AIM: To study the antipyretic, analgesic and antiinflammatory actions of dexketoprofen.　METHODS: Hot-plate and tail-flick models in mice, fever model in rats and rabbits induced by carrageenin or typhoid vaccine respectively, ear swelling induced by dimethylbenzene in mice; foot pad swellings induced by carrageenin and granuloma induced by cotton in rats were adopted.　RESULTS: Dexketoprofen (7.5,15,30 mg*kg-1) could significantly prolong latency of analgesic in hot-plate and tail-flick models in mice and reduce carrageenin-induced fever in rats, and inhibit dimethylbenzene-induced ear swelling in mice. Dexketoprofen (1.9,3.8,7.5 mg*kg-1) could significantly inhibit typhoid vaccine-induced fever in rabbits; Dexketoprofen (3.8,7.5,15 mgkg-1) could also significantly inhibit the swelling of foot pad induced by carrageenin, and the granuloma induced by cotton in rats.　CONCLUSION: Dexketoprofen has antipyretic, analgetic and antiinflammatory effect.

AIM To establish the model of type Ⅱ collagen(CⅡ) induced arthritis in mice. METHODS The type Ⅱ collagen induced arthritits(CIA) mice were induced by intradermal injection with CⅡ and complete Freund adjnvant emulsion. RESULITS In comparison with control mice, the arthritic swelling and index were significant increased. The responses of CⅡ induced delayed type hypersensitivity were remarkably positive, and IgG anti bodies to CⅡ in serum could be detected in CIA mice. CONCLUSION The model CIA mice was successfully established.

AIM To study the antiinflammatory and immunomodulatory effects of actarit(Acta). METHODS To abserve the change of primary and secondary inflammation,immune function on adjuvant arthritis(AA) rats treated with Acta and to detect the effect of Acta on T cell subgroup in mice in vitro. RESULTS Acta(10,30,90 mg?kg -1 ) intragastric injection(ig) did not significantly inhibit primary reaction of AA in rats. But Acta(10,30,90 mg?kg -1 ?11 d) ig signifcantly inhibited secondary reaction (secondary inflammatory swelling、 multiple arthritis) of AA rats. The lowered response of ConA induced splenolytes and the decreased IL 2 synthesis were restored to normal in AA rats treated with Acta, while the elevated IL 1 released from peritoneal macrophate (PM?) was reduced. Acta(1～100 ?mol?L -1 ) could inhibit ConA induced T h cells and enhance ConA induced T s cells culture in vitro . CONCLUSION Acta do not show obvious antiinflammatory action. But Acta has therapeutic action on AA rats, which may be related to its immunomodulatory activities,mainly through modulate cell mediated immunity.

AIM To study the therapeutic effects of Deketoprofen trometamol (D KPT) on adjuvant arthritis (AA) and its side effects on gastroduodena. METHEDS The change of primary and secondary inflammatory and the injury effect of gastroduodena treated with D KPT were observed. The effect of D KPT on prostaglandin E 2 (PGE 2) produced by peritoneal macrophage (PM) on AA rats in vitro was also observed. RESULTS D KPT (2 5, 5, 10 mg?kg -1 ) significantly inhibited primary inflammatory, secondary inflammatory and multiple arthritis of AA rats. D KPT(10 -8 ,10 -7 ,10 -6 ,10 -5 ,10 -4 mol?L -1 )inhibited the elevated PGE 2 released from PM of AA rats in vitro . D KPT (10 mg?kg -1 ) significantly injured gastroduodena on AA rats, but the degree of injuries was less than that of ketoprofen (KP) (10 mg?kg -1 ). CONCLUSION D KPT has therapeutic effects on AA rats, and its injury effect on gastroduodena is less than that of KP.

Objective To investigate the effect of long hairpin RNA ( lhRNA) expression vector targeting HBV X gene ( HBx) on replication of hepatitis B virus ( HBV) and gene expression.Methods Four kinds of small interference RNAs ( siRNAs) were synthesized and lhRNA expression vectors targeting HBx were constructed.Four siRNA oligonucleotides and two lhRNA expression vectors were transfected into HepG2.2.15 cells.HBsAg, HBV DNA in culture supernatants and HBx mRNA in HepG2.2.15 cells were detected by time-resolved immunofluorometric assay, real-time quantitative PCR, and reverse transcription PCR, respectively.Negative sequence group or empty vector group was taken as the control.Independent-samples t test was performed to evaluate the inhibition effect on replication of HBV and gene expression. Results Compared with the negative control, HBsAg, HBV DNA level in culture supernatants and HBx mRNA in HepG2.2.15 cells were significantly decreased after siRNA-1 and siRNA-4 transfected at high concentrations (60 nmol/L or 90 nmol/L) (P<0.05), especially the HBsAg and HBV DNA levels in the siRNA-1 transfection group, which were significantly decreased at 24, 48 and 72 h after transfection ( P<0.05 or P <0.01 ) . Two lhRNA expression vectors ( pMD-HBxlh1 and pMD-HBxlh4 ) were successfully constructed and transfected into HepG2.2.15 cells, HBsAg and HBV DNA level in transfected cells was significantly lower than those in negative control (P<0.05).Conclusion The novel siRNA-1 is confirmed to target HBx gene and lhRNA expression vector targeting HBx can effectively inhibit the replication of HBV and expression of HBV gene.

Objective:To investigate the effects of different doses of rosuvastatin on lipid levels in elderly patients with CHD complicated with hyperlipidemia.Methods:120 elderly patients with coronary heart disease admitted in our hospital were retrospectively chosen.By lipid examination,they were all hyperlipidemia.According to the dose of rosuvastatin,they were divided into three groups,and 40 cases in each group.They were given 5 mg,10 mg and 20 mg treatment,respectively.After 2 months,the changes of blood lipid level and the clinical effective rate were compared before and after the treatment.Results:①The blood lipid levels HDL-C [(1.17 ± 0.62) mmol / L] was significantly higher than the other two groups.LDL-C [(3.67 ± 0.83) mmol / L],TC [(4.36 ± 0.96) mmol / L] and TG [(1.68 ± 0.94) mmol / L] were significantly lower than those of the other two groups (P ＜0.05).②The effective rate of treatment in the 20mg group (97.5％) was significantly higher than that in the 5mg group (87.5％) and the 10mg group(85.0％).Conclusions:Rosuvastatin can treat coronary heart disease complicated with hyperlipidemia in elderly patients.The dosage of 20 mg can improve the blood lipid level of the patients,is an effective and convenient method,and with high therapeutic efficiency.

OBJECTIVE: To discuss the new work mode of clinical pharmacists participating in ward round and to promote rational use of antibiotics. METHODS: The status quo of clinical pharmacists participating in analysis of antibacterials used before and after ward round was analyzed statistically. RESULTS: After pharmacists participating in ward round, the cost ratio of antibiotics to total drugs was decreased by 5.56%. Rationality of antibacterial used in perioperative period or by infectious patients was improved significantly. CONCLUSIONS: The work mode of clinical pharmacists participating in ward round is effective and feasible, which plays a key role in promoting rational use of antibacterial and medical quality.

AIM　To observe the preventive action of leflunomide (Lef) on CCl4-induced liver fibrosis and explore its mechanism. METHODS Model of liver fibrosis in mice was induced by subcutaneous injection of CCl4(20%). The levels of ALT、AST、NO in plasma and Hyp in liver tissue were determined by spectroscopy. The content of HA in plasma was determined by radioimmunoassay. RESULTS Pretreatment of Lef (4,12,36 mg·kg-1) significantly reduced the elevated levels of ALT、AST、NO in plasma and Hyp in liver tissue, Pathological examination suggested Lef has preventive action on experimental liver fibrosis with significance. CONCLUSION Lef has significantly preventive action on CCl4-induced liver fibrosis possibly mediated by reduction of NO.

AIM　To study the therapeutic action of leflunomide(Lef) on adjuvant arthritis (AA) rats and its mechanisms. METHODS　To observe the change of secondary inflammation,immune function on adjuvant arthritis(AA) rats treated with Lef and to detect the effect of A771726—the active product of Lef on T cell subgroup in mice in vitro.RESULTS　Lef(2,6,18 mg*kg-1×12 d) intragastric injection(ig) could signifcantly inhibit secondary reaction (secondary inflammatory swelling、 multiple arthritis) of AA rats.Lef couldnt effect the lowed response of Con A-induced splenolytes, and the decreased IL-2 synthesis in AA rats. But lef(2,6,18 mg*kg-1) could inhibit the elevated IL-1 released from peritoneal macrophage (PMΦ) in AA rats. A771726—the active product of Lef(0.1，0.5，2.5，12.5，25，100 μmol*L-1) could inhibit Con A-induced Th cells but has no effect in Con A-induced Ts cells in vitro.CONCLUSION　Lef has therapeutic action on AA rats which could be related to its immunosuppressory activities——mainly through inhibit cell-mediated immunity.