Objective To investigate the clinical manifestation and therapeutic result of 60 cases of intrahepatic cholestasis of pregnancy.Methods The clinical manifestation and treatment on 60 cases with intrahepatic cholestasis of pregnancy(ICP)Retrospectively analyzed.Results 60 patients with ICP were showed cutaneous pruritus.Compared with the pre-ICP group,AST,ALT,TBIL、DBIL and TBA of patients in the after-ICP group were obviously increased,and perinatal mortality of patients in the after-ICP group were obviously increased.Conclusion It was a key point for reducing the death rate by means of earlier diagnosis,earlier treatment,earher monitor and to terminate pregnancy with an adequate laboring way.

Objective To compare therapeutic effect on two surgical methods of uterus myoma between lifting laparoscopic surgery and open surgery, and evaluate the advantage,disadvantage and surgical indication for lifting laparoscopic surgery. Methods The clinical data of uterus myoma patients separately from lifting laparoscopic surgery(79 cases, group A) and open surgery (87 cases, group B) were retrospectively analyzed,the data such as total bleeding volume,operation time, anus exhausting time,postoperative hospitalization time, period of temperature returning to normal, and abdominal scar, catheter retention time and postoperative using analgesics were analyzed between two groups. Results The total bleeding volume,anus exhausting time,catheter retention time, period of temperature returning to normal, postoperative hospitalization time in group A [(58.4 ± 31.6) ml,(19.5 ± 2.5) h,(11.1 ± 2.5) h,(1.3 ± 0.8) d, (5.1 ± 1.9)d] were lower than those in group B [(99.1 ±45.6) ml, (35.8 ±4.0) h, (21.5 ±2.4) h, (2.6 ± 1.3) d,(10.4 ± 2.4) d](P ＜ 0.01). The postoperative using analgesics rate in group A was lower than that in group B [34.2%(27/79) vs. 66.7%(58/87)] (P ＜ 0.01), and abdominal scar was not obvious. Conclusion Lifting laparoscopic surgery treatment of uterus myoma is superior to the open surgery treatment.

0.025 63 h-1).A linear relation between absorption and concentration was noted for simvastatin at a concentration of 1.0～20.0 ?g?mL-1;and within a pH value of 5.0～7.4,the absorption of simvastatin was not affected.CONCLUSION:Simvastatin showed absorption behavior in the whole section of intestine.The absorption of the drug conforms to the passive transport mechanism and first-order kinetics.The results indicate that simvastatin can be formulated and prepared into sustained-release drug delivery system.

Because of the effects of misbalance of Qi/Blood and Yin/Yang and abuse of medicine,food and emotion,phlegm and static blood syndrome is easy to be made in the course of pathological changes of migraine.Phlegm and static blood syndrome is the important factor in the occurrence and development of migraine. In the whole period of migraine it is showing not only stabbing pain immovable for a long time,but also showing languor and scarce of appetite;The method of dissipating blood stasis and phlegm should be used effectively.

OBJECTIVE To investigate the mechanism and treatment of diabetes among elderly patients from the view of traditional Chinese medicine(TCM).METHODS On the basis of clinical experience and the achievement of the modern medicine,we expounded the mechanism of diabetes among elderly patients and treat from eight parts.RESULTS Using eight different TCM methods to treat diabetes elderly patients,we found their good curative effect with less adverse reaction,good prognosis,and very suit for old man.CONCLUSIONS TCM has large potentiality and advantage among diabetes elderly patients complicated with respiratory tract infection.

Objective To know the renal damage in cadmium (Cd) exposed residents in Cd polluted areas where polluted by Cd for 20 years. Methods The levels of urinary Cd (UCd), N-acetyl-beta-D-glucosaminidase (UNAG), ?2-microglobulin (UBMG), retinal binding protein (URBP), and albumin (UALB) were measured in 39, 65, 57, and 42 residents lived in the severe-, moderate-, light-, and non-polluted area respectively for more than 20 years. The test indexes were adjusted with urinary creatinine, and geometric means were statistically used. Results The levels of UCd in all subjects were obviously decreased compared with those in 1992, but the levels of UCd, UNAG, UBMG, and URBP and the abnormal rate of UNAG in residents in Cd-polluted areas were significantly higher than those in residents in non-polluted area (P

OBJECTIVE:To study pharmacokinetics and bioequivalence of Simvastatin sustained release tablets in healthy beagle dogs.METHODS:6 beagle dogs were randomized into two groups.They received oral single dose of Simvastatin sustained release tablets 20 mg(test tablet) or Simvastatin tablets for sale(reference tablet).Plasma samples were collected at different time points.Plasma concentration of simvastatin acid was determined by LC-MS/MS and pharmacokinetic parameters were calculated.RESULTS:The pharmacokinetic parameters of reference tablet vs.test tablet were as follows:Cmax:(23.461?6.043) ng?mL-1 vs.(13.942?3.236) ng?mL-1;tmax:(2.158?0.396) h vs.(4.116?1.145 3) h;t1/2:(4.564?0.645) h vs.(8.143?0.679) h;AUC0～24 h:(118.647?31.989) ng?h?mL-1 vs.(129.977?29.853) ng?h?mL-1.CONCLUSIONS:The absorption rate of test tablet is different from that of reference tablet.The two kinds of tablets are not bioequivalent.

AIM:To investigate the changes in parameters related to myocardial fibrosis of H2 relaxin (H2RLX) and procollagen type I C-terminal peptide (PICP),transforming growth factor-?1 (TGF-?1) level in the plasma after acute anterior myocardial infarction. METHODS:Forty-one patients with anterior myocardial infarction (MI) and 10 controls with normal coronary vessels were selected. Plasma H2RLX,PICP,and TGF-?1 levels were determined by enzyme linked immunosorbent assay (ELISA) in 10 control subjects and 41 post-MI patients at day 3 and 7 post-MI. RESULTS:(1) H2RLX level at day 3 post-infarct had no difference between the patients and the controls. No marked change in H2RLX at day 3 versus day 7 post-infarct was observed. (2)The level of PICP at day 3 post-infarct was markedly increased followed by a fall at day 7 post-infarct,but was still higher than that in control. (3) TGF-?1 level,which was similar at day 3 and 7 post-infarct,was higher than that in control. (4) At day 7 post-infarct,the level of H2RLX was positively correlated with the fasting blood glucose in MI group. CONCLUSION:In early period of acute MI,profibrotic factors such as TGF-?1 expression and collagen expression increase. However,antifibrotic relaxin has no change.

OBJECTIVE: To evaluate the effect of hypoxia improvement in Hep-2 cell on cisplatin-induced apoptosis. METHOD: Human laryngeal squamous cell carcinoma Hep-2 cells and HIF-1α-RNAi-Hep-2 cells were cultured in normoxic, hypoxic and reoxygenation condition. The inhibition of cisplatin on cell proliferation was evaluated by MTT assay. The influence of cisplatin on cell cycle and apoptosis were detected by flow cytometry. RESULT: The inhibition of cisplatin on cell proliferation was reduced by hypoxia. After HIF-1α gene was silenced, the inhibition of cisplatin on Hep-2 cell proliferation was increased apparently, but was still interfered partly by hypoxia. Hypoxia could induce cell apoptosis decreased and enhance chemotherapeutic resistance. After reoxygenation, cell apoptosis induced by cisplatin was increased significantly (P < 0.05). HIF-1α-RNAi-Hep-2 cells under hypoxia also showed certain resistance to apoptosis but the sensitivity to cisplatin was higher than that of Hep-2 cells. When cells were returned from hypoxic condition to normoxic condition for some time, the apoptosis induced by cisplatin was increased significantly (P < 0.05). CONCLUSION The improvement of hypoxic microenvironment with HIF-1α gene knockout could increase the sensitivity of Hep-2 cells to chemotherapy.
Apoptosis ; Carcinoma, Squamous Cell ; pathology ; Cell Cycle ; Cell Hypoxia ; Cell Line, Tumor ; drug effects ; Cell Proliferation ; drug effects ; Cisplatin ; pharmacology ; Flow Cytometry ; Gene Knockout Techniques ; Head and Neck Neoplasms ; pathology ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit ; metabolism ; Laryngeal Neoplasms ; pathology ; RNA Interference ; Squamous Cell Carcinoma of Head and Neck

The objective of this study is to compare the normalized prediction distribution errors (NPDE) and the visual predictive check (VPC) on model evaluation under different study designs. In this study, simulation method was utilized to investigate the capability of NPDE and VPC to evaluate the models. Data from the false models were generated by biased parameter typical value or inaccurate parameter inter-individual variability after single or multiple doses with the same sampling time or multiple doses with varied sampling time, respectively. The results showed that there was no clear statistic test for VPC and it was difficult to make sense of VPC under the multiple doses with varied sampling time. However, there were corresponding statistic tests for NPDE and the factor of study design did not affect NPDE significantly. It suggested that the clinical data and model which VPC was not fit for could be evaluated by NPDE.