Objective To study the relationship between the abnormality of mitochondrinl DNA (mtDNA) copy number and the clinical parameters and microsatellite instability (MSI) in colorectal cancer. Methods Total DNA was extracted from cancer and pericancer tissue from 50 colorectal cancer (CRC) biopsy samples. Non-ceding region sequencing was done and the copy number of mtDNA was quantitated with real-time PCR in mitochondrial NDI gene. The relationship between clinical indicators, mtMSI and mitochondrial copy number was detected. Results The mean copy number of mtDNA 312±185 in the tumor tissue was significantly lower than that 525±125 of the corresponding non-tumor tissue of these patients (P<0.001). No significant correlation was found between mtDNA copy number and other variables including age, gender, pathological type and clinical stage (P > 0. 05). However, there was a significant correlation between copy number and mtMSI (P<0.001). Conclusion There is a significant reduction of mtDNA in CRC patients, which may be caused by mtMSL

OBJECTIVE:To investigate the correlation of blood concentrations of tamoxifen (TAM) and its metabolites with endometrial hyperplasia in estrogen receptor(ER)-positive breast cancer patients. METHODS:A total of 69 patients with ER-posi-tive breast cancer selected from our hospital during Mar. 2015-Apr. 2016 received TAM (twice a day,one tablet each time) for more than 6 months. According to endometrial thickness,they were divided into abnormal hyperplasia group(40 cases)and normal group(29 cases). The steady state concentrations of TAM and its metabolites [4-OH-tamoxifen(OHT),N-demethylation tamoxifen (DMT),Endoxifen] were determined by HPLC-FLU. The correlation of blood concentration and other factors with endometrial thickness were investigated by Pearson test and multiple regression analysis. RESULTS:The medication time and Endoxifen steady state concentration in abnormal hyperplasia group were both significantly longer or higher than normal group,with statistical signifi-cance (P<0.05). There was no statistical significance in age,BMI,complication and steady state concentrations of TAM,OHT and DMT between 2 groups(P>0.05). The endometrial thickness was positively correlated with Endoxifen steady state concentra-tion and medication time(r=0.447,0.460,P<0.05). Using endometrial thickness(y)as dependent variable,medication time(x1) and Endoxifen steady state concentration(x2)as independent variable,multiple regression analysis was conducted. Multiple regres-sion equation was calculated as follows:y=2.436+0.123x1+0.082x2(F=12.610,r=0.526,P<0.05). CONCLUSIONS:Medication time and Endoxifen steady state concentration may be related to endometrial hyperplasia,which can provide reference for predicting TAM induced endometrial abnormal hyperplasia in ER-positive breast cancer patients.

Objective:To study the correlation between plasma concentration and clinical efficacy in newly diagnosed type 2 diabe-tes treated with nateglinide. Methods:On the basis of diet control and exercise, 73 cases of newly diagnosed type 2 diabetes received nateglinde therapy for 2 months. Adverse events were routinely monitored during the therapy. Fasting blood glucose(FBG), 2h post-prandial blood glucose(2h-PG), fasting C-peptide(F-CP), 2h C-peptide(P-CP) and glycosylated hemoglobin (HbA1c) were ob-served before and after the treatment. LC-MS was used to determine the plasma concentration of nateglinide on the last day of treat-ment. Results:FBG, 2h-PG, HbAlc and P-CP after the treatment had significant changes when compared with those before the treat-ment (P<0. 05). There was no significant difference in F-CP in spite of minor increase (P>0. 05). The difference in HbA1c and P-CP before and after the treatment both showed a significantly positive correlation with plasma concentration of nateglinide (P<0. 05). Conclusion:Nateglinide displays good clinical efficacy and safety in newly diagnosed type 2 diabetes, and its plasma concentration can be used to evaluate the pancreatic islets function and glucose-lowing effects.

Objective To investigate the clinical value of clinical application in diagnosis of prostate cancer (PCa) by prostate biopsy guided by rectal ultrasound contrast guided biopsy.Methods One hundred and ninety-eight patients with prostate in Punan Hospital of Pudong New District of Shanghai were investigated.According to different detection methods, the research objects were divided into two groups, the patients were performed with the ultrasound contrast guided biopsy as the imaging group (n =96), the patients with Doppler ultrasound guided biopsy as the ultrasonic group(n =102).The puncture Results were compared with pathological diagnosis.The positive rate of PCa and number of puncture needle were compared with the two puncture methods.The value of the application of the prostate biopsy guided by rectal ultrasound in diagnosis of prostate cancer was evaluated.Result One hundred and ninety-eight patients were all received pathological diagnosis,78 cases benign lesions, 120 cases were diagnosed as PCa.Thirty-six cases of benign lesions were confirmed by pathological biopsy, 60 case PCa.There were 42 cases of benign lesions in ultrasonic group, 60 case PCa.The positive rate of PCa in the imaging groupwas 62.5％ (60/96), the ultrasonic group was 58.82％ (60/102).There was no difference in Pca positive rate between the ultrasound group and the contrast group(x2 =0.104, P=0.747).The positive number of Pca in the imaging group was 28.50％ (17/60), the difference was statistically significant higher than that of the common group(18.80％ (11/60), P =0.001).The average of the patients in the imaging group was 8.19 needle,less than the ultrasonic group per capita 11.31 needle less.When f/tPSA less than or equal to 0.15, Pca positive rate of the contrast group was 46.55％ (27/55), higher than the ultrasonic group(9.30％ (4/43)), the difference was statistically significant (P =0.001);when f/tPSA more than 0.15, the positive rate of Pca in the contrast group was 92.11％ (35/38), less than the ultrasound group (94.92 (56/59)), the difference was not statistically significant (P =0.89).Anal pain, hematuria and hematochezia in contrast group (7.29％ (7/96), 2.08％ (2/96), 10.42％ (10/96)) were significantly less than the ultrasound group(22.55％ (23/102), 8.82％ (9/102), 23.53％ (24/102)), the difference was statistically significant (P =0.003,0.039,0.014).Conclusion Under the guidance of ultrasound contrast, rectal biopsy has important diagnostic value for prostate cancer.Under the premise of reducing the number of puncture needle,can improvethe positive rate of Pca, reduce the pain of patients and the occurrence of complications after puncture.When f/tPSAless than or equal to 0.15,puncture positive rate in contrast group is higher than the ultrasonic group, puncture effect is better.

AIM: To construct the replicative defecient adenovirus Ad-Runx3 expressing Runx3, and to express it in U251 malignant glioblastoma cells. METHODS: The runx3 gene with a flag tag was amplified by PCR using pCMV5-AML2 as a template, and was confirmed by DNA sequencing. The adenovirus shuttle vector pShuttle-CMVRunx3 was constructed by introducing runx3 DNA fragment into the sites of Kpn Ⅰ and Xho Ⅰ of pShuttle-CMV vector. This recombinant plasmid was linearized by Pmel and electronically transfected into BJ5183 cells to get the recombinant adenovirus vector Ad-Runx3. The recombinant adenovirus expressing Runx3 was infected into U251 malignant glioblastoma cells. The expression of exogenous Runx3 was observed by immonoblotting and its localization was detected by immunostaining using anti-Flag tag antibody. RESULTS: The recombinant adenovirus expressing Runx3 with a Flag tag was constructed and infected into U251 glioblastoma cells. The exogenous Runx3 protein was detected only in the nuclei. CONCLUSION: The recombinant adenovirus expressing Runx3 with a Flag tag is constructed successfully, and the Runx3 protein expressed in the nuclei of infected cells. The study laid a foundation for further research of the function of Runx3 in gliocarcinogenesis.

Obj ective To invesyigaye yhe clinical effecy of differeny approaches in yhe yreay-meny of dysphagia in payienys wiyh cerebral syroke.Methods 1 12 syroke payieny were randomly di-vided inyo yhree groups.A group was yreayed wiyh swallowing yraining and acupuncyure records u-niyed wiyh elecyric syimulayion yherapy.B group was yreayed wiyh swallowing yraining uniyed wiyh elecyric syimulayion yherapy.C group was yreayed wiyh swallowing yraining merely.All groups were given drinking wayer yesy yo evaluaye swallowing funcyion on yhe hospiyalized day and yhe 2 1 sy day of yhe yreaymeny.Results The yoyal effecyive raye of A group was 93 .7%,B group was 78 .9%,C group was 70 .2%.The yoyal effecyive raye of A group is superior yo yhay of B and C groups wiyh sya-yisyically significany(P<0 .05 ).The difference was syayisyically significany comparing A group and B group(P<0.05).The difference was syayisyically significany in drinking wayer yesy score and swal-lowing funcyion score before and afyer yreaymeny of yhe yhree groups.The resulys revealed yhay yhe yhree meyhods could improve dysphagia of payienys,buy yhe overall effecy of A group was superior yo yhay of B and C group.Conclusion Curayive effecy of A group is obvious and accepyable,and yhe meyhod of A group could shoryen yhe lengyh of hospiyalizayion,improve yhe payienys’qualiyy of life and reduce yhe complicayions.

Obj ective To invesyigaye yhe clinical effecy of differeny approaches in yhe yreay-meny of dysphagia in payienys wiyh cerebral syroke.Methods 1 12 syroke payieny were randomly di-vided inyo yhree groups.A group was yreayed wiyh swallowing yraining and acupuncyure records u-niyed wiyh elecyric syimulayion yherapy.B group was yreayed wiyh swallowing yraining uniyed wiyh elecyric syimulayion yherapy.C group was yreayed wiyh swallowing yraining merely.All groups were given drinking wayer yesy yo evaluaye swallowing funcyion on yhe hospiyalized day and yhe 2 1 sy day of yhe yreaymeny.Results The yoyal effecyive raye of A group was 93 .7%,B group was 78 .9%,C group was 70 .2%.The yoyal effecyive raye of A group is superior yo yhay of B and C groups wiyh sya-yisyically significany(P<0 .05 ).The difference was syayisyically significany comparing A group and B group(P<0.05).The difference was syayisyically significany in drinking wayer yesy score and swal-lowing funcyion score before and afyer yreaymeny of yhe yhree groups.The resulys revealed yhay yhe yhree meyhods could improve dysphagia of payienys,buy yhe overall effecy of A group was superior yo yhay of B and C group.Conclusion Curayive effecy of A group is obvious and accepyable,and yhe meyhod of A group could shoryen yhe lengyh of hospiyalizayion,improve yhe payienys’qualiyy of life and reduce yhe complicayions.