?-GT activity and its substrate GSH content in tissue homogenate, epithe-lium and stroma of normal and benign hyperplastic human prostates were measured tostudy their relationship with pathogenesis of benign prostatic hyperplasia (BPH). Theresults were: a) ?-GT activity in BPH tissues were very much increased; but GSHcontent was much decreased; b) The ?-GT activity in epithelium of both normal andBPH were much higher than that in relative stroma. However epithelium GSH contentwere lower than that of stroma. Thase results suggested that increand ?-GT activity anddecreased GSH content play an important role in pathogenesis of BPH.

OBJECTIVE:To discuss the general rule on checking and acceptance of drug warehousing of prepared slices of Chinese crude drugs.METHODS:The warehousing acceptance data of prepared slices of Chinese crude drugs during 2003-2007 in our hospital were sorted and the reasons for drug acceptance rejection were analyzed.RESULTS:Of the 5 228 batches of prepared slices of Chinese crude drugs checked before acceptance over 5 years,138(2.61%) were unacceptable.Most of the rejected batches were prepared processing slices of Chinese crude drugs.There were more than 30 factors resulted in the rejection.CONCLUSION:The key to ensure the quality of prepared slices of Chinese crude drugs is to improve their processing level.

Prostatic stromal fibroblasts were obtained from human fetal prostate and the effect of androgen (DHT) , estrogen (E2) , and human prostatic growth factor (hPGF) on the proliferation of the cultured fibroblasts was studied. The results showed that: 1) DHT and hPGF had significant stimulatory effect on proliferation of the cells; DHT had a delayed stimulatory effect, while hPGF had an immediate one. 2) E2 had no effect on proliferation of the cultured fibroblasts. 3) Used jointly, E2 and DHT had no stimulating effect on the proliferation of the fibroblasts. The results suggest that the effect of DHT on the proliferation of human prostatic stromal fibroblasts may be indirect. It possibly regulates the growth of prostate by stimulating autocrine or paracrine function of the prostatic cells.

Objective To investigate the application of get-well treatments in the suffers after thorax opera- tion over 70 years old. Methods 68 suffers after thorax operation over 70 years old were divided to two groups. Comparison group( n = 32 ) is actualized general nurse. Experiment group( n = 36) is actualized lung function exercise before operation, and get-well treatments after operation, such as psychologic get-well, deep breath, abdominal breath, effective cough and sputuming, applying apparatus training breath; exercise limbs and body, atomization in- breath, and so on. Results The suffers in experiment group who have lung atrophy,lung infection, liquid in the tho- rax,the time in the hospital and the time holding thorax pipe are less than the suffers in comparison group. Conclu- sion Lung function exercise before operation and effective get-well treatments after operation have significant ef- fects on old suffers.

Objective To explore the effect of collagen membrane combined with recombinant human bone morphogenetic protein-2(rhBMP-2) on periodontal tissue regeneration and repair in periodontal defect models of rats.Methods Eighteen male healthy Wistar rats,which were made experimental periodontal bone defects in the inferior incisors,were randomly divided into three groups:control group,collagen membrane group(Co),collagen membrane and rhBMP-2 group(Co/rhBMP-2).Two rats from each group were sacrificed at 4,6,8 weeks after operation.The mandibles were removed,and examined under light microscope.Results In Co/rhBMP-2 group: 4 weeks after operation,a large amount of bone formed in the defects;6 weeks later,new bone filled in the defects;8 weeks later,alveolar bone emerged,newly-formed periodontal ligament and cementum localized at the root edges,no gingival epithelium was observed.In Co group:4 weeks after operation,new bone formed at the edge of the defects;6 weeks later,a larger amount of bone and periost were observed;8 weeks later,a small amount of periodontal ligament and cementum localized at the root edges.In control group: 4 weeks after operation,there was a small amount of newly-formed bone at the edge of the defects;6 weeks later,collagen fibers were found at the edge of defects;8 weeks later,a small amount of periodontal tissue reached its original height and gingival epithelium proliferated obviously.Conclusion Collagen membrane combined with rhBMP-2 which has not only conductive effect but also effect of osteoinduction,can prevent the long-epithelium growing,maintain the growth gap,and promote periodontal tissue to regenerate.

The 54th ASH annual meeting should be considered as a revolution for treatment of lymphoma.Keep away from chemotherapy will not be just a dream,it based on the deep understand of lymphoma genesis and quick development of different targeted agents.The typical examples are the dramatic novel agents targeting B cell receptor signaling pathway and microenvironment.There combination,such as R-square regimen,showed unbelievable efficacy in indolent B cell lymphomas and tiny side effects.It is sure that therapy breakthroughs in aggressive B cell lymphomas and T/NK cell lymphomas are in the very near future.

Objective To assess the clinical effects and adverse reactions of different regimens of chemotherapies for elderly patients with advanced gastric cancer. Methods 95 elderly patients with advanced gastric cancer were randomly divided into 3 groups. The regimens of OLF,OX and HCF were used to treat 38 patients,25 patients and 32 patients respectively. The short-term curative effects and side effects on tumor were gradeded according to the criteria formulated by WHO. The curative effects and adverse reactions were assessed and the time of disease progression was calculated at least 2 cycles after chemotherapy. Results The efficacy rate was 55.26%, 60. 00% and 53. 13% respectively,and statistical analysis showed no significant difference(P ＞ 0.05). The time of disease progress was 23,30 and 24 weeks respectively, there were significant differences (P ＜ 0.05). The major side effect were neutropenia, gastrointestinal reactions, and were able to be tolerated without a chemotherapy-related death. Conclusion Three regimens of chemotherapies had better curative effects and mild side effects for elderly patients with advanced gastric cancer,and could improve the quality of life of the patients,and are worth of clinical promotion.

This study was aimed to investigate short-term modulation mechanism of scalp acupuncture according to the experimentation of cortical excitability and motor-related cortical potentials in ten normal volunteers.Through scalp needle stimulation in specific theoretical areas related to underlying cerebral surfaces,scalp acupuncture can be used as an effective therapy by changes in relative cerebral function,which is the basic principle.According to the available neurophysiological data in our study,the modulation of scalp acupuncture displayed in late part of motor-related cortical potential noticed mainly in the motor and sensory cortexes,contralateral to the needle stimulate site.Also,acupuncture can develop inhibition at longer interstimulus intervals,in paired pulse magnetic stimulation,from the somatosensory input of contralateral sensory cortex via scalp needle stimulation.In our study,it might be difficult to produce more acupuncture inhibition.The maintaining of scalp needles subcutaneously without other management after achieving needling sensations cannot achieve the requested strength of the acupuncture stimulation.

Objective To study GAP\|43mRNA expression during nerve injure and regeneration. Methods Rat sciatic nerve was crushed then, in situ hybridization technique was used to explore GAP\|43mRNA expressions in spinal cord and dorsal root ganglion (DRG). Results The neurons of spinal cord and DRG were detected to have GAP\|43 hybridization sinal by 2 days after sciatic nerve lesion. At later times(4,7 and 14 days postsurgery) the anterior horn motor neurons and DRG cells showed an increase in the number of GAP\|43mRNA positive neurons, followed by a significant rise in their content of GAP\|43mRNA. The number of GAP\|43mRNA positive neurons was decreased by 30 days postinjure, and was nearly nomal 60 days postinjure.Conclusion GAP\|43mRNA expression was increased during peripheral nerve injure and regeneration. The study showed that GAP\|43 may play a key role in nerve regeneration. [

Objective To discuss the importance of regulatory role of panaxoside Rg1 in Cdk5 in the process of hippocampal neuron radioactive damage protection.Methods Radioactive brain damage in vivo 40 models were built,and divided into 4 groups,including 0 Gy group (short for blank group),pure panaxoside Rg1 preconditioning group (short for control group),30 Gy group (short for model group),and 30Gy + panaxoside Rg1 preconditioning group (short for traditional Chinese medicine group).Hippocampal neurons were separated and trained.Hippocampal neuron apoptosis condition was tested in every group by 4′,6-diamidino2-phenylindole (DAPI) staining method.The p35 and p25 protein expressions were tested with Western blot.Cdk5 was restrained by Cdk5 restrainer roscovitine.Hippocampal neuron damage after Cdk5 blocking-up was observed with changes of X ray in every group.Results Compared with blank group,no significant difference was found in nuclear shrinkage percentage,the number of neuron survival,and the protein expression levels of p35 and p25 in control group ; nuclear shrinkage percentage and the protein expression levels of p35 and p25 were significantly increased and the number of neuron survival was significantly decreased in the model group and traditional Chinese medicine group (P ＜ 0.05).Compared with model group,nuclear shrinkage percentage and the protein expression levels of p35 and p25 were significantly decreased and the number of neuron survival was significantly increased in the traditional Chinese medicine group (P ＜ 0.05).For the addition of dimethyl sulfoxide (DMSO) in every group,the nuclear shrinkage percentage was not significantly changed in control group compared with blank group,was significantly increased in model group and traditional Chinese medicine group compared with blank group (P ＜ 0.05),and was significantly decreased in traditional Chinese medicine group compared with model group (P ＜ 0.05).Conclusions Panaxoside Rg1 can reduce neuron apoptosis by controlling Cdk5,and plays a protective role in hippocampal neuron radioactive damage.