Giant cell tumor (GCT) is rare and it mainly occurred in male patients. A man of 65 years-old with progressive dyspnea has a 4 cm x 4 cm x 3 cm tumor mass under the right vocal fold originated from right-thyroid cartilage and the parapharyngeal space. The right false vocal was involved in the computed tomography (CT) imaging and laryngoscope. Microscopically, the tumors had an expansile, infiltrative growth and consisted of numerous multinucleated osteoclast-like giant cells within a cellular stroma composed of plump and oval mononuclear cells. The patient underwent partial laryngectomy and has been followed up for 6 months without recurrence or metastasis.
Aged ; Giant Cell Tumors ; Humans ; Laryngeal Neoplasms ; Male

Objective To observe effect of abdomen compression on improvement of anal exsufflation for patients after abdomen operation. Methods Patients in the department of general surgery( 120 cases )after abdomen operations were randomly divided into the experimental group and the control group with 60 cases in each group.The control group adopted routine postoperative nursing,The experimental group received abdomen compression baaed upon routine nursing. Anal exsufflation time was compared between the two groups. Sults Anal exsufflation time of the experimental group undergoing different operations was earlier than that of the control group(P < 0.01 ). Conclusions Abdomen compression can bring forward anal exsufflation time,fa-cilitate the recovery of gastrointestinal function and normal diet, thus improve the rehabilitation of patients

OBJECTIVE: To discuss the role of carbon nanoparticles in the protection of parathyroid during thyroid carcinoma surgery. METHOD: Seventy-two patients with thyroid carcinoma who had initial surgery were randomly divided into two groups: carbon nanoparticles group and the control group. Emulsion of carbon nanoparticles was injected into the thyroid gland of carbon nanoparticles group patients. After thirty minutes,the excision of thyroid carcinoma and VI group neck dissection were performed in carbon nanoparticles group patients, the control group directly underwent operation. The black stained tissue in the dissection specimen of carbon nanoparticles group was separated. The number of total lymph node,metastasis lymph node and parathyroid gland in the tissure black stained or not in two groups were counted respectively. RESULTS: There were 312 lymph nodes in the black stained tissue of central compartment dissection specimen of carbon nanoparticles group. No parathyroid gland was found in the black stained tissue. Fifteen lymph nodes containing four parathyroid glands were found in the non black stained tissue in carbon nanoparticles group while there were 202 lymph nodes containing 13 parathyroid glands in the control group. There were statistical difference between the amount of lymph node in black stain tissue and the specimen of the control group. Parathyroid glands were not stained black,and no parathyroid gland was found in the black-stained tissue. CONCLUSION The carbon nanoparticles could be used to identify the lymph node and the parathyroid gland for protecting the parathyroid gland in thyroid surgery.
Carbon ; Carcinoma ; Coloring Agents ; Dissection ; Humans ; Lymph Nodes ; Lymphatic Metastasis ; Nanoparticles ; Neck Dissection ; Parathyroid Glands ; Thyroid Neoplasms ; pathology ; surgery

Objective To investigate the impact of lncRNA DSCAM-AS1 on the malignant biological behaviors of thyroid cancer(TC)cells by regulating the miR-150-5p/BRAF axis.Methods The expression of DSCAM-AS1 in TC cells was detected by qRT-PCR,and the best intervention cell line was screened.;the relationship between DSCAM-AS1,BRAF and miR-150-5p targeting regulation was verified by FISH,pull down and double Luciferase reporter gene experiment;The proliferation,migration and invasion of SW579 cells were detected;Western blot was applied to detect the expression of BRAF,E-Cadherin,and vimentin proteins;the tumor formation experiment in mice was applied to verify the effect of DSCAM-AS1 on TC tumor growth.Results DSCAM-AS1 was highly expressed in TC tissue and cells(P<0.05);There is a targeted regulation relationship between DSCAM-AS1,BRAF and miR-150-5p;inhibition of DSCAM-AS1 expression or overexpression of miR-150-5p obviously inhibited the proliferation,migration,invasion,and EMT of SW579 cells(P<0.05);Inhibition of miR-150-5p expression or overexpression of BRAF reversed the inhibitory effect of inhibition of DSCAM-AS1 expression or overexpression of miR-150-5p on the malignant behavior of SW579 cells(P<0.05);in vivo experiments showed that inhibiting the expression of DSCAM-AS1 obviously inhibited the growth of transplanted tumors in mice(P<0.05).Conclusion DSCAM-AS1 is up-regulated in TC cells,inhibiting the expression of LncRNA DSCAM-AS1 can inhibit the malignant progression of TC by regulating the miR-150-5p/BRAF signaling axis.

Objective : To investigate the role of member septin family (SEPT12)in human spermatogenesis and its influence on sperm motility and sperm ultrastructure. Methods : Whole exome sequencing (WES) was performed on peripheral blood DNA extracted from 375 patients with asthenoteratozoospermia , and a patient with idiopathic infertility carrying compound heterozygous mutation of SEPT12 was screened out. Sanger sequencing was performed to verify the mutation , and co⁃segregation analysis was performed in the family. The morphological abnormalities of sperm were analyzed by hematoxylin⁃eosin (HE) staining and scanning electron microscopy (SEM) , and the ultrastructural defects of sperm were analyzed by transmission electron microscopy (TEM) . Then the effects of the mutation on the level and position of the protein and the changes of the location and level of the defect structure markers were analyzed by Western blot and immune⁃fluorescence (IF) . Results : The compound heterozygous mutations c.C332A (p. T111K) and c. 406_416 del TGCTCGTATTG (p. q136 VFS ∗39) in the SEPT12 gene were screened and identified in a patient with asthenoteratozoospermia. The mutations were verified by Sanger sequencing , which was consistent with the co⁃segregation genetic pattern of the family. The mutations resulted in loss of protein expression , decreased sperm motility and sperm morphological deformities , mainly including short tail , curly tail and irregular sperm head. The ultrastructure of sperm showed that the annulus between the mid⁃piece and the principlepiece was missing , the acrosome membrane of sperm head fell off and the nucleus contained vacuoles. In the midpiece of sperm flagella , the arrangement of mitochondrial sheath was disordered , most of flagella axoneme central pair was absent , microtubules doublet was missing or disordered , and some radical spoke was absent. By Western blot and IF , the marker proteins of related structural components were detected , and the results showed that the level of SEPT4 protein decreased , SEPT6 protein unchanged , acrosomal related proteins ACTL7A and ACROSIN protein missing , and the expression levels of mitochondrial and axoneme related proteins TOMM20 , SPAG6 and RSPH3 protein significantly decreased. Conclusion The deletion of SEPT12 protein caused by SEPT12 gene mutation leads to the deletion of the annulus between the mid⁃piece and the principle⁃piece , and the abnormal assembly of sperm acrosome , mitochondrial sheath and flagella.