0.05), reduced obviously in hydrotherapy group as compared with sham group. Postsynaptic density (PSD) in hippocampus was more in intervention group than non-intervention group. Conclusion Enriched environment stimulus and hydrotherapy can promote the rehabilitation of neonatal rats with HIBD. The intervention effects of enriched environment and combined application of enriched environment stimulus and hydrotherapy is better than hydrotherapy. The change of NMDA receptor in hippocampus might be one of the mechanism.

OBJECTIVE:To observe clinical efficacy and safety of minocycline for acute retrograde pulpitis (ARP) and its ef fects on pain.METHODS:One hundred (100 teeth) ARP patients in Hospital of Qingdao Agriculture University and the Affiliated Hospital of Medical College during Jan.-Oct.in 2015 were divided into observation group and control group according to random number table,with 50 cases in each group (50 teeth).Control group was given root canal therapy of Formaldehyde cresol formocresol solution;observation group was given was given root canal therapy of Minocycline hydrochloride cream.Both groups were re checked 3 days later,and therapeutic efficacy was evaluated after 5 month follow-up;pain score,IL-2 and TNF-α levels were ob served before and 3 d after treatment,and the occurrence of ADR was recorded in 2 groups.RESULTS:The total response rate of observation group was 98.00％,which was significantly higher than 86.00％ of control group,with statistical significance (P＜ 0.05).There was no statistical significance in percussion pain score,mobility score,IL-2 and TNF-αt levels between 2 groups before treatment (P＞0.05).Three days after treatment,percussion pain score,mobility score,IL-2 and TNF-α levels of 2 groups were significantly decreased;the observation group was significantly lower than the control group,with statistical significance (P＜ 0.05).There was no statistical significance in the incidence of ADR between 2 groups (P＞ 0.05).CONCLUSIONS:Minocycline shows significantly therapeutic efficacy for ARP,and can effectively improve pain symptoms with good safety.

OBJECTIVE:To introduce the concept of evidence-based medicine and pharmacoeconomics to the pharmacists so as to promote rational use of drugs METHODS:The evidence-based medicine was described in detail concerning the fundamental,procedure,actions,limitations and directive function to hospital,and in comparison with the advanced level of pharmacoeconomics in foreign countries,the pharmacoeconomics in China was analyzed in respect of the developing history,research scope,personnel training,monograph and periodicals and the quantity and quality of research work RESULTS & CONCLUSION:The evidence-based medicine and pharmacoeconomics are still in starting period in China,however,after entering WTO,they have proved to be important day by day and will greatly improve the level of rational use of drugs in our country

Object To distinguish the genuine Dioscorea polystachya Turcz. from other species of Dioscorea L. such as D. persimilis Prain et Burkill, D. japonica Thunb., and D. alata L., conventionally used in some localities by sequencing their nuclear ribosomal RNA subunit (18S rRNA), to provide mole cular evidences for the quality control of drugs of Dioscorea L. Methods By direct PCR sequencing to detect the homology of 18S rRNA sequence of different species of Dioscorea L.. Results The 18S rRNA gene sequence of genuine D. polystachya, D. persimilis and D. japonica showed identical length of 1810 bp, whereas that of D. alata was 1807 bp. Multiple sequence alignment of 18S rRNA gene showed that the homology was identical between D.persimilis and the genuine D. polystachya; but a difference of 99.89% with D. japonica and 97.51% with D. alata. Conclusion DNA sequencing can provide an accurate and reliable mean for the identification of the origin and genuineness of Dioscorea L..

Approximately one-quarter of ischemic stroke occurred in vertebrobasilar artery system. Atherosclerotic vertebrobasilar artery stenosis is one of the major causes of posterior circulation stroke. In addition to the conventional medical and surgical treatment, endovascular intervention has received more and more attention, and it has become the most promising therapeutic approach.

OBJECTIVE:To introduce coordinate localization method as a new pharmaceutical care method to provide reference for rational use of drug in cancer patients. METHODS:The way that clinical pharmacists assisted physicians in prescribing for cancer patients with coordinate localization method was interpreted combining with examples. RESULTS:Coordinate localization method was conducive to rational use of drug in cancer patients and pharmacist’s self- quality construction. CONCLUSION:Coordinate localization method can effectively assist the doctor to prescribe rationally.

Systemic inflammatory response has a major role in the development and progression of cancer,and increased systemic inflammatory response usually correlates with poorer survival.Recently the evaluation index of systemic inflammatory response including neutrophil-lymphocyte ratio,C-reactive protein,glasgow prognostic score,platelet-lymphocyte ratio.Several previous studies show that these indexes are the independent prognostic factors for esophageal cancer.Research on the systemic inflammatory markers may also be valuable for the clinical treatment and prognosis of esophageal cancer.

AIM:To investigate the effect and the mechanism of tanshinone ⅡA in attenuating PM2.5-induced human umbilical vein endothelial EA.hy926 cell injury.METHODS:The samples of fine particulate matter (PM2.5) were collected in Guangzhou and made into suspension.Different concentrations (0, 20, 200 and 400 mg/L) of PM2.5 were added to EA.hy926 cells.The viability and apoptosis of EA.hy926 cells, the protein levels of p-p38 MAPK, Bax and Bcl-2 in the EA.hy926 cells, the contents of interleukin-6 (IL-6), tumor necrosis factor-α(TNF-α) and malonaldehyde ( MDA) , and the activity of superoxide dismutase ( SOD) and lactic dehydrogenase ( LDH) in the EA.hy926 cell culture supernatant were measured by MTT assay, flow cytometry, Western blot, ELISA and colorimetry, respectively.Tanshinone ⅡA at different concentrations (5, 10 and 20 μmol/L) or a specific inhibitor of p38 MAPK pathway, SB203580 (20μmol/L) , was added into the EA.hy926 cells to observe the effect of tanshinone ⅡA.RESULTS:Compared with control group, PM2.5 significantly increased the apoptosis, the contents of IL-6, TNF-αand MDA, the activity of LDH, and the protein levels of p-p38 MAPK and Bax/Bcl-2 ratio, but decreased the viability and SOD activity in the EA.hy926 cells (P<0.05).Compared with PM2.5 group, tanshinone IIA significantly decreased the apoptosis, the contents of IL-6, TNF-αand MDA, the activity of LDH, and the protein levels of p-38 MAPK and Bax/Bcl-2 ratio, but increased the viabil-ity and SOD activity in the EA.hy926 cells (P<0.05).CONCLUSION:Tanshinone ⅡA attenuates PM2.5-induced EA. hy926 cell injury via the inhibition of p38 MAPK pathway.

Objective To investigate the comparison of the clinical treatment efficacy of propafenone and amiodarone in patients with paroxysmal supraventricular tachycardia ( PSVT ) .Methods Retrospective a total of 89 patients with PSVT from June 2013 to June 2015 in our hospital were selected.According to the different administration methods, the patients were divided into the propafenone group of 46 cases and the amiodarone group of 43 cases.The changes of left ventricular end-systolic volume, left ventricular end-diastolic volume, ejection fraction, cardioversion success rate, mean cardioversion time, heart rate and side effects were compared.Results After treatment, the left ventricular end-systolic volume (52.31 ±8.34) mL/m2 , (54.28 ±7.23) mL/m2, left ventricular end-diastolic volume (47.31 ±8.56) mL/m2, (45.28 ±7.16) mL/m2 and ejection fraction (61.57 ± 0.76)%, (61.39 ±0.69)% were without significant differences.After treatment, the heart rates of the two groups were significantly decreased, the propafenone group ( 85.31 ±12.84 ) times/min, amiodarone group ( 87.26 ±11.95 ) times/min, and there were not significant differences.The recovery time of propafenone group was ( 19.34 ±4.76 ) , higher than the amiodarone group ( 25.69 ±6.09 ) ( P <0.05 ).But the success rate of cardioversion (52.17%) was lower than the amiodarone group (88.37%) (P<0.05).The side effect rates in the propafenone group (21.74%) was significantly higher than that in the amiodarone group ( 4.65%) ( P <0.05 ) .Conclusion Propafenone and amiodarone cardioversion PSVT have achieved good results, but propafenone rapid onset, the average cardioversion time is short, which is only suitable for acute tachycardia and without serious organic heart disease patients.Amiodarone onset is slower,but the cardioversion success rate is higher.

Objective To investigate the efficacy of tolterodine plus tamsulosin in rats with benign prostatic hyperplasia(BPH)induced low urinary tract syndrome(LUTS).Methods Male rats were castrated,then testosterone propionate were given by subcutaneous injection to induce BPH/LUTS model.Model rats were intragastrically administrated with 0.4mg/kg of tolterodine,0.04mg/kg of tamsulosin or(0.4+0.04)mg/kg of tolterodine plus tamsulosin for 14 days and urodynamics,residual urine volume and volume of bladder were mearsured.Results Tolterodine plus tamsulosin could simultaneously shorten the micturition duration,prolong the interval duration between micturition,decrease the threshold intravesical pressure at micturition and the max intravesical Pressure at micturition significantly.The combined-therapy method could also decrease the residual urine volume to a certein extent and had no obvious influence on enlarging the volume of bladder(P＜0.05 or P＜0.01).Conclusion Combination treatment with tolterodine and tamsulosin may efficiently ameliorate bladder outlet obstrution and overactive bladder simultaneously,with no extra danger of uroschesis.