Objective To evaluate the effect of gastric compound on patients with middle-late gastric cancer of spleen deficiency and stasis toxin. Methods Ninety patients with middle-late gastric cancer of spleen deficiency and stasis toxin were randomly divided into combined group, chemotherapy group, and gastric compound group, 30 cases in each group. Patients in the combined group were treated with gastric compound and chemotherapy;patients in the chemotherapy group were treated with placebo;patients in the gastric compound group were treated with gastric compound. The changes of QLQ-C30 scale integral, fatigue scale intergral, TCM symptom intergral, Karnofsky integral, and toxic and side effects of digestive tract and myelosuppression were observed to evaluate the effect of gastric compound on quality of life in patients. Results The changes of QLQ-C30 scale integral, fatigue scale intergral, TCM symptom intergral, Karnofsky intergal in combined group were better than those in chemotherapy group and gastric compound group, with statistical significance (P<0.05). The changes of fatigue scale intergral and TCM symptom intergral in gastric compound group were better than those in chemotherapy group, with statistical significance (P<0.05). The myelosuppression and toxic and side effects of digestive tract of combined group was lighter than those of chemotherapy group, with statistical significance (P<0.01). Conclusion Gastric compound combined with chemotherapy can improve quality of life in patients with middle-late gastric cancer of spleen deficiency and stasis toxin, and reduce myelosuppression and toxic and side effects of digestive tract.

Colorectal cancer (CRC) is a type of malignant tumor that seriously threatens human health and life, and its treatment has always been a difficulty and hotspot in research. Herein, this study for the first time reports that antipsychotic aripiprazole (Ari) against the proliferation of CRC cells both in vitro and in vivo, but with less damage in normal colon cells. Mechanistically, the results showed that 5-hydroxytryptamine 2B receptor (HTR2B) and its coupling protein G protein subunit alpha q (Gαq) were highly distributed in CRC cells. Ari had a strong affinity with HTR2B and inhibited HTR2B downstream signaling. Blockade of HTR2B signaling suppressed the growth of CRC cells, but HTR2B was not found to have independent anticancer activity. Interestingly, the binding of Gαq to HTR2B was decreased after Ari treatment. Knockdown of Gαq not only restricted CRC cell growth, but also directly affected the anti-CRC efficacy of Ari. Moreover, an interaction between Ari and Gαq was found in that the mutation at amino acid 190 of Gαq reduced the efficacy of Ari. Thus, these results confirm that Gαq coupled to HTR2B was a potential target of Ari in mediating CRC proliferation. Collectively, this study provides a novel effective strategy for CRC therapy and favorable evidence for promoting Ari as an anticancer agent.

Objective:To analyze the accuracy of digital orthognathic surgery by evaluating the effects of brackets, dentition status and registration method on the accuracy of registration of cone beam computed tomography(CBCT) dentition and laser scanning dental casts.Methods:A retrospective analysis of the data of patients who underwent digitally designed maxillary and mandibular surgeries from December 2017 to December 2019 in the Department of Orthognathic Surgery, Tianjin Stomatological Hospital. The CBCT and laser scanning dental mold data of the patients were imported into Mimics 20.0 software to build three-dimensional reconstructed dentition. Global registration and local registration of STL registration were used to register non-occlusal, occlusal CBCT dentition and laser scanning dental molds, which could be divided into four groups. Group 1: non-occlusal dentition using local registration, group 2: non-occlusal dentition using global registration, group 3: occlusal dentition using local registration, group 4: occlusal dentition using global registration. Each group included three subgroups, subgroup A used 3.0 mm distance parameters for registration once, subgroup B used 3.0 and 1.5 mm distance parameters for registration twice, and subgroup C used 3.0, 1.5 and 0.3 mm distance parameters for registration three times. With the assistance of the distance analysis function of Geomagic Studio software, the root mean square of the distance between the two images was obtained. The comparison of normal power data between subgroups was performed by analysis of variance, and the multiple comparisons among subgroups were performed by LSD- t test. After testing the normality and homogeneity of variance of the data, a general linear model was established to test the influence of bracket, dentition state and registration method on the accuracy of registration by multi-factor analysis of variance. Results:A total of 24 patients were enrolled, 7 males and 17 females, aged 19-30 years, 12 of which had orthodontic metal brackets and 12 had no brackets. (1) The local registration of each group is the best in subgroup A, and the global registration is the best in subgroup C. (2) The effects of bracket, dentition status and registration method on the registration result were statistically significant( P<0.05). The interaction among them was not significant ( P>0.05), but the interaction between bracket and dentition state, bracket and registration method, dentition status and registration method was significant ( P<0.05). Through simple effect analysis, it was found that in occlusal dentition registration/bracket registration, the simple single effect of registration method on registration accuracy did not exist ( P>0.05), while that of non-occlusal dentition registration/non-bracket registration had a simple single effect on registration accuracy ( P<0.05). Conclusions:If you use local registration, you only need to match the parameters of 3.0 mm once; if you use global registration, you need to register three times according to the parameters of 3.0, 1.5 and 0.3 mm. Bracket, dentition status, registration methods can all affect the accuracy of registration of CBCT dentition and laser scanning dental mold.

Objective:To analyze the accuracy of digital orthognathic surgery by evaluating the effects of brackets, dentition status and registration method on the accuracy of registration of cone beam computed tomography(CBCT) dentition and laser scanning dental casts.Methods:A retrospective analysis of the data of patients who underwent digitally designed maxillary and mandibular surgeries from December 2017 to December 2019 in the Department of Orthognathic Surgery, Tianjin Stomatological Hospital. The CBCT and laser scanning dental mold data of the patients were imported into Mimics 20.0 software to build three-dimensional reconstructed dentition. Global registration and local registration of STL registration were used to register non-occlusal, occlusal CBCT dentition and laser scanning dental molds, which could be divided into four groups. Group 1: non-occlusal dentition using local registration, group 2: non-occlusal dentition using global registration, group 3: occlusal dentition using local registration, group 4: occlusal dentition using global registration. Each group included three subgroups, subgroup A used 3.0 mm distance parameters for registration once, subgroup B used 3.0 and 1.5 mm distance parameters for registration twice, and subgroup C used 3.0, 1.5 and 0.3 mm distance parameters for registration three times. With the assistance of the distance analysis function of Geomagic Studio software, the root mean square of the distance between the two images was obtained. The comparison of normal power data between subgroups was performed by analysis of variance, and the multiple comparisons among subgroups were performed by LSD- t test. After testing the normality and homogeneity of variance of the data, a general linear model was established to test the influence of bracket, dentition state and registration method on the accuracy of registration by multi-factor analysis of variance. Results:A total of 24 patients were enrolled, 7 males and 17 females, aged 19-30 years, 12 of which had orthodontic metal brackets and 12 had no brackets. (1) The local registration of each group is the best in subgroup A, and the global registration is the best in subgroup C. (2) The effects of bracket, dentition status and registration method on the registration result were statistically significant( P<0.05). The interaction among them was not significant ( P>0.05), but the interaction between bracket and dentition state, bracket and registration method, dentition status and registration method was significant ( P<0.05). Through simple effect analysis, it was found that in occlusal dentition registration/bracket registration, the simple single effect of registration method on registration accuracy did not exist ( P>0.05), while that of non-occlusal dentition registration/non-bracket registration had a simple single effect on registration accuracy ( P<0.05). Conclusions:If you use local registration, you only need to match the parameters of 3.0 mm once; if you use global registration, you need to register three times according to the parameters of 3.0, 1.5 and 0.3 mm. Bracket, dentition status, registration methods can all affect the accuracy of registration of CBCT dentition and laser scanning dental mold.

We reported the discovery of six novel coumarins, toddasirins A-F (1-6), each endowed with modified isoprenyl or geranyl side chains, derived from the roots of Toddalia asiatica. Comprehensive structural elucidation was achieved through multispectroscopic analyses, single-crystal X-ray diffraction experiments, and advanced quantum mechanical electronic circular dichroism (ECD) calculations. Furthermore, the anti-inflammatory activity of these compounds was assessed. Notably, compounds 1-3 and 6 demonstrated notable inhibitory effects on nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW 264.7 cells, with 50% inhibitory concentration (IC₅₀) values of 3.22, 4.78, 8.90, and 4.31 μmol·L^-1, respectively.
Mice ; Animals ; Coumarins/chemistry* ; Rutaceae/chemistry* ; Anti-Inflammatory Agents/pharmacology* ; Plant Extracts/chemistry* ; RAW 264.7 Cells ; Nitric Oxide ; Molecular Structure