1. Clinical value of quantitative detection of DNA aneuploidy in the diagnosis and treatment of cervical lesions in middle-aged and senile women
Lifeng WANG ; Yunheng ZHU ; Xiuxiang ZHU ; Ziyin XIA
Chinese Journal of Geriatrics 2019;38(11):1285-1288
Objective:
To investigate the clinical value of quantitative detection of DNA aneuploidy in the diagnosis and treatment of cervical lesions in middle-aged and senile women.
Methods:
A total of 1 404 middle-aged and elderly women who underwent screening for early cervical lesions were retrospectively studied.Patients were divided into the two groups: the 40-49 years old group(n=897)and the 50-78 years old group(n=507). Cervical lesions were screened by DNA ploidy analysis and the results were compared with those screened by liquid-based cytology, colposcopy and high-risk human papillomavirus(HPV).
Results:
The positive detection rate of HPV by DNA ploidy analysis was 54.4%(764/1 404). Of 1 404 patients, HPV16/18 infection accounted for 21.3%(299/1 404). The detection rate of heteroploid cells was 50.92%(715/1 404). There was a significant positive correlation between HPV infection type and cervical epithelial cell ploidy changes(
2.An analysis of ovarian tumors in elderly women
Yangfang LI ; Ling XU ; Jun XU ; Ziyin XIA ; Lifeng WANG ; Yunheng ZHU
Chinese Journal of Geriatrics 2018;37(8):898-900
Objective To investigate the pathological features of ovarian tumors in elderly women.Methods We retrospectively analyzed clinical data of 124 elderly patients with ovarian cancer who were admitted to our hospital from January 2015 to December 2017.Results Among the 124 elderly patients with ovarian tumors,there were 6 borderline tumors,19 malignant tumors,and 99 benign tumors.Postmenopausal vaginal bleeding and lower abdominal pain were the main symptoms of ovarian cancer in elderly patients.The incidence of abdominal pain in patients with benign ovarian tumors was significantly higher than in patients with malignant tumors (x2 =5.813,P <0.05);the incidence of postmenopausal vaginal bleeding in malignant tumors was significantly higher than in benign tumors(x2=8.123,P <0.05);the major pathological type of benign ovarian tumors was mucinous cystadenoma,while serous cystadenoma was the most common type of malignant tumors.Analysis of related factors showed that menopausal age was positively correlated with,and menarche age and number of pregnancies were negatively correlated with ovarian tumors;and there was no correlation with number of abortions.Conclusions Malignant ovarian tumors are common in elderly women.Clinically,attention to risk factors,early detection and diagnosis will help further reduce the malignant transformation rate and improve the quality of life for patients.
3.Effect of Aqueous Extract of Sophorae Tonkinensis Radix et Rhizoma on Bone Destruction and PI3K/Akt Signaling Pathway in Rheumatoid Arthritis
Jingbo WANG ; Jinghang YANG ; Wanyi GUO ; Panpan ZHU ; Yunheng SHEN ; Xiaohui SU ; Xiangying KONG
Chinese Journal of Experimental Traditional Medical Formulae 2022;28(23):30-37
ObjectiveTo observe the effect of aqueous extract of Sophorae Tonkinensis Radix et Rhizoma (STRR) on rheumatoid arthritis (RA) and to explore the anti-bone destruction mechanism based on phosphatidylinositol 3-kinase (PI3K)/serine/threonine-protein kinase (Akt) pathway. MethodHigh-performance liquid chromatography (HPLC) was used to determine the content of main active components in aqueous extract of STRR, and type Ⅱ collagen to induce RA (CIA) in mice. The blank group, model group, methotrexate (MTX) group (0.5 mg·kg-1), and low-dose (100 mg·kg-1) and high-dose (200 mg·kg-1) STRR aqueous extract groups were designed. Joint swelling was observed and clinical scores of CIA mice were calculated. Pathological changes of mouse joints were observed based on hematoxylin and eosin (HE) staining, and Micro-CT was performed to monitor joint destruction. TRAP staining was used to observe osteoclast formation in mouse joint, and Western blot to detect the expression of key proteins in PI3K/Akt signaling pathway in mouse joint tissue. ResultThe model group demonstrated higher degree of joint swelling, clinical scores of CIA, and degrees of synovial hyperplasia, inflammatory cell infiltration (P<0.01), and joint destruction, more osteoclasts, and higher levels of matrix metallopeptidase-9 (MMP-9), cathepsin K (CTSK), nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), PI3K, and phosphorylated-Akt (p-Akt) proteins than the blank group (P<0.01). Compared with the model group, the low-dose and high-dose aqueous extract of STRR alleviated joint swelling, reduced the clinical scores of CIA mice (P<0.05, P<0.01), relieved the pathological changes of joint tissue (P<0.01) and joint destruction, decreased osteoclasts (P<0.05, P<0.01), and lowered the levels of PI3K/Akt signaling pathway-related proteins in joint tissue of mice (P<0.01). ConclusionThe aqueous extract of STRR can significantly delay the inflammatory response of RA and especially inhibit bone destruction by down-regulating the PI3K/Akt signaling pathway.