1.Study on the standardization of traditional Chinese medical literature classification
Xiaomei KANG ; Qing DUAN ; Yuqin BAO
International Journal of Traditional Chinese Medicine 2010;32(4):345-346
In this paper, the history, problems being encountered in current situation and significance of carrying out standardization of TCM literature classification were discussed.
2.Study on construction of public management system of traditional Chinese medical picture resources
Deli YANG ; Deying CAI ; Li KANG ; Xiaomei KANG ; Yuqin BAO
International Journal of Traditional Chinese Medicine 2010;32(1):72-73
The author made a feasible study on the construction of public management system of traditional Chinese medical picture resources based on current existence of TCM pictures.Pictorial resources of original separation,multielement,isomerism,and scattered distribution,were united by physical and logical patterns for the purpose of enhancing their availability.Public management system plat Of TCM picture resources was set up,with the characteristics of practicability,novelty,originality,and suitability for scientific research,teaching and public propagate education.
3.Synergistic prohibitory effect of docosahexaenoic acid pins fluorouracil on human gastric cancer cell SGC7901
Quan WU ; Jianchun YU ; Yuqin LIU ; Weiming KANG ; Weidong GUO
Chinese Journal of Clinical Nutrition 2009;17(4):210-214,illust 1
U plus DHA.
4.Effect of pentoxifylline on proliferation of human keloid fibroblasts
Hongxia FENG ; Yan XIN ; Yuqin HAO ; Chunyi KANG
Chinese Journal of Tissue Engineering Research 2010;14(11):1923-1926
BACKGROUND:In recent years,pentoxifyIline has been found to have a wide range of anti-fibrosis capacity However,there are few studies explore the suppress effect of pentoxifyIline on fibroblasts in human keloid.and the maximum inhibitory concentration remains poorly understood.OBJECTIVE:To study the effect of pentoxifyIline on proliferation activity of human keloid fibroblasts and to select the maximum inhibitory concentrationMETHODS:Human keloid fibroblasts were used as original cells,passaged till the 5~(th) to the 8~(th) generations.and then divided into the experimental and control groups.PentoxifyIline with concentrations of 0.1,0.25,0.5,1.0,2.0 and 3.0 g/L were added to the experimental group.The effects of different concentrations of pentoxifylline on proliferation of keloid fibroblasts were detected by MTT chromometry.RESULT AND CONCLUSION:Compared with the control group,the inhibitory effect of pentoxifylline on the proliferation of keloid fibroblasts was more evident in the experimental group(P<0.05)The inhibition rates of pentoxifylline on proliferation of keloid fibroblasts showed apparently time-and dose-effect relationships within the concentration of 0.1-2.0 g/L.which presented a greatest level at 96 hours after culture.The maximum inhibitory rate was 53 37%,and the concentration was 2.0 g/L in the experimental group.Consequently,pentoxifyIline plays a notable inhibitory role in the proliferation of human keloid fibroblasts with concentration of 2.0 g/L at 96 hours after culture.
5.Analysis of the relationship between solar greenhouse operation and occupational high incidence diseases
Wu LIU ; Jiusheng WEI ; Zhenxia KOU ; Yuqin KANG ; Xiaoyan CHE
Chinese Journal of Industrial Hygiene and Occupational Diseases 2024;42(7):504-509
Objective:To understand the health status of solar greenhouse workers, to provide scientific basis for the development of occupational high incidence diseases prevention and control strategies.Methods:In July 2019, a random cluster sampling method was used to select 245 workers engaged in solar greenhouse vegetable cultivation in Daba Village, Jingyuan County, Baiyin City, Gansu Province as the solar greenhouse operation group. Matched by gender, age, marital status, body mass index (BMI), 282 people from adjacent Shaliang Village who did not engage in solar greenhouse operation were selected as the control group. Field investigation and health examination were carried out among the study subjects. The general situation, facial features examination results, ophthalmic examination results, bone and joint examination results and skin examination results were compared between the two groups. And the multiple logistic regression analysis was used to analyze the influencing factors of abnormal bone and joint examination (upper limbs, lower limbs, hands and spine bone joints) in study subjects.Results:There were statistically significant differences in smoking age and alcohol consumption between the two groups ( P<0.001). Compared with the control group, the abnormal detection rates of nose examination, throat examination, slit lamp examination, conjunctival examination, lower limb bone joint examination, hand bone joint examination, spine examination, head and neck skin examination, trunk skin examination, upper limb skin examination, and lower limb skin examination in the solar greenhouse operation group were higher, and the differences were statistically significant (χ 2=11.53, 7.94, 9.92, 27.93, 79.32, 81.42, 9.43, 6.79, 9.76, 4.34, 8.29, P<0.05). Multivariate logistic regression analysis showed that after adjusting for gender, age, marital status, education level, BMI, compared with the control group, solar greenhouse operation was a risk factor for abnormal bone and joint examination ( OR=1.178, 95% CI: 1.151-2.143, P=0.001) . Conclusion:Solar greenhouse operation has a certain harmful effect on health of workers, and solar greenhouse workers have an increased risk of abnormal diseases of upper limbs, lower limbs, hands and spine bone joints.
6.Analysis of the relationship between solar greenhouse operation and occupational high incidence diseases
Wu LIU ; Jiusheng WEI ; Zhenxia KOU ; Yuqin KANG ; Xiaoyan CHE
Chinese Journal of Industrial Hygiene and Occupational Diseases 2024;42(7):504-509
Objective:To understand the health status of solar greenhouse workers, to provide scientific basis for the development of occupational high incidence diseases prevention and control strategies.Methods:In July 2019, a random cluster sampling method was used to select 245 workers engaged in solar greenhouse vegetable cultivation in Daba Village, Jingyuan County, Baiyin City, Gansu Province as the solar greenhouse operation group. Matched by gender, age, marital status, body mass index (BMI), 282 people from adjacent Shaliang Village who did not engage in solar greenhouse operation were selected as the control group. Field investigation and health examination were carried out among the study subjects. The general situation, facial features examination results, ophthalmic examination results, bone and joint examination results and skin examination results were compared between the two groups. And the multiple logistic regression analysis was used to analyze the influencing factors of abnormal bone and joint examination (upper limbs, lower limbs, hands and spine bone joints) in study subjects.Results:There were statistically significant differences in smoking age and alcohol consumption between the two groups ( P<0.001). Compared with the control group, the abnormal detection rates of nose examination, throat examination, slit lamp examination, conjunctival examination, lower limb bone joint examination, hand bone joint examination, spine examination, head and neck skin examination, trunk skin examination, upper limb skin examination, and lower limb skin examination in the solar greenhouse operation group were higher, and the differences were statistically significant (χ 2=11.53, 7.94, 9.92, 27.93, 79.32, 81.42, 9.43, 6.79, 9.76, 4.34, 8.29, P<0.05). Multivariate logistic regression analysis showed that after adjusting for gender, age, marital status, education level, BMI, compared with the control group, solar greenhouse operation was a risk factor for abnormal bone and joint examination ( OR=1.178, 95% CI: 1.151-2.143, P=0.001) . Conclusion:Solar greenhouse operation has a certain harmful effect on health of workers, and solar greenhouse workers have an increased risk of abnormal diseases of upper limbs, lower limbs, hands and spine bone joints.
7.Overexpression of long noncoding RNA HMMR-AS1 promotes progression of lung adenocarcinoma
Yongjing ZENG ; Tao XU ; Kang LIN ; Bangshun HE ; Yuqin PAN ; Huiling SUN ; Shukui WANG
Chinese Journal of Clinical Laboratory Science 2019;37(5):389-395
Objective:
The purpose of this study is to explore the biological function of long non-coding RNA (lncRNA) HMMR-AS1 in proliferation and metastasis of lung adenocarcinoma (LUAD).
Methods:
Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of HMMR-AS1 and its sense strand HMMR in LUAD cell lines. Then we knock down the HMMR-AS1 expression through small interfering RNA and evaluate the transfection efficiency and its effect on the expression of HMMR. CCK-8 (cell counting kit), clone formation, flow cytometric analysis, wound scratch assay and transwell assay were used to assess the biological function of A549 and H1299 cells. Western blot was used to detect the protein expression of HMMR in the two cell lines after transfection with si-HMMR-AS1.
Results:
The expression of HMMR-AS1 in A549 and H1299 cells of LUAD cell line was markedly higher than that in normal lung epithelial cell BEAS-2A by upregulating approximately 3.06 and 5.02 folds (P<0.05), respectively. After transfection with si-HMMR-AS1, the expression of HMMR-AS1 markedly reduced in both levels of transcription and protein (P<0.05). Furthermore, knocking down of HMMR-AS1 significantly inhibited the proliferation, migration and invasion abilities, and increased the apoptosis rates of A549 and H1299 cells.
Conclusion
LncRNA HMMR-AS1 could promote malignant progression of LUAD cells through enhancing the growth, migration and invasion ability of LUAD cells.