Objective:This study is designed to analyze the relationship between different modes and the effect of bilingual teaching of Pathology in medical colleges for nationalities and improve the bilingual teaching efficiency of medical college for nationalities.Methods:We conducted a mode-control study to analyze the association between the three Chinese-English Teaching Modes [namely:Chinese(100%)Schoolteaching Mode with English(100%)multimedia courseware(Mode1),Chinese(100%)Schoolteaching Mode with Chinese-English multimedia courseware(Mode2),and Chinese(50%)and English(50%)Schoolteaching Mode with Chinese-English multimedia courseware(Mode 3)] and the levels of medical basic and medical English for pathology of the students(139),who had learned the course of pathology by ways of different bilingual teaching modes,from The Nursing-Speciality For Undergraduate Students(Majors In English),by Logistic Regression Analysis methods.Results:Compared with the individuals learning by Mode 1 or 2,the students who learned by Mode 3 possessed higher levels of medical basic and medical English for pathology(adjusted ORs=7.33 and 9.53,respectively,P

Objective:To investigate the best preservation conditions of N-terminal pro-brain natriuretic peptide( NT-proBNP) and provide detection references with stable performance for detection kits.Methods: ELISA was used to quantitatively detect the changes in NT-proBNP contents in various preservation solutions.The effects of basic buffer system, preservative Proclin300 and antibiotics on the preservation of NT-proBNP were analyzed using univariate analysis.The combination of various factors was then optimized using orthogonal experiments, to identify the best preservation system for NT-proBNP.Results: The univariate analysis determined that the basic buffer system for NT-proBNP was 0.02 mol/L phosphate buffered saline(PBS) at pH7.2,the addition of pre-servative Proclin300 could extend the preservation time of NT-proBNP at 37℃ by one day, the combined addition of penicillin and streptomycin prolonged the preservation time of NT-proBNP by one day compared with individually adding penicillin or streptomycin.The orthogonal experiments identified a preservation solution for NT-proBNP as 20%calf serum,1/1 000 Proclin300,120 U/ml penicillin and 80 U/ml streptomycin in a basic buffer system of 0.02 mol/L PBS at pH7.2.This solution was used to preserve an NT-proBNP reference sample at 37℃.Seven days later,the calibrated fixed-value of the sample at 37℃was only 1.3%lower than that at 4℃.Conclusion:Optimized NT-proBNP serum preservation solution could preserve NT-proBNP standard sample at 37℃ for seven days.

Objective To evaluate treatment and operation timing of anal fistula in acute leukemia patients.Methods Clinical data of 42 acute leukemia patients with anal fistula from 2010 to 2015 in First Hospital of Xinjiang Medical University were evaluated according to the choice of operation time.Patients were divided into two groups undergoing surgery before chemotherapy, (group A).and surgery after chemotherapy (group B).Clinical analysis was performed usingx2 or t test and correlation index.Results Neutrophil count in group B was lower than that in group A (x2 =8.077, P =0.004).Chemotherapy before surgery regime reduces the incidence of infection.Conclusions Chemotherapy before surgery may improve the prognosis of anorectal fistula patients of acut leukemia.

Objective To detect the effects of 17 β-estradiol(E2)on the expression of Calbindin-D9k (CaBP-9k) in pituitary GH3 cells,and to investigate the antagonistic effect of a selective estrogen receptor antagonist,ⅡCI 182780 on CaBP-9k expression.Methods A rat pituitary prolactinoma cell line,GH3 cell was used as the in vitro model.The localization of CaBP-9k in GH3 cells was observed by immunofluorescence.GH3 cells were cultured with exogenous E2-added medium for 24 hours,and the concentrations of E2 were 10-8,10-9,10-10M,respectively.mRNA and protein expression levels of CaBP-9k in different groups were analyzed by RT-PCR and Western blot analysis.The estrogen receptor antagonist,and ⅡCI 182780 was added to GH3 cells before E2 administration (10-8M)with the concentration of 10-6M,in order to investigate the regulation of ER-mediated pathway on the expression of CaBP-9k.Immunoprecipitation was used to detect the interaction between CaBP-9k and ERα.Results E2 had significant stimulatory effect on the CaBP-9k expression of GH3 cells in a dose dependent manner,and the expression level of CaBP-9k was higher when treated with a higher concentration of E2.ⅡCI 182780 could suppress the stimulatory effect of E2 on the CaBP-9k expression of GH3 cells.The expression level of CaBP-9k was significantly reduced by coadministration of E2 with ⅡCI 182780 in GH3 cells,which meant the CaBP-9k expression was mediated through ERα pathway.The immunoprecipitation results further illustrated the fact that CaBP-9k could directly interact with ERα,and E2 could increase the interaction between CaBP-9k and ERα.Conclusion Estrogen might induce CaBP-9k expression via ERα mediated pathway and CaBP-9k could directly combine with ERα,suggesting that CaBP-9k might be involved in the biological effects mediated by ER pathway in GH3 cells.

Objective:To investigate the role of Akt signaling pathway in the regulation of breast cancer bone metastasis induced pain behavior in rat.Methods:Model rats were randomly divided into three groups:Model group,Model+GSK690693 group and Model+ Saline group.On PID 13,14 and 21,Model+GSK690693 group rats were intrathecally injected with GSK690693,a specific inhibitor of Akt.Model+Saline group were injected with saline instead.The pain related behaviors were respectively recorded on PID 0,7,14 and 21.The expression ofp-Akt in DRG used for western bloting were examed on PID 21.Results:After the injection of Akt specific inhibitor GSK690693 by intrathecal,In the Model+GSK690693 group,the threshold value of mechanical contraction reflex was increased,the spontaneous pain behavior and the expression of p-Akt in DRG decreased.On PID 14 d and 21 d,the pain behavior of rats in Model+GSK690693 group was significantly different from that of Model+Saline group and Model group (P＜0.01);On PID 21 d,There was significant statistical significance (P＜0.01) on the expression of p-Akt and Model in the ipsilateral Model+GSK690693 of DRG group,which was statistically significant (P＜0.05) compared with the Model+Saline group.Conclusion:Intrathecal injection of Akt specific inhibitor GSK690693 inhibits rat pain related behaviors induced by bone metastasis in rat breast cancer.

Objective: To explore the association between excite seeking personality and alcohol use, and provide reference basis for intervention measures of drinking behavior. Methods: Totally 5966 college students in 8universities [2180 males, mean age (20 ± 1) ] were selected from Hunan province by using multi-stage stratified cluster random sampling method. We Chat-based anonymous questionnaire survey was conducted by using general questionnaire, excite-seeking personality scales, alcohol questionnaire, and alcohol use disorders identification test. Results: The rate of 12-month alcohol drinking, binge drinking, hazardous and harmful drinking among college students were 47. 5％, 24. 5％, 7. 4％, respectively. High degree of excite seeking personality had more likelihood to having had alcohol drinking, binge drinking, hazardous and harmful drinking (Ps ＜ 0. 01). Excite seeking personality was an independent risk factor of 12-month alcohol drinking, binge drinking, hazardous and harmful drinking. Conclusion: It suggests that excite-seeking personality may be a related factor of alcohol drinking behavior.

Objective To evaluate the reliability and validity of the Chinese-version of the scale for the assessment of illness behavior(SAIB) in Chinese medical college students. Methods In view of the professional and cultural basis of the translation of SAIB,723 medical students were asked to complete the SAIB and the Illness Attitude Scale. After two weeks,75 students were followed to complete the SAIB. Re-sults After the analysis of the scale project analysis and the exploratory factor analysis,23 entries were re-tained and five common factors were extracted( verification of diagnosis,expression of symptoms,medication/treatment,consequences of illness,somatic symptom scanning),and accounted for 56.14％ of the total vari-ance.The confirmatory factor analysis identified a five factors model(SRMR=0.057,χ2/df=1.80,SRMR=0.06,RMSEA=0.05,GFI=0.912,CFI=0.93,TLI=0.91,IFI=0.93,PGFI=0.72,AGFI=0.89).The correla-tion validity between SAIB scale total score and treatment experience and symptoms affected in IAS scale were -0.31,-0.36 (P<0.01).Reliability coefficients of the five dimensions and total score ranged from 0.61 to 0.88,and the test-retest reliability was 0.78. Conclusion The Chinese version of the scale for the assess-ment of illness behavior is a reliable and valid instrument in Chinese.

Objective To explore the reliability and validity of the preliminary revision of the Philadelphia Mindfulness Scale (PHLMS) in Chinese undergraduates.Methods Considering the differences in the cultural and linguistic,the PHLMS was translated and used to conduct a questionnaire survey among 1 221 undergraduates.All participants were also surveyed with five facets mindfulness questionnaire (FFMQ),the white bear suppression inventory (WBSI) and acceptance and action questionnaire-2rd edition (AAQ-Ⅱ) as conduct reliability and validity test.Results The exploratory factor analysis showed that PHLMS could extract two factors,and the cumulative variance contribution rate was 40.355％.The confirmatory factor analysis indicated good fit (x2/df=2.781,CFI =0.945,NFI =0.884,IFI =0.922,TLI =0.906,CFI =0.922,RMSEA =0.053).The internal consistency reliability of the awareness subscales was 0.785,and the internal consistency reliability of the acceptance subscales was 0.772.The correlation coefficients among the PHLMS and FFMQ was significant(r=0.398,P＜0.01).The acceptance subscales were significantly negatively correlated with WBSI and AAQ-Ⅱ(r=-0.458,-0.288,P＜0.01).Conclusion The validity and reliability of the Chinese version of PHLMS meet the measurement criterion.

Objective To evaluate safety and efficacy of endoscopy therapy for early colorectal carcinoma of different types. Methods Clinical data of 113 patients with early colorectal carcinoma who were treated with different endoscopic therapies including polypectomy,EMR and ESD(ESD with snare and standard ESD)were retrospectively analyzed. The size, en bloc resection rate, curative resection rate, procedure time,associated complications and recurrence rate were compared among groups. Results Eleven pedunculated lesions were treated with polypectomy or EMR, which were en bloc resected and curative resected with no complication or local recurrence. Nineteen semi-pedunculated lesions were treated with EMR or ESD with no complications. Lesions treated with EMR were smaller than those of ESD(P=0.026), and had a lower en bloc resection rate[77.8%(14/18)VS 1/1,P=1.000]. The difference of curative resection rate and recurrence rate between groups was not significant(P>0.05). Eighty-three sessile lesions were treated with all three procedures.Lesions treated with ESD were larger than EMR(P=0.000),with a higher curative resection rate than EMR[95.5%(42/44)VS 77.8%(28/36),P=0.041]and a longer procedure time than EMR(P=0.000). There were no significant difference in severe complications[9.1%(4/44)VS 0],en bloc resection rates and recurrence rates(P>0.05). Ten lesions treated with ESD-S had a medium size between EMR and standard ESD group, with a significant shorter procedure time than standard ESD group(36.9 ± 24.7 min VS 120.4 ± 152.3 min, P=0.004).They were en bloc resected and complete resected. Conclusion Endoscopic treatment is safe and effective for early stage colorectal carcinoma. In order to get en bloc curative resection,endoscopists should carefully choose a suitable technique based on the macroscopic morphology of lesions. ESD-S can be used as a transitional method from EMR to ESD.

Objective To assess the efficiency and safety of endoscopic submucosal dissection (ESD)on treatment of colorectal lesions. Methods Clinical data of 163 patients with colorectal lesions, who underwent ESD at Endoscopy Center of Peking University First Hospital from June 2012 to June 2016, were retrospectively analyzed. The clinicopathologic features, the rate of en bloc resection, complete resection,complication and recurrence were reviewed. Results Among the 163 colorectal lesions,118 were mucosal lesions and 45 were submucosal ones. The mucosal lesions included 31 cases of protruding type,22 of flat type and 65 of laterally spreading tumor(LST). The submucosal lesions were all protruding type. The median lesion diameter was 2.2(2.0)cm, median operation time was 42(53)min. En bloc resection rate was 92.6%(151/163),and complete resection rate was 86.5%(141/163). The risk factor related to en bloc resection rate and complete resection rate was tumor size(P<0.05).Complications occurred in 4 cases, including 2 cases of intraoperative bleeding and 1 case of delayed bleeding who received endoscopic hemostasis therapy,and 1 patient with delayed perforation,which was managed by emergency surgery. Four recurrences were observed in 135 cases during follow-up, of which 3 were cured by additional endoscopic treatment,and 1 by surgery. Conclusion ESD is a safe and effective, but less invasive technique for colorectal lesions. Favorable long-term clinical outcomes can be achieved when en bloc curative resection is performed.