Objective To evaluate the utility of the Chinese version of the Edinburgh postnatal depression scale(EPDS)when being used in puerperas in Chengdu.Methods Following translation and back translation,the EPDS was commented by six experts,then a prospective cohort of 732 women completed the EPDS and the Beck depression inventory(BDI)three to fourteen days after siring birth.Rusuits The concordance rates of translation and back translation were all 100%;the content validity ratio of the EPDS was 0.9333;the Cronbaeh's alpha coefficient of the EPDS was 0.76,the cumulative variance contribution of the two factors was 44.979%,and the spearman correlation coefficient between the EPDS and BDI was 0.584,＜0.01.Conclusions The EPDS possesses good cknical pefformanee,is very concise,easy to read and understand and well accepted by mainland Chinese puerperas and researchers.It is a good instrument for screening for postnatal depression in puerperas of Chengdu.

Objective To compare the rat and mice intestine slides quality fixed with three different fixatives and embeded by paraffin.Methods Three kinds of conventional fixatives were selected for the test, they are 10% neutral buffered formalin,Bouin’s solution and modified Davidson’ s solution.SD rat’ s and KM mice’ s intestinal tissues were fixed with the three different fixatives, then embeded, sliced and stained with HE as routine rut.To observe the slides quality under microscope.Results Sheding and necrosis of intestine villus epithelia were observed in slides made from formalin fixed intestines, which is speculated due to the fixations.The phenomenon was not observed in Bouin's solution and modified Davidson’ s solution fixed tissues.And the tissue structure were more fine and clear in them.Conclusions The rat and mice intestine slides made from tissues fixed by Bouin's solution and modified Davidson's solution are better than from the 10%neutral buffered formalin fixed ones.

Objective The study was designed to evaluate the clinical efficacy of azithromycin in lung function of patients with IPF.Methods Randomized controlled trials were searched in CNKI,data-base of Wanfang and WeiPu,where azithromycin was used to patients with IPF.We extracted all data on lung functions,standardized mean difference (SMD)with 95% confidence intervals (CI )were pooled with Stata 11.0.Results Seven unique trials with 516 participants were identified.Be compared to pred-nisone,the patients with IPF received azithromycin experienced no improvement in FVC% [SMD = -2.00,95% CI =(-2.05,-1.95)],but it was found that azithromycin was superior to prednisone in in-crease the percentage of carbon monoxide diffusing [SMD =1.16,95% CI =(-2.83,5.16)]and de-cline the percentage of residual volume[SMD =3.00,95% CI =(1.04,4.96)].Azithromycin combined with prednisone were statistically superior to prednisone alone in improvement of FEV1 %[SMD =7.80, 95% CI =(5.92,9.67)],FVC%[SMD =7.23,95% CI =(4.89,7.56)]and lung carbon monoxide diffusion function[ml/(min·kpa))][SMD =40.62,95% CI =(38.96,42.28)].Conclusions Our study suggests that azithromycin significantly improvement pulmonary diffusion function in patients with IPF. Azithromycin combined with prednisone were more effective in improvement of FEV1 %,FVC% and lung diffusion function.

Objective To study the expression of survivin protein in primary hepatocellular carcinoma(PHC) and its relationship to the proliferation of the tumor cells and prognosis of PHC. Methods The expression of survivin protein and the proliferation of tumor cells marked by proliferating cell nuclear antigen (PCNA) in 48 cases of PHC were determined by immunohistochemical method. Results The survivin protein was expressed in 31 of 48 cases of PHC (64.6%). The expression of PCNA was significantly higher in hepatocellular carcinoma (HCC) with positive survivin expression than in HCC with negative survivin expression. The patients with positive survivin expression had the worse prognosis than those with negative survivin expression. Conclusion The expression of survivin may play an important role in the proliferation of PHC cells and closely associate with the prognosis of PHC, and probably become the prognostic factor and an important target of therapy.

Objective　To investigate the possible roles of the different subnuclei of hypothalamic paraventricular nucleus （PNV） which may regulate splenic immune function. Methods　4 SD rats received splenic injection of pseudorabies virus（PRV）.96 hours after injection， a block containing hypothalamus was taken and cut into 40 μm sections with cryostat. Immunohistochemical technique and stereology method were used to detect the distributing character of transsynaptic PRV infection neurons in the PVN and the localization relationship between PRV neurons and AVP neurons in the PVN.Results　Transsynaptic PRV infection neurons distributed through out the PVN， but mainly concentrated in the lateral parvocellular （LP） subnucleus of PVN and posterior magnocellular （PM） subnuclei of PVN （occupied 73.49％ of total surface area），a few distributed in the anterior， dorsal and periventricular parvocellular （AP，DP and PP）nuclei of PVN，few scatted in the rest of nuclei of PVN. In the LP and PM some of the PRV neurons distributed among the AVP neurons.Conclusions　Besides the classical neuro-immuno-endocrine modulation， the PVN may regulate the splenic immune function through direct innervation .The AVP neurons in the PM and neurons projecting to the proganglionic neurons in the dorsal medulla and lateral horn of spinal cord in the LP，DP and AP regulate the immune function of spleen through sympathetic and para-sympathetic innovation.

Objective To investigate the multi-resistance to antibiotics of clinical isolated klebsiella pneumoniae.Methods The resistance to antibiotics of clinical isolated klebsiella pneumoniae were monitored.The discconfirmatory test was used to detect extended-spectrum β-lactamases(ESBLs) and cefoxitin three-dimension was used to detect AmpC β-lactamases.Results Among the isolates there were 53 strains of ESBLs-producing bacteria (49.5% ), 30 strains of AmpC-producing bacteria(28.0%), 24 strains of ESBLs + AmpC-producing bacteria (22.46%).They were high resistance to aminoglycosides,quinolones and cephalosporins.Conclusion The multi-resistance to antibiotics of clinical isolated klebsiella pneumoniae were widespread.It is important to control nosocomial infection to strengthen the detection of the epidemiology of ESBLs and AmpC β-lactamases in clinical isolates.

Objective To investigate the influence of hospital-community integrated management mode on self behavior and blood glucose level in patients with typeⅡdiabetes .Method Three hundred and thirty-six patients with type II diabetes were treated with hospital-community integrated management. The index of biochemical indicators and self behaviors were compared between pre-and post-intervention.Result After intervention,the patients had improvements not only in medication compliance,rationale dieting,regular examination and self monitoring and physical exercises,but also in the indexes of biochemical indicators including fasting blood glucose(FBG),2 hour postprandial blood glucose(2 hBG), glycosylated hemoglobin(HbA1 c),(all P<0.05).Conclusion The hospital-community integrated management mode is helpful for the patients to form a right behavioral pattern and thus promoting the normalizations of biochemical indicators.

Objective To investigate the family dynamic characteristics of adolescents with emotional disorder.Methods 74 adolescents with emotional disorder from Child Mental Health Research Center of Nanjing Brain Hospital Affiliated to Nanjing Medical University were chosen and compared with 148 gender- and agematched controls from schools in Nanjing.Self-rating scale of systemic family dynamics(SSFD) was used to assess the characteristics of family dynamics,and general demographic information inventory was conducted for all subjects.Results Adolescents with emotional disorder showed higher scores in family atmosphere and systemic logic than control group ( ( 30.54 ± 8.28 ) vs (23.45 ± 7.40),( 17.14 ± 4.26 ) vs ( 15.43 -± 3.86 ),all P ＜ 0.01 ).Logistic regression analysis showed that high individuation ( OR =0.903,95％ CI:0.834 ～ 0.977 ) and high disease thinking( OR =0.853,95％ CI:0.750 ～ 0.970 ) were protective factors for emotional disorder.High family atmosphere ( OR =1.167,95％ CI:1.101 ～ 1.236) was risk factor for emotional disorder.Conclusion Adolescents with emotional disorder demonstrate boring and hostile family atmosphere and Either/or family systemic logic on the family dynamic characteristics.Family atmosphere,disease thinking and individuation are associated with emotional disorder.

Objective To explore the status of stress to the life affairs between Tibetan nursing students on 9+3 free education programs and mainland nursing students.According to the diversity of them,corresponding nursing interventions of mental health were put forward.Methods The questionnaire of Adolescent Self-rating Life Events Check List(ASLEC)was distributed to 120 nursing students in each group through random sampling,and the SPSS 17.0 statistical software package was used for data analysis.Results The scores of Tibetan nursing students were higher than mainland nursing students on factors of study pressure,personal relationship and health adaptation,which showed significant difference.The results of ASLEC showed that the scores of personal relationship,deserved punishment and loss in the Tibetan nursing students of 1st gradewere significantly higher than those in the Mainland nursing students of 1st grade score.The scores of study pressure in the Tibetan nursing students of 2nd grade were significantly higher than those in the Mainland nursing students of 2nd grade.The scores of personal relationship and health adaptation in Tibetan nursing students of 3rd grade were higher than those in the Mainland Nursing Students of 3rd grade score.Conclusions Influence of stress to the life affairs on psyche of Tibetan nursing students is outstanding in contrast to Mainland nursing students,and Tibetan nursing students were given directed intervention of mental health problem according to their risk factors of stress to the life affairs.