BACKGROUND: With the development of technology, poly-L-lactic acid/β-tricalcium phosphate composite materials show good characters in the tissue engineering, which can promote osteoblast proliferation, reduce rejection reactions, and improve bone healing in a dose-dependent manner. OBJECTIVE: To test the influence of poly-L-lactic acid with different molecule weights on the structure and function of poly-L-lactic acid/β-tricalcium phosphate composite scaffolds. METHODS: Poly-L-lactic acid with molecule weights of 200 000 and 380 000 were combined with β-tricalcium phosphate to produce composite scaffolds by using freeze-drying method. Porosity and pore size of the samples were measured. The fetal rabbit bone mesenchymal stem cells (BMSCs) were cultured and expanded in vitro. They were harvested and seeded into the prepared poly-L-lactic acid/β-tricalcium phosphate scaffolds. The 3-(4, 5-Dimethylthiazol-2yl)-2, 5 diphenyltetrazolium bromide (MTT) and alkaline phosphatase were examined for comparison between normal cultured BMSCs and those cultured on the different poly-L-lactic acid/β-tricalcium phosphate scaffolds. RESULTS AND CONCLUSION: Images of scanning electron microscope showed that the cells adhered to the scaffolds greatly. The value of MTT and alkaline phosphatase showed no significant difference (P > 0.05). The molecule weight of poly-L-lactic acid has no influence on the biological function of composite materials.

Objective: In recent years,botulinum toxin type A(BTX-A) has been investigated for the treatment of pain.This experiment was to investigate the antinociceptive effect of BTX-A intraperitoneal injection on visceral pain of rats and its effect on intestinal AChE and SP expression.Methods: 72 male adult Wistar rats were divided into four groups: BTX-A(2U,4U,and 6U) or vehicle(2ml) was injected intraperitoneally in B,C,D and A group.6 Rats of every group were challenged with acetic acid intraperitoneal injection after 1,4 and 8 weeks respectively.After abdominal writhing behaviors were monitored,the intestinal samples were immunohistochemical stained for AChE and SP examination.Results: At the end of one week,writhing test scores of rats in group C and D were decreased significantly compared with group A(P

BACKGROUND:Precision attachment has been widely used in complete denture because of its strong retention, good appearance, comfort and durability. Among them, magnetic attachment and bal-cap attachment are commonly applied for mandibular complete denture.
OBJECTIVE:To compare the clinical effects of magnetic attachment, bal-cap attachment and traditional clasp retainer used in the mandibular removable partial denture.
METHODS:A total of 45 patients with dentition defects were included in this study, they were treated with magnetic attachment, bal-cap attachment and traditional clasp retention of mandibular removable partial denture. Fifteen patients received one means. The satisfaction of patients and the periodontal health of abutments were observed after 3 years.
RESULTS AND CONCLUSION:Comparison of the patient satisfaction showed that, the aesthetics, retention conditions, masticatory function and comfort in magnetic attachment group were significantly better than traditional clasp retention group (P<0.05). The aesthetics and comfort in bal-cap attachment group were also better than traditional clasp retention group (P<0.05). There were significant differences on the retention conditions and comfort between magnetic attachment group and bal-cap attachment group (P<0.05). Gingival index was the highest in magnetic attachment group, then in bal-cap attachment group, and the lowest in traditional clasp retention group (P<0.05). The abutment tooth mobility degree was the lowest in magnetic attachment group, then in bal-cap attachment group, and the highest in traditional clasp retention group (P<0.05). The periodontal pocket depth in magnetic attachment group and bal-cap attachment group was lower than that in traditional clasp retention group (P<0.05). The alveolar bone height was the highest in magnetic attachment group, then in bal-cap attachment group, and the lowest in traditional clasp retention group (P<0.05). Experimental findings indicate that, precision attachment over-denture retention superior to traditional clasp removable partial denture in mandibular denture repairing. Magnetic attachment can provide better retention power and better protect the health of periodontal tissue compared with bal-cap attachment.

Objective To study the effects of internal and external biliary drainage on inducible nitric oxide synthase (iNOS) mRNA expression of Kupffer cells and serum tumor necrosis factor-alpha (TNF-α) in rats with obstructive jaundice. Methods Forty-eight male Sprague-Dawley rats were randomly assigned to obstructive jaundice (OJ), sham operation (SH), internal biliary drainage (ID) and external biliary drainage (ED) groups with 12 each. Kupffer cells were isolated by in situ hepatic perfusion and digestion with collagenase type Ⅳ, and purified by cell culture attachment. The expression of iNOS mRNA of Kupffer cells was detected by reverse transcription polymerase chain reaction (RT-PCR) and the serum TNF-α concentration was measured using ELISA method. Results The serum TNF-α level was increased in OJ group[ (110.84±26.3) pg/ml ]compared with SH group [-(88.4±17.9) pg/ml, (P=0.045)]. The TNF-α level in ID group[ (89.8±28. 3) pg/ml ]was significantly lower than that in ED group[ (118.64±22.7) pg/ml, (P=0. 011) ]and OJ group (P=0. 059). Expression of iNOS mRNA of Kupffer cells was significantly higher in OJ group (0. 824± 0. 24) compared with SH group (0. 384±0.35,P=0. 005). After relieving the OJ, the iNOS mRNA expression in ED group (0. 974± 0.48) was not suppressed (P=0. 321). On the contrary, the iNOS mRNA expression in ID group was suppressed and significantly lower than that in ED group (0. 59±0. 35) (P=0. 016). Conclusions Internal biliary drainage is superior to external drainage in terms of reversing the elevated serum TNF-α and in suppressing the iNOS mRNA expression of Kupffer cells in rats with obstructive jaundice.

Objective To determine the efficacy of endoscopic treatment for chronic pancreatitis(CP).Methods The clinical data of CP patients in our department from December of 2000 to March of 2009 were reviewed retrospectively.Results 77 patients had been successfully followed up.The short-term clinical symptom remission rate was 86.9% and the long-term 61.0%.9 patients underwent surgery after endoscopic treatment during the follow-up.The incidence of complications related to endoscopic treatment was 5.1% including post-ERCP(endoscopic retrograde cholangiopancreatography) pancreatitis,hemorrhage,cholangitis and perforation.No death related to endoscopic treatment occurred.Conclusion Endoscopic treatment for CP is safe and effective.

One kind of glycosaminoglycans (GAG) was extracted and purified from the ink of Sepiella maindroni de Rochebrune by using the procedures of trypsin and pronase digestion, ethanol precipitation and DEAE-cellulose inon-exchange chromatography. It was proved to be homogeneous by polyacrylamide gel and cellulose acetate membrane electrophoresis. No protein absorption was shown in UV spectrum, but typical absorption of GAG was shown in the IR spectrum. It was identified by paper and thin layer chromatography of the acid hydrolyzate that the GAG was composed of galactosamine, glucuronic acid and fucose. The percentage content of galactosamine, glucuronic acid, fucose, sulfate and protein in the GAG was 25. 6%, 29.2%, 23.3%, 8.2% and 3. 5%, respectively.

Objective To study the positions,features and sectional anatomy of the palatovaginal canal and vomerovaginal canal,comparing with CT and MRI findings. Methods The palatovaginal canals in 104 sides and vomerovaginal canals in 60 sides of dry skulls were observed.Three adult cephalic specimens were sliced into celloidin contiguous sections on coronal plane(0.5 mm in thickness) and the results were correlated with the CT studies of 11 patients and MR imaging of a cephalic specimen. Results In dry skulls,the palatovaginal canal displayed as complete canal in 79.8% of the cases,while appeared as groof in 15.4%.There was neither significant canal nor groof in 4.8%.The vomerovaginal canal demonstrated as complete canal in 36.7% of the speciments,while appeared as groof in 33.3%.This canal was communicated with the lateral palatovaginal canal in 15% and it was not detected in 15%.Four typical slices of the celloidin and 3 plane of CT and 3 plane of MRI were selected and the key structures were described.Conclusion The palatovaginal canal and vomerovaginal canal can be well depicted on coronal plane.

Objective To study a new endoscopic technique, double-balloon enteroscopy on its efficiency and indication of examining small intestinal diseases. Methods Thirteen patients suspected to suffer from small intestinal disease received double-balloon enteroscopy. By pumping the two balloons to grip the small intestinal walls, the enteroscope can be inserted further without forming redundant loops in the small intestine. Eight patients undergoing these procedures received a combination of sedative drugs and anisodam-ine intravenously. Results Nineteen procedures were performed in 13 patients, among them 8 patients were performed through anus and 11 patients through mouth. The enteroscope reached the 3th - 6th group small intestine through mouth or anus in average 80 minutes for each procedure. Eight positive lesions were detected or diagnosed by the enteroscopy and biopsy. The nausea, mild temporary abdominal pain and liquids effusing from the overtube occurred during examination through the mouth. There were no severe complications such as hemorrhage, perforation occurred. Conclusion The double-balloon enteroscopy is a new safe and controllable endoscopic procedure for those small intestinal disease patients without severe organ dysfunction and intestinal adhesion, and having the adventages of completing the procedure within 2-3 hours, allowing direct visualization of the entire small intestine, taking biopsy specimens to get pathological diagnosis, and is superior to barium enteroclysis.

Objective To improve the detection of G6PD heterozygotes in female patients by the optimum experimental factors of G6PD/6PGD specific value assay.Methods (1)Identifying the mutations of G6PD gene with the use of amplified refractory mutation system (ARMS).(2)Measuring the G6PD/6PGD specific value.Results According to the data analysed by statistics and ROC curve, the optimum experimental factors included that the incubation temperature was 37℃,the substrate concentrations were 0.78 mmol/L G6PNa2 and 0.195mmol/L NADP+, the reaction time was 10min.Conclusion The optimum experimental factors of G6PD/6PGD specific value assay may be used to improve the detection rate of G6PD heterozygotes in female patients.

The lack of medical ethics education among young doctors substantially hinders the construction of a harmonious physician-patient relationship under the current condition. This paper addresses the disharmonies and ethical conflicts in current physician-patient relationship, and sheds light on major factors related to the causes of the lack of medical ethics education among young doctors. Furthermore, special considerations and coping strategies to eliminate this dilemma are also provided.