1.Diagnosis and management of nasal and pharyngeal dendritic cell tumor-case report and literature review.
L V DAN ; Yunfeng HUANG ; Hui YANG ; Shixi LIU ; Haiyang WANG ; Min WANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(8):522-526
OBJECTIVE:
To investigate the clinical manifestation, pathological characteristics, treatment and prognosis of dendritic cell tumor.
METHOD:
Four cases of nasal and pharyngeal dendritic cell tumor were described, including two cases of follicular dendritic cell sarcoma (FDCS), one case of Langerhans cell histiocytosis (LCH) and one case of Langer hans cell sarcoma (LCS). One of the patients with FDCS received multimodality therapy (surgery combined with chemotherapy), and the other patient only received chemotherapy and radiotherapy. The patients with LCH or LSC were treated by surgery.
RESULT:
Of the two FDCS patients, one achieved complete remission after treatment by surgery combined with four cycles of CHOP chemotherapy regimen and concurrent radiotherapy (50 Gy), and the other who only received chemotherapy and radiotherapy survived with tumor for more than seven months of follow up. The patient of LCH was followed up for more than 2 years after surgery without recurrence or metastasis. The patient of LCS did not undergo radiotherapy or chemotherapy after surgery and died after 10 months of follow up.
CONCLUSION
Dendritic cell tumor is a group of very rare tumor and can be easily misdiagnosed in clinic, the confirmed diagnosis of which relies on histopathological features, immunohistochemistry combined with electron microscopy. FDCS, LCH and LCS have different pathological features, immunophenotypes and prognosis.
Child
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Child, Preschool
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Dendritic Cell Sarcoma, Follicular
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pathology
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therapy
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Female
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Follow-Up Studies
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Humans
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Male
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Middle Aged
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Nasopharyngeal Neoplasms
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pathology
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therapy
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Prognosis
2.Ideas and Briefing about Regulatory Requirements for Laboratory Developed Tests in the US.
Zhaozhao HAN ; Yang ZHAO ; Yunfeng L ; Jinchun DONG ; Gang DENG
Chinese Journal of Medical Instrumentation 2022;46(5):534-537
As a special kind of in vitro diagnostic devices(IVDs), laboratory developed tests(LDTs) are of great significance to the development of clinical laboratories. This study aims to explore the regulatory requirements ideas of LDTs. By introducing the development of LDTs and the changing of regulatory requirements in the United States, combing the current regulatory framework and discussing relevant ideas in the regulatory requirements of LDTs.
Clinical Laboratory Services
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Laboratories
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Reagent Kits, Diagnostic
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United States
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United States Food and Drug Administration
3.Expression and clinical significance of DNAJB11 in epithelial ovarian cancer.
Yao GUORONG ; F U YUNFENG ; L I YANLI ; Zhou CAIYUN ; L V WEIGUO
Journal of Zhejiang University. Medical sciences 2017;46(2):173-178
To study the expression of DNAJB11 protein in epithelial ovarian cancer tissues and its clinical significance.Immunohistochemistry was used to examine DNAJB11 expressions in 105 tissue specimens of ovarian epithelial carcinoma, 23 normal ovarian tissues, 17 tissues of benign tumor, and 13 tissues of of borderline tumor. The correlations between protein expression and clinicopathological factors were analyzed by Chi square test.The correlations between protein expression and survival were analyzed by Kaplan-Meier and Cox regression.Positive expression of DNAJB11 protein was observed in 0.0% in normal ovary and benign tumor, 7.69% in borderline tumor, and 78.10% in epithelial ovarian cancer, respectively. Positive expression of DNAJB11 protein was significantly higher than the rest of the ovarian tissues and normal ovarian tissues (<0.001).Higher expression of DNAJB11 was more prevalent in tissues from patients with advanced FIGO stages, high serum CA125, poor histological differentiation, and serous cancer. Kaplan-Meier curves revealed that higher expression of DNAJB11 was significantly associated with poor disease-free survival and overall survival.Multivariate survival analysis revealed that strong positive expression of DNAJB11 was an independent prognostic factor for disease-free survival and overall survival.DNAJB11 may play a role in tumorigenesis and progression of epithelial ovarian cancer.Strong positive expression of DNAJB11 was an independent prognostic factor in epithelial ovarian cancer.