Nanopublication is a novel semantic expression pattern.The relation between nanopublication and knowledge discovery was studied in terms of its emergence, improvement and progression in order to provide certain clues for discovering new knowledge.

Analyzed in this paper is the feasibility to carry out rural area-oriented remote medical education and to establish the remote medical education center by making use of the advantages in medical colleges and universities according to the medical and health situation and the requirement of remote medical education in rural areas, which was followed by some suggestions put forward for how to effectively run the remote medical education center with the poor medical conditions and few opportunities for rural medical staff to improve their medical care level taken into consideration.

Objective To investigate the effects of Mandarin speech rate on Mandarin acceptable noise level test in normal hearing adults.Methods Using the national Putonghua proficiency test material to produce 0.8 times,1.0 times and 1.2 times speed rate of ANL test materials.ANL tests were measured for 58 normal hearing a-dults using different peed matetrials.For each case,the most comfortable loudness(MCL)and background noise lev-el(BNL)were found.The ANL was then computed by subtracting the mean BNL from MCL.ResuIts Statistic anal-ysis indicated that there were no significant differences of the ANL(P>0.05)among the differenct speed materials, there were no correlation between MCL-ANL (P>0.05),negatively correlation between BNL-ANL(P<0.05)a-mong the differenct speed materials.ConcIusion Mandarin speech speed does not effect ANL test,the more the in-dividual’s ability to accept the background noise,the smaller the value of ANL.

Objective To study different test positions on vestibular evoked myogenic potentials (VEMPs) in youth ,and to find a suitable position and provide a guidance for clinical practice .Methods Thirty normal young vol-unteers were tested by vestibular evoked myogenic potentials ,using three different positions :supine with the head held straight up(SHU),supine with the head held up and turned away from the test ear(SHT),sitting with the head turned away from the test(SIT) ,the derivation rate ,latency and amplitude were analyzed .Results The deri-vation rate of SHU ,SHT and SIT were 100% ,100% and 63 .3% ,respectively .The derivation rate ,p13 ,n23 la-tency and p13 n23 inter-latency between SHU and SIT ,and between SHT and SIT had statistical differences (P<0 .05) .No statistical significant differences were found in derivation rate ,p13 ,n23 latency and p13n23 inter-latency between SHU and SHT (P>0 .05) .The amplitude was significantly different among the three positions (P<0 .05) . No statistical significant difference were found in derivation rate ,p13 ,n23 latency ,p13 n23 inter -latency and am-plitude between men and women of the three positions (P> 0 .05) .Conclusion The derivation rate of SHT was 100% with maximum amplitude .SHT is the most recommended position for clinical test in youth .The derivation rate of SHU was 100% ,and no statistical significant difference were found in p13 ,n23 latency and p13n23 inter-la-tency between SHU and SHT (P>0 .05) .SHU can be used in clinical test .SIT is not recommended for using in clinical test .Gender does not affect VEM Ps test .

Objective:To investigate the differences in the expression of microRNA (miR)-216a and its target gene SerpinB5 at the tissue level, and the effects of miR-216a on the proliferation of different liver cancer cells by regulating the expression of SerpinB5.Methods:Through bioinformatics prediction and selection of miR-216a that regulated SerpinB5. the expressions in liver cancer and normal tissues were detected by real time polymerase chain reaction (PCR). The miR-216a simulacrum and inhibitor, si-Serpinb5 and pcdna3.1-Serpinb5 to HepG2 and MHCC97H (97H) were transfected with liposomes, respectively. Real time PCR and Wester-Blot were used to detect the expression of miR-216a and SerpinB5 before and after transfection, and CCK8 was used to detect the influence of both on the proliferation of liver cancer cells.Results:The expression of miR-216a in human liver cancer tissues was higher than that in adjacent tissues, and the difference was statistically significant ( P < 0.01). The expression of SerpinB5 in human liver cancer tissues was lower than that adjacent tissues, and the difference was statistically significant ( P < 0.01). In HepG2 and 97H, miR-216a inhibitor and SerpinB5 overexpression group showed down-regulated miR-216a expression, which was statistically different from the control group ( P < 0.01). The proliferation of miR-216a inhibitor and pcdna3.1-serpinb5 group was lower than the control group, with statistically significant differences ( P < 0.01). Conclusions:The high expression of SerpinB5 can inhibit the proliferation of liver cancer cells, suggesting that SerpinB5 may have an anti-oncogene effect. MiR-216a may negatively regulate the expression of SerpinB5 and affect the proliferation of HCC cells.