Background: The most frequent congenital endocrine disorder in children is congenital hypothyroidism (CH). In Indonesia, CH’s prevalence is greater than the data currently available. However, CH newborn screening has only been carried out in a few locations across Indonesia. Therefore, there is a need to raise primary healthcare practitioners' understanding of the CH Screening Program. Materials and Methods: This study used a pre-posttest design to gauge the healthcare professionals' knowledge of newborn congenital hypothyroid screening. After intervention, a post-test was given. The categorical measures' descriptive statistical analysis results were presented as a mean, median, percentage (%), and frequency (n) distribution table. The improvement in healthcare professionals' understanding of newborn CH screening was evaluated using the Paired Samples test. The cutoff for statistical significance was p＜0.05 using SPSS version 20.0. Results: In our study, most of the participants were midwives with a total of 21/53 (39.62%) followed by nurses with 19/53 (35.83%). There was also an increase in the score where previously in the pretest only 1 person got a score above 80, then it increased to half of the participants, namely 49.06% (26/53) who got better scores. months and 37/54 (68.5%). With the Paired Sample test, we prove that there is a significant increase in the participants' scores, and the data obtained is p＜0.05, which means that there is an increase in the average data from the pretest to the post-test. Conclusion There was an improvement in pre-posttest knowledge of healthcare who had been given education about CH newborn screening.