Objective To discuss the significance of the seminar in biochemistry teaching for Uygur clinical medical undergraduates. Methods 2011 grade totally 86 Uygur clinical medical under-graduates in Xinjiang Medical University were enrolled as the research object and questionnaire was used to make satisfaction survey. Data descript and analysis was performed according to the percentage of each option. Results 86.9%students thought that topic seminar was good;100.0%students thought that semi-nar can help apply and mastery the knowledge;100.0%students considered that seminar conducted dur-ing extracurricular time was necessary to the growth of knowledge;97.8%students considered that search-ing for references in the spare time was helpful;100.0%students were interested in the content of the seminar and thought it was novel and practical;100.0%students thought that seminar can help broaden thinking way;43.5%students supported that contents exceeding the syllabus can be used in examination while 39.1%students opposed; preparation time for examination lasted for 5 to 7 days. Conclusions Seminar applied in biochemistry teaching is very necessary,which can mobilize students' initiatives to learn actively and help consolidate and understand knowledge.

OBJECTIVE: To construct a NF-kappaB siRNA expression vector and to detect the specific silencing effect of the siRNA on the expression of NF-kappaB protein. METHODS: pcDNA3.1/CT-GFP-TOPO recombinant eukaryotic expression vector and pSilencer 1.0-U6-siRNA-NF-kappaB recombinant vector were constructed respectively. These 2 recombination plasmids were co-transfected into COS-7 cells, and the NF-kappaB silence induced by RNAi was detected by Western blot and the inverted fluorescence microscope. RESULTS: The levels of NF-kappaB protein in COS-7 cells could be silenced effectively and specifically by pSilencer 1.0-U6-siRNA- NF-kappa recombinant vector. The expression of NF-kappaB protein was reduced gradually with the increase of pSilencer 1.0-U6-siRNA- NF-kappaB recombinant vector,which could be detected by Western blot under the inverted fluorescence microscope. CONCLUSION NF-kappaB siRNA expression vector is constructed successfully.
Animals ; COS Cells ; Chlorocebus aethiops ; Genetic Vectors ; NF-kappa B ; genetics ; RNA Interference ; RNA, Small Interfering ; Transfection

OBJECTIVE: To investigate correlation between the change in metabolic components of serum and the abnormal balgam syndrome by using a rat model of abnormal balgam syndrome. METHODS: Male Wistar rats were randomly divided into a control group and a test group. According to Uyghur medicine theory, the test group of rats were given wet cold diet (seeds of spinach and parsley, 24 hours) in a cold (6 °C) and humid (85%-95%, 10 hours) environment for 40 days to establish the rat model of abnormal balgam syndrome. 1H MR based metabonomic analysis of serum was performed. Data was analyzed using Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA) software. RESULTS: Compared with the control group, the serum components including glutamate, phenylalanine, tyrosine, citric acid, β-hydrocxy butyrate, acetoacetate, pyruvic acid and creatine were decreased, while the glucose, lactic acid, low density lipoprotein and very low density lipoprotein were increased in the test group (P<0.05). CONCLUSION The low energy production and consumption in the rat model of abnormal balgam syndrome suggests that the dysfunctional metabolisms of three major nutrients might be the molecular basis for the abnormal balgam syndrome.
Animals ; Blood Glucose ; Carboxylic Acids ; blood ; Creatine ; blood ; Disease Models, Animal ; Lipoproteins, LDL ; blood ; Male ; Medicine, Chinese Traditional ; Metabolomics ; Rats ; Rats, Wistar ; Syndrome

Experimental teaching is an indispensable task in teaching work in colleges and universities.Basic medical experimental teaching is the foundation of clinical practice,techniques,and scientific research.Medical biochemistry experiment is the compulsory experimental teaching content for medical students.The existing basic medical experimental teaching is far from enough for medical students.Therefore,it is very important to tap the potential of students,cultivate their interest in learning,improve the ability to learn on their own,operate manually,be creative,and solve problems,which can start from basic medical experimental teaching and be achieved through the second class activities.