BACKGROUND: Moxonidine is the second-generation high-selective central antihypertensive drug, while clonidine is the first-generation antihypertensive drug that is used in clinic with many side effects.OBJECTIVE: To compare the characteristics between moxonidine and clonidine in renal-hypertensive rats.DESIGN: Randomized controlled animal experiment.SETTING: Department of Pharmacology, Medical College, Nantong University.MATERIALS: The experiment was conducted in Medical College, Nantong University between September and December 2004. Totally 110 SD rats aged 60 days with the body mass of (180±30) g were used in the study.METHODS: Left renal artery stenosis in SD rats was established by inserting silver clip with the inner diameter of 0.2 mm or 0.25 mm, while the right renal artery was not received, so as to establish two-kidney one-clip(2K-1C) renal hypertensive models. ①Changes of blood pressure and heart rate in awake rats with renal hypertension were determined with arteria caudilis indirect manometric method, oral administration once or consecutively. The experiment of depressurization with once oral administration:The rats were randomly assigned into 5 groups with 10 rats in each group:1 mg/kg, 3 mg/kg, 10 mg/kg moxonidine hydrochloride groups, 1 mg/kg clonidine hydrochloride group were considered as positive control group,while saline group as negative control group. On the basis of the effect of moxonidine hydrochloride on blood pressure, blood pressure was measured at 1, 4, 24, 48, 72 hours after moxonidine administration, and compared with that before administration or the effect of saline. The experiment of depressurization with consecutively oral administration once a day. The grouping was the same to above-mentioned. Successive administration was for 7 days, once a day. The blood pressure and heart rate were determined at 1 hour before and after administration, and observed for 3 days after drug withdrawal. Recommended dose of moxonidine hydrochloride for human was about 0.4 mg/kg, while the oral dose for rats were around 0.04 mg/kg based on the animals' surface area. ②Changes of blood pressure and heart rate in anaesthesia rats with renal hypertension with a catheter on the carotid artery directly: 0.2 mg/kg drug liquor was given with gastric perfusion. The rats were randomly divided into 5 groups with 10 ones in each group: 0.13,0.4, 1.3 mg/kg moxonidine hydrochloride groups, 0.13 mg/kg clonidine hydrochloride group and saline control group. Mean arterial pressure was determined before and after administration at different time.MAIN OUTCOME MEASURES: Changes of blood pressure and heart rate in conscious and anesthetized renal-hypertensive rats before and after being administrated orally once or consecutively.RESULTS: All the rats were involved in the result analysis, without drop out during the trial. ①Moxonidine showed a dose-dependent effect on depressurization and descent of heart rate after once large dose oral administration in conscious renal-hypertensive rats. The 10-fold higher doses of moxonidine caused the same effects of clonidine. The decreasing of heart rate was little and short after consecutively small dose of oral administration of moxonidine, and which was similar to clonidine in percentage of depressurization. ②In anesthetized renal-hypertensive rats, moxonidine showed a dose-dependent effect on depressurization after once oral administration. There was no significant difference between moxonidine and clonidine in percentage of depressurization after 3 to 10-fold higher dose administration (P ＞ 0.05).CONCLUSION: The once higher dose oral administration of moxonidine has dose-dependent effect on depressurization for renal-hypertensive awake rats. Anesthesia. The effect of 10-fold dosage of moxonidine is equal to that of clonidine. The effect of 3-10-fold dosage of moxonidine is equal to that of clonidine in anesthesia renal-hypertensive rats. The small dose oral administration continuously of both moxonidine and clonidine with the same volume has the same depressurization effect in renal-hypertensive rats.

OBJECTIVE:To compare therapeutic efficacy and safety of desflurane and sevoflurane for anesthesia of elderly pa-tients. METHODS:A randomized,parallel-controlled method was used,120 elderly patients undergoing anesthesia were random-ized into trial group and control group,with 60 cases in each group. Based on routine medication and disposal,control group was additionally given sevoflurane for anesthesia,and trial group given desflurane for anesthesia. The blood pressure and heart rate were recorded before anesthesia,2 min and 10 min after intubation,2 min and 30 min after the start of the operation;the extubation time,eye opening time,consciousness recovery time and postoperative complications were also recorded. RESULTS:10 min after intubation,the heart rate of trial group was significantly faster then that of control group,with statistical significance(P<0.05);the blood pressure and heart rate of 2 groups had no statistically significant difference between 2 groups at other time points(P>0.05). The extubation time and consciousness recovery time of trial group were significantly shorter than those of control group, with statistical significance(P<0.05). The postoperative complications mainly were hypopiesia,hypertension,bronchospasm,nau-sea and vomiting,and dysphoria;the incidence of postoperative complications in trial group and control group were 25.0% and 40.0%,with statistical significance (P<0.05). CONCLUSIONS:Desflurane is effective with less postoperative complications for the anesthesia of elderly patients.

Chronic activation of the B-cell antigen receptor (BCR) signaling pathway performs a critical function in the pathogenesis of numerous subtypes of B-cell malignancies and transforms normal cells into malignant cells. Bruton tyrosine kinase (BTK) is a member of the TEC family of tyrosine kinases and is a key regulator of the BCR signaling pathway. BTK inhibition has emerged as a new target for therapeutic intervention in B-cell malignancies. This review summarizes recent developments of BTK inhibitors in B-cell malignan-cies.

This paper dealt with the influences of different media, temperature, light and pH to the amount of oospore formation. The results showed that millet agar, bai yun beau agar and soybean agar provided the favorable condition for oospore formation, the number of oospores was 131.6~149.6 /cm2, but a few oospores was formation on frozen pea agar. The results also suggested that the optimal conditions for oospore formation were 18℃, pH7 and darkness. The oospores failed to be formed tinder fluorescent or black light.

This study was aimed to investigate short-term modulation mechanism of scalp acupuncture according to the experimentation of cortical excitability and motor-related cortical potentials in ten normal volunteers.Through scalp needle stimulation in specific theoretical areas related to underlying cerebral surfaces,scalp acupuncture can be used as an effective therapy by changes in relative cerebral function,which is the basic principle.According to the available neurophysiological data in our study,the modulation of scalp acupuncture displayed in late part of motor-related cortical potential noticed mainly in the motor and sensory cortexes,contralateral to the needle stimulate site.Also,acupuncture can develop inhibition at longer interstimulus intervals,in paired pulse magnetic stimulation,from the somatosensory input of contralateral sensory cortex via scalp needle stimulation.In our study,it might be difficult to produce more acupuncture inhibition.The maintaining of scalp needles subcutaneously without other management after achieving needling sensations cannot achieve the requested strength of the acupuncture stimulation.

Objective:To investigate the effect of saw palmetto extract ( SR ) on GL261 glioma in rats and immune system.Methods:The 40 rats were divided into 4 groups randomly,one was the control group without tumor( the blank group) ( n=10) ,the other 30 rats were given subcutaneous inoculation of tumor cells and then divided into 3 groups:the tumor-bearing group( n=10) ,low dose SR group( n=10) ,and high dose SR group( n=10).After 1 week fed,the rats of SR groups were given the saw palmetto extract,low dose group 50 mg/kg once a day every other day and 300 mg/kg of high dose group every other day.The tumor-bearing groups received the same dose of distilled water.After 4 weeks fed, we measured the tumor weight and the inhibition rate was calculated.The tumor cell apoptosis was detected by TUNEL staining.The activity of splenic macrophages was detected by macrophage phagocytosis of chicken erythrocytes and CD4+lymphocytes by flow cytometry.Results:①The SR groups compared with tumor group, the tumor weight was significantly reduced(F=62.678,P=0.000).The tumor inhibition rate was significantly higher in high dose group.②The apoptosis of tumor cells in tumor-bearing group was significantly less than SR groups and the apoptosis was significantly increased after treatment with SR ,especially in high dose SR group(F=1.287,P=0.000).③Compared with the blank group,the macrophage activity decreased remarkablely.SR enhanced the macrophage activity obviously, positively related to doses of SR ( F=141.205,P=0.000;F=126.903,P=0.000).④In the tumor-bearing group,the CD4+lymphocyte count decreased significantly.After SR treatment,the CD4+lymphocyte increased and were positively related to the dose of SR.Conclusion: Saw palmetto extract can strengthen the immune system.

OBJECTIVE:To observe the efficacy and safety of low-dose ropivacaine combined with sufentanil in the upper limb anesthesia. METHODS:100 patients with upper limb anesthesia were randomly divided into control group and test group. Control group was intravenously injected 20 ml 0.5% ropivacaine;test group was intravenously injected 20 ml 0.375% ropivacaine +10 μg sufentanil. The anesthesia effect,disappearance time of pain,duration of analgesia,and pain visual analogue (VAS) score before anesthesia(T0),5 min(T1),15 min(T2)and 30 min(T3)after anesthetic block,and 4 h(T4)and 8 h(T5)after surgery,and in-cidence of adverse reactions in 2 groups were observed. RESULTS:VAS scores at T1-T5 in test group and T2-T4 in control group were significantly lower than T0,test group was lower than control group at T1,T2 and T5,the differences were statistically signifi-cant(P<0.05). The total effective rate of anesthesia in test group was significantly higher than control group,disappearance time of pain was significantly shorter than control group,duration of analgesia was significantly longer than control group,incidence of adverse reactions was significantly lower than control group,the differences were statistically significant(P<0.05). CONCLU-SIONS:Both the efficacy and safety of low-dose ropivacaine combined with sufentanil in the upper limb anesthesia are good.

Objective To compare and analyze the differences and characteristics of three strain mouse models in-fected by influenza virus aerosol inhalation, and provide the reference for choosing the appropriate infection model in the re-search of pathogenesis of influenza and the development of vaccines and drugs.Method C57BL/6, BALB/c and ICR mice were infected with A/Puerto Rico/8/34 (H1N1) virus strain by aerosol inhalation.The symptoms and body weight of mice were observed every day.At 3, 7, 14 days after infection, the mice were sacrificed.The lungs of mice were weighed, then virus assay and pathological observation were carried out.Results The three strains of mice were infected.The sur-vival rate in the C57BL/6 mice was lower than those in the BALB/c and ICR mice.The lung index and viral load of C57BL/6 mice were significantly higher than those of ICR mice ( P<0.05) at 3 days after infection.The pathological changes of C57BL/6 mice were also more obvious than other two strains.Compared with other two mouse strains, the weight recovery of BALB/c mice was the slowest.The survival rate in BALB/c mice was higher than that of C57BL/6 mice and lower than that of ICR mice.The lung index and viral load were not significantly different among the three strains of in-fected mice.The pathological changes among the three strains of infected mice were similar, but the degrees of pathological changes in the BALB/c mice were milder than in the C57BL/6 mice and worse than in the ICR mice.Compared with other two mouse strains, the process of disease is similar, but the body weight, mortality, lung index, viral load, and the micro-scopic pathological changes were lighter in the ICR mice than in the other two strain mice.Conclusions The three strain mouse models can be established by influenza virus aerosol inhalation, but showing different characteristics.Appropriate strain mice can be chosen to build model according to different research purpose in the experiment.

Objective To investigate the change of transmembrane potential during Noradrenaline's early preconditioning phase and probe the mechanism of action.Methods Primary culture myocardial cell of neonate rat were divided into three groups at random.GroupⅠ is control group,Group Ⅱis treatment group by hypoxia,Group Ⅲ is treatment group by NA.Group Ⅰ didn't give any drug to culture for 40minutes;Group Ⅱ treated for 40minutes by hypoxia,Group Ⅲ exposed to Noradrenaline(0.2?g/ml)for 10 minutes then treated with hypoxia for 40 minutes.The fluorescence intensity of rhodamine-123 marked mitochondria were detected by flow cytometry.Results The fluorescence intensity of three Group were test by analysis of variance(F=461.3,P

The 54th ASH annual meeting should be considered as a revolution for treatment of lymphoma.Keep away from chemotherapy will not be just a dream,it based on the deep understand of lymphoma genesis and quick development of different targeted agents.The typical examples are the dramatic novel agents targeting B cell receptor signaling pathway and microenvironment.There combination,such as R-square regimen,showed unbelievable efficacy in indolent B cell lymphomas and tiny side effects.It is sure that therapy breakthroughs in aggressive B cell lymphomas and T/NK cell lymphomas are in the very near future.