AIM： To investigate the effects of 6， 7－ dimethoxycoumarins on the proliferation and the cell cycle of human lung carcinoma cell（ PA） in vitro METHODS： The effects of 6， 7－ dimethoxycoumarins on the cytomorphology， growth and cell cycle of PA cells were evaluated by MTT assay and flow cytometry in vitro RESULTS： 6， 7－ dimethoxycoumarins could inhibit the proliferation of PA cells in a dose－ dependent manner The inhibition action of 6， 7－ dimethoxycoumarins plateaued at 160μ g/ml(52 4％ inhibition) The agent in dose of 80μ g/ml led to a decline in the proportion of cells to those in G2/M phase， made the cells remain in G0/G1 phase and reduced the proliferation index(PI) of PA cells CONCLUSION： In vitro， 6， 7－ dimethoxycoumarins can inhibit the proliferation of PA cells， which leads to arrest of the cells in G0/G1 phase by way of inhibiting DNA synthesis

AIM:To investigate the effects of 6,7-dimethoxycoumarins on the proliferation and the cell cycle of human lung carcinoma cell(PA)in vitro METHODS:The effects of 6,7-dimethoxycoumarins on the cytomorphology,growth and cell cycle of PA cells were evaluated by MTT assay and flow cytometry in vitro RESULTS:6,7-dimethoxycoumarins could inhibit the proliferation of PA cells in a dose-dependent manner The inhibition action of 6,7-dimethoxycoumarins plateaued at 160?g/ml(52 4% inhibition) The agent in dose of 80?g/ml led to a decline in the proportion of cells to those in G2/M phase,made the cells remain in G0/G1 phase and reduced the proliferation index(PI) of PA cells CONCLUSION:In vitro,6,7-dimethoxycoumarins can inhibit the proliferation of PA cells,which leads to arrest of the cells in G0/G1 phase by way of inhibiting DNA synthesis

OBJECTIVE: To comprehend the epidemiological status and risk factors in hypertensive disorder complicating pregnancy (HDCP) in a district in Changsha, and to provide epidemiological evidence for early intervention of HDCP. METHODS: A total of 2 148 pregnant women in a district in Changsha from 2008 to 2009 were retrospectively analyzed and the related factors were evaluated by multivariate unconditional logistic analysis. RESULTS: The incidence of HDCP was 5.21% from 2008 to 2009. Multivariate unconditional logistic regression analysis showed that age (>35 years old)(OR=6.440,95%CI:2.987-13.884), body mass index (BMI) before pregnancy (OR=2.180,95%CI:1.619-2.936), and history of diabetes or kidney disease (OR=14.221,95%CI: 3.990-50.684) were risk factors for HDCP. CONCLUSION Age, BMI before pregnancy, and history of diabetes or kidney disease are risk factors for HDCP, while gravidity, parity, number of abortion and multiple gestation pregnancy have no obvious correlation with HDCP.
Adult ; Age Factors ; Body Mass Index ; China ; epidemiology ; Diabetes Mellitus ; Female ; Humans ; Hypertension, Pregnancy-Induced ; epidemiology ; etiology ; Incidence ; Logistic Models ; Multivariate Analysis ; Pregnancy ; Retrospective Studies ; Risk Factors

OBJECTIVE: To investigate the impact of renal denervation on the blood pressure, plasma renalase content and expression of renalase and tyrosine hydroxylase (TH) in the idney of spontaneous hypertensive (SH) rats and to explore the role of renal denervation in lowering the blood pressure. METHODS: SH rats were randomly assigned into a baseline group, a surgery (renal denervation) group, a sham group and a control group (n=48). WKY rats matched in age (n=12) served as a baseline control group. All rats were housed until 12 weeks old. Then, the rats in the baseline group and the WKY group were sacrificed whose blood and kidney were collected for examination. In the renal denervation group, the sham group and the control group, the blood pressure was monitored continuously. One week and 6 weeks after the renal denervation, 6 rats in each group were sacrificed whose blood and kidney were collected. ELISA was employed to measure the plasma renalase and Western blot assay done to detect the expression of TH and renalase in the kidney. RESULTS: Compared with WKY rats, blood pressure significantly increased and TH protein expression markedly elevated (P<0.05) in SH rats in the baseline group, but plasma renalase content and protein expression of renalase in the kidney dramatically reduced (P<0.05). One week after the surgery, the mean arterial pressure and TH protein expression in the surgery group were lowered compared with the baseline group and dramatically reduced compared with the sham group and the control group (P<0.05). In the surgery group, the renalase level was markedly increased compared with the baseline group, the sham group, and the control group (P<0.05). Six weeks after the renal denervation, the mean arterial pressure and TH level in the surgery group were significantly increased but the renalase content and expression markedly reduced compared with those 1 week, but there were no marked differences among the surgery group, the sham group, and the control group (P>0.05). No pronounced differences in the above variables were found between the sham group and the control group at any time point (P>0.05). CONCLUSION Renal denervation can lower the blood pressure, which may attribute to the suppression of sympathetic nerves, increase in plasma renalase content and renalase expression in the kidney.
Animals ; Blood Pressure ; physiology ; Hypertension ; surgery ; Kidney ; enzymology ; innervation ; Male ; Monoamine Oxidase ; blood ; metabolism ; Rats ; Rats, Inbred SHR ; Sympathectomy ; methods ; Sympathetic Nervous System ; physiopathology ; Tyrosine 3-Monooxygenase ; metabolism

BACKGROUND AND OBJECTIVEThe new edition of the TNM staging for lung and pleural tumours has been finished, which put weight on the extent of primary tumor as one of the important prognosises. But little study has performed on the primary tumor extent < or = 2 cm. The aim of this study is to explore the prognosis of patients with tumor extent < or = 2 cm in stage I of non-small cell lung cancer, which helps us to choose the best treatment for these patients.

METHODSRetrospective study on the clinical response and survival time of whom underwent complete surgical resection and diagnosed as T1a of stage I NSCLC from 1998 to 2004 was analyzed. Data was analyzed by SPSS 17.0 software.

RESULTSOverall survival rate was 80.8%. By the study, age (P = 0.241), gender (P = 0.175), history of smoking (P = 0.845), pathologic type ( P =0.265), and systematic mediastinal lymphadenectomy (SML )(P = 0.918) or not, postoperative adjuvant chemotherapy or not ( P = 0.616) and visceral pleural invasion (P = 0.827) were not the prognosises of these patients. Only the tumor differentiation such as poorly differentiated was the important prognosis ( P = 0.01).

CONCLUSIONIn the tumor extent < or = 2 cm of stage I non-small cell lung cancer, the visceral pleural invasion maybe not influence the patients survival. The tumor differentiation is one of the important prognostic factors.

Adult ; Aged ; Carcinoma, Non-Small-Cell Lung ; mortality ; pathology ; Female ; Humans ; Lung Neoplasms ; mortality ; pathology ; Male ; Middle Aged ; Prognosis ; Retrospective Studies

OBJECTIVETo examine the influence of China's economic reforms on population health and regional mortality rates.

METHODSLongitudinal study measuring the mortality trends and their regional variations. Using data from the three most recent national censuses, we used the model life table to adjust the mortality levels within the population for each census, and to calculate life expectancy. We then examined the variation in patterns of mortality and population health by economic status, region and gender from 1980-2000.

RESULTSLife expectancy varied with economic status, province, and gender. Results showed that, although life expectancy in China had increased overall since the early 1980s, regional differences became more pronounced. Life expectancy for populations who live in the eastern coastal provinces are greater than those in the western regions.

CONCLUSIONDifferences in life expectancy are primarily related to differences in regional economic development, which in turn exacerbate regional health inequalities. Therefore, it is necessary to improve economic development in less developed regions and to improve health policies and the public health system that address the needs of everyone.

China ; Developing Countries ; Economics ; Female ; Healthcare Disparities ; economics ; Humans ; Infant ; Infant Mortality ; Life Expectancy ; Male ; Mortality ; trends ; Sex Characteristics