Objective To observe the effect of basic fibroblast growth factor (bFGF) gene therapy on astrocytes glial fibrillary acidic protein (glial fibrillary acidic protein,GFAP) and vimentin (Vimentin,VIM) protein expression of astrocytes in rats with whole brain radiation brain injury (RIB) in order to provide an experimental basis for exploring new ways to treat RIB.Methods Sprague-Dawley rats were grouped and received single 25Gy for whole brain irradiation to established brain radiation injury (radiation injuries of the brain,RIB) model,bFGF gene therapy groups were given intracerebroventricular injection of bFGF-pcDNA3.1 (±) plasmid and set non-irradiated group as control.Before irradiation and post-irradiation 20 d and 60 d,respectively,GFAP and VIM expression were observed in each group of brain tissue.Results Radiation group with pathological examination showed mild degeneration of hippocampal and cortical neurons,and white matter regions presented the organizational structure comb loose and perivascular space enlargement compared with the control group.But the bFGF treatment group was significantly lighter.The expression of GFAP were increased in each group after radiation.GFAP positive cells of bFGF treatment group (65 ±6.2) were higher than that of irradiation group (49 ±5.8) and control group (18 ± 2.4) (P ＜ 0.05) at 20 d.GFAP positive cells at 60 d in bFGF treatment group (44 ± 5.1) were significantly reduced (P ＜ 0.05) than at 20 d and there were no significant difference (P ＞ 0.05) in the other groups between 20 d and 60 d.VIM expression of bFGF treatment group was higher at 20 d (0.94 ±0.12) compared to irradiated group (1.45 ± 0.26) and no significant difference of VIM expression was found in each groups at 60 d.Conclusion Irradiation with 25Gy-ray can increase the expression of GFAP and VIM in rats brain at acute phase,bFGF gene therapy can increase the expression of GFAP and decrease the expression of VIM.

Objective To observe the effects of exogenous basic fibroblast growth factor (bFGF) on radiation-induced apoptosis of C17.2 neural stem cells (NSCs) with γ-secretase inhibitor (DAPT) condition and explore the relationship between bFGF and Notch signal pathway.Methods The cell viability was detected by using the MTF method.After the cells attached to the flasks,different concentrations of DAPT was added in accordance with the experimental design and cultured cells for 24 h.C17.2 NSCs were subjected to irradiation exposure by linear accelerator and treated with bFGF (40 ng/ml) 5 min after the exposure.After 48 hours,the apoptosis of cells was detected by using Flow Cytometry.Results After adding in DAPT,the cells growth was inhibited and depended on the concentrations of DAPT.Compared with the control group,all groups had statistically significant differences(P＜0.05).Flow cytometry showed compared with the control group all groups had significant differences (P＜0.05).The apoptosis rate was (11.53±0.81)％ in radiation group,(7.18±0.0.94)％ in radiation+bFGF group,(9.82±0.77) ％ in DAPT group,(21.45±0.98) ％ in Radiation+DAPT group and (10.26+ 1.03) ％ in Radiation+ DAPT +bFGF group.Between Radiation + bFGF group and Radiation group,it had statistically significant difference(P＜0.05).The pairwise comparisons of DAPT group and Radiation + DAPT group which had the same DAPT concentration had statistically significant differences (P＜0.05).The pairwise comparisons of Radiation + DAPT+bFGF group and Radiation + DAPT group which had the same DAPT concentration had statistically significant difference(P＜0.05).Conclusion Fx ogenous bFGF can inhibit apoptosis of C17.2 NSCs.Notch signaling patbway inhibitor DAPT can promote apoptosis of C17.2 NSCs which are subjected to irradiation exposure by linear accelerator and bFGF can weak apoptosis.bFGF protective effect on radiation-induced neural stem cells may be related to the Notch signaling pathway.

BACKGROUND:The studies addressing osteoporosis in male starts relatively late,which lack effective and acknowledged method in treating.Epimedium,a Chinese medicine can be used in treating with osteoporosis for its obvious regulation of immune system,skeleton and reproductive system.OBJECTIVE:To investigate the effects of epimedium on bone mineral density(BMD) and bone structure performance in male castrated rats.DESIGN,TIME AND SETTING:The randomized controlled animal study was performed at the Geriatric Disease Laboratory of Tongji Medical College,Huazhong University of Science and Technology from January 2007 to April 2008.MATERIALS:Fifty adult,healthy,male,Sprague Dawley rats,weighing 340?10 g,with 15-week-old,were selected to prepare osteoporosis models.METHODS:Fifty rats were assigned to the control,model and three treated groups,with 10 animals in each group.The rats in the treated groups were treated by epimedium with different doses at 2 weeks after models preparation for 12 weeks.The doses were 1.0(low dose),5.0(medium dose),10.0(high dose) g/(kg?d) respectively.The animals in the other group were treated by aequales sodium chloride.MAIN OUTCOME MEASURES:Biochemical markers,BMD and bone morphometry of rats the fourth lumbar vertebra was evaluated after damaged by self-made SL22000 bone fatigue system.RESULTS:The BMD in the control,medium high dose groups were higher than that of the model group(P

ObjectiveTo study the effects of dl-3n-butylphthalide on study and memory abilities and hippocampus expression of MMP-9 in rat model of radiation injuries of brain.MethodsWistar rats were divided into radiation group radiated by linear accelerator and control group.Morris water test were taken to study the learning and memory ability of rats in each group before irradiation and 120 d after irradiation.ResultsMorris water maze test results showed that:in the academic test,compared with model group,high-dose butylphthalide group and low dose butylphthalide group had significantly shorter escape latency( (79.61 ± 7.17 )s,(43.64 ± 4.72)s,(42.15 ±4.19 ) s) and decreased number of errors ( 36.43 ± 6.59,14.58 ± 4.22,13.66 ± 3.91 ) (P ＜ 0.01 ).In the memory test,compared with model group,high-dose butylphthalide group and low dose butylphthalide group had significantly longer the former platform quadrant time respectively (30.09 ± 3.68 ) s,( 31.25 ± 3.17 ) s vs ( 17.57 ±4.29) s and faster swimming speed respectively (P ＜ 0.01 ).ConclusionButylphthalide can significantly reduce hippocampal neurons expression of MMP-9,which indicates that butylphthalide has curative effect on radiation brain injuries by regulating the expression of MMD-9.

Objective To investigate the clinicopathologic features of cerebral hemisphere ulegyria in children with refractory epilepsy.Methods The clinical and pathologic findings were reviewed in 26 children who underwent resection of lesion and epileptogenic lobectomy operation in the Neurosurgery Department of Haidian Hospital,Haidian District of Peking University Third Hospital,from January 2011 to August 2015,and pathological diagnosis was cerebral hemisphere ulegyria.Results All children including 19 male and 7 female had medically intractable seizures.The mean ages of seizure onset and disease duration were 3.93 years old (from 10 days to 12 years old) and 5.42 years (from 1 month to 13 years),respectively.Eight cases had seizures because of perinatal period injury,and 12 cases developed seizures owing to infancy injury and others had no cause of disease.The mean operation age was 9.35 years old (5-14 years old).Fourteen cases underwent multilobar resection and the whole corpus callosum incision,and 12 cases were given modified anatomical cerebral hemisphere resection.The pathological diagnoses of brain tissues were cerebral hemisphere ulegyria with focal cortical dysplasia(FCD) Ⅲ d and dual pathology.Seizure outcome after the operation revealed that 19 cases (73.08％) had an Engel grade Ⅰ,3 cases (11.54％)had an Engel grade Ⅱ,2 cases (7.69％) had an Engel grade Ⅲ,and 2 cases (7.69％) missed the follow-up.Conclusions Acquired brain injury during the period of infant can cause ulegyria and cortical dysplasia,resulting in intractable epilepsy.The treatment of improved anatomical cerebral hemisphere resection and multilobar resection is significantly effective.

Objective To investigate the effect of 3-n-butylphthalide (NBP) on learning and memory abilities in vascular demensia (VD) rats. Methods VD model rats was established by two-vessel method. 60 3-month-old Wistar male rats were randomly divided into VD group, sham-operate group and NBP group. Rats in NBP group were given NBP 120 mg · kg-1 · d-1 ,VD and sham-operate group were given equal quality vegetable oil.Morris water maze was used to assess the learning and memory abilities in each group and HE staining was used to observe the hippocampus morphology of the rats. Results The escape latency in hidden plat test was( (38.34 ±2.46 ) s, ( 14.83 ± 3.77s ), ( 75.74 ± 6.33 ) s ) and the original platform quadrant time was ( ( 26.45 ± 4.66 ) s,(35.21 ±3.78)s, ( 18.67 ±5.39)s) in NBP group,sham-operate group and VD group respectively. Compared to VD group, NBP group had obviously decreased escape latency in hidden plat test, increased original platform quadrant time and distinctly decreased the necrosis of the neurons in HE dyeing. Conclusion NBP can improve the learning and memory deficits in VD rats.

OBJECTIVE To study the characteristic of nosocomial pulmonary infection to find out the measures for prevention and control.METHODS The patients with radiation brain injuries(RIB) from Oct 2004 to Jun 2007 underwent retrospective investigation.The reasons and sites of pulmoary infection were analyzed.RESULTS The incidence of pulmonary infection of patients with RIB after radiotherapy was 68.5%.Major factors of infection were bulb palsy,invasive treatment and hormone usage.The Gram-negative bacteria were the predominant pathogens.CONCLUSIONS The nosocomial pulmonary infection is related to many factors.Antibiotic usage according to drug susceptibility results and controlling of the risk factors are important measures to reduce the rate of infection.

Objective To study the changes of learning-memory ability and synapse of hippocampus after radiation injuries. Methods Sprague-Dawley rats were grouped according to radiation dose to 20Gy group,30Gy group radiated by linear accelerator and control group were used before radiation and 120 days after radiation. Morris maze test were taken to study the learning and memory ability of rats in each group. Average escape latency and search strategy were scaled and analyzed in each group. The parameters of synapse in CA3 area of hippocampus were studied by using electron microscope and image analyzer. Results AEL of 20Gy group was (41. 17 ±10.76 ) s and score of SS was 27.13 ± 2.34 after 120 days' radiation but AEL of 30 Gy group was (78.49 ± 9.32)s and the score of SS was (23.19 ± 7.65 ) nm. There were significant statistic differences Compared with control group and before radiation (P ＜ 0.05 ). The thickness of PSD of 20 Gy group was ( 22.03 ± 6.84 ) nm after 120days' radiation and (23.19 ± 7.65 )nm in 30 Gy group. There were significant statistic differences compared with control group and before radiation. It was observed that both in 20 Gy and 30 Gy group' s the length of synaptic activity area was shorter,the curvature of synaptic interface was smaller,the width of synaptic cleft and the thickness of PSD was narrower than that of control group. Conclusion There was close relation between the changes of learning-memory ability and synapse of hippocampus after radiation injuries.

Objective To investigate the correlation between hepatitis B virus(HBV)markers and AFU,ALT,GGT.Methods 570 eases of HBV-infected people were divided into 4 groups according to the different performance modes.150 healthy people were considered as control group.rate method was used to detect AFU,ALT and GGT.Estimate the differences between the groups were compared.Results The comparison between the AFU,ALT,GGT in different performance mode of HBV and the control group showed:the differences of group 1,group 2 and group 3 were statistically significant(P＜0.01),the difference of group 4 was not significant(P＞0.01).The correlation between AFU,ALT,GGT of different performance mode showed:the changes of AFU and ALT,GGT in group 1,group 2 and group 3 were positively change-related.But the changes of AFU,ALT and GGT were not clearly related.The distribution of the rise of serum enzyme activity in different performance mode showed:the AFU,ALT and GGT rised in different proportion in group 1,group 2 and group 3.Conclusions The AFU,ALT and GGT activity of HBVinfected persons should be detected at the same time,and evaluate the damage of liver cells.This will do help to predict the progression of hepatitis.

Objective To explore the changes of nitric oxide (NO) and superoxide dismutase(SOD) in the serum and intestine mucosal and the treatment of ulcerative colitis(UC) with Tanshinone.Methods Seventy-nine patients with UC were randomly divided into the treatment group(group A,n=40) and the routine treatment group(group B,n=39).Patients in the group A were given Tanshionon and routine treatment.Patients in group B were given routine treatment.The levels of NO and SOD were measured in all patients before and after the treatment.The clinical symptom,enteroscopy,and hospital day were compared in the patients of the two groups.Twenty normal healthy controls(group C) were involved in the study.Results The NO level in the serum and intestine mucosal was significantly increased and the SOD level was significantly decreased in the patients with UC compared with those of the controls(all P