1.Odor-Dependent Hemodynamic Responses Measured with NIRS in the Main Olfactory Bulb of Anesthetized Rats.
Hyun Joo LEE ; Yunjun NAM ; Chin Su KOH ; Changkyun IM ; In Seok SEO ; Seungjin CHOI ; Hyung Cheul SHIN
Experimental Neurobiology 2011;20(4):189-196
In this study, we characterize the hemodynamic changes in the main olfactory bulb of anesthetized Sprague-Dawley (SD) rats with near-infrared spectroscopy (NIRS, ISS Imagent) during presentation of two different odorants. Odorants were presented for 10 seconds with clean air via an automatic odor stimulator. Odorants are: (i) plain air as a reference (Blank), (ii) 2-Heptanone (HEP), (iii) Isopropylbenzene (IB). Our results indicated that a plain air did not cause any change in the concentrations of oxygenated (Delta[HbO2]) and deoxygenated hemoglobin (Delta[Hbr]), but HEP and IB induced strong changes. Furthermore, these odor-specific changes had regional differences within the MOB. Our results suggest that NIRS technology might be a useful tool to identify of various odorants in a non-invasive manner using animals which has a superb olfactory system.
Animals
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Benzene Derivatives
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Hemodynamics
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Hemoglobins
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Ketones
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Odors
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Olfactory Bulb
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Oxygen
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Rats
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Spectroscopy, Near-Infrared