No abstract available.
Feeding and Eating Disorders* ; Eating*

New born rat heart cells were dissociated using trypsin and/or collagenase to elucidate the dissociation efficiency of these two enzymes. And the effect of dimethyl sulfoxide during and immediately after cell dissociation was also investigated to clarify the so-called protective activity of dimethyl sulfoxide on cell performance. The results can be summarized as follows. 1. Cold trypsin 18 hours pretreatment followed by warm collagenase treatment resulted best cell viability and cell yield. 2. Single warm trypsin treatment gave the poorest result. 3. Dimethyl sulfoxide did not seem to play any protective role during or immediately after rat heart cell dissociation. It had very damaging effect on rat heart cells.
Animals ; Cell Survival ; Collagenases* ; Dimethyl Sulfoxide* ; Heart* ; Rats* ; Trypsin*

The author analysed foreign body in the airway, divided into two groups vegetable foreign body group(VFBG) and non-vegetable foreign body group(N-VFBG), according to the nature, in 88 patients aged from 5 months to 15 years who were diagnosed and treated under ventilating bronchos-copy at Department of Pediatrics and Otolaryngology, Pusan National University Hospital from 1980 to 1990. Of the 88 patients analysed, 13 were followed for review, which comprised clinical assessment, chest radiography, and perfusion lung scan. The follwing results were obtained. 1) The highest incidence was seen in the age group of 1 to 3 years old especially in VFBG, bur no difference in incidence among each age group was observed in N-VFBG, The ratio of incidence between male to female was 2:1 ein total and, in N-VFBG, the incidence of male 8 times higher than female. 2) A wide variety of foreign bodies was recovered, with peanut being by far the most common (50%) and the ratio of incidence between VFBG and N-VFBG was 6 to 4. 3) The time interval of less than 24 hours between aspiration and admission was most frequently seen in 38% and of more than 3 weeks in 11%. The time interval in VFBG and the group with left bronchial lodging was longer than of N-VFBG and the group with right bronchial lodging, each respectively but no statistical difference was observed. The time interval in the group with bronchial lodging was significantly longer than of the group with laryngeal and tracheal lodging. 4) Frequent symptoms and signs were coughing, dyspnea, fever, decreased air entry, coarse breathing sound and rales. In total, clinical manifestations were more common in VFBG, especially in the incidence of fever, decreased air entry, coarse breathing sound and rhonchi. 5) The roentgenographic findings on admission were emphysema, pneumonia and atelectasis in the order of frequency. The incidence of emphysema and complex roentgenographic findings were more common in VFBG whereas in N-VFBG radioopaque foreign body normal roentgenographic findings were more common. 6) The prevalence of bronchial lodging was over 70% regardless of age group. No difference of predilection was observed between right and left bronchial lodging before 7 years old, but after 7 years old right bronchial lodging was significantly seen. 7) The longer the time lag before admission after aspiration, the longer the duration of hospitalization was in VFBG but with no statistical significance. However, no correlation between time lag and the duration o hospitalization was observed in N-VFBG. The group with bronchial lodging had significantly longer duration of hospitalization compare to that of the group with laryngeal and tracheal lodging, The group with left bronchial lodging had longer duration of hospitalization compare to that of the group with right bronchial lodging but with no statical significance. 8) Of the 13 cases reviewed, one had abnormal lung perfusion scan in spite of normal clinical assessment and chest X-ray, who was treated on 20 days days after aspiration of peanut in the right bronchus.
Bronchi ; Busan ; Child ; Child, Preschool ; Cough ; Dyspnea ; Emphysema ; Female ; Fever ; Follow-Up Studies* ; Foreign Bodies* ; Hospitalization ; Humans ; Incidence ; Lung ; Male ; Otolaryngology ; Pediatrics ; Perfusion ; Pneumonia ; Prevalence ; Pulmonary Atelectasis ; Radiography ; Respiratory Sounds ; Thorax ; Vegetables

Myxopapillary ependymoma generally arise in the conus medullaris and filum terminale of adult spinal cord. These tumors are readily recognized due to unique histopathologic features, however, their cytologic features are not well described. When only a tiny sample is obtained, cytologic examination using crush preparation may be a useful diagnostic tool to help appropriate intraoperative diagnosis. We present the crush cytologic features of myxopapillary ependymoma arising in thoracic and lumbar spinal cord of a 13-year-old boy. The patient had complained of paraparesis and back pain for 1 month. The MRI image revealed a relatively well demarcated intramedullary mass in T11-L1 levels. Crush preparation for cytology were performed by biopsy material. Crush cytologic findings revealed high cellularity and small sized branching papillary clusters on fibrillary or mucinous background. The tumor cells had uniform round or elongated nuclei. The cytoplasmic process of tumor cells were attached to the vascular wall. Between the tumor cells and vascular walls, the perivascular collar of globoid acellular stroma with metachromatic reaction on toluidin blue stain was noted. The crush preparation of myxopapillary ependymoma is considered as a simple and highly accurate diagnostic tool for differentiation from other intramedullary neoplasms of central nervous system.
Adult ; Male ; Female ; Infant, Newborn ; Humans ; Biopsy

We wish to report 8 cages of calyceal diverticula which were diagnosed tentatively among cases of I.V.P. during the period from January 1965 to December 1971 at St. Mary's Hospital. There were 5 male and 3 female patients. The right kidney was involved in all patients The upper calyx was involved in 6 patients and the middle calyx in 2 patients. Lumbar or abdominal pain was present in 5 patients. 1 of the 8 patients had undergone appendectomy with removal of a norma1 appendix prior to diagnosis.Pyuria were found in 3 patients and one patient had single stone within the diverticulum.
Abdominal Pain ; Appendectomy ; Appendix ; Diverticulum* ; Female ; Humans ; Kidney ; Male

After developement of antimicrobial chemotherapy, morbidity and mortality from pyogenic arthritis has been reduced dramatically, but still this disease has remained as a serious and lifethreatening infectious disease of childhood or late sequelae in surviving patients. For the period of 7 years from January 1973 to December 1979, fifty nine children who were treated as septic arthritis at Severance Hospital were studied retrospectively and the results are summerized as follows. 1. Among the 59 cases, 32 cases (54.25%) were male and 27 cases (45.8%) were female. The most prevalent age was 4 to 10 years (40%). 2. Lag period to treatment in most cases was 5 days, and the most prevalent signs on admission was pain around the involved joint. 3. The most commonly affected joint was the hip joint (42.4%). The other affected sites in order of frequency were the knee, ankle and shoulder joint. 4. The underlying causes were composed of infectious focus in 18 cases, minor trauma in 6 cases, iatrogenic reason in 4 cases and unknown in 31 cases. 5. In laboratory findings, the numbers of W.B.C. and E.S.R. were increased in 66.1%, and roentgenologic studies revealed normal in 67.7%, soft tissue swelling in 21% and joint space widening in 9.7%. 6. Causative micro-organism was isolated in 41 cases: Staphylococcus aureus in 34 cases. B-hemolytic streptococcus in 3, Pseudomonas aeroginosa in 2, Enterobacter species in 2. 7. Staphylococcus aureus was highly sensitive to Cephalothin (88.2%) and Methycillin (85.3%), but was highly resistant to Penicillin (88.2%). 8, Four cases were treated non-surgically and 55 cases surgically with arthrotomy-drainage and arthrotomy-continuous irrigation. The results were satisfactory in 83% and unsatisfactory in 17%. 9. The complications were found in 14 cases: recurrence in 4, joint stiffness in 3, dislocation in 2 and sepsis in 1 case. In conclusion, a better result was obtained in cases with early diagnosis and surgical treatment with proper antibiotics.
Ankle ; Anti-Bacterial Agents ; Arthritis ; Arthritis, Infectious ; Cephalothin ; Child ; Clinical Study ; Communicable Diseases ; Dislocations ; Drug Therapy ; Early Diagnosis ; Enterobacter ; Female ; Hip Joint ; Humans ; Joints ; Knee ; Male ; Mortality ; Penicillins ; Pseudomonas ; Recurrence ; Retrospective Studies ; Sepsis ; Shoulder Joint ; Staphylococcus aureus ; Streptococcus

No abstract available.
Acidosis, Renal Tubular* ; Humans ; Infant, Newborn*

Primary hyperparathyroidism is a rare endocrine disease in children. It involves bone and joint, urinary tract, gastrointestinal tract and cardiovascular system. The main cause of these involvement is high level of PTH in serum, resulting in hypercalcemia.An 11 years old male patient who had complained of limping gait since last 18 months, showed typical laboratory and radiological findings of primary hyperparathyroidism. At the ultrasonography, computed tomography and radionuclide scanning, a well defined mass(10 X 15mm) was found on the posterior aspect of the right thyroid lobe. The mass was confirmed histologically as adenoma of parathyroid gland. The patient was successfully treated with subtotal parathyroidectomy and temporal administration of calcium and vitamin D.We report this case of primary hyperparathyroidism with brief review of the literatures.
Adenoma ; Calcium ; Cardiovascular System ; Child ; Endocrine System Diseases ; Gait ; Gastrointestinal Tract ; Humans ; Hyperparathyroidism, Primary ; Joints ; Male ; Parathyroid Glands ; Parathyroidectomy ; Thyroid Gland ; Ultrasonography ; Urinary Tract ; Vitamins

The hydrophobicity assay and DNA probe hybridization assay were compared for analysis of enterotoxigenic Escherichia coli(ETEC), heat-labile enterotoxin(LT) and heat-stable enterotoxin (ST). The ETEC isolated from diarrheal patients were serotyped and investigated for the presence of colonization factor antigens CFA/1, CFA/II, CFA/III and CFA/IV with the expression of mannose-resistant hemagglutination(MRHA) and the levels of surface hydrophobicity. The following results were obtained. 1. Out of these 48 strains, 34 strains were found to be positive for LT production by DNA probe hybridization assay. Out of 34 strains, 1 strain was ST producer, 25 strains were LT producers, and 8 strains were produced both ST+LT producers by DNA probe hybridization assay. 2. Out of 34 strains of positive DNA probe hybridization test, 31 strains was positive in the hydrophobicity test. Among strains of positive hydrophobicity test, 20, 1, and 7 strains produced only LT, only ST and both ST-LT, respectively. Screening efficiency for identifying ETEC by salting out test was 82.4% in sensitivity and 78.6% in specificity. For ETEC detection, the hydrophobicity assay was the least sensitive but was simple, rapid and a good substitute for the DNA probe hybridization assay. 4. CFAs were identified in 43.8% of ETEC strains; 2.1% of the CFAs strains with CFAs harbored CFA/I, 29.2% carried CFA/II, 16.7% carried CFA/III and CFA/IV. And 35.4% expressed none of these CFAs. CFA/I was found in ETEC of serotype 0128: K67, CFA/II was 0128: K67, 0142: K+ and 0159: K+, CFA/III was 086a: K15 and 0128: K67, CFA/IV was 0 86a: K15, 0128: K67, 0125: K70 and 0148: K+.
Colon ; DNA* ; Enterotoxigenic Escherichia coli* ; Enterotoxins ; Escherichia ; Humans ; Hydrophobic and Hydrophilic Interactions* ; Mass Screening ; Sensitivity and Specificity

No abstract available.
Adrenal Cortex Hormones* ; Glaucoma*