1. The relationship among depression, anxiety, stress and addictive substance use behavior in 5 935 secondary vocational students
Xiaodong YU ; Jincong YU ; Qingfeng WU ; Jiayan CHEN ; Yuncui WANG ; Dong YAN ; Shiwei TENG ; Yanting ZHAO ; Jiepin CAO ; Shuaiqi LI ; Yaqiong YAN ; Jie GONG ; Kang YAO ; Hui ZHOU ; Zengzhen WANG
Chinese Journal of Preventive Medicine 2017;51(3):226-231
Objective:
To investigate the relationship among depression, anxiety, stress and addictive substance use behavior in secondary vocational students.
Methods:
Cluster sampling method and the Adolescent Health-related Behaviors Questionnaire were used to collect demographic characteristics, psychological symptoms, and addictive substance usage among 5 935 students in nine vocational schools in Chongqing, Zhaoqing, Ningbo, and Taiyuan. Multivariate logistic regression analysis was used to analyze the relationship between the addictive substance use behavior and psychological factors.
Results:
The detection rates of depression, anxiety and stress were 46.5% (
2.Improvement of the caf1 based PCR method for detection of the plague
Yan ZHANG ; Ying GUO ; Shanshan DONG ; Yuncui JIE ; Youhong ZHONG ; Wei LI ; Zhizhong SONG ; Peng WANG
Chinese Journal of Endemiology 2018;37(3):203-206
Objective To identify the causes of nonspecific bands in the detection of a industry standard caf1 gene by polymerase chain reaction(PCR),and to propose a solution to this problem. Methods A total of 112 strains were selected for the experiment, including 40 strains of Yersinia pestis, 72 strains of non-Yersinia pestis;DNA was extracted,and caf1 gene was amplified by PCR;seven non-specific strips were recovered,purified and TA cloning and sequencing; the primer of the caf1 gene was redesigned and validated using all of the strains. Results Using the industry standard caf1 gene primer,DNAs of 40 Yersinia pestis and 72 non-Yersinia pestis were amplified by PCR, 58 non-Yersinia pestis could be amplified with non-specific bands, they were about 400, 500, 600, 700, 800, 900, 1 000 bp. By TA cloning and sequencing, the non-specific bands in the downstream of the industry standard caf1 primer and its reverse complement were amplified. Using the new designed caf1 primer to amplify, 72 non-Yersinia pestis strains showed no non-specific bands. Conclusion Non-specific bands has been amplified in the screening of Yersinia pestis using the primer of the industry standard caf1, and the new caf1 primer can effectively avoid this problem and improve the accuracy of detection.