Objective To investigate the effects of angiotensinⅡ(AngⅡ) receptors antagonist on the LPS-induced acute lung injury (ALl).Method Wistar rats were randomly divided into three groups:control group;in LPS group,mrs were treated with LPS (10 mg/kg) at 3,6,12,and 24 hours;in LPS+ AngⅡreceptors antagonist group,pre-expasure to AngⅡreceptors antagonist [Sar~1,Ile~8] AngⅡfor 30 minutes before treated with LPS (10 mg/kg) for 6 hours.All the rats were killed,and the lung tissue was collected.Results Compared with control group pulmonary wet/dry weight ratio was significantly higher in the other two groups (P

Administration, Topical ; Ear, Inner ; pathology ; Humans ; Meniere Disease ; therapy

No abstract available.
Heart Septal Defects, Ventricular* ; Pulmonary Valve Stenosis*

0.05). Conclusion Oral administration of prednisone can prevent modrate and severe OHSS and may have no effect on pregnant rate of IVF.

Adolescent ; Adult ; Aged ; Antifungal Agents ; therapeutic use ; Drug Therapy, Combination ; Echinocandins ; administration & dosage ; therapeutic use ; Female ; Hematologic Neoplasms ; drug therapy ; microbiology ; Humans ; Lipopeptides ; Male ; Middle Aged ; Mycoses ; drug therapy ; Pyrimidines ; administration & dosage ; therapeutic use ; Treatment Outcome ; Triazoles ; administration & dosage ; therapeutic use ; Voriconazole ; Young Adult

Aconitum brachypodum is traditionally known to be toxic chinese medicie, but its chemical constituents is not enough studied to date. To further elucidate the chemical constituents of A. brachypodum, 80% ethanol extract of A. brachypodum collected from Dong-Chuan area was investigated, which led to isolation of seventeen compounds. By spectroscopic methods, their structures were determined as hypaconitine (1), mesaconitine (2), talatisamine (3), neoline (4), fuziline (5), aconine (6), bullatine A (7), lepeine (8), songrine (9), isocorydine (10), beta-sitosterol (11), daucosterol (12), stearic acid (13), triacontanol (14), palmitic acid (15), benzoic acid (16), and inosine (17), respectively. All compounds except for compounds 1 and 7 were isolated from A. brachypodum for the first time.
Aconitum ; chemistry ; China ; Drugs, Chinese Herbal ; chemistry ; Magnetic Resonance Spectroscopy

Objective: To investigate the prevalence of scoliosis among primary and middle school students in Jiaxing City, Zhejiang Province, so as to provide insights into scoliosis control among children and adolescents. Methods: Grade 4 to 6 primary school students and grade 1 to 3 junior high school students were recruited using a stratified cluster sampling method in Jiaxing City in 2019. Participants' demographic characteristics, dietary habits and nutritional status, physical activity, learning environments, reading and writing habits were collected using questionnaires. Scoliosis was screened through general examinations, forward bend test and scoliometer, and scoliosis was diagnosed with whole-spine X-ray scans in an erect position. The prevalence of scoliosis was descriptively analyzed among primary and middle school students. Results: A total of 8 026 primary and middle school students were included, 7 304 valid questionnaires were recovered, with an effective recovery rate of 91.00%. The respondents included 3 667 primary school students (50.21%) and 3 637 junior high school students (49.79%), and included 3 776 boys (51.70%) and 3 528 girls (48.30%). There were 659 participants with initial screening positive for scoliosis (9.02%), and the percentages of positive initial screening of thoracic, thoracolumbar and lumbar scoliosis were 2.93%, 4.56% and 4.56%, respectively. A higher percentage of positive initial screening of scoliosis was diagnosed among participants living in Pinghu City (10.45%), junior high school students (11.74%), girls (11.96%), students with a medical history of anemia (22.44%), students with less than 3 days of moderate-intensity physical activity in the past week (9.46%), students with less than 3 days of walking duration of over 10 minutes in the past week (10.18%), students with daily sitting duration of 5 hours and more in the past week (10.74%), students with their class seats exchanged every semester or month (10.28%), students with daily reading and writing duration of 3 hours and more after school (10.93%) and students with less than 10 cm distance from the chest at reading or writing to the edge of the table (9.67%) (all P<0.05). A total of 218 students received whole-spine X-ray scans in an erect position, 132 participants were definitively diagnosed as scoliosis (60.55%), and the estimated prevalence of scoliosis was 5.46%. Conclusion The percentage of positive initial screening of scoliosis was 9.02% among primary and middle school students in Jiaxing City. Gender, stage of learning, nutritional status, exercise frequency and habits of reading and writing may be factors affecting the development of scoliosis.

AIM: To get high biological activity of recombinant human bone morphogenetic protein -2 (rhBMP-2) expressed in Escherichia coli by the methods of refolding. METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells. RESULTS: The rhBMP - 2 was expressed in Escherichia coli in a non - active aggregated form of inclusion bodies using a temperature - inducible expression system. The high - purified rhBMP - 2 was obtained in the form of inclusion bodies by several purification courses. The rhBMP - 2 was refolded and dimerized in the redox systems (reduced and oxidized glutathione) and a one - step purification procedure based on the heparin affinity chromatography was implemented to isolate the rhBMP - 2 dimers and monomers. The purified rhBMP - 2 dimers showed the higher biological activity than the commercial rhBMP - 2. CONCLUSIONS: The method achieved the refolding of rhBMP - 2 would be applied to the whole TGF - β superfamily because the BMP - 2 belongs to the superfamily. Meanwhile, the inexpensive,high yield rhBMP - 2 is suitable for clinic application.

AIM: To get high biological activity of recombinant human bone morphogenetic protein-2 (rhBMP-2) expressed in Escherichia coli by the methods of refolding. METHODS: The rhBMP-2, expressed in Escherichia coli, was washed by Triton X-100 and further purified by DEAE chromatography. The inclusion bodies were resolved in 8 mol/L urea,and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one-step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP-2 were tested by induction of the alkaline phosphatase activity in C2C12 cells. RESULTS: The rhBMP-2 was expressed in Escherichia coli in a non-active aggregated form of inclusion bodies using a temperature-inducible expression system. The high-purified rhBMP-2 was obtained in the form of inclusion bodies by several purification courses. The rhBMP-2 was refolded and dimerized in the redox systems (reduced and oxidized glutathione) and a one-step purification procedure based on the heparin affinity chromatography was implemented to isolate the rhBMP-2 dimers and monomers. The purified rhBMP-2 dimers showed the higher biological activity than the commercial rhBMP-2. CONCLUSIONS: The method achieved the refolding of rhBMP-2 would be applied to the whole TGF-? superfamily because the BMP-2 belongs to the superfamily. Meanwhile, the inexpensive, high yield rhBMP-2 is suitable for clinic application.

OBJECTIVETo study the anti-asthmatic effect of sodium ferulate (SF) and its mechanism in guinea pig asthmatic model.

METHODSGuinea pigs were sensitized with ovalbumin as animal asthmatic model and treated with 3 different concentration of SF for 8 days. Levels of endothelin (ET) and nitric oxide (NO) in blood and lung tissue, and eosinophil (EOS) in blood and bronchoalveolar lavage fluid (BLAF) was counted at the end of trial.

RESULTSSF could significantly lower the ET content and increase the NO concentration in serum and lung tissue, reduce the number of EOS in blood and BLAF (P < 0.05, P < 0.01). Stronger effect was showed in the high dose group.

CONCLUSIONMechanism of anti-asthmatic action of SF might be to increase NO concentration, lower ET content, alleviate EOS infiltration.

Animals ; Anti-Asthmatic Agents ; pharmacology ; Asthma ; blood ; chemically induced ; Bronchoalveolar Lavage Fluid ; cytology ; Coumaric Acids ; pharmacology ; Endothelin-1 ; blood ; Eosinophils ; pathology ; Female ; Guinea Pigs ; Male ; Nitric Oxide ; blood ; Ovalbumin