No abstract available.
Animals ; Embryonic Development* ; Embryonic Structures* ; Female ; Mice* ; Pregnancy

Ehlers-Danlos Syndrome(EDS) is an inherited disorder of the connective tissue disease that is usually transmitted as autosomal dominant trait. The defect in the biogenesis of collagen results in varying degree of skin hyperextensibility, joint hypermobility, skin fragility and bruising. There have been several reports of cardiac or great vessel abnormalities in patients with the EDS. A 45-year-old Woman was admitted to Maryknoll hospital because of orthopnea, palpitation and epigastric discomfort. Physical examination reveals multiple variable sized bruises and increased hyperelasticity on skin, and hypermobile joint in knee and proximal interphalanges of both hands. Mitral valvular prolapse and atrial septal defect were detected by echocardiography. We report a case of EDS accompanied by mitral valvular prolapse and ostium secundum atral septal defect with brief review of literature.
Organelle Biogenesis ; Collagen ; Connective Tissue Diseases ; Contusions ; Echocardiography ; Ehlers-Danlos Syndrome* ; Female ; Hand ; Heart Septal Defects, Atrial* ; Humans ; Joint Instability ; Joints ; Knee ; Middle Aged ; Physical Examination ; Prolapse* ; Skin

PURPOSE: The purpose of this study is to compare birth outcomes based on maternal ethnicity in Korea. METHODS: Using the birth data of Statistics Korea from 2010 to 2015, this study selected data from infants with a Vietnamese mother and Korean father (Vietnamese/Korean group), and a Chinese mother and Korean father (Chinese/Korean group), to compare them with that of a Korean mother and Korean father (Korean/Korean group). The newborn infants' birth outcomes and parental characteristics were investigated, and trends in annual changes were compared. In addition, this study investigated whether the mother's ethnicity affected the mean birth weight. RESULTS: Gestational age and birth weight were highest in the Chinese/Korean group, and were slightly lower in the Korean/Korean and Vietnamese/Korean group, in that order. The highest rate of preterm birth before 37 weeks and low birth weight rate were observed in the Vietnamese/Korean group; 4.62% and 4.26%, respectively. From 2010 to 2015, the mean gestational age decreased in all the three groups, and the preterm birth rate increased at gestational ages less than 37 weeks. However, the birth weight decreased only in the Korean/Korean group, but increased in the Chinese/Korean and Vietnamese/Korean groups. In addition, we found that factors such as parents' educational levels, percentage of hospital births, and appropriate maternal age improved in the Vietnamese/Korean group. CONCLUSION: This study confirms that the mean birth weight and low birth weight rate are affected by maternal ethnicity in Korea. Therefore, careful research and active national support policies are needed.
Asian Continental Ancestry Group* ; Birth Weight ; Continental Population Groups ; Cultural Diversity ; Emigrants and Immigrants ; Fathers ; Gestational Age ; Humans ; Infant ; Infant, Low Birth Weight ; Infant, Newborn ; Korea* ; Maternal Age ; Mothers ; Parents ; Parturition* ; Premature Birth

STATEMENT OF PROBLEM: Reducing treatment time in implant dentistry is a matter of main concern. There are so many factors affecting the success rate of immediate or early loaded implant for the initial bone response. The especially microscopic properties of implant surfaces play a major role in the osseous healing of dental implant. PURPOSE: The aims of this study were to perform a histologic and histomorphometric comparison of the healing characteristics anodically roughened surface, HA coated surface and RBM surface implant, and to compare of ISQ values measured by Osstell(TM) for resonance frequency analysis in dogs mandible during 2 weeks. MATERIAL AND METHOD: Bone blocks from 2 dogs were caught after covered healing for 0 day(2h); Group I, 1 week; Group II and 2 weeks; Group III. One longitudinal section was obtained for each implant and stained with hematoxylin and eosin. Histomorphometric analysis was done with Kappa Imagebase system to calculate bone-to-implant contact and bone volumes inside the threads. ISQ values were measured in every time of surgery schedule. CONCLUSION: The experiment revealed that : 1. The percentages of bone-to-implant contact on the fixture in each group were not significantly different(P > 0.05). 2. The percentages of bone area inside the threads on the fixture in each group were not significantly different(P > 0.05). 3. The ISQ level showed clinical stability of each fixture during 2 weeks(all ISQ level >_71).
Animals ; Appointments and Schedules ; Dental Implants ; Dentistry ; Dogs* ; Eosine Yellowish-(YS) ; Hematoxylin ; Mandible

STATEMENT OF PROBLEM: One of the common problems of dental implant prosthesis is the loosening of the screw that connects each component, and this problem is more common in single implant-supported prostheses with external connection. PURPOSE: The purpose of this study was to examine the changes of detorque values of abutment screws with external connection in different abutment heights. MATERIALS AND METHODS: After cyclic loading on three different abutment heights, detorque values were measured. Abutments were retained with titanium abutment screws tightened to 30 Ncm (30.5 kgmm) with digital torque gauge as recommended by the manufacturer. Replacing abutments, implants and titanium abutment screws with new ones at every measurement, initial detorque values were measured six times. In measuring detorque values after cyclic loading, Avana Cemented Abutments of 4.0 mm collar, 7.0 mm height (Osstem Co., Ltd., Seoul, Korea) were used with three different lengths of 5.0, 8.0, 11.0 mm. Shorter abutments were made by milling of 11.0 mm abutment to have the same force-exercised area of 4.5 mm diameter. Sine curve force (20N-320N, 14Hz) was applied, and detorque values were measured after cyclic loading of 2 million times by loading machine. Detorque values of initial and after-loading were measured by digital torque gauge. One-way ANOVA was employed to see if there was any influence from different abutment heights. RESULTS: The results were as follows : 1. The initial detorque value was 27.8+/-0.93 kgmm, and the ratio of the initial detorque value to the tightening torque was 0.91(27.8/30.5). 2. Measured detorque values after cyclic loading were declined as the height of the abutment increased, that was, 5.0 mm; 22.3+/-0.82 kgmm, 8.0 mm; 21.8+/-0.93 kgmm, and 11.0 mm; 21.3+/-0.94 kgmm. 3. One-way ANOVA showed no statistically significant differences among these (p> 0.05). 4. Noticeable mobility at the implant-abutment interface was not observed in any case after cyclic loading at all.
Dental Implants ; Prostheses and Implants* ; Seoul ; Titanium ; Torque

STATEMENT OF PROBLEM: The long-term success of implants is the development of a stable direct connection between bone and implant surface, which must be structural and functional. To improve a direct implant fixation to the bone, various strategies have been developed focusing on the surface of materials. Among them, altering the surface properties can modify cellular responses such as cell adhesion, cell motility and bone deposition. PURPOSE: This study was to evaluate the cellular behaviors on the surface-modified titanium by morphological observation, cellular proliferation and differentiation. MATERIAL AND METHODS: Specimens were divided into five groups, depending on their surface treatment: electropolishing(EP) anodizing(AN), machining(MA), blasting with hydroxyapatite particle(RBM) and electrical discharge machining(EDM). Physicochemical properties and microstructures of the specimens were examined and the responses of osteoblast-like cells were investigated. The microtopography of specimens was observed by scanning electron microscopy(SEM). Surface roughness was measured by a three-dimensional roughness measuring system. The microstructure was analyzed by X-ray diffractometer(XRD) and scanning auger electron microscopy(AES). To evaluate cellular responses to modified titanium surfaces, osteoblasts isolated from neonatal rat were cultured. The cellular morphology and total protein amounts of osteoblast-like cell were taken as the marker for cellular proliferation, while the expression of alkaline phosphatase was used as the early differentiation marker for osteoblast. In addition, the type I collagen production was determined to be a reliable indicator of bone matrix synthesis. RESULTS: 1. Each prepared specimen showed specific microtopography at SEM examination. The RBM group had a rough and irregular pattern with reticulated appearance. The EDM-treated surface had evident cracks and was heterogeneous consisting of broad sheet or plate with smooth edges and clusters of small grains, deep pores or craters. 2. Surface roughness values were, from the lowest to the highest, electropolished group, anodized group, machined group, RBM group and EDM group. 3. All groups showed amorphous structures. Especially anodized group was found to have increased surface oxide thickness and EDM group had titaniumcarbide(TiC) structure. 4. Cells on electropolished, anodized and machined surfaces developed flattened cell shape and cells on RBM appeared spherical and EDM showed both. After 14 days, the cells cultured from all groups were formed to be confluent and exhibited multilayer proliferation, often overlapped or stratified. 5. Total protein amounts were formed to be quite similar among all the group at 48 hours. At 14 days, the electropolished group and the anodized group induced more total protein amount than the RBM group(P<.05). 6. There was no significant difference among five groups for alkaline phosphatase(ALP) activity at 48 hours. The AN group showed significantly higher ALP activity than any other groups at 14 days(P<.05). 7. All the groups showed similar collagen synthesis except the EDM group. The amount of collagen on the electropolished and anodized surfaces were higher than that on the EDM surface(P<.05).
Alkaline Phosphatase ; Animals ; Bone Matrix ; Cell Adhesion ; Cell Movement ; Cell Proliferation ; Cell Shape ; Edible Grain ; Collagen ; Collagen Type I ; Durapatite ; Osteoblasts* ; Rats ; Surface Properties ; Titanium*

PURPOSE: To evaluate the CT and US features of the colonic villous tumors. MATERIALS AND METHODS: We retrospectively reviewed the CT findings of 11 cases with histologically proved colonic villous tumor. CT parameters evaluated were morphological appearances and enhancing pattern (size, shape, margin, presence or absence of fronds, bowel wall thickening). CT features of six cases with malignant change were compared with five tumors without malignant change. US features available in 10 patients were also analyzed. RESULTS: On CT, thetumors showed irregular margin(n=9), presence of fronds(n=6), lobulated shape(n=11), with pericolonic invasion(n=1). Six cases with malignant change were larger(mean, 6.8cm in diameter) than those without malignant change(mean, 3.3 cm). US features in 10 cases were intraluminal mass(n=5), colonic wall thickening(n=5), with variable echogenicity. CONCLUSIONS: Colonic villous tumor appeared as a nonspecific mass on CT and US with adifficulty in distinguishing from colon carcinoma.
Colon* ; Humans

BACKGROUND AND OBJECTIVES: Many diabetic patients suffer from cardiomyopathy, even in the absence of vascular disease. The aim of this study was to see if dietary antioxidant supplementation has an inhibitory effect on the progression of cardiac tissue damage in streptozotocin (STZ)-induced diabetic rats. MATERIALS AND METHODS: Sprague-Dawley male rats (n=60) were used as experimental animals; they were divided into the normal control group and the diabetic group. Eight weeks after STZ injection (65 mg/kg of body weight), the products of lipid peroxidation (malondialdehydes, MDA), and the antioxidant enzyme superoxide dismutase (SOD), and catalase activities were determined in the cardiac tissue homogenates. The cardiac tissues were studied by light microscopy (LM) and electron microscopy (EM), and the tissue lesions were graded by a semiquantitative score. RESULTS: The histologic scores for perivascular fibrosis, interstitial fibrosis and myocardial necrosis according to LM were significantly lower in the combined vitamin C & E treated rats than in the diabetic control rats. The ultrastructural scores for the overall cardiac morphology, mitochondria and myofilaments, according to EM, were significantly lower in the vitamin E treated rats and the combined vitamin C & E treated rats than in the diabetic control rats, even though this was of less magnitude than that in the insulin-treated diabetic rats. CONCLUSION: These results suggest that antioxidants such as vitamin C & E might have a beneficial effect on diabetes as an adjunct therapy against lipid peroxidation and diabetic cardiomyopathy, in addition to the effects of instituting strict measures for controlling the blood glucose.
Animals ; Antioxidants* ; Ascorbic Acid ; Blood Glucose ; Cardiomyopathies ; Catalase ; Diabetes Mellitus, Experimental ; Diabetic Cardiomyopathies ; Fibrosis ; Humans ; Lipid Peroxidation ; Male ; Microscopy ; Microscopy, Electron ; Mitochondria ; Myofibrils ; Necrosis ; Rats* ; Rats, Sprague-Dawley ; Streptozocin ; Superoxide Dismutase ; Vascular Diseases ; Vitamin E ; Vitamins

BACKGROUND: ATP is an endogenous nucleotide that has potential electrophysiologic effects. The effects of ATP are largely mediated by its metabolite, adenosine, which acts on A1 receptor in myocardium and mediates negative chronotropic, dromotropic, and inotropic action. The purpose of this study is to determine the efficacy and side effects of ATP in the treatment of paroxymal supraventricular tachycardia(PSVT). METHODS: We analyzed 28 episodes of PSVT in 22 patients(7 men and 15 women, mean age 467.+/-15.9 years), who were selected from Jan. 1992 to Sep. 1992. After measuring blood pressure and obtaining a standard 12 leads electrocardiogram(ECG), we rapidly injected ATP intravenously with dose of 10, 15, 20mg every 1 min, until the termination of PSVT. We recorded peri-injection and post-injection ECG, interval between ATP injection and the termination of PSVT, the pause after termination, side effects and blood pressure immediately after termination. RESULTS: 1) Among 28 episodes of PSVE, 26 episodes(92.9%) were terminated successfully : 21 episodes(75%) with 10mg, 3 episodes(10.7%) with 15mg, and 2 episodes(7.2%) with 20mg of ATP. 2 episodes(7.1%) were failed. 2) The PSVT ended 21.2+/-9.2 seconds after injection of ATP(maximally 40 seconds). 3) The mechanisms of PSVT in 9 patients were atrioventricular nodal reentrant tachycardia(AVNRT), those in 13 patients were atrioventricular reciprocating tachycardia(AVRT). 4) The side effects of ATP included 13 episodes of chest pain(46.6%), 9 episodes of ventricular arrhythmia(32.1%), 6 episodes of sinus pause(21.4%) and 5 episodes of atrioventricular block(17.9%), and these were uncomplicated and short-lasting. CONCLUSION: The ARP is a safe and effective therapy for the conversion of PSVT to sinus rhythm and perhaps more importantly, a valuable new approach to the management and diagnosis of wide-complex tachycardia.
Adenosine Triphosphate ; Adenosine* ; Blood Pressure ; Diagnosis ; Electrocardiography* ; Female ; Humans ; Male ; Myocardium ; Tachycardia ; Tachycardia, Supraventricular* ; Thorax

STATEMENT OF PROBLEM: Platelet-rich plasma(PRP) is well known to be very effective method to stimulate and accelerate the healing of bone and soft tissue. However, there are few reports which deal with the mechanisms of the PRP on the activation of the osteoblasts. PURPOSE: This study was aimed to investigate the effect of growth factors in PRP on the activity of osteoblasts. MATERIAL AND METHOD: To evaluate the effect on human, human osteoblast cell line was cultured. PRP was extracted from the blood of a healthy volunteer. Using the recombinant growth factors of PDGF, TGF-beta, IGF-1, bFGF which are mainly found at bone matrix and their neutralizing antibody, the effect of PRP on the attachment and proliferation of osteoblasts was evaluated. To evaluate the autocrine and paracrine effects, conditioned media(CM) of PRP was made and compared with PRP. By the western blot analysis, the expression of growth factors in PRP, CM was examined. Cell morphology was compared by the light microscope. RESULTS: 1) The effects of CM on osteoblast were similar to the effects of PRP. 2) PRP, CM, recombinant TGF-beta, bFGF, IGF-1 showed significantly higher cellular attachment than control(P<0.05) in the cell attachment assay. In the cell proliferation assay, PRP, CM, recombinant TGF-beta, IGF-1, bFGF, PDGF increased significantly cell proliferation(P<0.01). Among the recombinant growth factors, IGF-1 showed the highest cellular attachment and proliferation. 3) In the western blot assay, bFGF, IGF-1, PDGF were equally expressed in PRP and CM. 4) The attachment of osteoblast cell decreased significantly after the addition of neutralizing antibody against TGF-beta, IGF-1(P<0.05). In the cell proliferation assay, the addition of neutralizing antibody against TGF-beta, bFGF, PDGF, IGF-1 decreased significantly the cellular proliferation(P<0.05). The amount of decreasing in the cell attachment and proliferation is the highest in ab-IGF-1. 5) The cells in control group were flattened and elongated with a few cellular processes in the a light microscope. But, the cells appeared as spherical, plump cells with well developed cellular processes in experimental groups. The cells in PRP and CM had more prominent developed features than recombinant growth factor groups. CONCLUSIONS: These findings imply that PRP maximize the cellular activity in early healing period using the synergistic effect, autocrine, paracrine effects of growth factors and increase the rate and degree of bone formation.
Antibodies, Neutralizing ; Blotting, Western ; Bone Matrix ; Cell Line* ; Cell Proliferation ; Culture Media, Conditioned ; Healthy Volunteers ; Humans ; Insulin-Like Growth Factor I ; Intercellular Signaling Peptides and Proteins* ; Osteoblasts* ; Osteogenesis ; Platelet-Rich Plasma* ; Transforming Growth Factor beta