Objective To investigate the correlation between the orthodontic pain score and occlusal function at 24 h after the first time treatment in the patients with fixed orthodontics.Methods Seventy-eight voluntary subjects were selected and treated with MBT straight wire correcting appliance.The VAS scores of spontaneous odontalgia and biting odontalgia (SO-VAS and BOVAS),masticatory efficiency (ME),masticatory times (MT),maximal occlusal force(MF)and occlusion rate before wearing the fixed appliance(T0),at 24 h after wearing(T1) were measured.The changes of above indicators were observed and the correlation among indicators was investigated:Results (1) BO-VAS and SO-VAS were increased after 24 h of orthodontic treatment,while BO VAS was (59.49 ± 19.06) mm,which was significantly higher than SO-VAS[(23.21± 20.80) mm].After 24 h of fixed orthodontic treatment,the patients had occlusal dysfunction,which manifested by ME,MT,MF and SO decrease compared with T0 (P＜0.05).(2) BO-VAS was negatively correlated with ME,MT and MF (P＜0.05).There was no correlation between SO-VAS and occlusal dysfunction.Conclusion There is no obvious magnitude correspondence relation between the occlusal dysfunction grade and the pain grade on the first day after fixed orthodontic treatment,the VAS grading is not yet fully representative of the occlusal dysfunction level,but the credibility of BO-V.AS score is higher than that of SO-VAS score in assessing the degree of occlusal dysfunction.

Objective:To observe the clinical parameters and short-term prognosis of Tibetan high-altitude area children with congenital heart disease undergoing surgery and anaesthesia in low-altitude area,and to investigate the perioperative safety of the treatment.Methods:From January,2016 to December,2016,14 children with congenital heart disease who underwent surgery were assigned into 2 groups (n=7 each):the high-altitude area group (X group,children from Tibetan Autonomous Region) and the low-altitude area group (H group,children from Hunan Province).Echocardiography data,perioperative hemodynamic changes,postoperative recovery,complication and perioperative serum N terminal pro B type natriuretic peptide (NT-proBNP) levels were recorded.Results:There were no significant differences in cardiac structure and function between the 2 groups,while the incidence of pulmonary hypertension in the X group was significantly higher than that in the H group (P＜0.05).There were no significant differences in perioperative hemodynamics between the 2 groups (P＞0.05),while the duration in ICU in the X group was longer than that in the H group and the serum NT-proBNP level in the X group was higher than that in the H group (P＜0.05).Conclusion:For children with congenital heart disease in Tibetan high-altitude area,undergoing surgery in low-altitude area contributes to a steady perioperative hemodynamics and helps to increase the perioperative safety.There may be a higher risk of postoperative cardiac dysfunction in Tibetan children than that in low-altitude area.

Objective To explore the effects of behavioral activation (BA) intervention on patients with chronic obstructive pulmonary disease (COPD) complicated with anxiety and depression. Methods Totally 70 patients with COPD complicated with anxiety and depression who were hospitalized in a ClassⅢ Grade A hospital from August 2018 to April 2019 were selected using convenient sampling and divided into experimental (n=35) and control (n=35) groups according to the time of admission. Patients in the control group received conventional hospital care and psychological guidance in the Department of Respiratory Medicine, while patients in the experimental group received BA intervention on this basis. Anxiety, depression and fatigue scores were compared between the two groups. Results A total of 33 patients in control group and 32 patient in experimental group completed the study. After BA intervention, the anxiety and depression scores of the experimental group were lower than those of the control group, and there were statistically significant differences between the two groups (P＜0.05). The total score of multi-dimensional fatigue scale of COPD patients in the experimental group was lower than that of the control group, the difference was statistically (P＜ 0.05), and there was no statistically significant difference in the score of mental fatigue between the two groups (P＞0.05). Conclusions BA intervention can ameliorate the anxiety and depression in patients and reduce their sense of fatigue, thus improving their quality of life.

Objective To investigate the optimal timing of indomethacin administration on prevention of post-endoscopic retrograde cholangiopancreatography ( ERCP ) pancreatitis. Methods The patients were randomly divided into the preoperative group and the postoperative group, and given 100 mg indomethacin in rectum within 30 min before and after ERCP, respectively. The serum levels of amylase and lipase were measured preoperatively and 4 h, 24 h postoperatively. The incidences of postoperative complications such as acute pancreatitis, hyperamylasemia, gastrointestinal bleeding, and perforation were assessed. Results A total of 340 patients were enrolled in this study from June 2014 to June 2017. The preoperative group consisted of 163 patients, including 11 cases ( 6. 75％) with post-ERCP pancreatitis ( PEP ) and 32 cases ( 19. 63％) with hyperamylasemia. Meanwhile, 177 patients constituted the postoperative group, with 25 cases ( 14. 12％) with PEP and 55 cases ( 31. 07％) with hyperamylasemia. The incidences of PEP and hyperamylasemia were significantly lower in the preoperative group than that in the postoperative group ( P = 0. 027 and P = 0. 016, respectively ) . Conclusion Preoperative use of indomethacin can better reduce the incidence of PEP than postoperative administration, without incidence increase of other complications.

Objective To construct the Hut78 cell line with EZH2 gene knocked into by CRISPR/Cas9 system. Methods The EZH2 expression vector pMD-18T-EZH2 with homologous arm and the sgRNA expression vector pSpCas9 (BB)-2A-Puro-sgRNA, which could cut the double stranded genomic DNA, were constructed, and the two vectors were co-transfected into Hut78 cells. Then the expression of EZH2 mRNA was detected by qPCR, and the expressions of EZH2 and H3K27me3 proteins were detected by Western blot assay. Results The pMD-18T-EZH2 and pSpCas9(BB)-2A-Puro-sgRNA recombinant vectors were confirmed by DNA sequencing. When Hut78 cells were transfected with the two recombinant plasmid, qPCR results showed that the expression of EZH2 mRNA was significantly increased, and Western blot analysis showed that the expressions of EZH2 and H3K27me3 proteins were significantly increased. Conclusion EZH2 gene is successfully knocked into Hut78 cells by CRISPR/Cas9 system.