_0.5 of YOLO-V5x was 0.95,which was higher than those of YOLO-V5s(0.94),YOLO-V5l(0.94),and YOLO-V5m(0.94).The mAP_0.5:0.95 of YOLO-V5l and YOLO-V5x were 0.85 which were higher than those of YOLO-V5s(0.84)and YOLO-V5m(0.84).Training time and model size were 82.56 hours and 166.00MB for YOLO-V5x which were the highest among the four models.The speed of detection in one im-age was 11ms for YOLO-V5s which was the fastest among the four models.Conclusion YOLO-V5 can accurately identify the packaging of drugs in outpatient and emergency pharmacies.Implementing an artificial-intelligence-assisted drug dispensation sys-tem is feasible for pharmacists to achieve"zero dispensing error".

Objective To preliminarily construct a training program of dental nurse specialism so as to provide a theoretical basis for the training of dental nurse specialism. Methods The consultant questionnaire was established preliminarily. The training program was constructed with the Delphi expert consultation method involved 16 experts by two rounds of consultation. Results The experts were with high active coefficient and authority coefficient. The training program was confirmed including training targets, access conditions, cultivation mode, faculty, setting of training course, examination forms and qualification certification. Conclusions The training program of dental nurse specialism explored with the Delphi method is with well scientific nature and authority. It has a reference value for the training of dental nurse specialism.

Objective:To explore the application effects of mind mapping combined with scenario simulation training on the ability training of junior nurses in hospital transfer of patients with critical burns and trauma.Methods:A prospective randomized controlled study was conducted. From December 2019 to December 2020, 55 female junior nurses from the Institute of Burn Research of the First Affiliated Hospital of Army Medical University (the Third Military Medical University) who met the inclusion criteria were enrolled in this study and divided into routine group (27 nurses, aged (24.0±0.9) years) and combined group (28 nurses, aged (24.2±0.8) years), according to the random number table. The nurses in routine group were trained with hospital transfer of patients with critical burns and trauma by theory combined with operational skill, and the nurses in combined group were trained with hospital transfer of patients with critical burns and trauma by mind mapping combined with scenario simulation training. Before and after the training, the self-made theoretical examination papers and skill assessment items were used for the examination and assessment to nurses, and their scores were calculated and compared. The self-made emergency ability scoring system was used to evaluate the emergency disposal ability of nurses from five dimensions, including team cooperation ability, emergency response ability, operative technique ability, specialized business ability, and nurse-patient communication ability, and their scores were calculated and compared. The non-standard implementation rates of transfer nursing measures, such as incomplete preparation of goods, poor communication effect of patients, inadequate pipeline nursing, unclear handover, and inadequate final treatment, were calculated and compared in the process of transporting highly simulated human (hereinafter referred to as simulated human) by nurses before and after training; and the rate of disease change and successful rate of transport of simulated human were calculated and compared after training. After assessment, self-made satisfaction questionnaire was used to compare nurses' satisfaction with the training mode, content, and effects. Data were statistically analyzed with independent sample t test, Pearson chi-square test, or Yates corrected chi-square test. Results:Fifty-five enrolled nurses were fully involved in the training, examination, assessment, and questionnaire filling. Before training, there were no statistically significant differences in theoretical examination and skill assessment scores between the 2 groups ( P>0.05); After training, the theoretical examination and skill assessment scores of nurses in combined group were significantly higher than those in routine group (with t values of -3.89 and -4.24, respectively, P<0.05). Before training, there were no statistically significant differences in the scores of each item of emergency disposal ability between the 2 groups ( P>0.05); after training, the scores in terms of team cooperation ability, emergency response ability, operative technique ability, specialized business ability, and nurse-patient communication ability of nurses in combined group were significantly higher than those in routine group (with t values of -6.49, -6.44, -2.21, -2.85, and -2.34, respectively, P<0.05). Before training, there were no statistically significant differences in the non-standard implementation rates of transfer nursing measures of nurses between the 2 groups ( P>0.05); after training, the non-standard rates of incomplete preparation of goods, unclear handover, and inadequate final treatment of nurses in combined group were significantly lower than those in routine group (with t values of 3.87, 5.89, and 5.28, respectively, P<0.05). After training, the rate of disease change of simulated human of nurses in combined group was 7.14% (2/28), which was significantly lower than 33.33% (9/27) in routine group ( χ2=5.89, P<0.05); the successful rate of transport was 96.43% (27/28), which was significantly higher than 74.07% (20/27) in routine group ( χ2=3.87, P<0.05). After assessment, the total score of training satisfaction and scores of satisfaction with training mode and training effect of nurses in combined group were significantly higher than those in routine group (with t values of 5.22, 4.67, and 10.71, respectively, P<0.05). There was no statistically significant difference in the satisfaction score on training content between the two groups ( P>0.05). Conclusions:Evidence-based mind mapping combined with scenario simulation training significantly improves the nursing skills and emergency handling capabilities of junior nurses in transferring patients with critical burns and trauma.

Mouse cortical radial glial cells (RGCs) are primary neural stem cells that give rise to cortical oligodendrocytes, astrocytes, and olfactory bulb (OB) GABAergic interneurons in late embryogenesis. There are fundamental gaps in understanding how these diverse cell subtypes are generated. Here, by combining single-cell RNA-Seq with intersectional lineage analyses, we show that beginning at around E16.5, neocortical RGCs start to generate ASCL1