Objective To investigate the application value of montelukast in patients with stable chronic obstructive pulmonary disease (COPD).Methods 64 patients with stable COPD in Jinqiu Hospital of Liaoning Province from December 2015 to December 2016 were randomly divided into observation group and control group according to the digital table,32 cases in each group.The two groups were given routine treatment,including cough,phlegm,oxygen therapy,smoking cessation,rehabilitation treatment,regular use of long-acting bronchodilators,on this basis,the observation group was treated with montelukast sodium.After 12 weeks of treatment,the patients were assessed for airway improvement and quality of life before and after treatment,and recorded the two groups of acute exacerbation and adverse drug reactions.Results The two groups had improved lung function and quality of life after treatment,but after treatment,the FEV1,FVC and FEV1 actual/FEV1 prediction of the observation group were significantly higher than those of the control group,the differences were statistically significant (t =2.49,1.77,1.85 all P ＜ 0.05).After treatment,the 6 minutes walking distance of the observation group was longer than that of the control group,Borg score of the observation group was lower than that of the control group,the differences were statistically significant (t =1.83,6.66,all P ＜0.05).After treatment,the SGRQ score in the observation group was lower than that of the control group,the difference was statistically significant[(51.15 ± 3.14)points vs.(54.83 ± 4.03)points,t =3.94,P ＜ 0.01].Conclusion Montelukast can effectively improve the stability of COPD pulmonary function and quality of life,and with high safety.

Aim To investigate radioprotective effects of platelet factor 4 (PF4) on hemopoiesis of mice after γ -ray irradiation and their mechanisms. Methods The mice were injected with PF4 twice at 6h intervals; and 20 h after the second injection they were given one irradiation of 7.5Gy 60Co γ -ray. 8d later the colony forming unit of granulocyte-macrophage (CFU-GM), colony forming of unit spleen (FU-S), number and DNA contents in bone marrow cells (BMCs) were examined by flow cytometry. Results 8d after irradiation, a significant difference was shown between DNA contents in BMCs and cell cycles of the PF4-injected mice and those of the irradiated mice (P∨ 0.01), whereas little difference was found between those of the irradiated mice and the controls (P∧ 0.05). Conclusion PF4 can obviously protect hemopoietic stem cells (HSCs) of mice from radiation injury and promote proliferation of HSCs after radiation.

Objective To evaluate the role of P2X3 receptors in dorsal root ganglion in development of incisional pain in rats.Methods Twenty-four healthy male SD rats weighing 200-220 g were randomly divided into 3 groups(n = 8 each): control group(group C),incisional pain(group IP)and P2X3 receptor antagonist + IP group(group A).In group IP and A,a 1 cm longitudinal incision was made in the plantar surface of left hindpaw according to the method described by Brennan et al.in isoflurane-anesthetized rats.P2X3 receptor antagonist TNP-ATP 200 nmol was injected into the plantar surface of left hindpaw 30 min after plantar incision was made in group A,while equal volume of normal saline was given instead of TNP-ATP in group C and IP.The behavior of the hindpaw of the rats were assessed using cumulative pain score within 1 h after injection.The animals were sacri ficed 2 h after injection and the dorsal root ganglion was removed for determination of P2X3 receptor expression and intracellular Ca2+ concentrations.ResultsThe cumulative pain scores,P2X3 receptor expression and Ca2 + concentrations were significantly higher in group IP and A than in group C(P ＜ 0.05).The cumulative pain scores,P2X3 receptor expression and Ca2+ concentrations were significantly lower in group A than in group IP(P ＜0.05).Conclusion P2X3 receptors in dorsal root ganglion is involved in the development of incisional pain through increasing intracellular Ca2+ concentrations in rats.

AIM: To construct the replicative defecient adenovirus Ad-Runx3 expressing Runx3, and to express it in U251 malignant glioblastoma cells. METHODS: The runx3 gene with a flag tag was amplified by PCR using pCMV5-AML2 as a template, and was confirmed by DNA sequencing. The adenovirus shuttle vector pShuttle-CMVRunx3 was constructed by introducing runx3 DNA fragment into the sites of Kpn Ⅰ and Xho Ⅰ of pShuttle-CMV vector. This recombinant plasmid was linearized by Pmel and electronically transfected into BJ5183 cells to get the recombinant adenovirus vector Ad-Runx3. The recombinant adenovirus expressing Runx3 was infected into U251 malignant glioblastoma cells. The expression of exogenous Runx3 was observed by immonoblotting and its localization was detected by immunostaining using anti-Flag tag antibody. RESULTS: The recombinant adenovirus expressing Runx3 with a Flag tag was constructed and infected into U251 glioblastoma cells. The exogenous Runx3 protein was detected only in the nuclei. CONCLUSION: The recombinant adenovirus expressing Runx3 with a Flag tag is constructed successfully, and the Runx3 protein expressed in the nuclei of infected cells. The study laid a foundation for further research of the function of Runx3 in gliocarcinogenesis.

AIM:To investigate the expression of angiotensin-converting enzyme 2 (ACE2) in nephritic epithelium of primates. METHODS:The expression of ACE2 in Vero E6 cells was detected by the techniques of RT-PCR and immunocytochemistry (ICC) techniques. The distribution of ACE2 protein in kidney tissues of two Rhesus monkeys and two normal human cases was observed by immunohistochemistry (IHC) techniques. RESULTS:Vero E6 cells were found to express both ACE2 mRNA and protein. ACE2 protein was mainly located in epithelium of proximal tubules of Rhesus monkey and human kidney. CONCLUSION:The expression of ACE2 in epithelium of primate kidney may provide the condition for SARS-CoV entry,which may be one of the reasons for inducing renal damage in SARS patients.

Objective To summarize clinical observation and nursing of 36 patients with gestational diabetes complicated with hypertension during caesarean perioperation.Methods A to-tal of 36 patients with gestational diabetes complicated with hypertension used holistic nursing and additionally strengthened maternal and neonatal illness observation,blood glucose,blood pressure management,prevention of infection and venous thrombosis and health care and education measures based on the conventional nursing.Results All the 36 patients successfully completed the cesarean section operation without maternal and neonatal mortality,puerperae and children were smooth dis-charged without complications.Conclusion Comprehensive nursing measures,careful clinical ob-servation and safe,effective nursing measures ensure the safety of patients with gestational diabetes complicated with hypertension.

Objective To summarize clinical observation and nursing of 36 patients with gestational diabetes complicated with hypertension during caesarean perioperation.Methods A to-tal of 36 patients with gestational diabetes complicated with hypertension used holistic nursing and additionally strengthened maternal and neonatal illness observation,blood glucose,blood pressure management,prevention of infection and venous thrombosis and health care and education measures based on the conventional nursing.Results All the 36 patients successfully completed the cesarean section operation without maternal and neonatal mortality,puerperae and children were smooth dis-charged without complications.Conclusion Comprehensive nursing measures,careful clinical ob-servation and safe,effective nursing measures ensure the safety of patients with gestational diabetes complicated with hypertension.

Obj ective To invesyigaye yhe clinical effecy of differeny approaches in yhe yreay-meny of dysphagia in payienys wiyh cerebral syroke.Methods 1 12 syroke payieny were randomly di-vided inyo yhree groups.A group was yreayed wiyh swallowing yraining and acupuncyure records u-niyed wiyh elecyric syimulayion yherapy.B group was yreayed wiyh swallowing yraining uniyed wiyh elecyric syimulayion yherapy.C group was yreayed wiyh swallowing yraining merely.All groups were given drinking wayer yesy yo evaluaye swallowing funcyion on yhe hospiyalized day and yhe 2 1 sy day of yhe yreaymeny.Results The yoyal effecyive raye of A group was 93 .7%,B group was 78 .9%,C group was 70 .2%.The yoyal effecyive raye of A group is superior yo yhay of B and C groups wiyh sya-yisyically significany(P<0 .05 ).The difference was syayisyically significany comparing A group and B group(P<0.05).The difference was syayisyically significany in drinking wayer yesy score and swal-lowing funcyion score before and afyer yreaymeny of yhe yhree groups.The resulys revealed yhay yhe yhree meyhods could improve dysphagia of payienys,buy yhe overall effecy of A group was superior yo yhay of B and C group.Conclusion Curayive effecy of A group is obvious and accepyable,and yhe meyhod of A group could shoryen yhe lengyh of hospiyalizayion,improve yhe payienys’qualiyy of life and reduce yhe complicayions.

Obj ective To invesyigaye yhe clinical effecy of differeny approaches in yhe yreay-meny of dysphagia in payienys wiyh cerebral syroke.Methods 1 12 syroke payieny were randomly di-vided inyo yhree groups.A group was yreayed wiyh swallowing yraining and acupuncyure records u-niyed wiyh elecyric syimulayion yherapy.B group was yreayed wiyh swallowing yraining uniyed wiyh elecyric syimulayion yherapy.C group was yreayed wiyh swallowing yraining merely.All groups were given drinking wayer yesy yo evaluaye swallowing funcyion on yhe hospiyalized day and yhe 2 1 sy day of yhe yreaymeny.Results The yoyal effecyive raye of A group was 93 .7%,B group was 78 .9%,C group was 70 .2%.The yoyal effecyive raye of A group is superior yo yhay of B and C groups wiyh sya-yisyically significany(P<0 .05 ).The difference was syayisyically significany comparing A group and B group(P<0.05).The difference was syayisyically significany in drinking wayer yesy score and swal-lowing funcyion score before and afyer yreaymeny of yhe yhree groups.The resulys revealed yhay yhe yhree meyhods could improve dysphagia of payienys,buy yhe overall effecy of A group was superior yo yhay of B and C group.Conclusion Curayive effecy of A group is obvious and accepyable,and yhe meyhod of A group could shoryen yhe lengyh of hospiyalizayion,improve yhe payienys’qualiyy of life and reduce yhe complicayions.