Pre-implantation Genetic Diagnosis(PGD) is an advanced technology based on in-vitro fertility-embryo transfer,which binds multiple-discipline arts,specially the research on DNA analysis assay of single cell,to reach the aim of aristogenesis and wash out abnormal embryo before transfer.With the development of this technique and the ever wider applications,ethical issues also arise.This paper focuses on these ethical issues related to PGD,expecting an actual benefit for human.

Objective To improve the understanding of the radioactive charcteristics of vertebral eosinophilic granuloma.Methods The imaging features (X-ray?CT and MRI) of 10 cases with vertebral eosinophilic granuloma were retrospectively analyzed.Diagnoses of all the patients had been confirmed with pathlolgical examination after operation.Results All the patients had vertebral body damages,7 with osteolytic damages and 3 with cystic damages.Intervertebral crevice became narrowing or disappearing .Some vertebral appendix and paravertebral soft tissue were involved.Conclusion It is out of proportion among the vertebral body damages,the involved vertedral arch,the changes of intervertebral crevice and the mass of paravertebral soft tissue.The remaining bone fragment without sequestrum in the damage ares is considered as the main characteristic expression of vertebral eosinophilic granuloma.

Objective To compare the clinical results of the intramedullary nails (Gamma nail and the proximal femoral nail) in treatment of intertrochanteric fractures of femur. Methods A review study was conducted on 116 intertrochanteric fractures of femur treated with the Gamma nail and 89 treated with PFN in our hospital between 2000 and 2003. Results In the Gamma nail group, the mean time for operation was 68.3(48 to 106)minutes,the mean blood loss during operation was 261(180 to 400)mL,the mean time for walking after operation was 5.0 (2.3 to 8.9)weeks and the mean time for bone union was 8.6(7.1 to 12.6)weeks. In the PFN group, the mean time for operation was 48.0(36 to 85)minutes, the mean blood loss during operation was 192(120 to 360)mL,the mean time for walking after operation was 5.3(2.5 to 8.1)weeks and the mean time for bone union was 8.8(6.9 to 12.1)weeks. There were significant differences in the mean time for operation and the mean blood loss between the 2 groups(P

Humans ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; Rhinitis, Allergic, Seasonal

Objective: To analyze the correlation of carotid artery color Doppler flow imaging (CDFI) indices and transcranial Doppler ultrasound (TCD) indices. Methods: The clinical data of 118 hospitalized patients with ischemic cerebrovascular diseases were included in the present study and all of them received CDFI and TCD examination. The patients were divided into two groups according to presence of carotid plaque group; t-test was used to identify the correlation between the presence of carotid plaque and TCD indices. Meanwhile, the patients were also divided into three groups according to the quantity/distribution of plaque) one-way ANOVA(F-test) and student-Newman-Keuls were used to identify the correlation of the quantity/distribution of plaque with TCD indices. The patients with carotid plaque were divided into three groups according to the plaque texture) one-way ANOVA(F-test) and student-Newman-Keuls were used to identify the correlation of the quality of plaque with TCD indices. Meanwhile, linear correlation was used to identify the correlation of the common carotid intimal-medial thickness (CCA IMT) with TCD indices. Results: The resistance index (RI), pulsatile index (PI) and ratio of peak systolic to least diastolic flow velocity (S/D) of bilateral middle cerebral arteries and basal artery in patients with carotid plaque were significantly higher than those in patients without carotid plaque (P<0.05); other TCD indices including Vp, Vd, and Vm were not significantly different. The quantity/distribution of plaque, the quality of plaque and CCA IMT were not correlated with TCD indices. Conclusion: No CDFI indices, except for the presence of carotid plaque, are correlated with TCD indices. Combined application of CDFI and TCD indices can better reflect the structure and hemodynamic status of cerebral vessels.

Dermatology ; Humans ; Integrative Medicine ; Physicians

Objective To evaluate the status of the morbidity of serum lipids in Beijing through the detection of serum lipids in health checking adults.Methods The serum total cholesterol(TC),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C)and triglyceride (TG)were detected by chemistry test in 13 336 adults,and age-adjusted prevalence of dyslipidemia and its distribution in different sexes and age groups were statistically analyzed.Results The prevalence of total dyslipidemia is 59.9%,71.6 % in male and 47.2% in female.The prevalence of the four components of serum lipids raised with age in both sex(P

microRNAs function as effective molecules in regulation of many biological functions of organisms; in most case they regulate gene expression moderately. Emerging evidence suggests that microRNAs play a key role in the regulation of immunological functions including innate and adaptive immune responses. The research on microRNAs would be helpful in elucidation of the mechanisms of human immune system and in development of potential therapies based on microRNAs.
Gene Expression Regulation ; Humans ; Immune System ; physiology ; MicroRNAs ; genetics ; physiology

High performance liquid chromatography-time-off-flight mass spectrometer (HPLC-TOFMS) technology coupled with partial least squares discriminant analysis (PLS-DA) processed by SIMCA-P software was applied to investigate serum endogenous metabolites alternations of valsartan in spontaneous hypertension rats (SHR). And MetPA platform was used to connect identified potential biomarkers in corresponding metabolic pathways to find possible therapeutic mechanism of valsartan. Valsartan significantly declined the blood pressure of SHRs (P < 0.05) at fourth week. The metabolic profiling significantly changed and four metabolites involved in G protein-coupled pathway were identified. Metabolomics is able to detect holistic and microcosmic alternations in organism, so as to elucidate therapeutic mechanism of drugs.
Animals ; Biomarkers ; blood ; Blood Pressure ; Chromatography, High Pressure Liquid ; Discriminant Analysis ; Least-Squares Analysis ; Mass Spectrometry ; Metabolome ; Metabolomics ; Rats ; Rats, Inbred SHR ; Valsartan ; pharmacology

In the present work, Monte Carlo simulations were employed to study the characteristics of the dose distribution of high energy electron beam in the presence of uniform transverse magnetic field. The simulations carried out the transport processes of the 30 MeV electron beam in the homogeneous water phantom with different magnetic field. It was found that the dose distribution of the 30 MeV electron beam had changed significantly because of the magnetic field. The result showed that the range of the electron beam was decreased obviously and it formed a very high dose peak at the end of the range, and the ratio of maximum dose to the dose of the surface was greatly increased. The results of this study demonstrated that we could change the depth dose distribution of electron beam which is analogous to the heavy ion by modulating the energy of the electron and magnetic field. It means that using magnetic fields in conjunction with electron radiation therapy has great application prospect, but it also has brought new challenges for the research of dose algorithm.
Algorithms ; Electrons ; Humans ; Magnetic Fields ; Monte Carlo Method ; Phantoms, Imaging ; Radiation Dosage