1.Establishment and Application of a Real-Time Quantitative PCR Assay for Detection of Campylobacter Jejuni in Human Feces
Yunpeng CHEN ; Wen LIN ; Jianping DENG
Journal of Modern Laboratory Medicine 2014;(5):85-88
Objective To establish a real-time quantitative PCR assay for the identification of Campylobacter jejuni in fecal samples.Methods Specific primers of the PCR were designed according to the conserved sequences of Campylobacterjeju-ni,and the real-time quantitative PCR assay was established.150 cases of fecal samples were tested by both culture and PCR methods.With the culture testing results as the reference standard,the sensitivity,specificity,accuracy and repetition of the real-time quantitative PCR were validated.Kappa test was used to estimate the difference between the two detection meth-ods.Results The standard carve of the real-time quantitative PCR assay fitted the equationY=-3.51Log(X)+37.09 (R2=0.996)well.The sensitivity,specificity,and accuracy of the established method were 92.4%,95.8% and 94%,respective-ly.The theoretical detection limit of the PCR method was 102 CFU/ml,and its reproducibility was good (CV<5%).Statisti-cal analysis demonstrated that the results of the two methods were consistent,and the consistent strength was very strong (Kappa=0.88,P<0.05).Conclusion The established real-time PCR method can assay the Campylobacterjejuni in human fecal samples rapidly and accurately.
2.Study on the Quinolone Antibiotic Resistance and Its Relative Genes in Campylobacter Jejuni
Yunpeng CHEN ; Wen LIN ; Jianping DENG
Journal of Modern Laboratory Medicine 2015;(2):27-29
Objective To establish a real-time fluorescence PCR method to detect the drug resistance genes of pathogenic Campylobacter jejunum in human stool samples,and investigate the relationship between quinoloneantibiotic resistance and the related genes in Campylobacter jejuni .Methods According to the gyrA and gyrB gene sequences that related with the fluoroquinolone resistance in Campylobacter jejuni ,the primers of the PCR method was designed and synthesized.A rapid real-time fluorescence PCR method to detect the drug resistance genes in Campylobacter jejuni samples was established,and the optimum reaction system and conditions were screened through an optimized approach.The developed method was com-pared with the classical drug susceptibility assay.Results It was found in the compared results that,there were 8 inconsis-tent strains of Campylobacter jejuni ,2 of the 8 strains were drug sensitive but contented the drug resistance gene,while 6 strains were drug resistant but had no drug resistant gene.Conclusion The established method can be applied to detect the drug resistance relative genes of gyrA and gyrB in Campylobacter jejuni .There was some correlation between the drug re-sistance representation and its genotype,but this point requires further studies.
3.Application of adjacent aesthetic unit combined liposuction in upper arm.
Meng DENG ; Yunpeng GU ; Hua LEI ; Zhenjun LIU ; Guie MA
Chinese Journal of Plastic Surgery 2014;30(6):439-442
OBJECTIVETo improve a better method to achieve the upper arm's circumferential harmony by using adjacent aesthetic unit combined upper arm liposuction.
METHODSLiposuction of upper arm, axilla and part of the scapula area was performed on 88 cases at one stage from January 2007 to March 2013.
RESULTSAll the cases achieve satisfactory results. The circumferential curve is smooth and elegant. No severe complication happened.
CONCLUSIONSThis method proved to be safe and effective and can be accomplished in one stage.
Adult ; Arm ; anatomy & histology ; surgery ; Axilla ; Esthetics ; Female ; Humans ; Lipectomy ; methods
4.Advances in Sichuan grass-root health information construction
Hu LONG ; Minghui SHEN ; Yunpeng MAO ; Ren DENG ; Changqi FENG
Chinese Journal of Medical Library and Information Science 2014;(3):4-8
After a description of the basic contents of Sichuan grass-root health information, health information systems at county level or below, and their roles and features, the key points of Sichuan grass-root health informa-tion construction program, progress and problems in Sichuan grass-root health information construction were stressed in discussion , with certain measures put forward for the solution of such problems .
5.Comparison of radiation doses between rotational angiography and standard angiography
Yunpeng DENG ; Huiliang LIU ; Zhigeng JIN ; Jianping LUO ; Shengli YANG ; Limin JING
Chinese Journal of Radiological Medicine and Protection 2014;34(7):547-550
Objective To compare radiation doses to patients between rotational angiography and standard angiography.Methods According to different modes,our experiment was divided into RA (Dual-axis Rotational Angiography) group and SA (Standard Angiography) group.The latter was divided into 3 subgroups (SA1,SA2 and SA3) in terms of different time of cine.The procedure time and the position were controlled by the order preset into the machine.Each group consisted of 10 repeated operations with a unique phantom to simulate the true patients.The radiation doses were measured by using dose meter provided by the machine and TLD system; the means of the data were compared.Results The mean of total accumulated DAP and AK in group RA were 3 061.6 mGy·cm2 and 64.2 mGy,respectively,compared with group SA1 (4 213.9 mGy·cm2 and 85.5 mGy),SA2 (6 436.0 mGy·cm2 and 112.2 mGy),SA3 (12 810.4 mGy·cm2 and 243.7 mGy).The mean of accumulated dose in group RA was 37.70 mGy,higher than group SA1 (26.56 mGy) but lower than group SA2 (46.86 mGy) and SA3 (77.79 mGy).The gap between highest dose and lowest dose in RA group was insignificant whereas significant in the subgroups of SA.Conclusions In comparison with conventional angiography,the dual-axis rotational angiography has the potential to reduce radiation exposure of patients significantly so as to prevent skin radiation injures.
6.R language-based analysis of big data about drugs prescribed in grass root clinics
Shuai WANG ; Xiaodong LIN ; Minghui SHEN ; Ren DENG ; Yunpeng MAO ; Changqi FENG ; Wen CHEN ; Hu LONG
Chinese Journal of Medical Library and Information Science 2015;(3):54-58
Objective To provide the evidence for health management decision-making and rational use of drugs grass root clinics by studying their drug prescription rules.Methods The prescribed drugs in clinics of 5 township health centers from September 2012 to September 2014 were retrieved from The Management Information System of Sichuan Grass Root Medical Institutions.Their big data were analyzed using R language.Results The commonly pre-scribed drugs in clinics were vitamin B6, vitamin C and cefixime tablets, which were usually used in combination. Conclusion Health administrative organizations can strengthen their supervision and management of prescribed drugs and promote their rational use in grass root clinics using unified management information system of grass root medical institutions in combination with information technology .
7.Medical and health organization management information system at grass-root level and platform at county level for data exchange in Sichuan Province:their design and implementation
Shuai WANG ; Xiaodong LIN ; Minghui SHEN ; Yunpeng MAO ; Ren DENG ; Wen CHEN ; Hu LONG
Chinese Journal of Medical Library and Information Science 2015;(9):12-16
The medical and health organization management information system at grass-root level and platform at county level for data exchange in Sichuan Province were designed and constructed according to the health information exchange service network in Sichuan Province and standard medical CDA file, in order to implement data exchange on the medical and health organization management information system at grass-root level and platform at county level, to insure the basic medical and health service for the public, and to improve their health level.
8.Application of third party testing in information system construction of Sichuan grass-root medical and health institutions
Minghui SHEN ; Changqi FENG ; Li FENG ; Yunpeng MAO ; Ren DENG ; Shuai WANG ; Jiefeng WU ; Xin YAO ; Zhihua YU
Chinese Journal of Medical Library and Information Science 2014;(3):15-18,22
After a description of the background and ideas to introduce the third party testing in information system construction of Sichuan grass-root medical and health institutions and the specific working contents of third party testing team, the problems to which importance should be attached in information system construction were analyzed with suggestions put forward for their solution .
9.Establishment and Application of Rationality Evaluation Criteria for Tumor Nutritional Standardized Treatment
Hai LIANG ; Juan SHEN ; Jun DENG ; Xiaoxia LI ; Yunpeng JI ; Jinlian ZHANG ; Yun LI ; Gang CHENG ; Cheng ZENG
China Pharmacy 2021;32(6):758-763
OBJECTIVE:To establish the eva luation criteria for the rationality of tumor nutritional standardized treatment ,and to provide reference for nutritional standardized treatment in tumer patients . METHODS :Based on domestic and foreign guidelines or expert consensus ,the rationality evaluation standard of tumor nutritional standardized treatmentwas formulated in our hospital (Bozhou Municipal People ’s Hospital ). 50 nutritional treatment medical records in our hospital from Jan. to Jun. 2019 were evaluated by weighted TOPSIS ;according to the evaluation results ,nutritional intervention was carried out ,and 50 nutritional treatment medical records (group B )from Aug. to Dec. 2019 were re-evaluated by the same method after intervention. RESULTS : The established evaluation criteria for the rationality of tumor nutritional standardized treatment in our hospital included 18 indicators,such as malnutrition diagnosis ,description of the nature of malnutrition ,nutrition screening and evaluation ,etc. After analysis ,the rational rate of nutritional treatment was only 18% in group A (Ci of ideal solution with 9 medical records≥0.6),and 78% in group B (Ci of ideal solution with59 medical records ≥0.6). There was statistical significance in the rationality of nutritional treatment before and after nutritional intervention (Ci≥0.6)(P<0.05). CONCLUSIONS :The established rational evaluation method of tumor nutritional standardized treatment is feasible ,and the evaluation results are intuitive and reasonable. Nutrition intervention is helpful to reduce the irrational rate of nutritional treatment.