Objective To investigate the curative effects of the modified coloclysis method on con-stipation in gerontal patients. Methods 80 cases of gerontal patients with constipation were divided into the observation group and the control group with 40 cases in each group.The observation group was treated with modified coloclysis method and traditional coloclysis method was used in the control group. The dura-tion of liquid retention time in enteric cavity, the times of defecation after coloclysis and relapse rate of constipation were recorded and compared. Results The retention time of liquid in enteric cavity was longer, the times of defecation after coleclysis was less and relapse rate of constipation was lower in the ob-servation group than those in the control group. Conclusions The modified coloclysis method to treat gerontal constipation is better than the traditional coleclysis method.

Objective To explore the integrity of white matter changes in patients with vascular mild cognitive impairment (VaMCI)based Leukoaraiosis.Methods The patients which appeared white matter hyperintensities in subcortical were performed psychological testing,then 48 VaMCI patients and 18 without cognitive impairment volunteers(NC)were choosed and performed DTI scanning,to determine the white matter’s values of FA and ADC in the following bilaterally symmetrical regions of interest(ROI):entorhinal cortex lobe,hippocampal vault,anterior of corpus callosum,posterior of corpus callosum,inferior frontal lobe,superior temporal lobe and angular gyrus of occipital lobe. Then the differences of the data of DTI between two groups and the relevance between neuropsychological score and DTI’s data were analysed.Results ADC values were significantly higher of VaMCI group in the left inferior frontal lobe,left anterior of corpus callo-sum,left hippocampus vault and right angular gyrus compared with NC group(P <0.05),and in the left hippocampus ADC values and MoCA scores was negatively correlated(r=-0.34,P =0.02).There was no significant difference of FA values between groups. Conclusion The ADC values in many cognitive related areas was reduced in VaMCI patients.

70%. The cluster analysis and factorial analysis showed that the fierceness, cruelty, and vital qi damaging were often clustered with each other and constituted the factors of main characteristics of Du-pathogen. In the Logistic regression analysis, it was discovered that fierceness, cruelty, and multi-damaging were closely related with the recognition of "the excessive pathogen damaging the health" (P

Objective To investigate the effect of acupoint massage on blood glucose in type 2 diabetic patients. Methods A total of 67 patients with type 2 diabetes mellitus patients, using the random number table method were divided into experimental group of 34 cases and 33 cases in the control group by random number table method. The experimental group was given five groups of acupoint massage and "five carriages" management to diabetes patients (control diet, exercise, blood sugar and lipid lowering drugs, diabetes knowledge propaganda and education, blood glucose monitoring). The control group was administered only the "five carriages" of diabetes. The two groups were observed for 2 months, and were compared the changes of biochemical indexes of glucose metabolism. Results After 2 months, the experimental group's fasting plasma glucose (FBG), 2 hours postprandial blood glucose (2 hBG), and glycated hemoglobin (HbA1c) decreased from the original (10.12±4.15) mmol/L, (15.32±5.65) mmol/L, and (9.36 ± 2.78)% to (6.04 ± 1.75) mmol/L, (8.42 ± 3.01) mmol/L, (6.35 ± 0.78) % , respectively, there were statistically significant differences within the group (t=5.28, 6.28, 6.08 respectively, all P<0.01). After 2 months, the FBG, 2 hBG, and HbA1c in the control group decreased from (11.23±6.02) mmol/L, (16.31± 6.23) mmol/L, and (9.67 ± 2.59) % to (7.12 ± 1.21) mmol/L, (9.58 ± 2.68) mmol/L, and (7.47 ± 1.96) % , respectively, and the differences within the group were statistically significant (t = 3.90, 5.79, and 3.95, respectively, all P<0.01). After 2 months, there was a significant difference between the FBG, 2 hBG, and HbA1c groups in the experimental group and the control group (t=2.96, 1.69, 3.09, P<0.05). There was no significant difference in the FBG and 2 hBG groups after 1 week of intervention in the experimental group (P>0.05). Conclusions To acupoint massage with basic intervention for type 2 diabetes patients can effectively consolidate the hypoglycemic effect, which is worthy of clinical reference and promotion.

AIM: To observe the dynamic alteration of myristoylated alanine-rich C kinase substrate(MARCKS) mRNA expression in rat hippocampus with acute multi-cerebral infarction,and discuss the relationship between the alteration of hippocampus MARCKS gene and ischemia damage.METHODS: The acute multi-cerebral infarction model was established by method of Kaneko.Neurological function deficits were evaluated in the behavior test.The consequences of cerebral ischemic damage were examined by histopathological analyses.The MARCKS mRNA expression was measured by semi-quantitative PCR.RESULTS: The rats in acute multi-cerebral infarction group showed different level changes of neurological function deficits.The hippocampus damage of histopathology became significant 24h after ischemia.At the same time,the MARCKS mRNA expression was upregulated at the area of rats hippocampus during ischemia,and its overexpression started 1h after ischemia,and reached maximum7d after ischemia.CONCLUSION: MARCKS mRNA of rat hippocampus overexpresses during acute cerebral ischemia.This MARCKS mRNA overexpression is related with hippocampus ischemia damage.

Improper disposal of herb residues in China has caused severe problems to the surrounding environment and human safety. Three herb residues, i.e., compound Kushen injection residues (CKI) and part one and part two of Qizhitongluo Capsule residues (QC1 and QC2, respectively), were used for the cultivation of Pleurotus ostreatus. The effect of the supplementation of corncobs (CC) with different herb residues on yield, nutritional composition, and antioxidant activity of P. ostreatus was investigated. Compared to the control, the higher mycelial growth rate was observed on substrates CC +30% CKI and CC +30% QC1, while the higher yield was obtained from substrates CC +30% QC2 and CC +30% CKI. Moreover, chemical analysis of fruit bodies revealed that the addition of herb residues to CC significantly increased proteins, amino acids, ashes, minerals (Na and Ca), and total phenolic contents but significantly reduced carbohydrates and IC50 values of DPPH radicals. In addition, no heavy metals (Pb, Cd, and As) were detected in the fruiting bodies harvested from different substrate combinations. These results demonstrated that mixtures of CC with herb residues might be utilized as a novel, practical, and easily available substrate for the cultivation of P. ostreatus, which is beneficial for the effective management of herb residues.
Amino Acids ; Carbohydrates ; China ; Fruit ; Humans ; Inhibitory Concentration 50 ; Metals, Heavy ; Minerals ; Miners ; Phenol ; Pleurotus*

Objective:To establish a diagnostic system for heat toxin syndrome of acute cere-bral infarction.Based on this toxin syndrome diagnostic system,the general principles of heat toxin development will be uncovered,and the critical turning point at which the heat toxin syndrome occurs will also be explored.Methods:In this study,a total of 271 hypertension patients with cerebral infarction within 72 h were recruited from the Affiliated Dongfang Hospital of the Beijing University of Chinese Med-icine,the Affiliated Dongzhimen Hospital of Beijing University of Chinese Medicine,the Affil-iated Renmin Hospital of Peking University,the Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,the Affiliated Hospital of Shandong University of Traditional Chinese Medicine,the Affiliated Hospital of Changchun University of Traditional Chinese Medicine,the Affiliated Hospital of Hebei University of Traditional Chinese Medicine and China Meitan General Hospital from August,2008,to December,2009.The patients' Chi-nese medical information was recorded on days 1,3,5,7,and 14 during their hospitalizations.The medical records were recorded according to traditional Chinese medicine (TCM) theory and included the serum marker levels at the beginning and at the end of the trial.The time line was also analyzed.Results:The level of Hs-CRP,PAG,NSE,OX-LDL,and MMP-9 were abnormal and,were higher in CI patients compared to hypertension patients.In the study of the heat toxin diagnosis system,according to the entropy clustering results,30 combinations of the medical information can be sorted into the traditional syndromes,but 13 combinations cannot be sorted.To obtain more precise symptoms related to the heat toxins,a logistic regression equation was set up with the variables from the unsorted medical information;the dependent variables were fever and BP fluctuation.Weighted variables were obtained.MLP analysis demonstrated that the diagnosis model was stable and precise.The accuracy reached 83.82％.The ROC test showed that seven points of the diagnosis system was the best cutting point,with a sensitivity of 0.857 and a spec-ificity of 0.955.Progressing stroke was related to heat toxin syndrome.When the turning point appeared,the combination of symptoms,such as coma,aphasia,gummy eyes,and halitosis,predicted the deterioration or recovery of Cl.The heat toxin syndrome existed in every sub-type of Cl;however,the observed heat toxin levels were highest in PACl and lowest in LACl.Meanwhile,blood and sputum stasis syndromes transformed into heat toxicity were one source of heat toxin syndrome.Conclusion:Heat toxin syndrome,as well as qi/blood/sputum stasis,co-existed in the CI pa-tients,and the transformation frequently appeared during the process.Three to five days after the onset of Cl was the turning point,at which time several combinations of medical indicators make it possible to predict the development of Cl.

Objective To investigate the correlation between waist hip ratio (WHR) and carotid atherosclerosis and TCM pattern manifestations in women aged over 40 with high risk of stroke. Methods The perimenopausal population with high risk of stroke (n=629) was chosen and divided, according to quartile of WHR,into 4 groups, and general clinical data and incidence of carotid atherosclerosis were compared among 4 groups. Taken group I as reference, risk of occurring carotid atherosclerosis was analyzed in other 3 group. The correlation between WHR and incidence of carotid atherosclerosis was studied by using binary Logistic regression analysis. Meanwhile, the population was divided into menopausal group and non-menopausal group, and WHR, incidence of carotid atherosclerosis, TCM symptoms and features of tongue and pulse were compared between 2 groups. Results The indexes of age, family history of stroke, menopausal cases, body mass index (BMI), overweight cases, triglyceride (TG) and high-density lipoprotein (HDL) had significant difference among 4 groups (P<0.05). The incidence of carotid atherosclerosis increased successively in 4 groups and difference had statistical significance (P<0.01). Compared with group I,risk of occurring carotid atherosclerosis was by 1.348 times,1.927 times and 2.305 times in other 3 groups(P<0.01). WHR was correlated to incidence of carotid atherosclerosis after controlled other variables (P <0.01). WHR was higher (P <0.05) and incidence of carotid atherosclerosis increased significantly (P <0.01) in menopausal group compared with non-menopausal group. The TCM pattern manifestations of qi deficiency, yin deficiency, yang deficiency and blood stasis were significantly more in menopausal group than those in non-menopausal group (P<0.05 or P<0.01). The pattern identification of viscera and bowels involved liver, spleen and kidney,and disease nature showed deficiency complicated by excessiveness and deficiency was the basis. Conclusion The risk of carotid atherosclerosis will be higher when WHR increases in women aged over 40 with high risk of stroke. WHR can be taken as an indicator of carotid atherosclerosis. The treatment with TCM is based on pattern identification of liver, spleen and kidney, and disease nature shows deficiency complicated by excessiveness and deficiency is the basis.

OBJECTIVETo study apoptosis-regulating cytokines and apoptosis on focal cerebral ischemia and reperfusion in rats treated with Xinnao Shutong capsule.

METHODRat models of focal cerebral ischemia and reperfusion were established by thread ligation in middle cerebral artery occlusions (MCAO). After 24 hours, the brains were removed to detect changes of protein expression of Bax, Bcl-2, Fas, Fas-L and caspase-3 by immuno-hisochemistry, and apoptosis of cortical neurons by TUNEL RESULT: Compared to control, brain cortex have decreasing the protein expression of Bcl-2 and the ratio of Bcl-2/Bax, increasing the protein expression of Bax, Fas, Fas-L and caspase-3 of ischemia and reperfusion models group (P < 0.01). Xinnao Shutong capsule group could increase the protein expression of Bcl-2 and the ratio of Bcl-2/Bax, and obviously decrease the protein expression of Bax, Fas, Fas-L and caspase-3, then reduce the number of apoptotic cells of cortex (P < 0.01).

CONCLUSIONXinnao Shutong capsule protect injured rat brain tissue, may be related to decrease neuronal apoptosis and adjusted protein expression of apoptosis-regulating cytokines.

Animals ; Apoptosis ; drug effects ; Apoptosis Regulatory Proteins ; genetics ; metabolism ; Brain Ischemia ; drug therapy ; metabolism ; surgery ; Capsules ; administration & dosage ; Cerebral Infarction ; drug therapy ; metabolism ; surgery ; Disease Models, Animal ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Gene Expression ; drug effects ; Humans ; Male ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Reperfusion

Objective: To provide guidelines for the prevention of post-stroke dementia, we investigated whether stroke patients were aware of post-stroke dementia and their level of dementia-related knowledge. Methods: Five dementia-related questions were designed. A field survey was conducted in a question-and-answer method using the designed questions. The surveys assessed 3000 stroke patients (2 weeks-6 months after stroke attacks) from 14 hospitals/clinical centers in 7 provinces and cities across China. Results: Among 3000 stroke patients, 80.5％had heard of dementia, 39.1％knew that stroke can lead to dementia, 55.7％ believed dementia can be prevented, 50.8％ thought dementia can be cured, and only 8.8％had ever seen a doctor because of memory deterioration. Then, patients were classified into three groups, including a no cognitive impairment (NCI) group, a mild cognitive impairment (MCI) group, and a mild dementia (MD) group. Among the MCI and MD groups, only 8.7％ (75/861) and 9.9％ (64/649) of patients, respectively, had ever seen a doctor because of memory deterioration. According to our results, patients with a higher level of cognitive impairment had a lower awareness of dementia (P<.001). Conclusion: The awareness of dementia in stroke patients in China is low, and the consultation rate is even lower. Moreover, patients with a higher level of cognitive impairment have a lower awareness of dementia. To improve public awareness and improve prevention, more emphasis should be put on ed-ucation regarding post-stroke dementia. Routine cognitive function screening should be conducted on stroke patients as an effective way to assess dementia.