Objective To study the effectiveness of laparoscopic radiofrequency ablation in the application of primary liver cancer.Methods 92 patients with primary liver cancer were randomly divided into the two groups,the control group(n=46)and the observation group(n=46).The control group was operated through hepatectomy,while the observation group was operated through laparoscopic radiofrequency ablation.The recurrence rate,survival time, quality of life in the two groups were observed.Results Separately on the recurrent tumor of different diameter were compared between the two groups,no significant difference in relapse rate(χ2 =2.14,1.92,all P>0.05).There was no significant difference between the two groups in 3,12,24 months about survival cases (χ2 =1.24,1.96,2.08,all P>0.05 ).To compute the expected survival rate with Kaplan Meier method,the control group survival time was 22.3 months,the observation group survival time was 21.1 months,no significant difference between the two groups (P>0.05 ).The two groups after operation in 3 months,the quality of life of patients all patients were increased,and the observation group improved significantly,compared with the control group (t=2.676,P<0.05 ).Conclusion Laparoscopic radiofrequency ablation had quite effect and liver resection operation curative,but can improve the quality of life.

Objective To investigate the change of interleukin-5(IL-5)level and mRNA expression in peripheral blood from asthma patients during paracmasis before and after administration of taurine,and explore its clinical significance.Methods A randomized,placebo control,double blind and prospective study was conduced in 84 patients with mild to moderate asthma During paracmasis.The patients were randomly divided into group A(taurine taken orally for one year from beginning of paracmasis)and group B(placebo taken orally for one year from beginning of paracmasis).The symptom score of asthma,lung function paramcter(FEVI),IL-5 level and mRNA expression in peripheral blood were measured and rccorded before and after treatment.The results were compared with those of healthy control group(group C,n=10).Results There were significant differences in the above indices between group A and group B:symptom score of asthma(12.5 + 4.8 vs 15.2±5.1);lung function parameter[FEV1(L)2.6±0.5 vs 2.2±0.8];IL-5 level(pg/mL)(16.3±4.5 vs 26.9 ±12.7);IL-5 mRNA(0.21 ± 0.09 vs 0.35 ± 0.14).The basic level and mRNA expression of IL-5 were significantly higher in group A and group B than that in group C.The serum level and mRNA expression of IL-5 were positively correlative with symptom score of asthma in group A(r=0.42,P＜0.05).Conclusion The exists ovcrexpression of IL-5 in asthma patients during paracmasis and IL-5 level reflects the scverity of pathogenetic condition.Therefore,IL-5 measurement may provide a good reference for clinical therapy of asthma.

AIM: To explore the effect of acute and chronic hypoxia on the chemotaxis of polymorphonuclear leukocytes(PMNs) of rats.METHODS: Serum and PMNs were separated from rats under normoxia, acute hypoxia and chronic hypoxia condition,and cell aspiration technology system was used to determine the chemotactic migration of pseudopod of PMNs induced by serum as chemotactic factor.RESULTS: Induced by autoserum,the length of pseudopod of PMNs migrated into micropipette [(1.200?0.178)?m,(1.094?0.164)?m ]under acute hypoxic and chronic hypoxic condition was significantly longer than the corresponding value for control[(0.914?0.156)?m,P

Objective:To establish a GC method for the determination of eugenol in Jingbutong Babugao. Methods: The determination was carried out on a PEG 20M capillary column with temperature at 160 ?C , FID detector with temperature at 230 ?C . Results: The eugenol sample showed a good linear relationship at the range of 0.38～6.14?g and correlation coefficient was 0.9993. The average recovery was 98.60% with a RSD of 2.18%. Conclusion: The method is simple, accurate, reproducible and can be used for determination of eugenol in Jingbutong Babugao.

Interleukin-6(IL-6)is associated with cancer staging, survival, apoptosis and the sensitivity to chemotherapeutic drugs, and it is overexpressed in the cancer tissue and serum of cancer patient.IL-6 Can regulate proliferation and differentiation of cancer cells through IL-6/STAT3 signaling pathway. Therefore, blocking IL-6/STAT3 signaling pathway has potential therapeutic implications for cancer.

BACKGROUND:The pain caused by osteoporosis and osteoporotic fracture is usually treated with non-steroidal anti-inflammatory drugs (NSAIDs) in clinic,which has been reported that dysfunction of liver and kidney will follow the use,but what has happened in the tissue of liver and kidney is not reported.OBJECTIVE:To study the effect of NSAIDs on the tissue of liver and kidney in osteoporotic fracture rats.DESIGN,TIME AND SETTING:Randomized controlled animal experiment.The experiment was completed in the Animal Experiment Center in Shanghai University of Traditional Chinese Medicine from August 2007 to February 2008.MATERIALS:A total of 24 female SD rats,8-month-old,weighing 300-320 g,were randomly divided into 3 groups,saline group,diclofenac sodium (fracture before medicine) group and diclofenac sodium (fracture after medicine) group,with 8 rats in each group.METHODS:The rats were housed for 3 months after ovariectomized to establish osteoporosis models.Rats in the saline group and diclofenac sodium (fracture before medicine) group were administrated saline and diclofenac sodium after transverse osteotomy at the middle shaft of bilateral femur respectively;in the diclofenac sodium (fracture after medicine) group were administrated 5 mg/(kg?d) diclofenac sodium for 3 weeks,then received transverse osteotomy at the middle shaft of bilateral femur.MAIN OUTCOME MEASURES:The histological observation of liver and kidney was performed at weeks 2,3,4 and 6 after fracture.RESULTS:In the diclofenac sodium (fracture before medicine) group,diclofenac sodium caused the inflammatory response at glomeruli,which exhibited expansion of tubular lumen,edema of epithelial cells,disappeared cell nuclei,degeneration and necrosis of renal tubule,cell debris and drugs crystals accumulated in the tubular lumen,congestion and inflammatory cell infiltration of renal interstitium.Administrating diclofenac sodium may cause the inflammatory response at portal area,indistinct structure of hepatic lobule,hepatic cells edema,steatosis and necrosis.Administrating diclofenac sodium before osteoporotic fracture also resulted in tissue lesions in kidney and liver,the damage would continue about 3 weeks.CONCLUSION:The diclofenac sodium cause histological lesions of kidney and liver in osteoporotic rats,especially in kidney.The histological lesions of kidney and liver are inreversible after administrating diclofenac sodium for a long time.

BACKGROUND: The pain caused by osteoporosis and osteoporotic fracture is usually treated with non-steroidal anti-inflammatory drugs (NSAIDs) in clinic, which has been reported that dysfunction of liver and kidney will follow the use, but what has happened in the tissue of liver and kidney is not reported. OBJECTIVE: To study the effect of NSAIDs on the tissue of liver and kidney in osteoporotic fracture rats. DESIGN, TIME AND SETTING: Randomized controlled animal experiment. The experiment was completed in the Animal Experiment Center in Shanghai University of Traditional Chinese Medicine from August 2007 to February 2008.MATERIALS: A total of 24 female SD rats, 8-month-old, weighing 300-320 g, were randomly divided into 3 groups, saline group,diclofenac sodium (fracture before medicine) group and diclofenac sodium (fracture after medicine) group, with 8 rats in each group.METHODS: The rats were housed for 3 months after ovariectomized to establish osteoporosis models. Rats in the saline group and diclofenac sodium (fracture before medicine) group were administrated saline and diclofenac sodium after transverse osteotomy at the middle shaft of bilateral femur respectively; in the diclofenac sodium (fracture after medicine) group were femur.MAIN OUTCOME MEASURES: The histological observation of liver and kidney was performed at weeks 2, 3, 4 and 6 after fracture.RESULTS: In the diclofenac sodium (fracture before medicine) group, diclofenec sodium caused the inflammatory response at glomeruli, which exhibited expansion of tubular lumen, edema of epithelial cells, disappeared cell nuclei, degeneration and necrosis of renal tubule, cell debris and drugs crystals accumulated in the tubular lumen, congestion and inflammatory cell infiltration of renal interstitium. Administrating diclofenac sodium may cause the inflammatory response at portal area, indistinct structure of hepatic Iobule, hepatic cells edema, steatosis and necrosis. Administrating diclofenac sodium before osteoporotic fracture also resulted in tissue lesions in kidney and liver, the damage would continue about 3 weeks.CONCLUSION: The diclofenac sodium cause histological lesions of kidney and liver in osteoporotic rats, especially in kidney. The histological lesions of kidney and liver are inreversible after administrating diclofenac sodium for a long time.

OBJECTIVEThis study aims to investigate the prevalence and distribution of teeth number and morphology abnormalities in Chinese pediatric patients' permanent teeth at the mixed dentition stage by performing panoramic radiographs analysis.

METHODSA total of 4 347 panoramic radiographs of pediatric patients aged 5 years to 15 years who were admitted to the hospitals from September 2011 to September 2013 were reviewed. The presence of teeth number and morphology abnormalities were recorded as follows: congenitally absent teeth, supernumerary teeth, odontoma, microdontia, fused teeth, dilaceations, and cusp deformity. SPSS 16.0 software was used for statistical analysis.

RESULTSThe prevalence of teeth number and morphology abnormalities in pediatric patients' permanent teeth was 31.79% (1,382/4,347). The prevalence was higher in males. Significant difference between the genders was observed (P < 0.05). Tooth agenesis, the most frequent dental anomaly (15.00%, 652/4,347), was considerably common in females; supernumerary teeth (12.61%, 548/4,347) followed, which was significantly common in males. Both differences were statistically significant (P < 0.05). The levels of prevalence of both microdontia and dilacerations were 4.00% (174/4,347) and 2.16% (94/4,347). The prevalence of cusp deformity, odontoma, and fused teeth were relatively low at 0.74% (32/4,347), 0.51% (22/4,347), and 0.39% (17/4,347), respectively.

CONCLUSIONThe prevalence of teeth number and morphology abnormalities in children's permanent teeth at the mixed dentition stage is apparently high. Tooth agenesis and supernumerary teeth are the most frequent. Panoramic radiograph is an efficient method for the early diagnosis of teeth number and morphology abnormalities. This method can assist pediatric dentists to formulate treatment plans for children at the appropriate time.

Adolescent ; Child ; Child, Preschool ; China ; epidemiology ; Dentists ; Dentition, Mixed ; Dentition, Permanent ; Female ; Fused Teeth ; epidemiology ; Humans ; Male ; Odontoma ; epidemiology ; Patient Care Planning ; Prevalence ; Radiography, Panoramic ; Sex Factors ; Tooth Abnormalities ; diagnostic imaging ; epidemiology ; Tooth, Supernumerary ; diagnostic imaging ; epidemiology

Objective:To study the effect of TNF-? on the activation and fungicidal activity of mouse peritoneal exudate cells.Methods:The mouse peritoneal exudate cells and macrophage were stimulated with IL-12、IL-18、IFN-?、TNF-? or monoclonal antibody.Cytokines and nitric oxide(NO) levels in the medium supernatant were determined with ELISA and Griess method respectively.Detected the numbers of viable Cryptococcus neoformans in macrophage.Results:Combined with IL-12 and IL-18 synergistically induced the production of TNF-? by peritoneal exudate cells and this effect can be inhibited by neutralizing anti-IFN-? mAb.Combined with IFN-? and TNF-? synergistically induced the production of NO by macrophage and potentiate its activity against Cryptococcus neoformans.Conclusion:The result suggests that the TNF-? plays an important role in enhancement of the fungicidal activity of macrophages. [