AIM： To investigate the tumorigenicity of lung cancer by learning the accumulation of p53 and the exposure of cytokeratin 18 neo-epitope(CK-18neo) related to the clinicopathological parameters in non-small cell lung cancer(NSCLC). METHODS: To detect the monoclonal antibodies of p53 and M30 CytoDEATH(specific antibody for CK-18neo) in 62 cases of NSCLC (included 29 cases of squamous cell carcinoma and 33 cases of adenocarcinoma) and 10 cases of control group by adopting immunohistochemistry assay (LSAB). Moreover, the immunoreactivity of p53 was quantitatively evaluated with positive unit (PU). RESULTS: (1) p53 immunoreactivity was positive in 15 of 29 squamous cell carcinoma (51.72%), 15 of 33 adenocarcinoma (45.45%), 30 of 62 NSCLC (48.39%). In 10 control cases was negative. There were significant differences between these groups (P＜0.01). (2)In 62 cases of NSCLC, AI% of M30 is 1.10%, and in 29 cases of squamous cell carcinoma is 0.95%, and in 33 cases of adenocarcinoma is 1.24%. In 10 control cases, the AI% is 1.06%. There is not significant difference among these groups . (3) According to the results of Pearson's correlation analysis, we found positive linear correlation between the immunoreactivity of p53（-/+）, p53（5 degrees）and p53（PU）(P＜0.01). CONCLUSION: Our results suggested that the pathogenesis of NSCLC might be related to the mutation of gene p53 and cell excessive proliferation and insufficient apoptosis.

Objective To explore the impact of psychological intervention on response and defense style of AIDS patients.Methods 88 AIDS patients were selected and investigated using medical response (MCMQ) and defense style (DSQ) survey,the scores before and after psychological intervention were compared.Results Mental health problems included fear,afraid,pessimism and worry,the response style included face,avoid,yield.Scores showed significant difference before and after intervention among patients choosing immature-type,mature-type and cover-up defense styles.Conclusions Psychological intervention can promote rehabilitation and health of AIDS patients as well as improve their life quality and prevent disease relapse.

AIM: To investigate the tumorigenicity of lung cancer by learning the accumulation of p53 and the exposure of cytokeratin 18 neo-epitope(CK-18neo) related to the clinicopathological parameters in non-small cell lung cancer(NSCLC). METHODS: To detect the monoclonal antibodies of p53 and M 30 CytoDEATH(specific antibody for CK-18neo) in 62 cases of NSCLC (included 29 cases of squamous cell carcinoma and 33 cases of adenocarcinoma) and 10 cases of control group by adopting immunohistochemistry assay (LSAB). Moreover, the immunoreactivity of p53 was quantitatively evaluated with positive unit (PU). RESULTS: (1) p53 immunoreactivity was positive in 15 of 29 squamous cell carcinoma (51.72%), 15 of 33 adenocarcinoma (45.45%), 30 of 62 NSCLC (48.39%). In 10 control cases was negative. There were significant differences between these groups ( P

Objective To explore the influence of self care interaction mode on the health behavior of the patients with thoracolumbar fracture.Methods 127 cases of thoracolumbar fracture were randomly divided into the observation group (62 cases)and the control group (65 cases)according to random digital table method.The patients of the observation group were given self nursing interactive nursing.The patients of the control group were given rou-tine nursing.The comparative study of the following indicators of the two groups of patients:(1)activities of daily liv-ing (ADL);(2)bedridden patient self -care skills of and self -care knowledge assessment;(3)satisfaction,compli-cations,the average length of days and discharged patient telephone visit the initiative.Results The discharge self nursing ability raise,health behavior change,self -care knowledge and self -care skills of the observation group were significantly better than the control group,there were statistically significant differences(P <0.05);after discharge from the hospital,bed incidence of complications (6.5%)of the observation group was significantly lower than the control group (18.1%),the difference was statistically significant (χ2 =4.16,P <0.05)and satisfaction degree (91.7 ±6.5)was higher than the control group (87.3 ±7.5),with statistically significant difference (t =3.59,P <0.05),and the average hospitalization day (16.11 ±7.61 )d was lower than that of the control group (19.95 ± 11.55)d,there was statistically significant difference (t =2.20,P <0.05).Conclusion Self care interactive mode is conducive to mining the self -care ability of patients,improve the self -care ability of patients,and has good social benefits.

Objective To investigate the effect of nursing intervention on deep vein thrombosis after major bone trauma.Methods 59 cases of bone trauma surgery were selected as the research subjects,according to random number table method,they were randomly divided into the observation group and the control group.The patients of the control group received routine nursing care,and the patients of the observation group were given nursing intervention for postoperative deep vein thrombosis on the basis of the conventional nursing care.The pulmonary embolism,deep vein thrombosis and lower extremity swelling occurred rate before and after intervention of venous blood flow velocity, patients hospitalization time and quality of care scores were compared.Results The incidence rates of pulmonary embolism (0.0%),deep vein thrombosis (11.1%)and lower extremity swelling with postoperation (14.8%)of the observation group were significantly less than those in the control group(15.6%,53.1%,46.9%),there was signifi-cant differences (χ2 =4.61,11.54,6.89,all P <0.05).After intervention,the lower extremity venous blood flow velocity of two groups [(20.9 ±5.3)cm/s,(27.5 ±7.4)cm/s]was significantly higher than before intervention [(19.2 ±4.6)cm/s,(19.8 ±5.3)cm/s],there was statistically significant difference (t =1.37,4.40,all P <0.05).The stem prognosis of lower extremity venous blood flow velocity of the observation group [(27.5 ±7.4)cm/s]was significantly higher than (20.9 ±5.3)cm/s of the control group,there was significant difference (t =3.98, P <0.05).The hospitalization time in the observation group (45.2 ±5.4)d was less than (62.3 ±7.5)d in the con-trol group,and the nursing quality score (98.7 ±1.1)was higher than (91.8 ±1.7)in the control group,and the differences were statistically significant (t =9.88,18.12,all P <0.05).Conclusion Comprehensive nursing inter-vention can help improve venous blood flow velocity,reduce the incidence of deep vein thrombosis,shorten the hospital stay,and improve the quality of nursing.

Objective To observe the clinical efficacy of Wendan decoction in the treatment of phlegm-heat accumulation type acie vulgaris.Methods 62 patients with phlegm-heat accumulation type ache vulgaris were randomly divided into a treatment group and a control group randomly,with 31 patients in each group.The control group was given dietary advice and skin cleansing techniques,while the treatment group was given oral administration of Wendan decoction on the basis of the control group.Results ①skin damage comparison:after the treatment,skin damage symptoms such as tenderness (0.71 ±0.32),redness (0.47±0.63),cyst(0.59±0.53),and tubercle (0.63±0.54) in the treatment group were significantly improved than the control group [the values were (1.12±0.32),(1.44±0.21),(1.40±0.36),(1.18±0.43),respectively] (P＜0.05).②symptoms score comparison:The symptoms score in the treatment group after the treatment (42.95±1.53) was significantly improved than that before the treatment (52.89± 1.98),(P＜0.05),and also obviously better than the control group after the treatment.③clinical efficacy comparison:Total efficacy was 96.77％ in the treatment group and 54.83％ in the control group,showing marked difference(P＜0.05).Conclusion Wendan decoction is effective in treating phlegm-heat accumulation type acne vulgaris.

OBJECTIVETo compare therapeutic effects of electroacupuncture and TENS on senile radical sciatica.

METHODSOne hundred and thirty-nine cases were randomly divided into 2 groups, electroacupuncture group (n=70) and TENS group (n=69). The electroacupuncture group was treated with electroacupuncture at main points Qihaishu (BL 24), Dachangshu (BL 25), Guanyuanshu (BL 26), etc. and the TENS group was treated with skin electrode sticking at the pressure pain point of the nerve trunk which was connected with pulse current. Their therapeutic effects were observed and compared.

RESULTSAt the end of the first course, the cured rate was 41.4% in the electroacupuncture group and 29.0% in the TENS group with a significant difference between the two groups (P < 0.05), and at the end of second course, their cured rates were 80.0% and 44.9%, respectively, with a very significant difference between the two groups (P < 0.005).

CONCLUSIONThe therapeutic effect of electroacupuncture on senile radical sciatica is significantly better than TENS.

Objective To analyze the diagnosis and treatment for complications of renal caliceal diverticulum with calculi or infection. Methods A retrospective investigation was performed on 29 cases with renal caliceal diverticulum. The 29 cases included 11 males and 18 females aged 18 to 61 years. Among the study group, 3 cases were simple renal caliceal diverticulum, 12 cases were diagnosed as diverticular calculi and 14 cases presented recurrent urinary tract infections including 3 cases with urinary fistula after unroofing and decompression as renal simple cyst from another hospital. Ten cases underwent an open operation that unroofed and decompressed the cyst, and sutured the diverticular neck. Eight cases underwent laparoscopic operation similar to the open operation, including lithotomy in caliceal diverticulum in 2 cases. Eleven cases diagnosed with caliceal diverticular calculi were taken one-stage percutaneous nephrolithotomy including dilating the diverticular neck, remaining the nephrostomy catheter and Double-J ureteral stents, and 1 case was transferred to open operation.Results The open and laparoscopic operations were performed successfully. One case was cured by Double-J ureteral stenting after postoperative urinary leakage. One case was transferred to open operation for the failure of percutaneous puncturation. X-ray examination revealed that there were no remaining stones after the operation. All the patients were followed up for 6 to 24 months without calculi and infection recurrence. Conclusions Stones and infection are common that complications of renal caliceal diverticulum. Percutaneous nephrolithotomy, laparoscopy and other operations were effective and feasible treatment options for cases with complications of renal caliceal diverticulum. Exact diagnosis was very important for treatment of renal caliceal diverticulum before operation.

Objective:To investigate the role of serum RGS2 protein,Ang Ⅱ and AT1R in the Pathogenesis of Preeclampsia and it's impact on the severity of the disease.Methods:Totally 50 patients with PE collected from Shenzhen Maternity and Child Healthcare Hospital,Southern Medical University during October 2015-September 2016 were recruited as PE group(PE with FGR group 17 cases and PE without FGR group 33 cases),40 cases of healthy pregnant women were collected as the control group.ELISA was used to detect the serum RGS2 protein,Ang Ⅱ and AT1R levels.he differences between the groups were compared.Logistic regression was used to analyze the correlation between the three indexes and the severity of PE.Results:①In PE group plasma RGS2 protein,Ang Ⅱ,AT1R levels and systolic blood pressure,diastolic blood pressure,sampling gestational age,maternal gestational age,neonatal birth weight were higher than those in control group(P ＜ 0.05);There was no significant difference on RGS2 protein level,systolic blood pressure and diastolic blood pressure between PE with FGR group and PE without FGR group(P＞0.05),There was significant difference on the Ang Ⅱ,AT1R level and newborn weight(P ＜ 0.05).②Logistic regression analysis showed that RGS2 、Ang Ⅱ and AT1R were the independent risk factors of PE.(the crude odds ratio of RGS2,Ang Ⅱ and AT1R were ＞ 1,P ＜ 0.05.After adjust the sampling of gestational age the OR values were ＞ 1,P ＜0.05.After adjust the other two indicators the OR values were ＞ 1,P ＜0.05,except for Ang Ⅱ.).③RGS2 protein,Ang Ⅱ,AT1R levels were positively correlated with PE,systolic and diastolic blood pressure(P ＜ 0.05).Ang Ⅱ,AT1R levels were negatively correlated with neonatal birth weight (P ＜ 0.05).RGS2 was not related with neonatal birth weight (P ＞ 0.05).Conclusions:Plasma RGS2 protein,Ang Ⅱ and AT1R may be associated with the pathogenesis of PE and Ang Ⅱ and AT1R may be associated with the severity of the disease.

Epidemiology is one of main courses for undergraduate students majoring in preventive medicine.There are some limitations in the traditional epidemiology teaching,which is usually characterized in indoctrinated education:"lectured by the teachers and listened by the students." In Zhejiang University,staff of the epidemiology division tried to explore a new teaching mode as ‘student-centered,teacher-leading,question-based,and combining with literature discussion and course practice.'After practicing for two years,students were inspired in learning initiatives,with teaching effectiveness obviously improved.