OBJECTIVE:To observe clinical efficacy and safety of minocycline for acute retrograde pulpitis (ARP) and its ef fects on pain.METHODS:One hundred (100 teeth) ARP patients in Hospital of Qingdao Agriculture University and the Affiliated Hospital of Medical College during Jan.-Oct.in 2015 were divided into observation group and control group according to random number table,with 50 cases in each group (50 teeth).Control group was given root canal therapy of Formaldehyde cresol formocresol solution;observation group was given was given root canal therapy of Minocycline hydrochloride cream.Both groups were re checked 3 days later,and therapeutic efficacy was evaluated after 5 month follow-up;pain score,IL-2 and TNF-α levels were ob served before and 3 d after treatment,and the occurrence of ADR was recorded in 2 groups.RESULTS:The total response rate of observation group was 98.00％,which was significantly higher than 86.00％ of control group,with statistical significance (P＜ 0.05).There was no statistical significance in percussion pain score,mobility score,IL-2 and TNF-αt levels between 2 groups before treatment (P＞0.05).Three days after treatment,percussion pain score,mobility score,IL-2 and TNF-α levels of 2 groups were significantly decreased;the observation group was significantly lower than the control group,with statistical significance (P＜ 0.05).There was no statistical significance in the incidence of ADR between 2 groups (P＞ 0.05).CONCLUSIONS:Minocycline shows significantly therapeutic efficacy for ARP,and can effectively improve pain symptoms with good safety.

OBJECTIVE:To introduce the concept of evidence-based medicine and pharmacoeconomics to the pharmacists so as to promote rational use of drugs METHODS:The evidence-based medicine was described in detail concerning the fundamental,procedure,actions,limitations and directive function to hospital,and in comparison with the advanced level of pharmacoeconomics in foreign countries,the pharmacoeconomics in China was analyzed in respect of the developing history,research scope,personnel training,monograph and periodicals and the quantity and quality of research work RESULTS & CONCLUSION:The evidence-based medicine and pharmacoeconomics are still in starting period in China,however,after entering WTO,they have proved to be important day by day and will greatly improve the level of rational use of drugs in our country

0.05), reduced obviously in hydrotherapy group as compared with sham group. Postsynaptic density (PSD) in hippocampus was more in intervention group than non-intervention group. Conclusion Enriched environment stimulus and hydrotherapy can promote the rehabilitation of neonatal rats with HIBD. The intervention effects of enriched environment and combined application of enriched environment stimulus and hydrotherapy is better than hydrotherapy. The change of NMDA receptor in hippocampus might be one of the mechanism.

Objective To observe the effect of treating chronic renal failure with self-made Huazhuo Baoyuan decoction.Methods 90 patients of chronic renal failure belonging to spleen and kidney yang deficiency and turbid toxic obstruction type diagnosed by TCM syndrome differentiation were randomly divided into a treatment group and a control group according to the date of reception.40 cases in the control group were treated with conventional western medicine,which were low-salt high-quality low-protein diet; to correct acid-base balance and electrolyte imbalance; to correct anemia; adding amino acids; to control blood sugar in patients with history of diabetes; to control blood pressure in patients with hypertension.50 patients in the treatment group were additionally treated with self-made Huazhuo Baoyuan decoction on the basis of the control group.Such indexes as symptom score,serum creatinine (Scr),blood urea nitrogen (BUN),endogenous creatinine clearance rate (Ccr),hemoglobin (Hb),liver function:aspartate aminotransferase (AST),alanine aminotransferase (ALT) ; lipids:total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol (LDL-C) were observed before and after the treatment.Results After treatment,total effective rate was 88.0％ and 67.5％ in the treatment group and the control group respectively,the difference was statistically significant (P=0.012).The value of Scr (292.95± 85.95) μmol/L,BUN (22.69 ± 9.84) mmol/L,Ccr (22.69 ±9.84)rn1/min,CH(6.23 ± 0.57)mmol/L,TG(1.70± 0.22)mmol/L,HDL-C(1.45 ± 0.04)mmol/L,LDL-C(3.30 ±0.60) mmol/L showed significant difference after the treatment than before in the treatment group (P＜0.05 or P＜ 0.01).Compared with the control group of after treatment [Scr (336.63 ± 90.77)μmol/L,BUN(10.47 ±2.71) mmol/L,Ccr (19.47 ± 6.71) rnl/min,CH (7.21 ± 0.62) mmol/L,TG (2.30 ± 0.27) mmol/L,HDL-C (0.87 ±0.05)mmol/L,LDL-C (5.60±0.70)mmol/L],the difference was statistically significant(P＜0.05).Conclusion Self-made Huazhuo Baoyuan decoction was effective and safe to treat chronic renal failure.

ObjectiveTo observe the effects of Shengqing-Jiangzhuo decoction combined with western medicine on metabolic syndrome.Methods120 patients with metabolic syndrome in Gaomi City People's Hospital of Traditional Chinese Medicine from March 2008 to July 2011 were randomly divided into a treatment group and a control group according to case number,with 60 cases in each group.The control group was treated with Western medicine individualized and symptomatic treatment,while on the basis of the control group the treatment group was additionally treated with Shengqing-Jiangzhuo decoction.Both groups were continuously treated for 2 months.The main symptoms of the two groups of patients before and after clinical efficacy as well as blood pressure,body mass index (BMI),waist-hip ratio (WHR) lipid levels,glycosylated hemoglobin (HbA1C) changes were observed.Results① The clinical efficacy:After treatment,dizziness,dry mouth,constipation,bloating,irritability and other symptoms improved significantly,with 85.00％ and 73.33％ of the total effective rate in treatment group and control group respectively; ② laboratory parameters detection:there was statistical difference between the control group and the treatment group in terms of systolic blood pressure (127.30±8.00) mm Hg,diastolic blood pressure (81.35±3.81) mm Hg,body mass index (23.3±1.48),waist-hip ratio (0.85 ± 0.07),as well as cholesterol (3.32 ± 0.81)mmol/L triglyceride (1.76 ±1.01) mmol/L,low-density lipoprotein cholesterol (2.20±0.72)mmol/L,high density lipoprotein cholesterol (1.39±0.14) mmol/L,glycosylated hemoglobin (6.58±0.82) mg/dl,(P＜0.05 or P＜0.01).Conclusion Shengqing-Jiangzhuo decoction significantly improved the clinical efficacy of metabolic syndrome.

Systemic inflammatory response has a major role in the development and progression of cancer,and increased systemic inflammatory response usually correlates with poorer survival.Recently the evaluation index of systemic inflammatory response including neutrophil-lymphocyte ratio,C-reactive protein,glasgow prognostic score,platelet-lymphocyte ratio.Several previous studies show that these indexes are the independent prognostic factors for esophageal cancer.Research on the systemic inflammatory markers may also be valuable for the clinical treatment and prognosis of esophageal cancer.

Gastrointestinal stromal tumor is one kind of the most common gastrointestinal tumors derived from the mesenchymal tissue.In most cases,it occurs in adults,while rarely occurs in adolescents.The clinical diagnosis of pediatric GIST can refer to the diagnosis standard of adults GIST,but it pays more attention to genetic diagnosis.The main treatment for pediatric GIST is surgery,but for advanced or unresectable patients,we can adopt imatinib or sutent for targeted therapy according to the results of genetic testing.

OBJECTIVE:To study the inhibitory effects of berberine on EA.hy926 human umbilical vein endothelial cells (EA. hy926 cells) injury induced by particulates with no more than 2.5 μm air aerodynamic diameter in atmospheric (PM2.5),and its p38 mitogen-activated protein kinase(MAPK)signal pathway mechanism. METHODS:PM2.5 samples were collected and hatched EA.hy926 cells with concentrations of 0(blank control),20,200 and 400 mg/L for 24 h. The survival rate and apoptosis rate of cells,contents of IL-6,TNF-α and MDA,activities of SOD and LDH,protein levels of p-p38 MAPK,Bcl-2 and Bcl-2 associated X protein (Bax) were detected. The above indexes of EA.hy926 cells in blank control group,PM2.5 group (200 mg/L PM2.5), p38 MAPK pathway-specific blocker SB203580 group (20 μmol/L SB203580+200 mg/L PM2.5),berberine low-,medium- and high-concentrations groups(5,10,20 μmol/L berberine+200 mg/L PM2.5)were also determined. RESULTS:Compared with blank control,survival rate of cells,SOD activity and Bcl protein decreased after 200,400 mg/L PM2.5 hatched;apoptosis rate of cells, contents of IL-6,TNF-α and MDA,LDH activity,protein levels of p-p38 MAPK and Bax increased (P<0.05),in concentra-tion-dependent manner. Compared with PM2.5 group,survival rate of cells,SOD activity and Bcl-2 protein increased in berberine medium-,high-concentrations groups and SB203580 group;apoptosis rate of cells,contents of IL-6,TNF-α and MDA,LDH ac-tivity,protein levels of p-p38 MAPK and Bax decreased (P<0.05). CONCLUSIONS:Berberine attenuates PM2.5-induced EA. hy926 cells injury via the inhibition of p38 MAPK pathway.

Objective To investigate the comparison of the clinical treatment efficacy of propafenone and amiodarone in patients with paroxysmal supraventricular tachycardia ( PSVT ) .Methods Retrospective a total of 89 patients with PSVT from June 2013 to June 2015 in our hospital were selected.According to the different administration methods, the patients were divided into the propafenone group of 46 cases and the amiodarone group of 43 cases.The changes of left ventricular end-systolic volume, left ventricular end-diastolic volume, ejection fraction, cardioversion success rate, mean cardioversion time, heart rate and side effects were compared.Results After treatment, the left ventricular end-systolic volume (52.31 ±8.34) mL/m2 , (54.28 ±7.23) mL/m2, left ventricular end-diastolic volume (47.31 ±8.56) mL/m2, (45.28 ±7.16) mL/m2 and ejection fraction (61.57 ± 0.76)%, (61.39 ±0.69)% were without significant differences.After treatment, the heart rates of the two groups were significantly decreased, the propafenone group ( 85.31 ±12.84 ) times/min, amiodarone group ( 87.26 ±11.95 ) times/min, and there were not significant differences.The recovery time of propafenone group was ( 19.34 ±4.76 ) , higher than the amiodarone group ( 25.69 ±6.09 ) ( P <0.05 ).But the success rate of cardioversion (52.17%) was lower than the amiodarone group (88.37%) (P<0.05).The side effect rates in the propafenone group (21.74%) was significantly higher than that in the amiodarone group ( 4.65%) ( P <0.05 ) .Conclusion Propafenone and amiodarone cardioversion PSVT have achieved good results, but propafenone rapid onset, the average cardioversion time is short, which is only suitable for acute tachycardia and without serious organic heart disease patients.Amiodarone onset is slower,but the cardioversion success rate is higher.

Objective To investigate the efficacy of tolterodine plus tamsulosin in rats with benign prostatic hyperplasia(BPH)induced low urinary tract syndrome(LUTS).Methods Male rats were castrated,then testosterone propionate were given by subcutaneous injection to induce BPH/LUTS model.Model rats were intragastrically administrated with 0.4mg/kg of tolterodine,0.04mg/kg of tamsulosin or(0.4+0.04)mg/kg of tolterodine plus tamsulosin for 14 days and urodynamics,residual urine volume and volume of bladder were mearsured.Results Tolterodine plus tamsulosin could simultaneously shorten the micturition duration,prolong the interval duration between micturition,decrease the threshold intravesical pressure at micturition and the max intravesical Pressure at micturition significantly.The combined-therapy method could also decrease the residual urine volume to a certein extent and had no obvious influence on enlarging the volume of bladder(P＜0.05 or P＜0.01).Conclusion Combination treatment with tolterodine and tamsulosin may efficiently ameliorate bladder outlet obstrution and overactive bladder simultaneously,with no extra danger of uroschesis.