2.Zhu Lian's cognition on theory and method of acupuncture and moxibustion under background of western medicine.
Su-yun LI ; Li-jian ZHANG ; Bing LIU
Chinese Acupuncture & Moxibustion 2014;34(11):1127-1130
With new acupuncture and moxibustion as the study object, based on the basic composition of acupuncture-moxibustion theory, from 3 aspects of meridian-acupoint theory, acupuncture-moxibustion method theory and acupuncture-moxibustion treatment theory, under the background of western medicine, ZHU Lian's different opinions on theory and method of acupuncture and moxibustion were discussed. It was believed by ZHU Lian that the distribution of 14-meridians was approximately identical to that of nerves, so with modern neuroanatomy knowledge to understand the meaning of acupoint; the acupuncture function could be explained from the angle of neurophysiology. Clinical diagnosis and treatment method could be established by modern classification methods of diseases. ZHU Lian's cognition that was different from traditional theory and method of acupuncture and moxibustion was combined with updated physiology and anatomy knowledge at that time, and was involved with Pavlov's advanced nerve theory, so she firstly put forward the opinion that acupuncture therapy can't work without the involvement of cerebral cortex.
Acupuncture Points
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Acupuncture Therapy
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history
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psychology
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Books
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history
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China
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History, 20th Century
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History, 21st Century
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Humans
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Knowledge
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Medicine
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Moxibustion
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history
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psychology
3.Preventive effect of hepatic arterial infusion (DDS) against post-operative recurrence of hepato-cellular carcinoma
Jian-Min GUO ; Yun-Li ZHANG ; Li-Xing ZHOU ;
China Oncology 2001;0(05):-
Purpose:To explore the preventive effect of hepatic arterial infusion (DDS) against recurrence of hepato- cellular carcinoma (HCC) after radical resection.Methods:From Jan,1996 to May,1998,287 patients of HCC after radical resection were randomly divided into four groups:intra-hepatic arterial infusion(97),intra-portal vein(80),intra-hepatic artery and portal vein (60),control (50).All patients received chemotherapy for two years and observed for postoperative recurrence of HCC.Results:The postoperative recurrence rate of HCC with intra-hepatic arterial infusion was significantly lower than that of intra-portal vein (P0.05).The 1~、2~ and 3~year survival rate of intra-hepatic arterial infusion was significantly higher than any of the other groups.Conclusions:Intra-hepatic arterial infusion (DDS) through gastro-duodenal artery can effectively pro- long the postoperative survival and decrease the post-operative recurrence rate of HCC.The preventive method of DDS through gastro-duodenal artery was safer and effective.
4.Current status of research on target delineation of partial breast external irradiation after breast-preserving surgery of early breast cancer patients.
Yun DING ; Wei WANG ; Jian-bin LI
Chinese Journal of Oncology 2013;35(12):881-885
Breast Neoplasms
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pathology
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radiotherapy
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surgery
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Cone-Beam Computed Tomography
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Dose Fractionation
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Female
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Humans
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Mastectomy, Segmental
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adverse effects
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Neoplasm Staging
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Radiotherapy Planning, Computer-Assisted
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methods
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Radiotherapy, Conformal
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methods
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Respiration
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Seroma
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etiology
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pathology
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Surgical Instruments
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Tumor Burden
5.Activation of protease-activated receptor 2 inhibits apoptosis of lung cancer cells
Shaohong HUANG ; Jun AN ; Yun LI ; Junhang ZHANG ; Jian RONG
Chinese Pharmacological Bulletin 2014;(5):684-687,688
Aim To investigate the activation of prote-ase-activated receptor 2 ( PAR2 ) in regulation of the expression of epidermal growth factor receptor ( EGFR) and apoptosis of lung cancer ( LC) cells. Methods LC cells A549 and its EGFR-silenced counterpart were incubated in the medium added with tryptase. Quanti-tative RT-PCR and Western blotting were used to de-tect the expression of EGFR in A 5 4 9 cells . The apop-tosis and Bax/Bcl-xL of cells were also recorded. Re-sults Treating A549 cells with tryptase in the culture for 48 hrs resulted in a marked increase in the expres-sion of EGFR in A549 cells. Marked decreases in a tryptase dose-dependent manner in apoptotic A549 cells were detected in the presence of tryptase. Expo-sure to tryptase markedly decreased the levels of Bax and increased the levels of Bcl-xL in A549 cells, which were not shown in EGFR-deficient A549 cells. Conclusion Tryptase can increase the expression of EGFR in LC cell line, A549 cells, which protects A549 cells from apoptosis, increases Bcl-xL, and sup-presses Bax in A549 cells.
6.Infectious Cloning Approach for Herpesvirus Based on Bacterial Artificial Chromosomes
Jian-Hong LU ; Yun-Lian TANG ; Gui-Yuan LI ;
China Biotechnology 2006;0(06):-
The genetic analysis of herpesviruses has been a constant challenge, due to the large, complex genomes of herpesviruses and mutagenesis of viral genes by conventional recombination methods in cell culture. Recently, a completely new approach for full-length infectious clones of herpesviruses based on bacterial artificial chromosomes (BACs) has been developed. This technique allows the maintenance, propagation and genetic modification of the viral genome as a BAC plasmid in E.coli, thus making the procedures fast, safe and effective in prokaryotic cells. This technique also makes it possible for the reconstitution of viral progeny or mutants by transfection of the BAC plasmid into eukaryotic cells, thereby facilitating the analysis of viral gene functions in the context of genome. In this presentation, Epstein-Barr virus was used as an example to describe the principle, establishment of the technique and mutation introduction into the BAC plasmid, and to discuss the perspective in the use of BAC-cloned herpesviruses.
7.Analysis of monitoring results of plague from 2002 to 2010 in Ejinaqi of Alasham
Xue-mei, YIN ; Jian-yun, LI ; Fang, LIU
Chinese Journal of Endemiology 2012;31(5):545-547
Objective To findout the current situation of plague,and the population structure and density distribution of rodent.Methods According to The Plague Monitoring Scheme of Inner Mongolia,we surveyed the surrounding areas of Ceke and Xinximiao.The density of rats was surveyed by one-day bow-clip method and the method of 5 meters clamp law catching rats was used.According to The Plague Diagnostic Criteria(WS 279-2008),the rats and fleas were detected by isolation and culture of Yersinia pestis,the serums of rodent were tested by indirect hemagglutination test.Results From 2002 to 2012,the monitored area was 291 km2.The number of qnadrat in every square kilometers was 118.The number of rodent captured was 1051,the number of Meriones Meridianus was 526,accounting for 50.05% (526/1051),the number of Euchoreutes Naso was 175,accounting for 16.65% (175/1051),and the number of Rhombomys Opimus was 154 accounting for 14.65%(154/1051).The average rodent density was 8.91/hm2.Etiology and serological test results were negative,46 groups of 753 fleas were cultured and 154 serum samples were tested.Conclusions There is no epidemic situation of animal plague in this area,but Meriones Meridianus is a dominant rat with high resistant.So,intensive monitoring should be strengthened in this area to prevent the prevalence of rodent and human plague.
9.Synthesis of glycylproline p-nitroanilide tosylate and its clinical application
li-yun, YAO ; jian-hua, ZHANG ; rong, WANG ; yang, LU
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(04):-
Objective To synthesize glycylproline p-nitroanilide tosylate as a substrate for glycylprolyl dipeptidyl-aminopeptidase(GPDA),and to study its clinical application.Methods The title compound was prepared by DCC-HOBt synthesis.GPDA in human serum was detected by continuous monitoring method using substrate. Results The substrate with purity of 98.5% was obtained.The linearity of the method was up to 358.1 U/L.Intraassay CV and interassay CV of GPDA in diverse serum samples were 3.01% and 5.04%,respectively.It was shown that GPDA level in patients with hepatic cancer was significantly increased,while those in patients with gastric carcinoma and chronic gastritis were significantly decreased compared with that in the control group in clinical detecting(P
10.Screening of the Microbe Producing Docosahexaenoic Acid from Seawater and Identification on a Strain of High Yield
Yu-Qiao ZHAO ; Yun-Jian DU ; Li-Yan WANG ;
Microbiology 1992;0(04):-
Microbes that produce Docosahexaenoic Acid were isolated from seawater. 160 strains capable of producing lipids were screened out using Sudan Black B dying method from 280 seawater samples. From 60 strains of microorganisms producing bigger lipid particles, 7 strains of them capable of producing lipids more than 8% were obtained with Soxhlet abstracting method in the first screening. In the secondary screening from 10 strains with high lipids yield, strain 7-3 capable of producing 15.9% lipids was obtained, in which the content of DHA(Docosahexaenoic Acid)is 45.2%. Strain 7-3 was identified as Brettanomyces based on its morphological properties, cultural characteristics, physiological and biochemical properties.