Objective To investigate the value of combined detection of viral capsid antibody(VCA -IgA), early antigen antibody(EA -IgG),and Rta protein antibody IgG(Rta -IgG)in the diagnosis of EB virus associated nasopharyngeal carcinoma(NPC).Methods A total of 47 nasopharynx cancer patients,45 patients with benign rhinitis and 45 healthy controls were recruited.The serum levels of VCA -IgA,EA -IgA and Rta -IgG were tested by enzyme -linked immunosorbent assay (ELISA).Specificity and sensitivity of the indicators alone and combined detection were compared using the clinical diagnosis as the gold standard.Results The expression levels of serum VCA -IgA,EA -IgA and Rta -IgG in the rhinitis group were (0.82 ±0.25),(0.74 ±0.13),(0.89 ±0.27),the levels in the NPC group were (2.16 ±0.39),(1.26 ±0.24),(3.95 ±0.76),and the levels in the healthy control group were (0.65 ±0.14),(0.51 ±0.11),(0.41 ±0.16)respectively.The levels of VCA -IgA,EA -IgA and Rta-IgG in the rhinitis group,NPC group and healthy group were significantly different (F =400.065,232.803, 740.215,P =0.000,0.000,0.000).The levels of VCA -IgA,EA -IgA and Rta -IgG in the NPC patients with different TNMstages were significantly different(F =195.679,30.878,38.561,P =0.000,0.000,0.000),and the trend of each antibody was increased with the severity of the disease.The sensitivity of VCA -IgA was the highest (85.11%),and the specificity of EA -IgA was the highest(95.56%).The sensitivity of the combined assay was 95.74%,which was higher than that of the other three combinations.Conclusion The combination of VCA -IgA, EA -IgA and Rta -IgG can reflect the expression of EBV -associated antigen to a greater extent,and it is superior to single or two combined detection in the diagnosis of EB -associated NPC,with a high clinical value.

Objective To establish an HPLC method for the determination of ginsenosides Rg1 and Re in Shenqi Granula.Methods Chromasil C18 column(250 mm?4.6 mm)was used with acetonitrile-0.05% phosphoric acid solution(21∶79)as mobile phase.The flow rate was 1 mL/min and the detected wavelength was 203 nm.Results Ginsenosides Rg1 and Re could be baseline separated with in 30 min.The average recovery rates were 99.60% and 98.5%,corresponding RSD were 1.93% and 2.31% for ginsenoside Rg1 and Re,respectively(n=5).Conclusion This method is fast and accurate and can be used for quality control of Shenqi Granula.

Objective To construct a lentiviral vector carrying rat sirt1 gene and observe the expression of sirt1 in retinal ganglion cell (RGC) of rat.Methods Rat sirt1 cDNA was inserted into pLV5 vector.After identification by sequencing analysis and PCR,the recombinant sirt1expressinglentivirus vector was packaged by cotransfecting 293T cells with packaged plasmid.Then pLV5-sirt1 was used to infect the cultured Sprague-Dawley rat RGC cell in vitro.The expressions of sirt1 protein and mRNA in infected rat RGC were detected by quantitative real-time PCR and Western blot.Results The sirt1 expression vector pLV5 was successful constructed and sequence was proved to be correct.The expression of sirt1 protein and mRNA in RGC was significantly increased than that in cells infected with control lentiviruses (P ＜ 0.05).Conclusion We have successful constructed a sirt1 expression lentivirus vector pLV5-sirt1 and it can increase the expression of sirt1 protein and mRNA in the rat retinal ganglion cells.

OBJECTIVETo establish a canine cell line with p53 gene knockdown by lentivirus- mediated RNA interference (RNAi).

METHODSFour pairs of oligonucleotide sequences of p53 gene were synthesized and cloned into the pGMLV-SC5 RNAi vector. Following confirmation by PCR and DNA sequencing, the recombinant pGMLV-p53 plasmids and packaging mix vectors were packaged into mature lentivirus to infect 293T cells. The supernatant of the infected cells was harvested to infect WRD cells, in which p53 expression was detected using Western blotting and real-time PCR. The lentivirus with the strongest p53⁻ silencing effect was packaged for infecting WRD cells at the optimal multiplicity of infection (MOI) to establish a stably infected cell line (WRD/p53⁻) screened using puromycin.

RESULTSThe lentivirus carrying p53 shRNA was constructed successfully and a virus titer of 1 × 10⁹ TU/ml. The 4 pGMLV-p53 plasmids all produced strong p53 interference affects, and pGMLV- p53A1 with the strongest effect. The stably infected cells line WRD/p53⁻ was established successfully using pGMLV-p53A1 plasmid.

CONCLUSIONThe canine cell line WRD/p53⁻ with stable lentivirus-mediated p53 silencing has been established successfully.

Animals ; Cell Line ; Dogs ; Gene Knockdown Techniques ; Genes, p53 ; Genetic Vectors ; Lentivirus ; Plasmids ; RNA Interference ; RNA, Small Interfering ; Real-Time Polymerase Chain Reaction

Objective To explore the value of acoustic radiation force impulse imaging (ARFI) technique in the evaluation of the change of kidney in rabbits with hypothermia and rewarming. Methods Sixteen New Zealand rabbits were placed in -25 ℃ environment for 8 hours, then rewarmed in 23℃ environment. Ultrasonic examinations were performed at room temperature in the time of T0and the time of T1-T8. The right kidney were evaluated by two-dimensional ultrasound and ARFI technique. Results No significant changes were found in the echo and volume of kidney from T1to T8. SWV of kidney descended greatly from T0to T1and raised from T2to T3gradually, then it descended from T3to T8gradually. Significant difference of SWV of kidney were found from T1to T8compared with that at T0(P < 0.05). Conclusions ARFI Technique can estimate the change of kidney in rabbits with hypothermia and rewarming. It can provide more useful information for clinical diagnosis.

BACKGROUND: Stroke is the leading cause of death in China, and predicting the stroke burden could provide essential information guiding the setting of medium- and long-term health policies and priorities. The study aimed to project trends associated with stroke burden in China through 2050, not only in terms of incidence and mortality but also for prevalence and disability-adjusted life years (DALYs). METHODS: Data on stroke rates in incidence, prevalence, deaths, and DALYs in China between 1990 and 2019 were obtained from a recent Global Burden of Disease study. Demographic-specific trends in rates over time were estimated using three models: the loglinear model, the Lee-Carter model, and a functional time series model. The mean absolute percentage error and the root mean squared error were used for model selection. Projections up to 2050 were estimated using the best fitting model. United Nations population data were used to project the absolute numbers through 2050. RESULTS: From 2019 to 2050, the crude rates for all measures of the stroke burden are projected to increase continuously among both men and women. We project that compared with those in 2019, the incidence, prevalence, deaths, and DALYs because of stroke in China in 2050 will increase by 55.58%, 119.16%, 72.15%, and 20.04%, respectively; the corresponding increases in number were 2.19, 34.27, 1.58, and 9.21 million. The age-standardized rate is projected to substantially decline for incidence (8.94%), death (40.37%), and DALYs (43.47%), but the age-standardized prevalence rate is predicted to increase by 10.82%. By 2050, the burden of stroke among the population aged ≥65 years will increase significantly: by 104.70% for incidence, by 218.48% for prevalence, by 100.00% for death, and by 58.93% for DALYs. CONCLUSIONS With the aging population in China increasing over the next three decades, the burden of stroke will be markedly increased. Continuous efforts are needed to improve stroke health care and secondary prevention, especially for older adults.
Male ; Humans ; Female ; Aged ; Cost of Illness ; Quality-Adjusted Life Years ; Stroke/epidemiology* ; Incidence ; Prevalence ; China/epidemiology*