Objective To investigate the relationship between some autoantibodies including anti-? 2-glycoprotein Ⅰ (anti-? 2GPⅠ) and chronic liver disease. Methods A cohort of patients with chronic hepatitis B (CHB) and post-hepatitis B cirrhosis (PHBC) was studied for the serum anti-? 2GPⅠ levels by ELISA with purified ? 2GPⅠ as antigen. The serum dsDNA, smooth muscle antibody (SMA) and ribonucleoprotein antibody (RNPA) were also detected. Results High positive rate was observed in patients with CHB or PHBC (20.9%, 9/43; 49.3%,35/75) comparing with that in control group (3.1%, 1/32) ( P

Objective To study the relationship between anti-? 2-glycoprotein I(a? 2GPⅠ) and extracellular matrix (ECM) in patients with post-hepatitis B cirrhosis (PHBC).Method The of patients with chronic hepatitis B (CHB) and PHBC were studied for the serum a? 2GPⅠ levels by ELISA with purified ? 2GPⅠ as antigen, and we investigated serum level of collagen IV (IV-C)?hyaluronic acid (HA) and laminin (LN) in these patients.Result High positive rate was observed compared with control group (P

BACKGROUND:Hepatoblasts are one of high-grade cell sources for severe liver disease treatment.Efficient separation and purification of hepatoblasts from fetal liver has important practical significances.OBJECTIVE:To introduce the preparation of hepatoblasts from fetal liver,conduct a transversal comparison of these preparation methods,and point out their advantages and shortcomings,so as to provide the evidences for the experimental and clinical choices of hepatoblasts.RETRIEVAL STRATEGY:Using the keywords of "fetal liver progenitor/hepatoblasts,isolation/purification",we searched the articles about fetal liver hepatoblasts separation and purification in PUBMED between January 1997 and August 2007 in English.At the same time,we searched the relative articles in CNKI using the keywords of "liver,embryo,endothelial progenitor/hepatoblasts" between January 1997 and August 2007 in Chinese.In addition,some related books and foreign articles were checked in the laboratory of Jilin University.After the first examination,the articles published in the authority magazines within five years were given prior consideration.Exclusive criteria were those repetitive researches or Meta-analysis.Totally 245 articles were collected,we selected 33 representative articles about hepatoblasts separation and purification methods.LITERATURE EVALUATION:Among 31 included articles,10 ones introduced the study background,5 ones studied the fetal liver cell dissociation,9 ones referred to the hepatoblasts separation and purification,and 7 ones indicated the problems and prospect in this field.DATA SYNTHESIS:The hepatic epithelial cell precursor cells(also known as hepatoblasts) with hepatic and biliary epithelial cell differentiation potential isolated from fetal liver,could in vitro proliferate and differentiate,after transplantation in vivo they show a good homing,and the ability to integrate and regenerate.Hepatoblasts isolation technology is being improved daily,and the related researches on this field will boost cell-based liver therapy in clinical application.CONCLUSION:Improvements and combination of the various isolation methods,as well as new and high-specific antigen discovery will further improve the isolation efficiency of hepatoblasts from fetal liver.

Objective To investigate the expression of a novel imprinted gene,PEG10,in human gastric adenocarcinoma,and the effect of PEG10 on cell growth and proliferation of gastric cancer cells.Methods The PEG10 mRNA expressions in 40 human gastric adenocarcinoma,the corresponding adjacent normal tissues and 6 nomal gastric tissues were detected by RT-PCR.The expression vectors of PEG10 were constructed and transfected into gastric cancer cell line MKN45 which had no endogenetic PEG10 expression.Cell growth ability was measured by MTT assay.Results High PEG10 mRNA expression level was detected in 9 of 20(45.0%) human gastric adenocarcinoma which was significantly higher than those of the matched normal tissues(10.0%)(P

Objective To investigate the relationships between low one-minute Apgar score and the prognosis of extremely preterm infants (EPI) and extremely low birth weight infants (ELBWI). Methods Altogether 50 EPI and ELBWI who had a low one-minute Apgar score ( ≤ 7) and were admitted to the Neonatal Intensive Care Unit (NICU) of Peking University Third Hospital from January 1,2010 to December 31, 2015 were enrolled in this study. All of them were divided into two groups according to their Apgar score: mild group (4-7) and severe group (0-3). Medical records of the subjects were reviewed and an at least 18 months follow up study was conducted. Conditions of all subjects during perinatal period and hospitalization were summarized. Outcomes and follow-up results were compared between the two groups by using Fisher exact test. Results (1) General information: Fifty infants were involved, among which 37 had a mild low Apgar score and 13 had a severe low Apgar score. The mean gestational age was (27.7±2.1) weeks and the mean birth weight was (884.4±174.3) grams. (2) Main complications (some infants with more than one complication): There were 42 cases of neonatal respiratory distress syndrome, 12 cases of pulmonary hemorrhage, 21 cases of bronchopulmonary dysplasia, 31 cases of patent ductus arteriosus, 36 cases of intraventricular hemorrhage, 22 cases of white matter damage and six cases of retinopathy of prematurity. (3) Outcomes: The survival rate was 48% (24/50) and the mortality rate was 52% (26/50). Among the 26 infants, five died despite treatment and 21 died within 72 hours after their parents giving up treatment. There were no significant differences in the survival rates, mortality rates and rates of abandon treatment between the two groups [43% (16/37) vs 8/13; 11%(4/37) vs 1/13; 46% (17/37) vs 4/13; Fisher exact test, all P>0.05]. (4) Follow-up results: Twenty-one infants were followed-up to at least 18 months of age, among which four were normal, 10 had growth retardation and recurrent respiratory tract infection and seven had motor development retardation. The incidence of motor development retardation in severe group was higher than that in mild group, and the difference between them was statistically significant (5/8 vs 2/13, Fisher exact test, P=0.046). Conclusions EPI or ELBWI with a low one-minute Apgar score have many nosocomial complications, resulting in high mortality and high incidence of motor development retardation.

Objective To explore the influence of regular measurement of transcutaneous bilirubin (TcB) on clinical outcome of neonatal hyperbilirubinemia.Methods Healthy term and late-preterm newborns (gestational age ≥ 35 weeks) born between January 1 and December 31,2013 at Peking University Third Hospital were reviewed retrospectively.All neonates were divided into monitoring group (n=1 071)or control group (n=2 437) according to whether regular monitoring of TcB was performed.General clinical information and TcB levels on admission were recorded.The incidence of hyperbilirubinemia and readmission for hyperbilirubinemia,level of bilirubin and length of hospital stay were compared between the two groups.Chi-square test or independent sample t test were used for statistical analysis.Results (1) The incidence of hyperbilirubinemia and readmission rate for hyperbilirubinemia were lower in monitoring group when compared with control group [4.7％ (50/1 071) vs 7.1％ (172/2 437) and 1.3％ (14/1 071) vs 3.3％ (81/2 437) respectively,P＜0.05].There was no significant difference of bilirubin level and hospital stay between the two groups (P＞0.05).(2) Of all the 222 hyperbilirubinemia patients,the number of neonates with gestational age ＜37 weeks,37-38 weeks and ＞38 weeks was 26 (11.7％),33(14.9％) and 163(73.4％),respectively.There was no significant difference of gestational age,birth weight and gender proportion between the two groups.(3) The follow-up rate in January was 41.5％ (49/118),and the mean follow-up rate in February,April,July and October (with revised follow-up methods) was 83.1％ (345/415),higher than that in January (x2=82.505,P＜0.01).(4) The first three causes of hyperbilirubinemia in all 222 patients were early-onset breast milk jaundice (72 cases,32.4％),perinatal causes (60 cases,27.0％) and unknown reasons (50 cases,22.5％).The first three causes of hyperbilirubinemia in monitoring group (50 cases) were unknown reasons (24 cases,48.0％),early-onset breast milk jaundice (14 cases,28.0％) and hemolysis (9 cases,18.0％).Those in the control group (172 cases) were early-onset breast milk jaundice (58 cases,33.7％),perinatal causes (57 cases,33.1％) and unknown reasons (26 cases,15.1％).Conclusions Regular monitoring of TcB can reduce the incidence ofhyperbilirubinemia and the readmission for hyperbilirubinemia.

Gynecological malignant tumors bring great pain and psychological pressure to patients and affect the quality of life of patients seriously. Their families are faced with serious mental burden during the caring of patients especially spouses. Their quality of life is also affected seriously. This article reviewed the research progress in the quality of life of caregivers including the impact of general quality of life of caregivers for patients with gynecological malignancies, the impact on sexual life between couples and the strategies to improve the quality of life of caregivers. It came to the conclusion that only scientific and effective nursing interventions could improve the quality of life of caregivers.

Objective To systematically review the effects of Yoga on cancer related fatigue in breast cancer patients. Methods While foreign literatures searched from PubMed, EMBASE, CINAHL, ISI Web of Knowledge, EBSCO, MEDLINE, etc, Chinese literatures came from CNKI, CBM, VIP and Wanfang Data, besides Google scholar was assisted to collect all randomized control trails ( RCTs) about Yoga releasing breast cancer related fatigue. Retrieval started from creating database to1 July 2014 without language restriction. Cochrance evaluation manual was used to evaluate the RCTs, and RevMan 5. 1 software was used for Meta analysis. Results A total of 9 RCTs met the inclusion criteria, with a total of 623 breast cancer patients, including 342 patients in the yoga group ( intervention group) and 281 patients in the usual care group ( control group) . The Meta-analysis results revealed that Yoga played certain effects to release breast cancer related fatigue. Conclusions Whether Yoga, as a effective method to release breast cancer related fatigue, can be generally promoted in clinical required clinical high quality, large sampling and multicenter RCTs to support.

The article introduces the concept of stigma and the measurement tools of stigma in abortion women as well as research progress of sociological factors, quality of life and interventions of abortion women. This article points out that china is still lack of related research. Strengthening the care for artificial abortion women from people of all walks of life is called on.

OBJECTIVESTo study the interaction of beta-2-glycoprotein I (beta 2GPI) with the membrane of hepatocytes and determine whether beta 2GPI participates in HBV infection.

METHODSLigand blotting, fluorescence microscopy, and fluorescence activated cell sorter (FACS) analysis were used to detect the specific interaction of beta 2GPI with the hepatoma cell line smmc7721, the gastric carcinoma cell line SGC7901, and the lymphoma cell line HL-60.

RESULTSA specific 40 kDa beta 2GPI band was observed by ligand blotting in the case of smmc7721 cells. No such band was observed in SGC7901 or HL-60 cells. Fluorescence microscopy also revealed specific binding of FITC-beta 2GPI to smmc7721 cells, but neither to SGC7901 nor HL-60 cells. FACS analysis demonstrated that the binding rate of FITC-beta 2GPI to smmc7721 cells was significantly higher than these in SGC7901 and HL-60 cells (P < 0.01). The binding rate to smmc7721 cells did not increase with increasing amounts of FITC-beta 2GPI.

CONCLUSIONSThere is a specific beta 2GPI-binding protein on the membrane of hepatoma cells in cell line smmc7721 which as the beta 2GPI receptor may participate in HBV infection of hepatocytes.

Animals ; Carcinoma, Hepatocellular ; Cell Membrane ; metabolism ; Flow Cytometry ; Glycoproteins ; metabolism ; Hepatocytes ; metabolism ; Liver Neoplasms, Experimental ; metabolism ; Platelet Glycoprotein GPIb-IX Complex ; analysis ; Platelet Membrane Glycoproteins ; Tumor Cells, Cultured ; beta 2-Glycoprotein I